Effects Of Dietary Probiotics Research Articles

Effects of dietary probiotics and probiotics combined with enzymes on growth performance of rainbow trout (Oncorhynchus mykiss)

Effects of dietary probiotics and probiotics combined with enzymes on growth performance of rainbow trout (Oncorhynchus mykiss)

Effects of Dietary Probiotics (Lactobacillus Acidophilus) on Hematology, Blood Biochemistry and Blood Lipid in Rats with Carbon Tetrachloride Toxicity

Effects of Dietary Probiotics (Lactobacillus Acidophilus) on Hematology, Blood Biochemistry and Blood Lipid in Rats with Carbon Tetrachloride Toxicity

Effect of Dietary Probiotics (Lactobacillus acidophilus) on the Growth Performance of Labeo Rohita

Effect of Dietary Probiotics (Lactobacillus acidophilus) on the Growth Performance of Labeo Rohita

101. Effect of dietary probiotics on the growth performance of Cobb 500 broiler chickens

101. Effect of dietary probiotics on the growth performance of Cobb 500 broiler chickens

Effect of dietary probiotics and prebiotics on the performance of broiler chickens

Effect of dietary probiotics and prebiotics on the performance of broiler chickens

Effect of dietary probiotics on colon length in an inflammatory bowel disease–induced murine model: A meta-analysis

We investigated the effect of probiotic supplementation on inflammatory bowel disease (IBD) by meta-analysis. We included 30 studies to assess the effect of probiotic administration. We estimated the effect size using standardized mean difference, and we evaluated the statistical heterogeneity of the effect size using Cochran's Q test, followed by meta-ANOVA and meta-regression analysis to explain the heterogeneity of the effect size using a mixed-effects model. We conducted Egger's linear regression test to evaluate publication bias. Among the factors evaluated, colon length and myeloperoxidase showed the greatest Q statistic and I2 index, respectively. Colon length, transforming growth factor-β, IL-10, superoxide dismutase, and glutathione showed positive effect sizes in the fixed- and random-effects models. The others (spleen weight, tumor necrosis factor α, IL-1β, IL-6, IL-12, IL-17, IFN-γ, disease activity index, thiobarbituric acid reactive substances, nitric oxide, myeloperoxidase, malondialdehyde, histological score, and macroscopic inflammatory score) showed negative effect sizes in the fixed- and random-effects models. Probiotics showed a significant effect on all investigated factors, except IL-10. In meta-ANOVA and meta-regression analysis, Lactobacillus paracasei was the most effective probiotic for colon length. Lactobacillus paracasei, Lactobacillus reuteri, Lactobacillus fermentum, and a mixture of Lactobacillus bulgaricus and Saccharomyces boulardii (LC + SB) were effective for colon length, tumor necrosis factor α, IL-6, IL-10, IFN-γ, and disease activity index. Lactobacillus rhamnosus was most effective for IL-10 and IFN-γ. Dietary probiotics are effective in improving the symptoms of IBD. Although the results of this meta-analysis had some limitations due to a lack of animal experiments, they will be meaningful to people with IBD.

Effect of dietary probiotics and prebiotics on the performance of broiler chickens

The prolonged use of antibiotics has led to the development of resistant bacteria and also led to accumulation of antibiotic residue in the poultry feed, this ultimately led to the prohibition of antibiotics as growth enhancers in animal production. Thus, there was a dire need for alternate sources to help in poultry production. Recently, probiotics and prebiotics claimed to be effective alternatives to antibiotics in the poultry. The objective of this study is to investigate the effect of different probiotics and prebiotics on the performance of broilers. The study involved 2 broiler cycles, 1 during winter and 1 during summer with a total of 425 1-day-old Cobb 500 broiler chicks for each cycle. They were allotted to 5 experimental treatments. The probiotics were Bacillus coagulans (1g/kg dried culture) and Lactobacillus (1g/kg dried culture of 12 commercial strains). The prebiotics included fructo-oligosaccharides (FOS) (5g/kg) and mannan-oligosaccharide (MOS) derived from Saccharomyces cerevisiae (5g/kg). The results showed that there was no effect of the different probiotics and prebiotics on the production performance of broilers. There was increased weight of the thymus in the control group. In cycle 1, the panelists indicated that the smell, color, taste, and texture of the cooked meat were acceptable, and that there were no significant differences between the different groups. There was no significant effect of the different diets on the biochemical parameters of the blood among the experimental groups at 3- and 5-wk of age. Phytohaemaglutinin test showed that dietary FOS and MOS induced higher cellular response than the other treatments (P = 0.04) in the first cycle. In the second cycle, the results revealed that dietary FOS induced higher cellular response than the other treatments (P = 0.019). The used experimental treatments have a positive effect on microbial count in 5-week-old broilers. There was no Salmonella sp. recorded using the experimental treatments in the first cycle, and the growth of E. coli was reduced significantly. In the second cycle, all treatments in 3-week-old broilers did not affect the count of both lactic acid bacteria (LAB) and E. coli. At 5-week-old of the same cycle, the bacterial count of E. coli increased even with control, whereas Salmonella growth was inhibited. The pH value was driven toward acidity in all of the treatments. Probiotics and prebiotics can be used in chicken feed safely and without any adverse effects on the productive parameters and immune status of the flock.

Effects of Bacillus subtilis and coccidial vaccination on cecal microbial diversity and composition of Eimeria-challenged male broilers.

In a companion study, the effects of dietary antibiotic alternative and coccidial vaccination on the growth performance of male broilers have been reported. In this paper, the effects of dietary probiotics and coccidial vaccination on diversity and composition of cecal microbiota were investigated using a 3 (diets) × 2 (vaccinated or non-vaccinated) factorial setting of treatments. Three diets, including a corn and soybean-meal control diet, an antibiotic diet (a control diet supplemented with bacitracin and salinomycin), and a probiotic diet (a control diet supplemented with Bacillus subtilis) were provided to broiler chicken from day 0 to 42. To simulate an Eimeria challenge in the field, all chicks were gavaged with a 20× dose of commercial coccidial vaccine containing live Eimeria oocysts on day 14. Cecal contents were collected on day 42. High-throughput sequencing of the 16S rRNA gene was used to determine microbial diversity and composition. Coccidial vaccination to broilers reduced bacterial diversity (Shannon index) of the cecal microbiota. There was a significant interaction between the dietary additive and coccidial vaccination on the observed bacterial species number. Diets supplemented with B. subtilis increased bacterial species of non-vaccinated broilers but decreased bacterial species of vaccinated broilers. In contrast, diets supplemented with antibiotics reduced bacterial species of broilers from both groups. Interactions between dietary additive and coccidial vaccination were also observed on microbial composition. Vaccinated broilers fed the B. subtilis diet exhibited the lowest Firmicutes percentage and highest Bacteroidetes percentage within the microbial community. In addition, vaccinated broilers fed the B. subtilis diet exhibited the highest Rikenella microfusus percentage. From this study, the coccidial vaccination on the day of hatch reduced the microbial diversity of broilers at a later age. The inclusion of B. subtilis-probiotics in the feed of vaccinated broilers may reduce microbial diversity in cecal content by increasing the proportion of a predominant bacterial species, R. microfusus, in the microbial community.

The Effect of Dietary Probiotics on Growth, Survival and Stress Resistance of Rainbow Trout (Onchorynchus Mykiss Walbaum)

The Effect of Dietary Probiotics on Growth, Survival and Stress Resistance of Rainbow Trout (Onchorynchus Mykiss Walbaum)

Effect of dietary probiotics and stocking density on carcass traits, meat quality, microbial populations and ileal histomorphology in broilers under hot-climate conditions

The objective of the present study was to investigate the potential effects associated with dietary probiotic inclusion and the stocking density on carcass traits, meat chemical composition, meat sensory quality, microbial populations and ileal histomorphology in broiler chickens raised under hot climate conditions. In total, 1800 1-day-old unsexed broiler chicks (Ross 308) were randomly allocated in a completely randomised design according to a 3 × 2 factorial arrangement, with three concentrations of a dietary probiotic (0, 200 and 400 mg/kg) containing 4 × 109 cfu/g of Bacillus subtilis and two stocking densities (12 or 18 birds/m2), forming six treatments, with three pens (replicates) each. The probiotic concentration had no significant (P > 0.05) effect on bodyweight gain, feed consumption, feed conversion ratio, carcass percentage and meat chemical composition. Dietary probiotic inclusion significantly (P = 0.02) increased the scores of meat colour and odour. The acceptability score was significantly (P < 0.03) affected by the stocking density. Dietary supplementation of the probiotic at both 200 and 400 mg/kg significantly (P = 0.05) reduced the counts of Escherichia coli and Salmonella in the gut and litter. In meat, dietary supplementation of the probiotic at 200 and 400 mg/kg significantly (P = 0.03) reduced the counts of E. coli, compared with those of the control group. Moreover, Salmonella was not detected in meat. Regarding the ileal villi and crypt morphology, dietary probiotic supplementation significantly (P = 0.05) increased the height of the villus. There were no significant probiotic concentration × stocking density interactions for any of the investigated parameters, except for the gizzard percentage. Thus, dietary probiotic supplementation in broilers raised under a high ambient temperature had a significantly positive effect on the ileal villus height and a significantly negative effect on the counts of E. coli and Salmonella in the gut and litter. No negative effects on growth performance, carcass parts and meat quality were detected.

The effects of dietary probiotics and humic acid on meat quality of Japanese quail including sex-related differences and economical background

The effects of humic substances (HS) on the quality traits (colour, water-holding capacity, and pH), serum minerals (calcium, phosphorus, sodium, potassium, chlorides) and on the serum metabolites (glucose, total protein, triglycerides and cholesterols) of breast and thigh meats of Japanese quails were studied. A total of 60 birds (30 males and 30 females) were involved in the experiment. The animals were fed by the standard basal diet and divided to the 6 groups as follows: probiotic females (PF, n = 10) and probiotic males (PM, n = 10) received probiotic preparation in the single dose of 1 g/1 kg of feed mixture, humic acids females (HF, n = 10) and humic acid males (HM, n = 10) received humic acids in the single dose 3 g/1 kg of feed mixture. The group fed basal diet without any additive served as the control group (CF; n = 10, CM, n = 10). After 210 days the animals were slaughtered, blood samples and samples of muscles (breast and thigh) were collected. Probiotic and humic acids treatment resulted in the significant increase of serum calcium levels in the female groups against the male groups. The content of serum phosphorus significantly increased in the PF group in comparison to the PM group. In this study both treatments (probiotics and humic acids) significantly decreased amount of HDL cholesterol in the female groups in comparison to the female control. Significant sex-based differences were observed for HDL cholesterol (CF, PF, HF vs. CM, PM, HM) and LDL cholesterol (HM vs. HF). 24 h after slaughter meat pH showed significant differences in different muscles. Other investigated parameters of Japanese quails were not influenced by the treatment. We can conclude, that the effect of probiotics and humic acids treatment seems to be dose dependent and estimation of effective dose of additives used in poultry feeding play an important role. Further studies are needed.

Effects of dietary probiotics on beef cattle performance and stress

Abstract Dietary probiotics have been shown to benefit cattle production; however, little is known about how they affect beef cattle temperament and stress. Therefore, the objectives of this study were to evaluate how dietary probiotics impact beef cattle performance and stress. Two treatments were used for this study: (1) control, receiving feed without probiotics and (2) probiotics, receiving feed containing 10 g per head per day of probiotics (Probios feed granules, Chr. Hansen Inc., Milwaukee, WI). Three groups of cattle were evaluated. Group 1 consisted of 7 weaned calves that were 7-10 months old and used a crossover experimental design where the calves were given each treatment for 3 weeks. Group 2 consisted of 33 female cattle ≥ 1 year of age and had treatments applied for 6 weeks using a randomized complete block design (control n = 16, probiotics n = 17). Group 3 consisted of calves that were nursing group 2 cows allocated to the control and probiotics treatment for 6 weeks (n = 8 per treatment). Weekly body weight, average daily gain, feed efficiency (group 2 only), chute exit velocity, behavior during a novel object test (group 1 and 2), and serum cortisol during weeks 0 and 6 (group 2) were measured. Group 1 cattle receiving probiotics had a greater overall change in body weight, average daily gain, and gain:feed ( P ≤ 0.05). No differences in group 2 or 3 growth performance were observed ( P > 0.10). No treatment effect was observed for group 1 weekly chute exit velocity; however, group 2 and 3 cattle of the probiotics treatment had an overall slower weekly chute exit speed compared with control cattle ( P ≤ 0.02). No treatment differences were observed in serum cortisol concentrations in group 2 cattle during weeks 0 or 6 ( P ≥ 0.72). During the novel object test, group 1 cattle receiving probiotics exited the arena slower and group 2 cattle receiving probiotics crossed zone lines more frequently and had less frequent vocalizations compared with control treatment cattle ( P ≤ 0.05). These results suggest that dietary probiotics can improve performance in weaned, growing calves and may have benefits for cattle handling without negatively affecting cattle welfare with respect to stress.

Effect of dietary probiotics on the semen traits and antioxidative activity of male broiler breeders

This study aimed to investigate the effect of probiotics on the intestinal morphology, intestinal microflora, oxidative activity (biological antioxidant potential), and semen quality of male broiler breeders. For this, 180 Cobb male broiler breeders (60 weeks of age) were randomly distributed into two groups. The control group was fed a basal diet, and the probiotics group was fed basal diet supplemented with probiotics for 6 weeks. Probiotics containing Bacillus amyloliquefaciens TOA5001 improved the above mentioned characteristics of the male broiler breeders. Thus, B. amyloliquefaciens TOA5001 might improve the reproductive performance of male broiler breeders.

Effect of dietary probiotics on the growth performance, meat quality improvement of broiler chicken for safe meat production

A feeding trial was conducted to investigate the effect of Probiotic (Biofast) on the growth performance and meat quality of broiler chicken. Ninety six 1-d-old mix sexed Cobb 500 broiler chicks were obtained from a local commercial hatchery. Chicks were randomly allocated in 2 experimental treatments for 5 wk. The experimental treatments received a 1) control, 2) control with probiotic Biofast 0.055% (bacillus subtilis-100%) in the diet. Both treatments had 48 broilers arranged in 4 replicates of 12 broilers each. Eight birds from 2 different groups (one/replication) were sacrificed on termination of the feeding trial of breast, thigh to investigate the meat quality. Feed intake in treatment T1 was significantly (P > 0.05) greater than control in 0 to 3 weeks of age. Though in starter phase treatments failed to induce any marked effects on body weight, weight gain and FCR but numerically increased in T1 than control. In finisher period (4-5 wks), there were no significant differences on body weight, weight gain, feed intake and FCR between the treatments. The organ weight like liver, heart, kidney, spleen, gizzard, abdominal fat and intestine weight also did not show any significant differences between the dietary treatments of control and Biofast. In addition, though the dressing % was not significantly different but numerically higher percentage was found by using Biofast. Similarly, no significant differences (p>0.05) were found in pH, cooking loss, meat color and TBA values between the treatments. In conclusion, supplementation of Biofast in diet has no significant effect on the growth performance of broiler chicks though it has got some positive effects on other parameters that indirectly revealed to enhance meat quality of broiler chicken as well as food safety issues.
 Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 51-57

407 Effects of dietary probiotics on cattle growth performance

407 Effects of dietary probiotics on cattle growth performance

395 Effects of dietary probiotics on cattle stress behavior

395 Effects of dietary probiotics on cattle stress behavior

Effects of probiotics dietary supplementation on growth performance, innate immunity and digestive enzymes of silver pomfret, Pampus argenteus

The effects of dietary probiotics on growth performance, innate immunity and digestive enzymes of silver pomfret were investigated. Compared to the control, diets supplemented with Bacillus subtilis, Lactobacillus plantarum and Clostridium butyricum caused significant improvements of growth performance, including final weight, weight gain, specific growth rate and feed conversion rate. A better growth performance among probiotic treatments was obtained in fish fed B. subtilis. To reveal the effects of these three probiotics on innate immunity of silver pomfret, lysozyme activity, SOD activity and IgM concentrations were examined. Results showed that silver pomfret fed diets containing C. butyricum, L. plantarum and B. subtilis had led to an enhanced innate response, notably higher lysozyme activity, SOD activity and IgM concentrations, as recorded after 30 and 60 days of feeding. In addition, study of different digestive enzymes viz lipase, protease and amylase activities showed significant higher values in fish fed the probiotic diets in comparison to the untreated group. These findings demonstrated that administration of C. butyricum, L. plantarum and B. subtilis can improve growth performance through enhanced immune response and digestive enzyme activity in silver pomfret. Furthermore, the B. subtilis is highly recommended as dietary probiotics in silver pomfret.

Biological effects of dietary probiotics on blood characteristics in Hanwoo heifers subjected to lipopolysaccharide (LPS) challenge

This study hypothesized that dietary feed additive containing probiotics alter either immune-related serum substances or serum metabolites in Hanwoo heifers. A probiotic treatment was given at 0.5% top-dressing of concentrate diet for 6 months. The change of immunological indicators in the blood was analyzed under LPS (Lipopolysaccharide) challenge. One day before administration of LPS, all heifers were fitted with an indwelling jugular vein catheter for serial blood collections. Both a serum tube and an EDTA-coated tube were collected at 30-min intervals from - 2 to 8 hours relative to the LPS challenge at time 0 (1 ㎍/㎏ of BW). Serum was used for analyzing albumin (ALB), glucose (GLU), total protein (TP), triglycerides (TG), phosphorus (IP), and non-esterified fatty acids (NEFA). Plasma was used for analyzing white blood cell (WBC), red blood cell (RBC), platelet (PLT) and inflammation-related factors (NE, LY, MO, EO, BA, Hb, HCT, MCV, MCH, MCHC, RDW, MPV). There were significant differences in ALB, GLU, TG, IP, and NEFA concentration with the passage of hours post challenge (p 0.05). The level of WBC, EO, LY, and MO were reduced after LPS challenge (p > 0.05). In conclusion, LPS challenge after dietary supplementation of probiotics changed the levels of both serum metabolites and inflammation-related factors. The increase of GLU and TG indicated a probiotics-positive response under LPS challenge (p < 0.05).

THE EFFECT OF PROBIOTIC DIET ON HEMATOLOGY AND PISCICULTURAL PARAMETERS OF YEARLING HYBRIDS OF RUSSIAN AND SIBERIAN STURGEON

In fish farming practices, stocking density is a key factor in determining the productivity and profitability of commercial fish farms. the use of probiotics as farm animal feed supplements increases the animals growth and improve its health by increasing its resistance to disease. this study was undertaken to examine the effect of dietary probiotics on yearling hybrids of russian and siberian sturgeon, based on hematological profile and piscicultural performance. the experience was carried out in 4 groups (control and treatment). in control group, probiotic was not applied in diet but in treatment groups, probiotics Bacell, Monosporin, and Prolam was administered in feed at a concentration of 2%. as a result, a positive influence on growth performance and survival rate were assessed together with improvement of physiological status (based on hematological indices). thus, probiotics addition to diet had the effect on erythrocyte count, hemoglobin, leucocyte count, percent of lymphocyte, serum total protein, percent of neutrophile and monocyte the results suggest that probiotics can stimulate immune parameters hybrids of russian and siberian sturgeon and can be successfully used in fishfarming.

Effects of Dietary Probiotics on the Growth and Feeding Efficiency of Red Hybrid Tilapia, Oreochromis sp., and Subsequent Resistance to Streptococcus agalactiae

An eight-week trial was performed to evaluate three commercial/prototype probiotics supplemented in red hybrid tilapia, Oreochromis sp. diets. Triplicate groups of tilapia were measured for growth performance, feeding efficiencies, and whole-body composition. After the feeding trial, duplicate groups of tilapia were assessed for their resistance to Streptococcus agalactiae over 23 days. Six diets were supplemented with 0.1% or 0.3% PB1 consisting of Bacillus subtilis, 0.1% or 0.3% PB2 consisting of B. licheniformis or 0.1% MPB consisting of Bacillus sp. and Pediococcus sp. Probiotics had no effect (p > 0.05) on growth or feeding efficiencies, although whole-body crude protein was significantly higher in the PB1 0.3% treatment. Tilapia in the probiotic treatments had a higher resistance to S. agalactiae and, with the exception of the PB2 0.1% diet, were all significantly higher than the control treatment. Although the tested probiotics were not growth promoters, dietary B. subtilis was the most effective prophylactic against pathogenic bacteria.