Effect Of Glucose Levels Research Articles

Metabolic stressful environment drives epigenetic modifications in oviduct epithelial cells

The oviduct provides a suitable microenvironment from the gametes’ final maturation until initial embryo development. Dynamic functional changes are observed in the oviduct cells, mainly controlled by steroid hormones and well-orchestrated during the estrous cycle. However, based on the roles played by the oviduct, additional layers of complexity might be present in its regulatory process. There is a cellular process that includes metabolic adaptation that can guide molecular modifications. This process is known as metaboloepigenetics. Therefore, we aimed to better understand how this crosstalk occurs in oviductal epithelial cells (OEC). Due to limited in situ access to the oviduct, we used the primary in vitro cell culture as a culture model and glucose as a metabolic disturbed factor. For that, cells derived from the oviductal epithelial layer were collected from cows at either follicular or luteal stages (n = 4 animals per group). They were cultured on a monolayer culture system under normoglycemic (2.7 mM glucose) or hyperglycemic conditions (27 mM glucose). On day five of culture, attached cells were submitted to analysis of mitochondrial metabolism (mitochondrial membrane potential - MMP) and epigenetics markers (5- methylcytosine - 5 mC and histone 3 lysine 9 acetylation - H3K9ac). Moreover, the culture media were submitted to the metabolites analysis profile by Raman spectrometry. Data were analyzed considering the effect of glucose level (normoglycemic vs. hyperglycemic), stages when OEC were harvested (follicular vs. luteal), and their interaction (glucose level * cycle stage) by two-way ANOVA. As a result, the high glucose level decreased the H3K9ac and MMP levels but did not affect the 5 mC. Regardless of the metabolic profile of the culture media, the glucose level was the only factor that changed the Raman shifts abundance. Although this present study evaluated oviductal epithelial cells after being submitted to an in vitro monolayer culture system, which is known to lead to cell dedifferentiation, yet, these results provide evidence of a relationship between epigenetic reprogramming and energy metabolism under these cell culture conditions. In conclusion, the levels of metabolites in culture media may be crucial for cellular function and differentiation, meaning that it should be considered in studies culturing oviductal cells.

Effects of Glucose Levels on Inflammation and Amino Acid Utilization in Lipopolysaccharide-Induced Bovine Mammary Epithelial Cells.

Glucose and amino acids are important sources of nutrients in the synthetic milk of dairy cows, and understanding the fate of amino acids is essential to optimize the utilization of amino acids in milk protein synthesis, thereby reducing nutrient inefficiencies during lactation. The purpose of this study was to investigate the effects of LPS and different concentrations of glucose on (1) the expression of inflammatory factors and genes, (2) the glucose metabolism, and (3) amino acid utilization in BMECs. The results showed that there was an interaction (LPS × glucose, p < 0.05) between LPS and glucose content in the inflammatory cytokine genes (IL-6 and TNF-α) and the inflammatory regulatory genes (CXCL2, CXCL8, and CCL5). With the addition of LPS, the HG + LPS group caused downregulated (p < 0.05) expression of IL-6 and TNF-α, compared with the LG + LPS group. Interestingly, compared with the LG + LPS group, the HG + LPS group upregulated (p < 0.05) the expression of CXCL2, CXCL8, and CCL5. LPS supplementation increased (p = 0.056) the consumption of glucose and GLUT1 gene expression (p < 0.05) and tended to increase (p = 0.084) the LDHA gene expression of BMECs under conditions of different concentrations of glucose culture. High glucose content increased (p < 0.001) the consumption of glucose and enhanced (p < 0.05) the GLUT1, HK1, HK2, and LDHA gene expression of BMECs with or without LPS incubation, and there was an interaction (LPS × glucose, p < 0.05) between LPS and glucose concentrations in GLUT1 gene expression. In this study, LPS enhanced (p < 0.05) the consumption of amino acids such as tryptophan, leucine, isoleucine, methionine, valine, histidine, and glutamate, while high levels of glucose decreased (p < 0.01) consumption, except in the case of tyrosine. For histidine, leucine, isoleucine, and valine consumption, there was an interaction (LPS × glucose, p < 0.05) between LPS and glucose levels. Overall, these findings suggest that relatively high glucose concentrations may lessen the LPS-induced BMEC inflammatory response and reduce amino acid consumption, while low glucose concentrations may increase the demand for most amino acids through proinflammatory responses.

Enhanced biosurfactant production by Bacillus subtilis SPB1 using developed fed-batch fermentation: effects of glucose levels and feeding systems.

Biosurfactants stand for highly useful and promising compounds. They basically serve for a variety of applications in multiple industries and aspects of human life. Therefore, it is highly required to improve their production yield especially through the development of new and more efficient fermentation processes. In this aim, batch and fed-batch were studied and compared in terms of their effective biosurfactant production by Bacillus subtilis SPB1. Experiments of fed-batch fermentations were carried out through three different glucose feeding strategies, namely the pulsed, the constant Donespeed and the exponential feeding. The comparison between different fermentation processes revealed that fed-batch process proved to be a more efficient cultivation strategy than the batch process in terms of cell biomass, biosurfactant production and productivity. Among the three different feeding strategies, the exponential feeding process achieved the highest fermentation results of final biosurfactant concentration. The latter increased more than twofolds compared to batch fermentation.

Evaluation of the Regression Coefficient between the Activity of FoxP3 Gene on Regulatory T Cells and Glucose Level in Patients with type 1 Diabetes Mellitus In the City of Mosul

Still, external insulin therapy with its various types causes a variety of immune reactions through its variable control of the glucose level in patients with type 1 diabetes. However, major immune problems are uncommon, and less serious diabetic events have long-term effects on immune responses. The current study aimed to follow up the effect of glucose levels on the level of gene expression of the FoxP3 gene, one of the most important genes responsible for the activity of Regulatory T cells in patients with type 1 diabetes. (26) blood samples were collected from individuals diagnosed with type 1 diabetes for a period of more than (5) years, their ages ranged from (15-35) years, and from both sexes, from Al-Wafa Center for the treatment of diabetics and endocrine glands in the city of Mosul. The patients were divided into four groups, depending on the type of insulin injection , These groups included (Actrapid patients group, Insulatard patients group, Insulatard / Actrapid patients patients group, and finally Mixtard patients group). The control group also included (7) blood samples of healthy people as donors, ages ranging from (15-30) years, (males and females), without communicable diseases, bacterial infections, or autoimmune diseases. . The results showed an effect of glucose concentration on the expression level of the FoxP3 gene by (26%) in the group of patients who used insulin mixtard type, while the results of the effect analysis of glucose on the level of gene expression of the FoxP3 gene showed a rate of (76%) in the group of patients who used insulin type Insulatard, On the contrary, there was no effect of glucose concentration on the level of FoxP3 gene expression in patients using Actrapid and Insulatard / Actrapid insulin at (P≤0.05).

Effect of Glucose Levels on Cardiovascular Risk.

Cardiovascular diseases remain the leading cause of death and disability. The development of cardiovascular diseases is traditionally associated with various risk factors, most of which are somehow related to an unhealthy lifestyle (smoking, obesity, lack of physical activity, etc.). There are also risk factors associated with genetic predisposition, as well as the presence of concomitant diseases, especially chronic ones. One of the most striking examples is, of course, type 2 diabetes. This metabolic disorder is associated with impaired carbohydrate metabolism. The main clinical manifestation of type 2 diabetes is elevated blood glucose levels. The link between diabetes and CVD is well known, so it is logical to assume that elevated glucose levels may be important, to some extent, in the context of heart and vascular disease. In this review, we tried to summarize data on the possible role of blood glucose as a risk factor for the development of CVD.

The Effect of Hyperlipidemia on the Course of Diabetic Retinopathy-Literature Review.

Diabetes mellitus is a very important social issue, and its retinal complications continue to be one of the major causes of blindness worldwide. The effect of glucose level on the development of retinal retinopathy has been the subject of numerous studies and is well understood. Hypertension and hyperlipidemia have been known to be important risk factors in the development of diabetes complications. However, the mechanisms of this effect have not been fully explained and raise a good deal of controversy. The latest research results suggest that some lipoproteins are closely correlated with the incidence of diabetic retinopathy and that by exerting an impact on their level the disease course can be modulated. Moreover, pharmacotherapy which reduces the level of lipids, particularly by means of statins and fibrate, has been shown to alleviate diabetic retinopathy. Therefore, we have decided to review the latest literature on diabetic retinopathy with respect to the impact of hyperlipidemia and possible preventive measures

Effect of glucose levels on carbon flow rate, antioxidant status, and enzyme activity of yeast during fermentation.

The physiological metabolism of yeast has a significant impact on the quality of fermentation products. The present study aimed to investigate yeast metabolism in response to a changing glucose content environment, especially in fermentation products, as well as the change of carbon flow rate, antioxidant status, and yeast enzyme activity. Yeast in a 0g L-1 glucose level was subjected to carbon starvation stress, cell growth retardation and cell proliferation was significantly inadequate; in the logarithmic growth stage of yeast, at a 30 g L-1 glucose level, the carbon source mainly flowed to tricarboxylic acid cycle and pentose phosphate metabolism, cell division, proliferation, and increased cell growth. In later logarithmic growth period and stable period, carbon flowed into glycerol and trehalose metabolism, to cope with the environmental stress; yeast in 60 and 150 g L-1 glucose levels faced high glucose stress at the beginning, the content of reactive oxygen increased, malondialdehyde content increased, cell damage was reduced through the regulation of superoxide dismutase and catalase enzyme activities, and most of the carbon flowed into the metabolic pathway of ethanol, glycerol, and trehalose to cope with high glucose stress, the pentose phosphate pathway showed a large late influx, and NADPH also started to increase rapidly after 24 h. Yeast was stressed in a high-sugar environment and ensured the activity of yeast by preferentially increasing the metabolic intensity of trehalose, glycerol, and glycolytic metabolism, weakening tricarboxylic acid metabolism, and first weakening and then increasing pentose phosphate metabolism. © 2022 Society of Chemical Industry.

Effect of glucose levels on the rheo‐fermentation properties of dough during fermentation

SummaryIn this study, recombinant dough and simulated dough medium were used to study the effect of different glucose levels on quality of dough. With the increase of glucose levels (3, 6, 9, 12 and 15 g/100 g mixed flour) in recombinant dough, the water absorption and extensibility of the dough decreased significantly. Compared with the sugar‐free medium, the maximum height of the dough added with glucose gradually increased, and the CO2 retention rate decreased slightly. The glucose levels in the dough changed the water mobility and distribution. Compared with 3% glucose levels, yeast produced much more glycerol content and ethanol content in 6% and 15% glucose medium, which affected rheological properties of dough. Different glucose levels affected the fermentation state of yeast and metabolites significantly, 3% glucose medium was more suitable for the dough fermentation and growth of yeast during dough fermentation.

Abstract #1037562: Effect of Glucose Level in the Frequency of Discordance Apo B-Low Density Lipoproteins (LDL)

Background: Apo B determination lets to know the number of atherogenic particles and is a risk factor, probably as crucial as LDL determination. However, the effect of glucose level in the frequency of discordant results, low or average LDL with high Apo B, is still unknown in our city

Effect of glucose concentration and culture substrate on HUVECs viability in in vitro cultures: A literature review and own results

Some clinical studies reported that glucose variability increased the risk of developing diabetes-related late complications more than constant hyperglycemia, while others claimed that the evidence was not strong enough to support such a conclusion. A few in vitro studies investigated the effect of constantly high or variable glucose levels (VGLs) on endothelial cells (EC). The first aim of this work was to review these studies and demonstrate that most of them support the notion that viability and other metabolic parameters of EC deteriorate faster in cell cultures with VGLs than in cultures with stable normal or high glucose concentration. The second aim was to verify whether the effect of glucose concentration is the same regardless of other culture conditions such as the substrate on which the cells are grown. We cultured Human Umbilical Vein Endothelial Cells (HUVECs) for 7 or 14 days in constant (5 mM or 20 mM) or variable (switching between 5 mM and 20 mM once a day) glucose concentration in culture plates, which were either not-covered with any additional substrate or were covered with fibronectin or gelatin. We assessed the cell viability using a propidium iodide test. The ANOVA revealed that HUVECs viability was affected not only by glucose concentration and duration of the cell culturing but also by the type of substrate and interactions of these three factors. In conclusion, the effect of glucose level on EC viability should not be analyzed in isolation from other culture conditions that may amplify or attenuate this effect.

The effect of 75-g oral glucose tolerance test on maternal and foetal Doppler parameters in healthy pregnancies: a cross-sectional observational study

Hyperglycaemia can alter placental resistance to blood flow and hyperglycaemia has adverse perinatal outcomes. Oral glucose tolerance testing (OGTT) increases the maternal plasma glucose levels temporarily and mimics metabolic hyperglycaemia. The blood flow of the uterine artery (UtA), umbilical artery (UA), middle cerebral artery (MCA) were assessed before, 1 and 2 h following the OGTT by using Doppler ultrasonography. Z-score of cerebroplacental ratio (CPR), pulsatility index (PI) for three vessels were evaluated separately. All measurements of the MCA, UA, UtA Doppler parameters were not statistically different for fasting, and 1 and 2 h following the 75 g OGTT in the 53 pregnant women with a singleton gestation in the low-risk group. This study results show that acute hyperglycaemia induced by OGTT has no effect on maternal and foetal Doppler parameters in healthy pregnancies. IMPACT STATEMENT What is already known on this subject? Foetal glucose is affected by maternal blood glucose concentrations and placental blood flow. Acute hyperglycaemia may have an effect on maternal, and foetal Doppler parameters among healthy pregnancies What do the results of this study add? Our findings indicate that blood flow velocity metric measurements in the UA, MCA and UtA were not affected by the OGTT in healthy pregnant women. What are the implications of these findings for clinical practice and/or further research? Acute hyperglycaemia induced by OGTT does not have any effect on fetomaternal circulation, especially foetal brain blood flow. Other foetal vessels including ductus venosus, renal artery, etc. may be affected by maternal blood glucose levels during the OGTT or in diabetic patients. Future prospective studies consisting of diabetic patients are warranted to verify the exact effect of glucose levels on foetal and maternal circulation.

Evaluation of antioxidants capacity of non-enzymatic antioxidants and its effect in glucose level in diabetic patients

Background: Oxidative stress might participate in the pathophysiology of diabetes type 2. Systems of nonenzymatic antioxidants are made up of scavenging molecules that are formed endogenously.The Study Objective: This study’s aims were evaluation of the non-enzymatic antioxidants and their effectin levels of glucose among diabetic patients.Methodology: A total of eighty patients with DM type 2 and forty healthy persons (control) were enrolledwithin this study. Serum and plasma were obtained from collected blood samples. The Ferric ReducingAbility of Plasma (FRAP) method was used for detection of the total antioxidant capacity (TAC). Ellman’smethod was used to determine the level of reduced glutathione. The serum CAT activity was detected by thetechnique explained by Sinha.Results: The findings revealed that, among diabetic groups, there was a significant elevation in glycosylatedhemoglobin and glucose levels; HbA1c and FBG levels were significantly higher; MDA, NO, LPI, TAC andGSH showed significant greater values. The activity of SOD showed significantly greater mean value amongthe control group.Conclusion: The results suggest that antioxidants defense might be decreased in T2DM, as TAC levelswere decreased. The increased levels of MDA, NO, LPI, TAC and GSH were associated with oxidativestress. Assessment of GSH could help in recognition of the extent of oxidative stress in diabetes as well asprevention and control of diabetic complications.

The Effect of Hyperglycemia on Infarct Growth after Reperfusion: An Analysis of the DEFUSE 3 trial

Background and purposeBrain infarct growth, despite successful reperfusion, decreases the likelihood of good functional outcome after ischemic stroke. In patients undergoing reperfusion, admission glucose is associated with poor outcome but the effect of glucose level on infarct growth is not well studied. Materials and MethodsThis is a secondary analysis of the DEFUSE 3 trial. The primary predictor was baseline glucose level and the primary outcome is the change of the ischemic core volume from the baseline to 24-hour follow-up imaging (∆core), transformed as a cube root to reduce right skew. We included DEFUSE 3 patients who were randomized to endovascular therapy, had perfusion imaging data at baseline, an MRI at 24 hours, and who achieved TICI 2b or 3. Linear regression models, both unadjusted and adjusted, were fit to the primary outcome and all models included the baseline core volume as a covariate to normalize ∆core. ResultsWe identified 62 patients who met our inclusion criteria. The mean age was 68.1±13.1 (years), 48.4% (30/62) were men, and the median (IQR) cube root of ∆core was 2.8 (2.0-3.8) mL. There was an association between baseline glucose level and normalized ∆core in unadjusted analysis (beta coefficient 0.010, p = 0.01) and after adjusting for potential confounders (beta coefficient 0.008, p = 0.03). ConclusionIn acute ischemic stroke patients with large vessel occlusion undergoing successful endovascular reperfusion, baseline hyperglycemia is associated with infarction growth. Further study is needed to establish potential neuroprotective benefits of aggressive glycemic control prior to and after reperfusion.

Comparative study among Corn silk, Pumpkin Seeds, and Creatine to test the efficiency of physical activity in rat

The present study was conducted to investigate the effect of corn silk,pumpkin seeds and creatine supplementation on kidney function, liver function, glucose, creatine kinase and lactate dehydrogenase in adult male rats subjeced to exercise training. Fifty male albino rats, were fed on basal diet for one week for adaptation. During the adaptive week all rats (except negative control group) were exposed to day after day forced treadmill running exercise for 10 min. Rats were divided into two main groups, the first main group (n=10 rats) was fed on basal diet only and served as negative control group. The second main group (exercised rats) were divided into 4 subgroups (10 rats each) as follows:-Subgroup 1: were fed on basal diet and had run for 10, 15, 20 and 25minutes(15 days for each time periodrespectively) to complete 60 days, and served as positive control group. And the rest of Subgroups (2-4) were fed on basal diet supplemented with 4% corn silk powder, 4% pumpkin seeds powder and 4% creatine powder, respectively as the same exercise of positive control subgroup. At the end of the experimental period (60 day), each subgroup were exposed to the exhausting treadmill test and the run time was recorded, then all experimental rats were scarified.From the obtained results we concluded that pumpkin seed have bioactive compounds that elevate the exercise performance ,and prolong the exhaustive time ,corn silk have a good effect in glucose level by increasing insulin level and recovering injured beta cells, and not via increasing glycogen or inhibiting gluconeogenesis ,although creatine recorded the largest glycogen quantity in gastrocnemius muscles among supplemented groups, but it caused an elevation of ammonia,AST ,and uric acid levels when exposed to exhaustive time test . So future studies are recommended to clarify creatine benefit in exercise field .The study advised to consume corn silk and pumpkin seed to prolong exercise time and accelerate recovery from exercise training.

Evaluation ofAgave lechuguillaby‐product crude extract as a feed additive for juvenile shrimpLitopenaeus vannamei

Agave lechuguilla is a succulent plant species, mainly distributed in the northeast of Mexico and south of the United States of America. The main use for this plant is the fibre´s extraction (known as Tampico fibres), resulting in 15% of fibres and 85% of a by-product waste named guishe. The lechuguilla collectors, normally incinerate the guishe, thus causing environmental contamination. Interestingly, recent studies showed that guishe contains molecules with nutritional properties, such as saponins, flavonoids and sugars. Therefore, in this work, we evaluated the effect of the crude extract of guishe as a feed additive in whiteleg shrimp diets. According to that, MS-HPLC analysis of the extract showed the presence of saponins such as diosgenin, smilagenin, hecogenin, manogenin, tigogenin hexose, yucagenin, chlorogenin, diosgenin diglucoside and the flavonol, quercetin. After chemical analysis, the crude extract was included into an experimental diet in four levels; 0% (L0%), 0.1% (L0.1%), 0.3% (L0.3%) and 0.6% (L0.6%). Dietary incorporation of the extract was evaluated by zootechnical performance, haemolymph biochemistry, histomorphology and digestive enzyme activity of shrimps. After 5-week feeding, the L0.3% diet showed significantly higher growth and better feed utilization among treatments. A significant increase in tubule epithelium height and tubule coverage area from hepatopancreas in shrimp under L0.3% diet compared with the control diet suggest an improvement of the health and nutritional status of the shrimp. Inclusion of L0.3% and L0.6% of the crude extract resulted in a reduction in amylase activity, without effect in glucose levels in the haemolymph. Thus, we suggest that lechuguilla guishe crude extract contains nutritional molecules that may be used as a feed additive to promote shrimp productivity.

Effect of glucose level on chemical hypoxia- and hydrogen peroxide-induced chemokine expression in human glioblastoma cell lines

Glioblastoma multiforme (GBM) is the most common primary intracranial tumor in adults and has poor prognosis. The GBM-specific tumor microenvironment (TME) plays a crucial role in tumor progression, immune escape, local invasion, and metastasis of GBM. Here, we demonstrate that hypoxia, reactive oxygen species (ROS), and differential concentration of glucose influence the expression of cytokines and chemokines, such as IL-6, IL-8, and IP-10, in human glial cell lines. Treatment with cobalt chloride (CoCl2) and hydrogen peroxide (H2O2) significantly increased the expression levels of IL-6, IL-8, and IP-10 in a dose-dependent manner in CRT-MG and U251-MG astroglioma cells, but not in microglia cells. However, we found strikingly different patterns of expression of cytokines and chemokines between H2O2-treated CRT-MG cells cultured in low- and high-glucose medium. These results suggest that astroglioma and microglia cells exhibit distinct patterns of cytokine and chemokine expression in response to CoCl2 and H2O2 treatment, and different concentrations of glucose influence this expression under either hypoxic or oxidant-enriched conditions.

Hyperglycemia Enhances the Production of Amyloid β1-42 in the Lenses of Otsuka Long-Evans Tokushima Fatty Rats, a Model of Human Type 2 Diabetes.

It has been reported that the accumulation of amyloid β1-42 (Aβ1-42) in human lenses can cause some forms of lens opacification. However, the factors leading to changes in the accumulation of Aβ in the lens remain obscure. In this study, we investigate the effect of hyperglycemia on Aβ1-42 accumulation in lenses. Otsuka Long-Evans Tokushima Fatty (OLETF) rats and the human lens epithelial cell line SRA 01/04 (HLE cells) were used. The expression of mRNA was determined using a quantitative real-time RT-PCR method; Aβ1-42 levels were analyzed by an ELISA method. Otsuka Long-Evans Tokushima Fatty rats at more than 20 weeks of age develop diabetes mellitus with hyperglycemia. Additionally, the levels of the mRNAs for Aβ1-42, amyloid precursor proteins (APP), β-(BACE1), and·γ-secretase (PS) rise in the lenses of OLETF rats with age; high Aβ1-42 levels are observed in the lens capsule-epithelium and cortex. The enhanced expression of the genes for APP, BACE1, and PS in the lenses of OLETF rats is prevented by food restriction (25 g/d/rat). When the effect of glucose levels on the production of Aβ1-42 was investigated in the human lens epithelial cell line SRA 01/04 (HLE cells), the mRNA levels for APP, BACE1, and PS, as well as Aβ1-42 protein levels, were significantly higher under high glucose conditions (20 mM) than under normal glucose conditions (5.6 mM). High glucose leads to the increased expression of genes related to Aβ production, resulting in the accumulation of Aβ in the lens.

The effect of glucose level in blood on microbiocenosis of intestine and quality of life of people with predisposition to diabetes mellitus type II.

The article presents analysis of results of examination of spectrum, rate of occurrence and number of microorganisms of large intestine in individuals with normal level of glucose in blood and with predisposition to diabetes mellitus type II. The psychodiagnostic study was carried out including estimate of particular psycho-physiologic and social psychological characteristics of personality reflecting characteristics of psychic and social development. It is established that in individuals with predisposition to diabetes mellitus type II microbiota of intestine altered sideway to dysbiosis degree I-II at the expense of increasing of number of opportunistic microflora up to several degrees. In control group normobiocenosis and intestine dysbiosis degree I are established. It is demonstrated that satisfaction with life-quality is approximately the same in both groups of study participants. However, adaptation possibilities are significantly higher in individuals with predisposition to diabetes mellitus type II.

Effect of glucose levels on high versus low molecular weight hyaluronan and transfection of bone marrow derived human Mesenchymal Stromal Cells

Effect of glucose levels on high versus low molecular weight hyaluronan and transfection of bone marrow derived human Mesenchymal Stromal Cells

Induction of in vitro ketosis condition and suppression using methylsulfonylmethane by altering ANGPTL3 expression through STAT5b signaling mechanism

Ketosis is a condition where ketone bodies are used as the energy source instead of glucose. The state of ketosis becomes dangerous if the fat metabolism in the body is high. When the glucose availability gets limited, liver activates fat metabolism to make glucose for energy. As the results of these, ketone bodies will form resulting the state of ketosis. In the present study, we analyzed the in vitro ketosis condition and the effect of glucose level in ketosis induction. The natural organic sulfur containing compound methylsulfonylmethane (MSM) was analyzed for anti-ketosis activity in normal mouse hepatocyte, FL83B cell model system. Ketosis condition was induced by modulating glucose concentration. Growth hormone receptor (GHR) has a great role in the ketosis condition. We hypothesize that MSM has the ability to regulate GHR signaling through signal transducer and activator of transcription 5b (STAT5b). The whole cell lysate analysis and DNA binding activity analysis also suggested the critical role of STAT5b in ketosis condition. Angiopoietin-like 3 (ANGPTL3) protein is a molecular target to treat ketosis. STAT5b is a transcription factor for ANGPTL3. We elucidated the treatment of ketosis through the control of molecular targets such as ANGPTL3, STAT5b, and GHR by MSM, and we also evaluated the relation between ANGPTL3 and STAT5b signaling molecules as critical regulators.