Effects Of Nutritional Manipulation Research Articles

Effects of nutritional manipulation on body composition in the developing marsupial, Macropus eugenii

When 60-day-old tammar wallaby pouch young (Macropus eugenii) are fostered to mothers at 120 days of lactation, their growth, developmental rate and maturation of their GH/IGF axes are markedly accelerated. To determine the effect of fostering on energy intake, body composition and fat accretion, we first measured total body fat and lean mass in these young. Next, we mimicked the triglyceride oleic and palmitic acid composition of 120-day milk by supplementing 60 day young with these fatty acids and comparing their growth with that of growth accelerated young. There was no difference in the weight or growth axis maturation of supplemented young but there was significantly more body fat in these and in the growth-accelerated fostered young than in controls. We conclude that the accelerated growth and GH/IGF axis maturation observed previously in fostered young is most likely due to increased milk consumption and earlier access to specific nutrients.

Grasa y proteína de la leche de vaca: componentes, síntesis y modificación

El objetivo de este trabajo fue realizar una revisión de los componentes lipídicos y proteicos de la leche de vaca, ya que los ácidos grasos, las caseínas, y las proteínas del lactosuero, entre otros, poseen una importancia significativa dentro de la nutrición humana. En este ámbito, el consumidor elige entre uno u otro componente, influyendo de manera directa sobre la oferta y la demanda de productos lácteos. Esta dependencia del mercado hacia el consumidor, exige al profesionista involucrado en la producción lechera, una mayor comprensión del suministro de nutrientes a la glándula mamaria, con el fin de adquirir herramientas que le permitan modificar la composición de la leche conforme a las exigencias de la industria láctea. Por lo tanto, se presenta una revisión que enfatiza la utilidad de modelos esquemáticos en procesos metabólicos, para incrementar nuestro conocimiento científico sobre factores como la elongación y desaturación de los ácidos grasos, la transcripción y traducción proteica, y la formación de glóbulos grasos y micelas de caseína, elementos teóricos de vital importancia si se busca alterar la grasa y la proteína láctea. Cabe señalar, que este documento se ha desarrollado con un enfoque bioquímico, lo que le otorga originalidad y lo distingue de otras revisiones enfocadas exclusivamente en la modificación de aspectos nutricionales, y su efecto sobre los componentes de la leche. Adicionalmente, son muy escasas las revisiones actualizadas que abordan esta temática para estudiantes y profesionistas de habla hispana. Finalmente, se analizan reportes científicos y técnicos que consideran la manipulación nutricional, el efecto de la raza, la modificación del ambiente ruminal, y la variabilidad genética, sobre el contenido y la composición química de los lípidos y proteínas de la leche de vaca.

Effect of nutritional manipulation on brain function: implications for future research

Effect of nutritional manipulation on brain function: implications for future research

The Effect of Nutritional Manipulation on Ultra-Endurance Performance: A Case Study

The Atlantic Rowing Race requires teams of two to cover 3,000 nautical miles over 40–90 days. During this ultra-endurance event, competitors require substantial energy intake to meet metabolic requirements; therefore, sufficient physiological and nutritional support is paramount. Two highly trained males (aged 46) engaged in two 14d dietary interventions, with a 14d recovery period in between, to investigate the effect of such interventions on physiological (cardiovascular, cardiorespiratory, and blood-based measures) and performance-based (distance and split time) parameters during an ultra-endurance (2h on 2h off, for 24h) laboratory-based rowing protocol at 60% VO2max. Diet 1: high fat (HF) [60% fat, 30% carbohydrate and 10% protein] and Diet 2: high carbohydrate (HC) [20%, 70% and 10% respectively]. A greater distance was rowed by both subjects (155, 329m and 134, 797m vs 130, 089m and 122, 112m) with a concomitant reduced heart rate, volume of oxygen uptake, and respiratory exchange ratio, following the HF as opposed to HC dietary intervention. In summary, ultra-endurance performance was enhanced following a 14d HF diet, without apparent implications on liver function and overall lipid profile.

Effect of nutrition during pregnancy on fetal growth and survival in Fec B Booroola × South Australian Merino ewes

The effect of nutritional manipulation during mid and late pregnancy on fetal growth and survival in high fecundity (Fec B Fec +) Merino ewes was examined. A total of 4 treatment groups was utilized in each of 3 years (1987, 1988 and 1989); and 2 groups were given low nutrition during mid pregnancy while 2 groups received high nutrition during this period. One group from each of these treatments subsequently received a high protein diet 1 to 2 weeks prior to term. Differential feeding during mid pregnancy resulted in the high and low treatment groups varying by 7.5 and 5.0 kg liveweight at Days 100 and 135 of pregnancy, respectively. Fetal survival was not improved by variation in nutritional intake during mid pregnancy (P>0.05) and neither were placental and fetal growth (P>0.05), as indicated by observations on cotyledon diameter and fetal head width, respectively. However, fetal survival during late pregnancy was reduced by a high level of nutrition during both mid and late pregnancy (P<0.05).

Effect of Prolonged Modified Fasting in Obese Persons on In Vitro Markers of Immunity: Lymphocyte Function and Serum Effects on Normal Neutrophils

The effects of nutritional manipulation on immune function have been extensively studied in animals, but few studies have examined dietary restriction in humans. Obese patients enrolled in a protein-sparing, calorically restricted diet were monitored over a 3-month period with in vitro examination of mitogen- and antigen-induced lymphocyte blastogenesis. The sera from these patients were evaluated for effects on neutrophil chemotaxis, phagocytosis and microbial killing. Significant changes in body weight, triglycerides and glucose occurred during the diet, and most patients exhibited urinary ketosis. The diet was associated with increased blastogenesis in unstimulated cultures and in varicella and candida antigen-stimulated cultures, but blastogenesis was unchanged for phytohemagglutinin, concanavalin A, SK-SD and histoplasma. In assays of serum effects on neutrophil function, patients with urinary ketosis had depression of chemotaxis and microbial killing but not phagocytosis when compared to baseline or nonketotic patients. This study indicates that long-term caloric restriction is associated with significant effects on in vitro lymphocyte stimulation and with significant serum effects on normal neutrophil function.

31P NMR study of the impact of dietary manipulation on tumor metabolism and response to methotrexate.

The effects of nutritional manipulation and subsequent chemotherapeutic treatment upon growth and metabolism of a transplanted rat rhabdomyosarcoma were investigated by in vivo 31P NMR spectroscopy. Nutritional manipulation was accomplished by administration of a protein deprived diet containing no protein and 75.5% glucose. After 5 days the protein deprived rats (PD rats) were nutritionally replenished with a normal protein diet containing 27% protein and 47.3% glucose. Twenty-four hours after nutritional replenishment the PD rats and continuously well-fed controls (NP rats) received methotrexate (MTX, 30 mg/kg, i.p.). 31P NMR spectroscopy of the tumors 24 h after MTX administration showed a decreased ratio of nucleoside triphosphates to inorganic phosphate (referred to as 'ATP/Pi ratio') in PD rats in contrast to an unchanged ATP/Pi ratio in the NP controls. At the time of MTX administration the PD rats had a significantly lower tumor pH than the NP group (6.75 +/- 0.03 [SEM] vs 6.95 +/- 0.04; p less than 0.02). Tumor response in the PD group was significantly (p less than 0.01) enhanced compared to the NP group. These findings indicate that a period of dietary protein deprivation combined with a high glucose load and followed by nutritional replenishment impairs tumor metabolism. The altered metabolic status is expressed by acidification of the tumor and distinct changes in ATP/Pi ratio and appears to relate to an enhanced susceptibility to MTX chemotherapy.

Quantitative changes in adipocyte plasma membrane in response to nutritional manipulations.

The effects of changes in adipocyte size and the effects of nutritional manipulations on the quantity of plasma membrane per adipocyte were investigated. A method for estimating the quantity of plasma membrane was developed based on the specific labeling of adipocyte plasma membrane protein with the nonpermeable labeling agent 125I-labeled diazotized diiodosulfanilic acid. By studying rats (ranging in age from 50 to 125 days) fed a standard laboratory chow or a low fat diet or a high fat diet, a wide range of mean fat cell sizes was obtained. It was found that as the volume of the fat cell increased, the amount of plasma membrane increased in a linear fashion and that this linear relationship had the same slope whether the size of the adipocyte increased slowly with age or rapidly in response to a high fat diet. In contrast, fasting for up to 3 days caused a marked decrease in the mean volume of the adipocytes, but either no change or much less change in the amount of plasma membrane per cell than would have been predicted from the linear relationship between adipocytes, but either no change or much less change in the amount of plasma membrane per cell than would have been predicted form the linear relationship between adipocyte volume and amount of plasma membrane per cell obtained with fed rats, i.e., adipocytes from fasted rats contain more plasma membrane per cell than do fat cells of the same size from fed rats. Neither feeding a high fat diet nor fasting caused detectable changes in the protein and lipid composition of the adipocyte plasma membrane.

Tumor growth in experimental animals: nutritional manipulation and chemotherapeutic response in the rat.

The effects of nutritional manipulation on host body weight dynamics, tumor growth patterns and host-tumor responses to chemotherapy were studied in Sprague-Dawley rats with Walker-256 carcinosarcomas. Group I maintained throughout on a regular diet (RD) gained carcass weight steadily. Group II lost carcass weight while fed a protein-free diet (PFD) but rapidly gained weight after switching to RD on day 15. Mean tumor volume increased 105% in Group I from day 15 to 21, 218% in Group II and 77% in Group III (continued on PFD p less than 0.05). From day 21 to day 33 tumor growth patterns were similar in Groups I and II, while mean tumor volume eventually plateaued in Group III. In Study B, Group II animals were given Methotrexate (MTX-20 mg/kg) two days and six days after switching from PFD to RD. The mean change in tumor volume in the MTX-treated rats was 1.31 +/- 0.1 cm3 compared with 8.14 +/- 0.1 cm3 (p less than 0.001) in the saline-treated control rats. MTX did not significantly affect tumor growth patterns in Group III (PFD) rats. In Study A, protein-calorie malnutrition resulted in host carcass weight loss and tumor growth retardation while nutritional repletion restored host carcass weight and stimulated tumor growth. In Study B, MTX was maximally effective in tumor-bearing rats that were switched from PFD to RD demonstrating that nutritional manipulation can improve host nutritional status and increase tumor response to chemotherapy.