Body weight support (BWS) and incline running (IR) are commonly used either during rehabilitation or during training separately, with many positive effects on athletes’ performance and rehabilitation. The aim of the present study was to investigate the interaction between bodyweight support and incline running on the electromyographic activity of the triceps surae and compare it to flat running. In eighteen healthy men (age: 20.3 ± 1.2 years, body weight: 70.2 ± 4.8 kg, body height: 179.6 ± 5.4 cm), the changes in electromyographic activity (EMGA) during a 10 min run with BWS (15% or 30% of body weight; in different occasions) and IR at 7%, as well as jumping performance and gait spatiotemporal parameters, were evaluated. A lower Rating of Perceived Exertion and a significant decrease in the size of the Vastus Lateralis (VL) (33.4%), Soleus (SOL) (17%), and Gastrocnemius Lateralis (GL) EMGA (28.5%, p < 0.05) but not in Gastrocnemius Medialis (GM) (10.5%, p > 0.05), was observed during BWS30% at 7% slope compared to flat running. Also, low-frequency fatigue of the quadriceps was induced only after running without BWS on a 7% slope (p = 0.011). No changes were found in jumping performance (p = 0.246) and gait spatiotemporal parameters (p > 0.05) except for flight time (p < 0.006). In conclusion, running with a slope of 7% and 30% of BWS can result in EMG activity comparable to that observed during level running. This method can also be used in prevention and rehabilitation training programs without creating fatigue.
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