Effect Of Diet Therapy Research Articles

Оценка иммунного статуса у беременных женщин с отягощенным аллергологическим анамнезом

Most researchers emphasize that in the structure of diseases of infants, there is a predominance of pathological conditions that occur precisely in the perinatal period, which indicate the correlation of these diseases with the health of the mother and the course of her pregnancy. Objective. Assessment of the immune status and effectiveness of diet therapy in pregnant women with burdened allergic history. Patients and methods. The observation groups consisted of 118 pregnant women. The first group included 83 pregnant women who received a hypoallergenic diet with the exclusion of main allergens, with the replacement of cow's milk with goat's milk, or the exclusion of dairy products as a causally significant allergen. The second group consisted of 35 pregnant women who followed the usual dietary recommendations offered in women's consultations, with the inclusion of cow's milk in the diet. The content of total IgE and allergen-specific IgE antibodies to food proteins in blood serum was determined by the immunoblot method using PROTIA Allergy-Q diagnostic panels. Results. 71.1% of pregnant women of the first group and 40% of women of the second group had the history of allergic diseases. According to skin scarification tests, these women had polyvalent sensitization. When studying the immunological status of pregnant women, attention was drawn to the fact that high levels of total serum IgE were in patients with allergies, with diseases of the gastrointestinal tract, as well as with a combination of these pathologies. 37.3% of the observed pregnant women of the first group, both with and without allergies, had high levels of total serum IgE. At the same time, the level of total IgE was higher in pregnant women suffering from allergic diseases. In addition, IgE sensitization to milk proteins was found in women with normal and high levels of total IgE in the blood. The concentration of allergen-specific IgE antibodies to cow's milk proteins was higher in women with IgE-mediated allergies. The use of a hypoallergenic diet in pregnant women caused the most pronounced decrease in the total IgE content in the blood compared with the indicators before treatment. The majority of the observed women showed a positive trend towards a decrease in IgE sensitization to milk proteins against the background of dietary therapy. Conclusion. All pregnant women with allergic diseases had food sensitization. Gastrointestinal pathology was observed in 34.7% of pregnant women, and its frequency in women with clinically confirmed allergies was 2.3 times higher than in women without allergies. A highly sensitive method for diagnosing food sensitization using PROTIA Allergy-Q panels has made it possible to most accurately identify causally significant allergen products, develop and effectively use personalized hypoallergenic diets for pregnant women. Considering the concept of food programming, the use of hypoallergenic diets in the diet of pregnant women with allergic diseases is one of the options for the prevention of allergic diseases in the future in children born to such mothers. Key words: pregnant women, allergy, food IgE sensitization, diet therapy

Современный взгляд на функциональные запоры у детей раннего возраста: особенности вскармливания и принципы немедикаментозной коррекции

Статья посвящена проблеме хронического запора у детей. Даны рекомендации по профилактике этого состояния. Подчеркнуто, что к медикаментозной терапии рекомендуют прибегать только при отсутствии эффекта от диетотерапии, которая сводится не к строгой диете или соблюдению определенного лечебного стола, а к физиологическому рациону питания. В настоящее время доступны смеси, соответствующие рекомендациям Европейского союза относительно питания специального медицинского назначения, сочетающие в себе одновременно несколько функциональных компонентов, что позволяет решить проблему функциональных запоров у детей раннего возраста, находящихся на искусственном вскармливании. Примером такой инновационной смеси является Нутрилон Комфорт.

Basic principles of dietary therapy in type 2 diabetes mellitus: focus on antioxidant protection and endothelial dysfunction

Oxidative stress and endothelial dysfunction have been identified as the most important pathogenetic pathways for the development and progression of vascular complications in type 2 diabetes (T2DM). Objective. To evaluate the effect of individual dietary components on oxidative stress and endothelial dysfunction in patients with T2DM. Material and methods. The search and analysis of publications was carried out using the PubMed, MEDLINE, Web of Science databases, mainly for the last 10 years, using the keywords "type 2 diabetes mellitus", "endothelial dysfunction", "polyphenols", "antioxidants", "diet". Results. It has been shown that despite the progress in the development of drugs for the correction of clinical and metabolic disorders in T2DM, diet therapy is an important therapeutic factor influencing the main mechanisms of development and progression of cardiovascular complications in T2DM, including hyperglycemia, dyslipidemia, arterial hypertension, subclinical inflammation, endothelial dysfunction. Properly organized and built on modern scientific principles, clinical nutrition improves glycemic control, correction of arterial hypertension, dyslipidemia and overweight/obesity. Influencing the mechanisms of development of oxidative stress, therapeutic nutrition can serve as a preventive approach to protect against the occurrence of endothelial dysfunction and subsequent complications, primarily atherosclerotic origin. Conclusion. The review presents current data on the effect of diet therapy on the main clinical and metabolic parameters in T2DM, endothelial function and oxidative stress, as the most significant factors in the development of systemic vascular complications.

Evaluation of the effectiveness of diet therapy in the elderly with obesity and non-alcoholic fatty liver disease

The aim of the study was to evaluate the effectiveness of a standard reduced-calorie diet in elderly subjects with obesity and nonalcoholic fatty liver disease (NAFLD).Materials and Methods: 60 women with I-III degree obesity and NAFLD were examined. Metabolic status was assessed by indirect calorimetry and body composition indices using bioimpedance spectroscopy. Biochemical indices in blood serum were determined on a biochemical analyzer “KONELAB Prime 60i”. SPSS Statistics 23.0 program was used for statistical data processing.Results and Discussion: It was found that resting energy expenditure was significantly higher in middle-aged women (1896.8±46.5 kcal/d) with obesity and NAFLD than in elderly women (1691.6±34.2 kcal/d). Fat oxidation rate was significantly lower in elderly women (66.8±7 g/day) than in middle-aged women (70.8±9 g/day). The elderly patients received a variant of the standard diet with reduced caloric content for 10 days. Against the background of diet therapy, a significant decrease in glucose, total cholesterol, LDL, triglycerides, as well as fat mass, total fluid and visceral fat area was observed in elderly patients with obesity and NAFLD. However, the use of a standard reduced-calorie diet resulted in a slight elevation of serum uric acid levels and a significant decrease in muscle mass.Conclusions: the selection of specialized diets is recommended for elderly patients with obesity and NAFLD when prescribing diet therapy, which will not only prevent the decrease of muscle mass and increase of uric acid but also improve the effectiveness of treatment measures in obesity by preventing the development and progression of its complications.

Innovative food technologies in the treatment of cardiac surgery patients with concomitant obesity

Purpose. The aim of the study was to develop a technology for dietary correction of nutritional and metabolic status disorders in patients with coronary heart disease with concomitant obesity in the system for comprehensive preoperative preparation.Material and Methods. The study was performed in the Department of Cardiovascular Pathology and Diet Therapy of the Federal Research Center for Nutrition, Biotechnology and Food Safety. The study comprised a total of 76 patients with coronary artery disease and obesity who required surgical myocardial revascularization and received the course of specialized targeted diet therapy. Patients were assigned to two groups: control group comprised 16 men and 25 women aged 65.6 ± 1.37 years; main group comprised 12 men and 20 women aged 66.0 ± 1.87 years. Patients of control group received a reduced calorie diet during 30-day treatment. Patients of main group additionally received a specialized food product for therapeutic dietary nutrition developed specifically for this category of patients (modified diet therapy). The effects of diet therapy on the parameters of nutritional and metabolic status were assessed.Results. The study showed that administration of modified diet therapy allowed to achieve more pronounced reduction of body weight and body mass index as well as optimization of body composition, basal metabolic rate, and blood lipid profile including a significantly more pronounced decreases in the levels of total cholesterol (p < 0.001), triglycerides (p < 0.05), low density lipoprotein cholesterol (p < 0.01), and atherogenic coefficient (p < 0.01).Conclusion. The proposed technology of nutritional care for patients with coronary artery disease and obesity may be used for the purpose of preoperative preparation for surgical myocardial revascularization.

Сучасні погляди на функціональні запори у немовлят

У даній статті йдеться про те, що запор є поширеною проблемою та спостерігається приблизно у 3–14 % немовлят, що звичайно турбує батьків і спонукає звертатись за медичною допомогою. Функціональний запор часто спостерігається у немовлят, особливо при переході з грудного молока на штучне вигодовування або на тверду їжу. При цьому чим молодша дитина, тим вище ймовірність анатомічних або органічних причин, хоча частота функціонального запору досягає 97 % усіх випадків запору в дітей. У статті обговорюється доцільність та ефективність дієтотерапії і медикаментозного лікування функціонального запору в немовлят.

Considerations for continuing diet therapy in patients with epilepsy during the COVID-19 pandemic: A scoping review.

Diet therapy is an option for managing patients with drug-resistant epilepsy (DRE). Diet therapies used for patients with epilepsy include the classic ketogenic diet, the modified Atkins diet, the medium chain triglyceride diet, and the low glycemic index diet. The effectiveness of diet therapy is highly dependent on dietary adherence. Coronavirus disease (COVID-19), a novel infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has substantially impacted the lifestyles of people worldwide. Therefore, continuing a specific diet during the COVID-19 pandemic is expected to be difficult in some aspects. Here, based on the available literature, we summarize the perspectives for maintaining diet therapy during the COVID-19 pandemic, as a scoping review. Epileptologists and dieticians need to understand the difficulty of maintaining diet therapy during the COVID-19 pandemic. In addition, the education of patients and all stakeholders to continue diet therapy even during the current COVID-19 pandemic is crucial to control epileptic seizures. In conclusion, continuing diet therapy is not a matter only for patients during the COVID-19 pandemic, but medical staff should also help patients to overcome the problems associated with the pandemic by understanding their situations.

The effect of diet therapy on the anthropometric and metabolic parameters in women with infertility and obesity

Aim: to monitor the anthropometric, hormonal and metabolic parameters in women of reproductive age with infertility and grade I gluteofemoral obesity during treatment with a balanced hypocaloric diet. Materials and methods . The study included 23 infertile women of reproductive age. All patients were prescribed a balanced, low-calorie diet (1200 kcal/day), exercise, and behavioral therapy. The anthropometric, metabolic and hormonal parameters were determined before and 12 months after the treatment. We measured the levels of leptin, total cholesterol, triglycerides, triglycerides, high-density and low-density lipoprotein cholesterol (HDL cholesterol and LDL cholesterol), fasting glycose. Following the measurements, the atherogenic and insulin resistance indices were calculated. In addition, the plasma concentrations of luteinizing hormone, follicle-stimulating hormone, prolactin, estrone, estradiol, testosterone, progesterone, and immunoreactive insulin were measured. Results. Twenty patients completed the study. A decrease in body weight (by average of 10.58 ± 5.70 kg) was found in 87 % of patients; in parallel, the body mass index decreased from 32.53 ± 1.40 to 28.55 ± 2.50 kg/m2 (p < 0.001). The decrease in body weight was accompanied by a statistically significant decrease in the levels of leptin (p < 0.001), estrone (p = 0.049), estradiol (p = 0.032), total cholesterol (p < 0.001), triglycerides (p = 0.004), LDL cholesterol (p = 0.001), fasting glycose (p < 0.001), insulin resistance index (p = 0.002), atherogenic index (p < 0.001) and an increase in the level of progesterone (p = 0.030). The ovulatory menstrual cycle was restored in all women (p = 0.007); within one year, wanted pregnancy occurred in 1 woman. Conclusion. The use of a low-calorie diet for 12 months in infertile women with grade I gluteofemoral obesity leads to normalization of metabolic parameters, weight loss and, the restoration of the ovulatory menstrual cycle in 100 % of cases.

ЕФЕКТИВНІСТЬ ЕЛІМІНАЦІЙНОЇ ДІЄТИ У ДІТЕЙ ГРУДНОГО ВІКУ З ГАСТРОІНТЕСТИНАЛЬНИМИ ПРОЯВАМИ ХАРЧОВОЇ АЛЕРГІЇ

&#x0D; &#x0D; &#x0D; The purpose of the work was to evaluate the effectiveness of the elimination diet in infants with gastrointestinal manifestations of food allergy.Patients and methods. 84 children aged 2 months to 1 year were examined with gastrointestinal food allergy to cow's milk proteins. All children were prescribed an elimination diet with the exclusion of cow's milk proteins. An assessment was made of the regression of gastrointestinal symptoms on day 3, 7 and 14 of diet therapy and the determination of the concentration of eosinophilic cationic protein in serum. The general-clinical, immunological, allergological and statistical methods of research were used.Results. The purpose of the elimination diet with the exclusion of cow's milk proteins for nursing mothers and the use of mixtures with extensive hydrolysis of protein in the case of artificial feeding leads to a complete regression of gastrointestinal and skin symptoms in all examined children on the 14th day of diet therapy, which is further confirmed by the normalization of the concentration of eosinophilic cationic serum protein in diet therapy. Conclusions. Elimination of cow's milk proteins in infants with gastrointestinal food allergy results in complete regression of gastrointestinal and skin symptoms on day 14 of diet therapy, and determination of the concentration of eosinophilic cationic protein in serum can be used as an additional criterion for the effectiveness of diet therapy.&#x0D; &#x0D; &#x0D;

Clinical biochemistry methods in objectiva evalution of overeating foood of carnivores (meat) by a phylogenetically herbivorous homo sapiens (a patient).

The abuse of food of carnivores (meat) by phylogeneticallyI herbivorous Homo sapiens (a patient) initiates atherosclerosis. Addressing biogenetic law of E. Haeckel that ontogeny recapitulates phylogeny (a universal anamnesis), we suggest a diagnostic technique that allows evaluation of the meat diet abuse by a herbivorous Homo sapiens. This technique is based on application of phylogenetic theory of general pathology to clinical practice. The degrees of objective evaluation of nonphysiological overeating of meat are: the first, an increase in the fast plasma content of oleic triglycerides palmitoyl-oleyl-palmitate (POP). The second, hyperglyceridemia + an increase in low density lipoprotein cholesterol (LDL-CL) content. The third, increased plasma content of apoС-III. The fourth, an increase in the concentration of apoВ-48. If electrophoregrams are analyzed and hyperlipoproteinemia (HLP) type is determined according to WHO classification, the first degree of meat overeating is not informative, the second, corresponds to type IV HLP; the third, to type IIb HLP, and the forth, to type V HLP, i.e, the patient diet consists practically of the food of carnivores. Hyperlipoproteinemia coincides with insulin resistance yndrome, hyperglycemia and hyperinsulinemia, which is based on blood increase of fatty acids in the form of polar unesterified fatty acids (UFA). According to phylogenetic theory of general pathology, in vivo cells do not internalize glucose if there is a possibility to internalize UFA. Preventive examination allows evaluation of disorders in the biological function of trophology (food consumption). Thus, the use of different methods in the analysis of this function offers evaluation of the effectiveness of diet therapy from the level of disorders when treatment was started.

Оценка эффективности диетотерапии с включением витаминов-антиоксидантов у пациентов с ожирением и гиперурикемией

Оценка эффективности диетотерапии с включением витаминов-антиоксидантов у пациентов с ожирением и гиперурикемией

Dietary Regulation of miR-33b and miR-29a in Relationship to Metabolic Biomarkers of Glucose and Lipids in Obese Diabetic Women: A Randomized Clinical Controlled Study

Background: MicroRNAs have recently been introduced as epigenetic regulators of glucose and lipid metabolic pathways, which are impaired in obesity and diabetes. Objectives: We evaluated the effects of calorie-restricted diet therapy on the circulating levels of miR-33b and miR-29a in relationship to glucose and lipid metabolic parameters in obese patients with type 2 diabetes mellitus (T2DM). Methods: This randomized clinical controlled trial was performed on 30 eligible obese women with T2DM, randomly divided into two groups (control group, n = 15; diet therapy group, n = 15) for 10 weeks. Ten healthy women with normal weight were enrolled at the baseline of the study as controls. Demographic information, dietary intake, and anthropometric and biochemical indices were obtained before and after the study. Circulating miR-33b and miR-29a were assessed for all subjects using quantitative RT-PCR, and the fold change of each circulating miRNA was compared between groups. Results: The circulating levels of miR-29a and miR-33b in the diabetic women were higher (0.40-fold) and lower (1.43-fold), respectively, than normal levels. Diet therapy significantly increased the circulating level of miR-33b (P = 0.023, 0.97-fold upregulation) to normal levels. This increase was independently correlated with caloric restriction (95%CI: -0.004 to -0.0001, P = 0.022) and 2hPPBS (95%CI: -0.009 to -0.001, P = 0.035). No remarkable change was observed in circulating levels of miR-29a. Conclusions: Our findings introduced a novel therapeutic effect of diet therapy on circulating miRNAs in obese patients with T2DM. MiR-33b is an important therapeutic target in the treatment and prevention of T2DM and its complications

Investigating the Effect of Diet Therapy on Reducing Women’s Overall Self-Esteem

Overweight and obesity make physical and psychological complications for people. In this regard, Low self-esteem will be the most important psychological condition. This study investigates the effect of diet therapy of women (reduction in body mass) on reducing their overall self-esteem. The population of the study was the women who were referred to diet therapy clinic in Isfahan (2014). The number of 30 subjects among them was selected. In order to measure women’s self-esteem, Cooper Smith Self-Esteem Test is used for demographic data, a self-measured questionnaire was used. Analysis of the data in the study was descriptive and inferential statistics. The results show that there is a significant difference between the self-esteem of women in four steps that indicate an effective diet therapy (reduction of body mass) on reducing self-esteem of women between the four stages It means that it leads to low self-esteem in women’s diet therapy (0.05&gt; P).

Immunological biomarkers changes during diet therapy in children with food allergy

Immunological estimating of different diet therapy types became currently very important. The aim of this work was to study the changes of immunological biomarkers during usage of formulas based on different sources of protein in children with food allergy and to assess and predict the effectiveness of diet therapy. The study included 66 children with food allergy in age from 1,5 months to 1,5 years. Observed children were divided into three groups depending on the type of formulas used. The dynamics of immunoregulatory substances (IL-5, TGF β 1, IL-10, IL-4, IL-2, IL-13, Eotaxin3), and allergenspecific IgE and IgG antibodies to cow’s milk protein, α-lactoglobulin, β-laktoglobulin, casein, soy and goat’s milk during diet therapy were evaluated. Observation duration was 28-30 days. The differences in the dynamics of the investigated parameters depending on the type of formula were revealed. Thus, the use of formula based on cow’s milk hydrolysates revealed their primarily effect on cytokines IL-2, IL-5 and growth factor TGF β ; the use of formula based on soy protein revealed combined effect on humoral immunity (allergen specific IgE and IgG antibodies) and IL-5; the use of formula based on goat milk characterized decreased levels of allergenspecific IgG antibodies to the cow’s milk protein and its fractions, reduced allergen specific IgE antibody concentrations to cow’s milk protein and α-lactoglobulin, decreased levels of IL-5, and, exclusive for this formula - reduction of IL-4 level. Accordingly, the assessment of immunological biomarkers can be used to personalize diet therapy in children with food allergy.

Correction of renal dysfunction under the influence of dietary management, exercises and lisinopril in patients with abdominal obesity and hypertension.

The purpose of research is to assess the impact of diet therapy, physical activity and an ACE inhibitor (lisinopril) on renal dysfunction in patients with abdominal obesity and hypertension. The study involved 14 patients with abdominal obesity, 2-3 degrees in BMI, hypertension, I-II stage, 1 degree, and severe insulin resistance. Men were 9 (64.3%) patients, women - 5 (35.7%) persons. The average age of the patients was 37,0±1,7 years, mean BMI - 44.7 [35.5; 46.5] kg/m2. BP was between 140-158/90-98 mm Hg. Diroton (lisinopril, Richter Gedeon Ltd, Hungary) was administered 1 time per day in the morning at a dose of 10 mg. The effect of diet therapy, exercise and lisinopril on parameteres of renal function (glomerular filtration rate, urine protein, urine albumin, urine β2 microglobulin, urine albumin / creatinine ratio, β2- microglobulin / urine creatinine ratio). After 6 months of treatment there was a significant decrease in body weight (p&lt;0.001) from 123.5 [110.0; 154.0] to 120.0 [105.0; 142.0] kg, with a BMI from 44.7 [35.5; 46.5] to 42.7 [33.9; 45.2] kg/m2 (p &lt;0.001) and waist from 118.0 [105.0; 142.0] to 116.0 [105.0; 135.0] cm (p&lt;0.05). Normalization of blood pressure was achieved in 71,4% (n=10) patients and a significant decrease in blood pressure - in 28,7% (n=4) persons in the 6th week of treatment. The level of GFR was significantly (p&lt;0.01) decreased from 202.2 [156.1; 254.6] to 200.3 [148.8; 220.6] ml/min, the level of β2-mg decreased from 3.4 [1.1, 4.8] to 2.8 [1.4; 3.6] mg/24 h, the β2-mg / Cr urine ratio - from 2.3 [0.9, 4.0] to 1.9 [1.0, 2.7] mg/g, which is a positive point prognosis of obesity-associated nephropathy in these patients. GFR normalized in 2 (14.3%) patients, β2-mg and β2-mg / Сr ratio - only in 1 (7.1%) patients. GFR decreased in 10 (71.4%) patients, β2-mg and β2-mg / Cr ratio – in 12 (85.7%) patients. It is the result of complex treatment of diet, exercises and lisinopril. A significant reduction (p&lt;0.01) of leptin from 95.6 [58.1; 141.9] to 87.0 [56.5; 132.6] ng/ml was observed in 13 (92.9%) patients. Patients in this group showed a significant decrease in lipid, carbohydrate metabolism and the level of insulin resistance. ACE inhibitors in combination with diet and exercises considering metabolic disorders are the complex treatment early manifestations of kidney damage in patients with abdominal obesity and hypertension.

Effects of weight loss diet therapy on anthropometric measurements and biochemical variables in schizophrenic patients

Background: Prevalence of obesity in schizophrenic patients is two to three times higher than in the general population and unhealthy dietary patterns, a sedentary lifestyle and antipsychotic medication use may contribute to the higher levels of obesity among schizophrenic patients. Aims: We evaluated the effects of diet therapy on weight loss, anthropometric and biochemical variables in overweight or obese (body mass index, BMI ≥ 27 kg/m2) female schizophrenic patients who use antipsychotic medications and in healthy volunteers. Methods: Primary demographic variables were collected via questionnaire; blood samples and anthropometric measurements were obtained. Personalized diet recipes were prepared and nutritional education was shared. We logged the physical activity of the patients and maintained food consumption records at 3-day intervals. Participants were weighed every week; anthropometric measurements and blood samples were collected at the end of the first and second months. Results: At the end of the study, reductions in body weight and other anthropometric measurements were statistically significant (P < 0.05). Reductions in body weight and BMI values for patient group were − 4.05 ± 1.73 kg and − 1.62 ± 0.73 kg/m2 and for the control group were − 6.79 ± 1.80 kg and − 2.55 ± 0.64 kg/m2, respectively. When compared with the patient group, reductions in the anthropometric variables of the control group were statistically significant (P < 0.05). Fasting glucose, blood lipids, albumin and leptin levels were decreased; insulin and homeostatic model assessment-measured insulin resistance (HOMA-IR) levels were increased insignificantly. Increases in the blood ghrelin levels for both groups were statistically significant (P < 0.05). Conclusions: Improvements to the diets of schizophrenic patient led to improvements in anthropometric measurements and biochemical variables and reduced the health risks caused by antipsychotic medications. Furthermore, we hypothesize that antipsychotic medications do not have any direct effect on leptin and ghrelin metabolism, and that changes in hormone metabolism may be attributable to changes in body weight.

Clinical effects of diet therapy plus oral probiotics plus Etiasa gas enema in ulcerative colitis

Clinical effects of diet therapy plus oral probiotics plus Etiasa gas enema in ulcerative colitis

Evaluation of the effectiveness of diet therapy in infants with gastrointestinal manifestations of food allergy

Background. The aim of the study was to evaluate the effectiveness of the diet therapy in infants with gastrointestinal manifestations of food allergy to cow’s milk protein. Materials and methods. 126 bottlefed infants aged from 1 to 12 months were examined for mucosal immunity and bowel microbiota parameters. Results. The changes of intestinal microbiota with in creased of conditionally pathogenic species (Klebsiella, Proteus, Citrobacter spp., etc.) with normal rates of anaerobic bacteria (bifido- and lactobacilli spp.) were revealed. sIgA levels in coprofiltrates in infants with gastrointestinal manifestations of food allergy were significantly reduced compared with the control group. Elevated C-reactive protein (CRP) levels compared with healthy children were obtained. Elevated levels of total IgE as well as allergen-specific IgE to cow’s milk proteins incoprofiltrates were estimated being the evidence of food sensitization. Using of adapted formulas based on the New Zealand goat milk with a complex of prebiotics in infants with gastrointestinal manifestations of food allergy led to normalization of intestinal microflora with reducing of Enterobacteriaceae spp. colonization. Positive changes in mucosal immunity were observed: increase of sIgA levels, decrease of total and allergen-specific IgE and CRP levels in coprofiltrates. Conclusion. The study demonstrated the usefulness of adapted formulas based on the New Zealand goat milk with a complex of prebiotics as diet therapy in infants with gastrointestinal manifestations of food allergy.

P41 ‐ Preexposure prophylaxis of infants’ food allergy

Methods We observed 100 “mother and child” pairs, which were divided into two groups. All children had AD, associated to cow milk protein (CMP) allergy. Lactating mothers from both groups were treated by diet therapy with the replacement of the cow milk to the New Zealand goat milk (1 group: 43 “mother and child” pairs) or non-dairy diet (2 group: 57 “mother and child” pairs). The effect of diet therapy was assessed by dynamics of clinical and immunological AD symptoms in infants after 1-3 months of treatment. Immunological effect of the therapy was assessed by the dynamics of levels of allergen-specific IgE and IgG to CMP, casein, b-lactoglobulin and goat milk protein which were measured by uncompetitive immunoenzyme assay on special test-systems from Allergopharma (Germany). The level of IFN-g, IL-2, IL-4, IL-5, IL-12, IL-13 was measured by immunoenzyme ELISA method.

ASSESSMENT OF CLINICAL-IMMUNOLOGICAL EFFECTIVENESS OF COMPLEX THERAPY OF INFANT ATOPIC DERMATITIS

The work is aimed at assessment of clinicalimmunological effectiveness of diet therapy based on hypoallergenic formula used for infants with atopic dermatitis. 167 infants with atopic dermatitis associated with cow’s milk protein allergy have been put included under outpatient observation. 52 infants of from the first group were being fed on whole goat milk’s formula, 56 infants of from the the second group — cows’ milk protein hydrolysate and 59 infants of the from the third groupsoy’s isolate formula. The age of infants was 2, 5—9 months. The duration of observation was 3—12 months. Complex therapy has been conducted over the infants and it contained antiallergenic treatment and diet therapy with hypoallergenic baby formula. The majority of patients showed improvement due to applied treatment. The first group’s infants with positive clinical dynamic showed decrease of specific IgG and IgE to cow’s milk protein, casein, β-LH and soy’s protein. The infants of the second and third groups also showed decrease of specific IgG and IgE to cow’s milk protein, casein, β-LH, soy’s protein and goat’s milk at clinical remission. All the infants with positive clinical effect showed trend to decrease of IL12, IL13, sICAM1 and sEselectin’s concentration in the blood stream, that can indicate on leukocyte adherence inhibition and cell migration to the area of inflammation. Based on the results of research, we can conclude that replacement of one product with another one by taking into account their clinicalimmunological effectiveness as the method of treatment the of atopic dermatitis patients let us optimize infant diet therapy.