Abstract The ever increasing heterogeneity and growing traffic volume has resulted in significant innovations and paradigm shifts within the telecom backbone networks. In order to cost-effectively respond to the diverse variety of traffic requirements having heterogeneous service demands, wavelength division multiplexed (WDM) optical networks have adopted the mixed line rate (MLR) strategy. In MLR networks, many wavelength channels with various line rates can co-exist within the same fiber which, however, raises many important design issues; one of them being the choice of the channel spacing. The quality of signal is affected by the channel spacing in terms of the bit-error rate (BER), which in turn affects the maximum optical reach of the lightpaths that depends on the line rates. In regard to the aforementioned, different methods can be adopted in order to set the width of the channel spacing, viz., (a) choice of a 50 GHz uniform fixed channel spacing specified by the ITU-T grid, (b) exploring various channel spacing values for different line rates so as to optimize the usage of the fiber spectrum, or (c) seek for an optimal value of the channel spacing which results in the minimum network cost. In the current work, we evaluate the MLR network cost for various channel spacings; hence, we find an optimal value of the channel spacing that leads to the minimum MLR network cost. The simulation results reveal that, for a MLR network, even with the assumption of uniform channel spacing, optimal values of the channel spacing for a minimum cost network can be identified.