Effect Of Cinnamon Essential Oil Research Articles

Antifungal effect of cinnamon essential oil against Penicillium oxalicum on rice noodles.

Plant essential oils have been extensively investigated for their application in food industry due to their broad antimicrobial spectrum and safety. However, rare studies investigated their application in decontaminating rice noodles from fungal contamination. In this study, the cinnamon essential oil was screened out among 12 species of plant essential oils, and its antifungal activity against Penicillium oxalicum isolated from rice noodles was investigated. Our study revealed that cinnamon essential oil inhibited the spore germination in a concentration-dependent manner, and a dosage of 0.025% (v/v) could entirely disable the spore germination. The disruption of the fungal plasma membrane was evidenced by the change of plasma membrane permeability and the leakage of cellular components. The cinnamon essential oil in vapor phase (0.00625% [v/v]) could totally inhibit the growth of fungi inoculated on rice noodles. In addition to the potential application in inactivating fungi germination on rice noodles, this study also demonstrated the feasibility of cinnamon essential as an environmental disinfectant. This study is the first report that cinnamon essential oil has been studied for decontaminating rice noodles from fungal contamination with P. oxalicum, which not only broadens the application field of plant essential oil but also provides an alternative approach for rice noodle preservation.

Comparative study of a liposome and emulsion system with cinnamon essential oil on the quality and proteolysis of refrigerated minced pork.

Essential oils have been recognized for their strong antibacterial property, making them an innovative approach for preserving meat. However, their chemical instability and direct impact on meat proteins limit their application. To overcome these limitations, various loading systems have been explored. This study aimed to compare the effect of cinnamon essential oil (CEO) loaded in a liposome and emulsion system on the proteolysis of minced pork and to evaluate the advantages of each delivery system in preventing microorganism-induced quality deterioration of meat. Minced pork treated with CEO-liposomes exhibited lower pH, total volatile basic nitrogen (TVB-N), and total viable count (TVC) values than CEO-emulsions and provided better protection against microorganisms. SDS-polyacrylamide gel electrophoresis (PAGE) analysis confirmed that CEO-liposome was more effective in protecting proteins from degradation. Moreover, CEO-liposome produced lower amount of bitter amino acids and harmful biogenic amines. Antibacterial mechanisms indicated that CEO-liposome exhibited a stronger inhibitory effect against major spoilage bacteria in meat products by increasing cell membrane permeability. The membrane damage was further supported by an increase in conductivity and the leakage of nucleic acids. Compared to the CEO-emulsion system, CEO-liposome emerged as an effective preservative for minced pork. These results provided important theoretical support for using a bioactive compound delivery system to prevent microorganism-induced quality deterioration in meat.

Antibacterial effect of cinnamon essential oil in combination with traditional antibiotics

Antibacterial effect of cinnamon essential oil in combination with traditional antibiotics

Cinnamon essential oil vapor alleviates the reduction of aroma-related volatiles in cold-stored "Feicheng" peach using HS-GC-IMS.

"Feicheng" peach is popular for its unique aroma, but its defect of being highly sensitive to chilling injury (CI) often leads to aroma loss and internal browning. Essential oils (EOs) are often used to enhance the antioxidant capacity of plants and fruits, as well as to trigger their defense against biotic/abiotic stresses. This study aimed to examine the effect of cinnamon essential oil (CEO) vapor treatment on the aroma quality of peach fruit during cold storage using HS-GC-IMS. The results showed that 50 μL/L CEO vapor reduced the severity of internal browning (IB) in peaches at the stage of 7 ~ 21 d during refrigeration (Significantly, the L* value was higher and the IB index was lower than that of control, p < 0.05). Meanwhile, the evident reduction or loss of aroma content caused by CI was restored to a higher level than the control (p < 0.05). Furthermore, CEO treatment promoted the release of aroma-related volatiles as evidenced by more propyl acetate, and the dimer of amyl acetate, isoamyl acetate, butyl acetate detected than that on harvest day and no-treated group after 21 d of cold storage plus 2 d of shelf life. Genes of PpLOX1, PpLOX2, PpHPL1 and PpADH1 associated with aroma-related volatile biosynthesis revealed higher transcript abundance in peach fruits treated with CEO than the control (p < 0.05). Overall, our study demonstrated that CEO in vapor phase may be beneficial to alleviate the quality deterioration in aroma and flesh color of "Feicheng" peaches caused by CI, which lays a theoretical reference for maintaining postharvest quality of peach fruits.

Effects of cinnamon essential oil on the physiological metabolism of Salmonella enteritidis.

Food safety and health are the themes of today's society. As a class of foodborne pathogens, Salmonella enteritidis has become one of the common zoonotic pathogens. Because chemical preservatives have certain harmfulness and have been questioned, it is particularly important to find green and safe natural preservatives. The advantages of plant essential oils (EOs) are that they are green and safe, have a wide range of antibacterials, and are not easy to form drug resistance. In recent years, studies have found that EOs have excellent antibacterial activity, but their antibacterial mechanism has not been conclusive, which has certain limitations in their application in the food field. Cinnamon essential oil (CEO) extracted from dried cinnamon is a secondary metabolite of cells and a very important natural food flavor. More importantly, it is non-toxic to the human body and has been proven to have a good antibacterial effect, but its antibacterial mechanism is still unclear. Therefore, it was of great practical significance to carry out the research on the antibacterial mechanism of CEO on S. enteritidis. In this work, S. enteritidis was used as the test bacteria, and CEO was selected as the antibacterial agent to study the antibacterial mechanisms. By studying the physiological metabolism of S. enteritidis cells by CEO, the influence of CEO on the bacteriostatic mechanism of S. enteritidis was systematically elucidated. The study found that CEO treatment would reduce the activity of bacterial metabolism. It is mainly reflected in the following three aspects: first, the activity of key enzymes in TCA circulation is inhibited, thus affecting the respiration of S. enteritidis. Second, it affects the level of energy metabolism by inhibiting the content of adenosine triphosphate (ATP) and the activity of ATPase. Finally, it can affect the physiological metabolism of bacteria by inhibiting the metabolism of proteins and other substances. Therefore, this article was expected to provide a theoretical basis for the development of new natural food preservatives and the prevention and control of S. enteritidis.

Evaluation of the hypolipidemic effect of cinnamon essential oil in a model of acute damage induced by triton WR-1339

Evaluation of the hypolipidemic effect of cinnamon essential oil in a model of acute damage induced by triton WR-1339

Anxiolytic-like Effect of Inhaled Cinnamon Essential Oil and Its Main Component Cinnamaldehyde in Animal Models.

Aromatherapy is one of the most common safer alternative treatments for psychiatric disorders with fewer side effects than conventional drugs. Here, we investigated the effects of cinnamon essential oil (CIEO) inhalation on mouse behaviors by performing different behavioral tests. CIEO inhalation showed anxiolytic effects in the elevated plus maze test, as inferred from increased time spent in open arms and decreased time spent in closed arms. Moreover, the CIEO treatment enhanced social behavior by increasing the total contact number, time spent in the center, distance traveled in the center, and total distance in the social interaction test. However, CIEO inhalation did not have any effect on performance in the open field test, tail suspension test, forced swimming test, and Y maze tests. The microarray analysis indicated that the CIEO treatment downregulated 17 genes and upregulated 15 genes in the hippocampus. Among them, Dcc, Egr2, and Fos are the most crucial genes that are involved in anxiety-related biological processes and pathways, including the regulation of neuronal death and neuroinflammation. Gas chromatography/mass spectrometry analysis revealed that cinnamaldehyde is the main component of CIEO. Cinnamaldehyde recovered MK-801-induced anxiety-related changes in the electroencephalogram power spectrum in zebrafish. Taken together, our findings suggest that CIEO and its main component cinnamaldehyde have an anxiolytic effect through the regulation of the expression of genes related to neuroinflammatory response and neuronal death.

Antibacterial and antibiofilm activities of some plant essential oils and synergistic effects of cinnamon essential oil with vancomycin against Clostridioides difficile: invitro study.

The detection of resistant strains of Clostridioides difficile against existing antibiotics and the side effects led to the investigation of alternative agents. Inhibition zones of various essential oils to four strains of C.difficile and other Clostridium species ranged from 8·32 to 44·18 mm. The highest zone was observed in cinnamon and tea tree essential oils. and The MIC values varied between 0·39-25 (%, v/v). The main components were cinnamaldehyde (85·64%) in cinnamon essential oil, 4-terpineol (83·6%) was determined in tea tree essential oil. Additive effects were found between cinnamon essential oil and vancomycin and between cinnamon and tea tree essential oils, and the FICI values were 0·512 and 0·517, respectively. Both cinnamon and tea tree essential oils showed antibiofilm activities against all tested C. difficile strains at all tested concentrations. Essential oils may be used as a supplement in addition to treatment in the control of C. difficile-related diseases.

Effects of Cinnamon Essential Oil on Oxidative Damage and Outer Membrane Protein Genes of Salmonella enteritidis Cells.

Salmonella is an important pathogen causing food poisoning. Food safety and health are the themes of today′s society. As a class of food-borne pathogens, Salmonella enteritidis had become one of the common zoonotic pathogens. Cinnamon essential oil (CEO) had been reported as an antibacterial agent, but there are few studies on its antibacterial mechanism. This study investigated the effects of CEO on oxidative damage and outer membrane protein genes of Salmonella enteritidis cells. First, the reactive oxygen species content in bacteria treated with different concentrations of cinnamon essential oil was determined by fluorescence spectrophotometry, and the effects of superoxide dismutase (SOD), catalase (CAT) and superoxide dismutase (SOD), and catalase (CAT) and peroxidase (POD) were determined by the kit method. The activity of POD and the content of malondialdehyde (MDA) were investigated to investigate the oxidative damage of CEO to Salmonella enteritidis cells. By analyzing the effect of CEO on the Salmonella enteritidis cell membrane’s outer membrane protein gene expression, the mechanism of CEO′s action on the Salmonella enteritidis cell membrane was preliminarily discussed. The results showed that CEO treatment had an obvious oxidative damaging effect on Salmonella enteritidis. Compared with the control group, the increase in CEO concentration caused a significant increase in the bacteria ROS content. The observation technique experiment found that with the increase in CEO concentration, the number of stained cells increased, which indicated that CEO treatment would increase the ROS level in the cells, and it would also increase with the increase in CEO concentration, thus causing the oxidation of cells and damage. In addition, CEO treatment also caused the disruption of the balance of the cellular antioxidant enzymes (SOD, CAT, POD) system, resulting in an increase in the content of MDA, a membrane lipid metabolite, and increased protein carbonylation, which ultimately inhibited the growth of Salmonella enteritidis. The measurement results of cell membrane protein gene expression levels showed that the Omp genes to be detected in Salmonella enteritidis were all positive, which indicated that Salmonella enteritidis carried these four genes. Compared with the control group, the relative expressions of OmpF, OmpA and OmpX in the CEO treatment group were significantly increased (p < 0.05), which proved that the cell function was disturbed. Therefore, the toxicity of CEO to Salmonella enteritidis could be attributed to the damage of the cell membrane and the induction of oxidative stress at the same time. It was speculated that the antibacterial mechanism of CEO was the result of multiple effects. This work was expected to provide a theoretical basis for the development of new natural food preservatives and the prevention and control of Salmonella enteritidis.

Effect of acetic and citric acid solvent combination with cinnamon oil on quality of edible packaging from chitosan

Chitosan is one of the fishery by-products, which is extracted from shrimp and crab carapace. Chitosan can be processed into edible packaging. The nature of chitosan edible packaging is depended on the type of solvent used while extraction. Acetic acid produces a strong coating with good barrier properties but less elastic, while citric acid produces an elastic layer but has relatively low barrier properties and weak coating. Cinnamon essential oil can inhibit bacterial growth. This study aimed to obtain the best proportion combination of acetic and citric acid solvents which can improve mechanical properties and permeability of the edible packaging, also determine the effect of cinnamon essential oil in inhibiting Salmonella and S. aureus bacteria. The experimental design used a completely randomized design (CRD), with solvent combination treatment in preliminary research initially followed by chitosan and cinnamon essential oil addition in the subsequent research. Data showed that the best edible packaging properties were obtained from a combination of acetic and citric acid (2:2) with 30.67 MPa tensile strength, 65.35% elongation, 0.0422 mm thickness, and moisture permeability 3.02x10−10 g.m.m−2.s−1.pa−1. The concentration of 1.5 g of chitosan with 1.5% cinnamon essential oil can produce an antibacterial with 10.15 mm inhibition zone diameter in Salmonella sp. and 9.53 mm in Staphylococcus aureus

Preparation and insecticidal performance of sustained-release cinnamon essential oil microemulsion.

To improve the utilization rate of cinnamon essential oil and compensate for the shortcomings of its easy decomposition and oxidation, the microemulsion of cinnamon essential oil was prepared using Tween 80 and anhydrous ethanol as surfactant and cosurfactant, respectively. The effects of the surfactant type, Km value, preparation temperature and aqueous pH on the quality of the microemulsion were studied via a pseudo-ternary phase diagram. The slow-release performance of cinnamon essential oil microemulsion and the control performance of the insect repellent package on the rice weevil were characterized. The results showed that, when Tween 80 was used as a surfactant and anhydrous ethanol was used as a cosurfactant, the Km value was 3:1, preparation temperature was 40 °C, aqueous pH was 5 and prepared cinnamon essential oil microemulsion was of the O/W type. The microemulsion had strong stability and a 81.5-nm concentrated particle size distribution, and possessed excellent embedding and sustained-release effects. The prepared insect repellent active package prolongs the use time and the effect of cinnamon essential oil. The repellent rate of the rice weevil was as high as 100% after 48 h, and the contact mortality and fumigation mortality rates of the rice weevil also reached 96.67% and 86.67%, respectively, after 96 h. The prepared cinnamon essential oil microemulsion and active packaging had a good sustained-release effect. © 2021 Society of Chemical Industry.

Antimicrobial properties and sensorial acceptability of edible antimicrobial films from seaweed (Kappaphycus alvarezii) and cinnamon (Cinnamomum zeylanicum) essential oil

Packaging of foods by using edible antimicrobial films (EAF) incorporated with essential oils is able to reduce the spoilage of food due to surface contamination. Essential oils are highly volatile and have strong aromas that can affect their function and consumer acceptance. This paper discussed the effect of cinnamon essential oil (CEO) concentrations on the antimicrobial activity and sensory acceptability of EAF produced from seaweed through a casting method. The CEO was added into the formulations at 10, 20, 30, 40 and 50 g/kg levels. The EAF showed antimicrobial activity against the tested microorganisms with the highest antimicrobial activity at the concentration of 40 g/kg CEO. However, based on the results obtained from a sensory evaluation using a 7-points hedonic scale, the overall acceptability was 3.23 which was lower than the minimum acceptability score of 4. Hence, the highest acceptable level of CEO in the EAF was 30 g/ kg. It is recommended that the CEO of 30 g/kg could be incorporated into edible film and act as an alternative method to extend the shelf life of packaged foods.

Effects of cinnamon essential oil on the physical, mechanical, structural and thermal properties of cassava starch-based edible films

The edible films were mainly made from oxidized hydroxypropyl cassava starch incorporated with cinnamon essential oil (CEO). The effects of CEO amount on the physical and mechanical properties of films were studied, and the structures of films with and without CEO were characterized. The results showed that the elongation at break, water resistance, water vapor transmission coefficient, as well as oxygen and ultraviolet barrier properties of the films (p < 0.05) significantly increased with addition of CEO, while the tensile strength of the films decreased. The field emission scanning electron microscopic (FE-SEM) images and infrared (IR) spectra showed that the CEO had good compatibility with other components and could be evenly dispersed in the film, which was conducive to the stable release of the active components. X-ray diffraction (XRD) patterns showed that the addition of CEO increased the crystallinity of the film, indicating that the compatibility and structural stability of the crystal structure of the film were improved. The thermogravimetric analysis results showed that CEO was beneficial to improve the thermal stability of the films. This study provided a potential to develop edible films from modified cassava starch with CEO.

The use of cinnamon oil as antibacterial agent to eliminate some antibiotic-resistant bacteria isolated from water sources

The use of cinnamon oil as antibacterial agent to eliminate some antibiotic-resistant bacteria isolated from water sources

Effects of cinnamon essential oil and Persian gum on preservation of pomegranate arils.

Given the high perishability of pomegranate arils, edible antimicrobial coating will enhance their shelf life and maintain their marketability. An antimicrobial coating was prepared using 1% (w/v) soluble part of Persian gum (PG) and different concentrations (0.25%, 0.50%, and 0.75% (v/v)) of cinnamon essential oil (CEO) to extend the shelf life of pomegranate arils. Microbiological, chemical, physical, and sensorial characteristics of coated and uncoated samples were evaluated at 7‐day intervals. Total anthocyanin (TAN), titrable acidity (TA), and ascorbic acid showed a decreasing trend, during the whole period of the storage. TAN, TA, and ascorbic acid decreased from 119.8 to 44.5 mg/L, 1.6% to 1.37%, and 682 to 140 mg/L, respectively. Firmness increased during the storage time, while total soluble solids (TSS, around 17.4 °Brix) and total phenolic compounds (TP, around 14.21 mg/100 ml) showed no significant changes with CEO concentrations. Coatings containing 0.5% and 0.75% CEO significantly prevented fungal growth on the samples at least for 3 weeks and 3 months, respectively. Optimization proved that 1‐week cold storage and 0.43% CEO could dramatically meet 80% of the research targets including maximum nutritional quality and freshness, as well preventing microbial spoilage. It was concluded that coating the pomegranate arils by PG and selecting an appropriate concentration of the CEO could considerably increase shelf life, marketability, and nutritional quality of pomegranate arils at a suitable and acceptable level.

Effect of cinnamon essential oil on morphological, flammability and thermal properties of nanocellulose fibre–reinforced starch biopolymer composites

Abstract The effect of different cinnamon essential oil (CEO) contents on flammability, thermal stability and morphological characteristics of nanocellulose fibre–reinforced starch biopolymer composites was studied. This sugar palm nanocellulose reinforced with sugar palm starch, containing 0–2% CEO, was prepared through solution casting technique. From scanning electron microscopy analysis, the cross-sections of the CEO-containing films showed appearance of micro-porous spots as micro-porous holes because of the occurrence of partial evaporation on the cryo-fractured surface as a result of the vacuum condition. Increment in CEO concentration resulted in increasing trend of the number and size of the micro-porous holes. Significant increase was observed in the thermal stability with the CEO loading when compared with neat composites. Besides that, increasing CEO loading also resulted in decrement of linear burning rate of the composites.

Exploring the effect of cinnamon essential oil to enhance the stability and safety of fresh apples

The core objective of the current study was to enhance the storage stability of apples with cinnamon essential oil edible coating. For this purpose, apples were coated with coating material containing different concentration of cinnamon essential oil. Coated apples were packed in polypropylene bags and to check the effect of edible coating stored at refrigeration at 5°C for 2 months. The coated apples were subjected to physiochemical analysis (moisture loss percentage, weight loss, color, total soluble solids, ascorbic acid, titratable acidity, and pH), microbial analysis (antifungal activity and antimicrobial activity), and sensory evaluation during storage after interval of 10 days for 2 months. During storage, a rapid decrease in all parameters was observed except in T3 (5% cinnamon essential oil). Edible coating contained T3 showed highest zone of inhibition against P. expansum and E. coli. Edible coating T3 prevent the spoilage and maintain the nutritional values of fresh apples. Practical applications The application of Edible coatings is increasing rapidly in the preservation of fruits and vegetables because of being environment friendly and safety of consumers. The edible coating with essential oils (EOs) is an effective approach to enhance the shelf life and preserve the nutritional quality of the fruits and vegetables commodities. The antimicrobial potential of Cinnamon Essential Oil make it as advantageous for the edible coating of fruits without affecting the quality and safety of the apple fruit.

Effects of Cinnamon (Cinnamomum spp.) in Dentistry: A Review.

Dental medicine is one of the fields of medicine where the most common pathologies are of bacterial and fungal origins. This review is mainly focused on the antimicrobial effects of cinnamon essential oil (EO), cinnamon extracts, and pure compounds against different oral pathogens and the oral biofilm and the possible effects on soft mouth tissue. Basic information is provided about cinnamon, as is a review of its antimicrobial properties against the most common microorganisms causing dental caries, endodontic and periodontal lesions, and candidiasis. Cinnamon EO, cinnamon extracts, and pure compounds show significant antimicrobial activities against oral pathogens and could be beneficial in caries and periodontal disease prevention, endodontics, and candidiasis treatment.

Influence of Cinnamon Essential Oil and Monensin on Ruminal Biogas Kinetics of Waste Pomegranate Seeds as a Biofriendly Agriculture Environment

The objective of present experiment was to compare the effect of cinnamon essential oil with monensin on the environmental biogas production kinetics of pomegranate seeds. Experimental treatments were: pomegranate seeds (control), pomegranate seeds with 12 mg monensin/kg dry matter, pomegranate seeds with 24 mg monensin/kg dry matter, pomegranate seeds with 150 mg cinnamon essential oil/kg dry matter and pomegranate seeds with 250 mg cinnamon essential oil/kg dry matter. The biogas produced by treatments incubation was recorded at 4, 6, 8, 12, 16, 24, 36, 48, 72, 96 and 120 h after incubation. The results showed that biogas production decreased numerically at 120 h after incubation in the treatment containing monensin (at 24 mg/kg dry matter) and cinnamon essential oil (at 150 and 250 mg/kg dry matter) compared to the control treatment. The addition of monensin and cinnamon essential oil had a significant effect (P < 0.05) on increasing partitioning factor and fermentation efficiency compared to control treatment. Concentration of ammonia nitrogen at 120 h of incubation was reduced (P < 0.05) by adding different levels of cinnamon essential oil and monensin at the level of 24 mg, and total volatile fatty acids were significantly reduced in treatments containing cinnamon essential oil. Concentrations of ammonia nitrogen and total volatile fatty acids showed an incremental effect (P < 0.05) with the addition of 12 mg of monensin. In conclusion, cinnamon essential oil and monensin can be used in an environmentally conducive and acceptable way to diminish biogas emissions from ruminants; therewith ameliorate environmental conditions. However, the cinnamon essential oil can be easily used in livestock diets to improve fermentation and reduce biogas production.

Preservation of traditional Chinese pork balls supplemented with essential oil microemulsion in a phase-change material package.

In this study, we evaluated the combined effect of cinnamon essential oil (CEO) microemulsions, and a temperature buffering package using phase-change material (PCM) microcapsules on the physicochemical property, lipid oxidation, and bacterial load of traditional Chinese pork balls (shi zi tou) during temperature fluctuation storage for 9 days. Transmission electron microscope characterization revealed that n-tetradecane microcapsules possessed a core-shell spherical shape with a size ranging from 300 to 600 nm. The use of n-tetradecane microcapsule packaging was found to maintain cold storage temperature efficiently. After 9 days of storage, the combination of CEO microemulsions with n-tetradecane microcapsules did not lead to changes in the color parameters of pork balls. At day 9, n-tetradecane microcapsules, used alone or in combination with CEO microemulsions, showed lower thiobarbituric acid reactive substances (TBARS) values than the control group, while their combination exhibited the lowest pH and 1,1-diphenyl-2-picrylhydrazyl (DPPH) values. Furthermore, the combination treatment retarded the growth of total plate count, lactic acid bacteria, Enterobacteriaceae, and Staphylococcus spp. after 4 and 9 days. The combinations of CEO microemulsions and PCM microcapsules could extend the shelf-life of cooked pork products, suggesting a feasible strategy for meat preservation. © 2020 Society of Chemical Industry.