Effect Of Collection Techniques Research Articles

English

The experiment was undertaken to study the effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation (IVM) and in vitro fertilization (IVF) of goat oocytes. COCs were collected by three techniques viz. puncture, slicing and aspiration of goat ovaries obtained at slaughterhouse. It was observed that, the total number of COCs/ovary as well as the number of abnormal COCs/ovary were significantly higher (p 0.05) on in vitro maturation and fertilization of goat oocytes as long as normal quality COCs was used. Key words : Collection techniques, cumulus oocyte complexes (COCs), in vitro maturation (IVM), in vitro fertilization (IVF).

Effects of collection technique and storage conditions on the stability of coagulation factors in frozen equine plasma

Effects of collection technique and storage conditions on the stability of coagulation factors in frozen equine plasma

Effects of methods of collection and storage on nutrients in human milk

The effects of collection technique, storage container, and the duration and temperature of storage on selected nutrient concentrations in mature human milk were evaluated. Milk samples were collected during the fourth week of lactation from women 20–35 years of age by hand expression or suction. Greater volumes and fat concentrations were observed in milks collected by suction. Vitamin A, zinc, iron, copper, sodium, and protein nitrogen concentrations were not affected by storage of milk in either pyrex or polypropylene containers for up to 24h. The storage temperature had a significant effect on protein nitrogen and ascorbic acid concentrations. These findings indicate that collection methods and storage procedures used for comparatively brief periods will affect the concentrations of selected nutrients of mature human milk. Specific recommendations are made for the collection and storage of milk.