Effect Of Ascorbic Acid Supplementation Research Articles

Effect of ascorbic acid supplementation on skin wound healing

Ascorbic acid is essential for proper synthesis of collagen fiber and intercellular.ground substance of connective tissueObjective of this study was to determine the effect of ascorbic acid supplementationmg once daily for l0 days in the treatment of wound skin incision about 2cm in555.length and 4mm in depthThirteen albino rabbit were used in this study (eight rabbits represent experimental.)group while five represent as a control groupThe result of general histological observation revealed rapid and active healingprocess through complete epithelization of the surface of incision site and colla enousg . .fibrous tissue formation in the incision wound of animals with ascorbic acidadministration while in control group incomplete epithelization and incomplete healing of.connective tissue of skin woundIt was concluded that the administration of ascorbic acid might be sufficient to promote wound healing

The Effect of Ascorbic Acid Supplementation on the Time of Healing of Rats Submitted to Neurosurgical Procedures

Abstract Introduction Vitamin C is an essential nutrient for both humans and rats and has been noted for its beneficial properties, among them, healing. Objective To verify the effect of oral and subcutaneous vitamin C supplementation on the healing time of surgical wounds of rats skulls. Statistical Methodology Thirty male Wistar rats were divided into 3 groups: 10 from the control group (GI), 10 from the group treated with oral vitamin C (GII), and 10 from the group treated with subcutaneous vitamin C (GIII). Vitamin C was administered to GI and GIII animals from the 3rd to the 7th postoperative day, totaling 10 days of administration at a dose of 100 mg/kg/day. On the 4th day of the study, the rats were submitted to a surgical procedure consisting of a 2-cm incision of the skin of the animals' heads and suturing with single stitches. After a determined period, the rats were killed and submitted to the collection of material for study by the picrosirius red technique for the evaluation of collagen types I and III, the degree of hematoxylin and eosin healing, and the rate of contraction of the wound on subsequent days. The results were described in averages, medians, minimum and maximum values, and standard deviations. For the comparison of the three groups, the analysis of variance with one factor (one-way ANOVA) or Kruskal-Wallis non-parametric test was used. The normality of the variables was evaluated by the Shapiro-Wilk test. Values of p < 0.05 indicated statistical significance. The data were analyzed using the IBM SPSS Statistics for Windows, v.20.0. software. (IBM Corp., Armonk, NY, USA). Results The amount of collagen type III was higher in the groups that received vitamin C, however, without significant difference (n = 0.292). In relation to the rate of contraction of the surgical wound, it was higher in the groups treated with vitamin C, with a significant difference between groups I and II (p = 0.001), and between groups I and III (p < 0.001). No significant difference was found between the groups that were treated with vitamin C (p = 0.227). Conclusion Healing was more effective in the groups treated with vitamin C than in the group that did not receive vitamin supplementation. There was no significant difference in healing between the groups receiving oral or subcutaneous vitamin C.

Effect of Ascorbic Acid Supplementation on Fecal Immunoglobulin A in Japanese Black Calves

Effect of Ascorbic Acid Supplementation on Fecal Immunoglobulin A in Japanese Black Calves

Evaluation effects of ascorbic acid leads to activate and induce osteogenic protein marker expression: in silico and in-vitro study

Background: Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into several cell types including chondrocytes and osteocytes. The process of differentiation into such tissues is aided by transcription factors and collagen gene markers. Collagen biosynthesis is influenced by the presence of vascular endothelial growth factor (VEGF) and ascorbic acid cofactors of the prolyl hydroxylase 2 (PHD2) enzyme found in human cell receptors. The focus of this study is evaluation of how in silico methods can elucidate the effect of ascorbic acid on PHD2 and its expression. This study aimed to evaluate the role of ascorbic acid in its interaction with PHD2 (in silico) and the expression of type 1 collagen (COL1), type 2 collagen (COL2), and VEGF. Methods: The technique of in silico molecular docking consisted of three steps: preparation of PHD2 receptors using AutoDock tools, preparation of ligand ascorbic acid using AutoDock tools, and molecular docking using AutoDock Vina. The results of in silico molecular docking were visualized using pyMOL and BIOVIA discovery studio. Furthermore, in vitro MSCs from adipose tissue were isolated and characterized based on protein marker expression. The effect of ascorbic acid supplementation (50 and 100 mg/mL) on stem cell differentiation was evaluated based on the mRNA ratio of VEGF/COL1, VEGF/COL2, and COL2/COL1. Results: Molecular docking of ligand ascorbic acid with PHD2 resulted in nine poses. The third pose was chosen because it had an affinity of -5.7 kcal/mol and a root-mean-square deviation (RMSD) of 1.96, indicating a valid docking result. Meanwhile, the 2D conformer showed van der Waals bonds on Tyr303 and Try329 residues. MSCs were differentiated into adipocytes, chondrocytes, and osteocytes. Regarding the marker ratio of gene expression, at 50 mg/ml ascorbic acid there was an increase in the VEGF/COL1 and VEGF/COL2 ratios and a decrease in the COL2/COL1 ratio compared with the control; at 100 mg/ml ascorbic acid there was a decrease in both ratios (VEGF/COL1 and COL2/COL1), and the VEGF/COL2 ratio continued to increase. Conclusions: In silico molecular docking between PHD2 and ascorbic acid, which was studied via bioinformatics, resulted in a stable interaction with good ligand position. Further in vitro studies would support the influence of ascorbic acid on transcription factors, collagen gene markers, and the differentiation of MSCs.

Effect of ascorbic acid supplementation on pulmonary functions in healthy adults: a randomized controlled pilot study.

The fact that oxidative stress plays an important role in the pathogenesis of various pulmonary diseases is supported by the beneficial effect of antioxidants. It is also well known that an altered oxidant-antioxidant balance after the age of 35 years increases the susceptibility to develop obstructive lung diseases later in life. Given this, the present study was designed to evaluate the effect of antioxidant supplementation on lung functions in healthy adults after the age of 35 years. Persons of age≥35 years (n=45) were randomized into three arms (each comprising 15 participants) to receive either no intervention (NI arm), ascorbic acid 250mg daily (AA250 arm), or ascorbic acid 500mg daily (AA500 arm) for 6weeks. Forced vital capacity (FVC), forced expiratory volume in 1s (FEV1), FEV1/FVC, and peak expiratory flow (PEF) were measured at baseline and 6weeks. Persons of age group (20-30 years) were also enrolled in the study to compare their lung functions and cardiovascular parameters at baseline with those≥35 years of age. All the adverse events experienced by participants were recorded. Baseline pulmonary functions were found to be comparable among the three study arms and compared to≥35 years age group, these parameters were found to be better in the younger age group (20-30 years). Most of the pulmonary functions were comparable among the three study arms at 6weeks. A significant improvement in PEF and % predicted PEF was noted in AA250 arm when compared to baseline values (p=0.049 and 0.026, respectively) and in participants with normal pulmonary functions when compared to those with reduced functions at baseline (p=0.059 and p=0.037). Although ascorbic acid did not affect most ofthe pulmonary functions in healthy adults, it improved PEF and % predicted PEF at a daily dose of 250mg. In this regard, it was found effective in individuals with normal pulmonary indices at baseline.

Effects of ascorbic acid supplementation on the performance of growing pullets

Two studies were conducted to evaluate the nutritional effects of supplementary ascorbic acid (AA) at levels of 0, 100, 200, and 300 mg/kg in the diets of growing pullets reared at different seasons (dry season and rainy season).240 eight weeks-old Harco pullet chicks were used in each trial, they were randomly assigned to each of the four dietary treatments in three replications of twenty chicks per replicate. Each trial lasted 12 weeks (84 days) during which data were collected and a 2 X 4 factorial arrangement was employed in the analysis of data collected. Both ascorbic acid and season significantly affected (P<0.05) growth rate with better growth rate reported in the dry season. Body weights at first lay exhibited a linear reduction with increasing concentration of ascorbic acid (P<0.05). Seasonal variation significantly affected (P<0.05) age and egg weight at first lay with higher values obtained in the rainy season. The cost-benefit analysis of supplementary ascorbic acid in the diet of experimental birds revealed that inclusion of the graded levels of ascorbic acid increased the cost of feed in a linear fashion across the dietary treatments. At the growing stage, cost of feed per kg weight gain was found to be lowest in birds fed 0 mg/kg AA. It was evident that at the growing stage, optimum response occurred with supplementation of 300 mg/kg AA.

Effects of ascorbic acid supplementation on immune status in healthy women following a single bout of exercise

This study was designed to determine the effects of ascorbic acid (AA) supplementation on immune status following a single bout of exercise. In a crossover design, 20 healthy sedentary women performed 30 min moderate-intensity cycling with (1000AA) or without (0AA) ingesting 1000 mg of AA daily for 1 week. Blood samples were taken immediately before, immediately after and 24 h post-exercise to determine the oxidative stress markers, immunophenotyping of peripheral blood lymphocytes and neutrophil phagocytic function. Moderate-intensity exercise in participants ranged in age from 21 to 23 years, showed no significant changes in oxidative stress markers in both cohorts. Plasma total creatine kinase was increased immediately after exercise and returned to baseline at 24 h post-exercise in both cohorts. Participants ingesting 1000 mg AA demonstrated significant higher level of plasma AA at pre-exercise and post-exercise as compared with the same time point in 0AA group. White blood cell and absolute neutrophil counts were increased immediately after exercise in both cohorts. Exercise resulted in increased lymphocyte count, CD4 + and CD8 + T cell counts immediately after exercise (p < 0.05) in 0AA group. AA supplement mitigated effects of exercise on CD4 + T cells. No significant change in neutrophil phagocytic function were observed when incubated with low or high concentrations of Candida albicans in both cohorts. These results suggested that a single bout of moderate-intensity exercise caused muscle injury with increased absolute CD4 + and CD8 + T cell counts, accompanied by a transient increase in neutrophil count, while their phagocytic function was not changed. However, a short-term AA supplementation does not show beneficial effects on exercise-induced changes in leukocyte subpopulations.

Ascorbic acid supplementation in type 2 diabetes mellitus: A protocol for systematic review and meta-analysis.

Background:Diabetes is one of the most common chronic diseases in the world. In recent years, with the continuous improvement of people's living standards and changes in dietary structure, the incidence of diabetes is gradually increasing. Studies have shown that ascorbic acid supplementation can reduce blood glucose, increase insulin synthesis and secretion, improve insulin resistance, and reduce the occurrence and development of complications of type 2 diabetes mellitus (T2DM). However, relevant studies have common problems such as the lack of large sample studies and low quality of included studies. Therefore, it is needed that we meta-analyze the clinical trials with high quality to elucidate the efficacy and safety of ascorbic acid supplementation in patients with T2DM.Methods:We will search randomized controlled trials published by PubMed, Embase, the Cochrane Library, Web of Science, and the Clinical Trials.gov website from inception to August 2020 on the effects of ascorbic acid supplementation on blood glucose, glycosylated hemoglobin, serum insulin, insulin resistance and other variables in T2DM patients with no language restrictions. The retrieval adopts the combination of medical subject headings and random words, and traces the references of the included literature to supplement the acquisition of relevant literature. Two researchers will independently screen the retrieved literature, extract the data and cross-check, and the Review Manage software V5.3.0 will be utilized for meta-analysis.Results:Our study will provide a high-quality and in-depth comprehensive analysis of the effects of ascorbic acid supplementation on blood glucose control, glycosylated hemoglobin and insulin resistance in type 2 diabetic patients.Conclusion:This systematic review and meta-analysis concerning randomized controlled trials of ascorbic acid supplementation for type 2 diabetic patients will provide a new direction and strong evidence to evaluate whether ascorbic acid supplementation is of benefit to glucose control and insulin resistance in T2DM.PROSPERO registration number:CRD 42019146826

Effect of Vitamin C Supplementation on Serum Ascorbic Acid Level and Liver Function Profile in Healthy Individuals

This study was conducted to explore the effects of ascorbic acid supplementation on serum liver function tests in healthy individuals. A total of 200 subjects were selected randomly. 100 were given ascorbic acid supplementation for 30 days. The other 100 were not given ascorbic acid supplementation, and serum ascorbic acid level and liver function profile was observed before and after intake of ascorbic acid in group A and without intake in group B. The liver function parameters determined were aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), serum total bilirubin, direct bilirubin, indirect bilirubin and serum protein (total protein, albumin and globulin). These parameters along with serum ascorbic acid were measured before and 30 days after vitamin C supplementation. Various parameters of liver function profile were improved swiftly when compared to other group which was not given ascorbic acid supplementation. While comparing the two treatment groups for 30 days, statistically significant improvement was seen in serum ascorbic acid levels (p&lt;0.001) along with improvement in some components of liver function profile such as serum ALT (p&lt;0.01), AST (p&lt;0.01), Total Bilirubin (p&lt;0.01) and Direct bilirubin (p&lt;0.001), Total Proteins (p&lt;0.01) and Albumin (p&lt;0.001) in group A as compared to Group B (without vitamin C supplementation intake). Conclusively, Liver Functions were significantly improved with vitamin C supplementation, giving the supportive evidence of the use of vitamin C as an antioxidant.

Possible Involvement of Vitamin C in Periodontal Disease-Diabetes Mellitus Association.

Ascorbic acid (vitamin C) is an important water-soluble vitamin found in many fruits and vegetables. It has well-documented beneficial effects on the human body and is used as a supplement, alone or in combination with other vitamins and minerals. Over recent years, research has focused on possible new therapeutic actions in chronic conditions including periodontal disease (PD). We conducted a systematic review on clinical trials from four databases (PubMed, Clinical Trials, Cochrane, Web of Science) which measured plasmatic/salivary levels of ascorbic acid in PD–diabetes mellitus (DM) association. Six studies were included in our review, three of them analyzing patients with different grades of PD and DM who received vitamin C as a treatment (500 mg vitamin C/day for 2 months and 450 mg/day for 2 weeks) or as part of their alimentation (guava fruits), in combination with standard therapies and procedures. Decreased levels of vitamin C were observed in PD patients with DM but data about efficacy of vitamin C administration are inconclusive. Given the important bidirectional relationship between PD and DM, there is a strong need for more research to assess the positive effects of ascorbic acid supplementation in individuals suffering from both diseases and also its proper regimen for these patients.

Effect of Ascorbic Acid Supplementation on Liver Function Tests in Hepatitis C Patients

An isolated liver function test is of little role in selection of liver disease because many harmful liver diseases may be correlated with normal levels of LFT’s. The outline of enzyme abnormalities in the perspective of patient’s commonly observed symptoms and laboratory data might be helpful in directing the subsequent diagnosis of liver diseases. Liver Function Tests (LFTs) are most generally used screening blood tests for assessment of different liver diseases and these tests provide a lot of evidence for disease processes whether for the purpose of investigation of supposed liver disease or help in observing the progress of disease action or simply by blood investigation. The evaluation of different liver enzymes simply gives diagnostic information on basic level whether patient’s principal disorder is actually hepatitis or cholestasis in source. However, it is necessary in various cases to evaluate LFTs with knowledge of liver functioning enzyme fractions. The objective of this study was to explore the effects of ascorbic acid supplementation on serum liver function tests in Hepatitis C patients. A total of 100 hepatitis C patients were selected randomly. 50 were given ascorbic acid supplementation for one month along with anti HCV treatment. The other 50 HCV patients took their normal anti HCV treatment without intake of ascorbic acid supplementation, and serum ascorbic acid level and liver function test parameters were observed before and after intake of ascorbic acid in both groups. The liver function parameters determined were aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), serum total bilirubin, direct bilirubin, indirect bilirubin and serum protein (total protein, albumin, globulin and A/G ratio). These parameters along with serum ascorbic acid were measured before and 30 days after vitamin C supplementation. Various abnormally elevated LFTs were also improved more rapidly when compared to other group which was not given ascorbic acid supplements for the period of one month. There was a significant change in levels of some liver function parameters before and after intake of ascorbic acid supplementation, and various abnormally elevated LFTs were also improved when compared to other group which was not given ascorbic acid supplements for the period of one month. The effect of Vitamin C supplementation was more marked on serum aminotransferase levels. After one-month use of ascorbic acid, serum alanine aminotransferase (p < 0.042) and serum aspartate aminotransferase (p < 0.000) levels were significantly decreased in hepatitis C patient group. In HCV group with ascorbic acid supplementation, serum total bilirubin (p < 0.046) and serum direct bilirubin (p < 0.048) were found to be less than the pre values when compared to HCV group without ascorbic acid supplementation. It was also observed that some of protein values were suggestively improved after intake of ascorbic acid supplementation.

Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)

The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth.

Effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exercise

BackgroundAscorbic acid is a water-soluble chain breaking antioxidant. It scavenges free radicals and reactive oxygen species (ROS), which are produced during metabolic pathways. Exercise can produce an imbalance between ROS and antioxidants, leading to oxidative stress-related tissue damages. This study was designed to determine the effects of ascorbic acid supplementation on circulating biomarkers of oxidative stress and muscle damage following a single bout of exercise.MethodsIn a crossover design with a 1 wk. wash-out period, 19 healthy women performed 30 min moderate-intensity cycling after ingesting 1000 mg of ascorbic acid (AA) or placebo. Blood samples were taken immediately before, immediately after and 30 min post-exercise to determine plasma albumin, total protein, glucose, oxidative stress and muscle damage markers.ResultsPlasma albumin and total protein levels increased immediately after exercise in placebo alongside slight reductions in glucose (p = 0.001). These effects were absent in AA cohort. Ferric reducing ability of plasma and vitamin C levels in AA cohort significantly increased after exercise (p < 0.05). Superoxide dismutase activity was significantly elevated after exercise (p = 0.002) in placebo but not AA. Plasma malondialdehyde did not change after exercise in placebo but was significantly decreased in AA (p < 0.05). The exercise protocol promoted slight muscle damage, reflected in significant increases in total creatine kinase in all subjects after exercise. On the other hand, plasma C-reactive protein and lactate dehydrogenase remained unchanged.ConclusionSupplementation with ascorbic acid prior exercise improves antioxidant power but does not prevent muscle damage.

Developmental lead (Pb)-induced deficits in hippocampal protein translation at the synapses are ameliorated by ascorbate supplementation.

BackgroundLead (Pb) is a persistent environmental neurotoxin and its exposure even in minute quantities has been known to induce neuronal defects. The immature brain is singularly sensitive to Pb neurotoxicity, and its exposure during development has permanent detrimental effects on the brain developmental trajectory and neuronal signaling and plasticity, culminating into compromises in the cognitive and behavioral attributes which persists even later in adulthood. Several molecular pathways have been implicated in the Pb-mediated disruption of neuronal signaling, including elevated oxidative stress, alterations in neurotransmitter biology, and mitochondrial dysfunction. Nevertheless, the neuronal targets and biochemical pathways underlying these Pb-mediated alterations in synaptic development and function have not been completely deduced. In this respect, recent studies have shown that synaptic signaling and its maintenance and plasticity are critically dependent on localized de novo protein translation at the synaptic terminals.Materials and methodsThe present study hence aimed to assess the alterations in the synapse-specific translation induced by developmental Pb exposure. To this end, in vitro protein translation rate was analyzed in the hippocampal synaptoneurosomal fractions of rat pups pre- and postnatally exposed to Pb using a puromycin incorporation assay. Moreover, we evaluated the therapeutic effects of ascorbic acid supplementation against Pb-induced deficits in synapse-localized protein translation.ResultsWe observed a significant loss in the rates of de novo protein translation in synaptoneurosomes of Pb-exposed pups compared to age-matched control pups. Interestingly, ascorbate supplementation lead to an appreciable recovery in Pb-induced translational deficits. Moreover, the deficit in activity-dependent synaptic protein translation was found to correlate significantly with the increase in the blood Pb levels.ConclusionDysregulation of synapse-localized de novo protein translation is a potentially critical determinant of Pb-induced synaptic dysfunction and the consequent deficits in behavioral, social, and psychological attributes of the organisms. In addition, our study establishes ascorbate supplementation as a key ameliorative agent against Pb-induced neurotoxicity.

Ascorbic Acid Supplementation Improves Postprandial Glucose and Blood Pressure in People with Type 2 Diabetes

The aim of the study was to investigate whether ascorbic acid (AA) supplementation improves postprandial glucose responses under free living conditions in people with type 2 diabetes (T2D). Thirty-one people with T2D (26 male, 5 female; aged 61.8 ± 6.8y; duration of diabetes 5.6 ± 4.6y [mean ± SD]) and stable glucose control (HbA1c 7.6 ± 0.7%) and blood pressure (SBP 139 ± 10; DBP 85 ± 9 mmHg) completed a randomized cross-over study involving 4 months of oral AA (2 x 500 mg/day) supplementation and 4 months of placebo supplementation (# ACTRN12616000276459). A minimum wash-out of 4 weeks separated treatments. Participants were affixed with continuous glucose monitors for 48h and consumed standardized meals pre and post supplementation. Intention to treat analyses were used to analyse data. Postprandial glucose area under the curve for 3.5h post meals (10.5h total) and the percent of day spent in hyperglycaemia (&amp;gt;10 mmol/l) were significantly lower by 31% and 11% (∼2.5h) after AA supplementation when compared to placebo, respectively (p&amp;lt;0.05). Significantly lower systolic (-6 mmHg) and diastolic (-4 mmHg) blood pressures were also found after AA supplementation compared to placebo. There were no effects of AA supplementation on percent of day spent in hypoglycaemia (&amp;lt;4 mmol/l), average 24h glucose or HbA1c. Treatments were well tolerated by participants, with no adverse effects reported. Capsule compliance was high for both treatments (AA: 93 ± 11%; placebo: 95 ± 7%). In summary, AA supplementation attenuated the daily post-prandial glucose response and the proportion of day spent in hyperglycaemia in people with T2D. AA also improved blood pressure in this group. This evidence collected under free-living conditions strengthens the argument for AA as a beneficial adjunct therapy to improve glycaemic control and cardio-metabolic health for people with T2D. Disclosure S. Mason: None. B. Rasmussen: None. G.D. Wadley: None.

Oral Hypoglycemic Agent and Ascorbic Acid Supplementation Therapy Synergistically Ameliorates Blood Glucose, Serum Lipid and Inflammatory Response in Type 2 Diabetes Mellitus Patients

Background: Type 2 diabetes mellitus is a chronic metabolic disorder where its prevalence has steadily been on the increase all over the world. It is characterized by increased generation of free oxygen radicals leading to oxidative stress. Hence supplementation of dietary antioxidants especially in the hypoglycemic therapy presents good prospects in its management. Aim: To evaluate the synergistic effect of ascorbic acid supplementation of a hypoglycemic agent on blood glucose, lipid profile and inflammatory response in Type 2 Diabetes Mellitus Patients. Methods: A total of 160 patients with newly diagnosed type 2 diabetes mellitus were included in the study. Patients were divided into 4 groups of 40 patients each. Anthropometric characteristics of all patients were measured at baseline. Control group received randomly glibenclamide 5mg daily, the first experimental groups received 5mg of glibenclamide with 600mg (0.6g) of ascorbic acid orally daily, the second group received 5mg of glibenclamide with 1,200mg (1.2g) of ascorbic acid orally while the third group received 5mg of glibenclamide with 1,800mg (1.8g) of ascorbic acid. The treatment lasted for nine weeks after which serum biochemical parameters of all patients were determined and recorded. Results: Our results showed that ascorbic acid supplementation caused a significant decrease (p 0.05) in between the groups. However, the HDL recorded a significant increase (p<0.05) in the treatment group supplemented with 1,800mg (1.8g) of ascorbic acid at the end of the 9th week compared to the control and the other ascorbic acid supplemented groups. In addition, there was a significant reduction in the serum level of C-reactive protein (CRP) in the 1.2g and 1.8g ascorbic acid supplemented groups at the end of the sixth week. Further progressive reduction at the end of the ninth week in all ascorbic acid supplemented group was also observed. Similar results were obtained for the other inflammatory biomarkers; leptin and adiponectin. Conclusion: Ascorbic acid (vitamin C) in a dose of between 1.2g and 1.8g per day as add on therapy to oral hypoglycemic agent synergistically causes an improvement in blood glucose, lipid profile and inflammatory response to the desirable levels hence mitigating the risks of type 2 diabetes mellitus complications.

Ascorbic acid serum levels are reduced in patients with hematological malignancies

In this paper we demonstrate that patients treated with chemotherapy and/or hematopoietic stem cell transplantation (HSCT) have highly significant reduced serum ascorbic acid (AA) levels compared to healthy controls. We recently observed in in vitro experiments that growth of both T and NK cells from hematopoietic stem cells is positively influenced by AA. It might be of clinical relevance to study the function and recovery of immune cells after intensive treatment, its correlation to AA serum levels and the possible effect of AA supplementation.

Effect of dietary supplementation of ascorbic acid on physiological parameters, body weight changes and reproductive performance of swamp buffaloes

To assess the effect of ascorbic acid supplementation on physiological parameters in buffalo viz., changes in body weight, dry matter intake and reproductive performances, eighteen Swamp buffaloes were divided into three groups. One group was fed with normal diet and other two groups supplemented with 10 and 15 g ascorbic acid animal−1 day−1. The group supplemented with 15 g ascorbic acid animal−1 day−1 had significantly lower respiration rate, pulse rate and rectal temperature as compared to the group supplemented with 10 or 0 g ascorbic acid animal−1 day−1. The dry matter intake kg−1 body weight, DMI per kg W0.75 was not affected by ascorbic acid supplementation. The body weight losses during winter were relatively less and the reproductive traits viz.., per cent of animals coming into oestrus, conception rate and number of services per conception improved at higher level of ascorbic acid supplementation. It may be concluded that dietary supplementation of ascorbic acid has favourable impact in maintaining the physiological parameters, body weight and improved reproductive traits of swamp buffaloes.

Effect of Ascorbic Acid Supplementation on Physiological and Blood Biochemical Parameters in Goats during Summer

Twelve adult male goats (1–1½ years old, 15–20 kg BW) were randomly divided into three groups namely T0, T1 and T2 comprising 4 animals in each group. Ascorbic acid was supplemented orally after dissolving in water @ 0, 50 and 100 mg/kg BW in T0, T1 and T2 groups, respectively, to see its effect on physiological, haematological and blood biochemical parameters in adult male goats during summer. The ambient temperature, relative humidity and temperature humidity index (THI) ranged from 24.5 to 37.16°C, 47.66 to 77.83% and 72.08 to 85.59, respectively, during the study period. Average rectal temperature (RT), pulse rate (PR) and respiratory rate (RR) ranged from 39.68±0.12 to 40.46±0.13°C; 58.75±1.93 to 72.25±4.20 and 107.33±1.53 to 118.13±1.69, respectively, which were significantly (P<0.01) reduced in T1 and T2 groups than control group (T0). However, no difference was seen between T1 and T2 groups. Similarly, overall mean haemoglobin (g/dL) and packed cell volume (PCV) value ranged from 8.37±0.30 to 10.23±0.55 and 24.08±1.31 to 28.25±1.04, respectively, which was higher (P<0.05) in T1 and T2 groups than control. There were no significant differences among the treatment groups for plasma glucose, potassium, alanine amino transferase (ALT) and aspertate amino transferase (AST). Mean plasma total protein and sodium level were significantly higher (P<0.01) in T1 and T2 groups than control group. However, mean plasma alkaline phosphatase (ALP) level significantly reduced (P<0.01) in T1 and T2 groups than control, but values were similar in T1 and T2 groups. From the present study it is concluded that supplementation of either 50 or 100 mg/kg BW of ascorbic acid is equally effective in reducing RT, PR and RR as well as increasing haemoglobin and PCV in goats during summer.

AMELIORATIVE EFFECT OF ASCORBIC ACID ADMINISTRATION AND CHILLED DRINKING WATER ON RAM LAMBS EXPOSED TO HEAT STRESS DURING SUMMER SEASON.

ABSTRACTThe ameliorative effect of ascorbic acid supplementation and drinking chilled water on heat stress was studied on ram lambs. Twenty male ram lambs were randomly divided into four equal groups of five animals each and nearly similar in age and weight. The 1st group served as control (CTR), the 2nd group was given cold water (10-15 °C) without adding vitamin C (G1), the 3rd group was supplemented with ascorbic acid at rate 2.5 g/h/d (G2), the 4th group was supplemented with ascorbic acid in addition to the cold water (G3). All animals exposed to the same managerial and climatic condition during the complete experimental period. Body weight (BW) recorded at the beginning, then at biweekly intervals until end of the experiment. Feed intake, feed conversion efficiency, water intake per unit body gain and water intake per unit feed intake were calculated for each lamb. Rectal and ear temperatures, respiratory and pulse rates were measured. Blood plasma samples were analyzed for some blood constituents (total protein, albumin, globulin, glucose, total cholesterol, ALT, AST, calcium (Ca++) and potassium (K+)). Total protein, albumin, globulin, glucose, cholesterol and calcium concentrations were significantly higher (P≤0.001), however liver enzymes AST and ALT were significantly lower (P≤0.001) in the blood plasma of treated lambs (G1, G2 and G3) than those in control group. Cumulative feed intake, body weight gain, and cumulative water intake were significantly higher (P≤0.01) in G3 than other three groups. Rectal temperature (P≤0.05), respiration rate and pulse rate (P≤0.01) increased significantly, while ear temperature was not significantly affected by treatment. In conclusion, ascorbic acid supplementation ameliorates the adverse effect of heat stress during summer season. The body temperature, respiration and pulse rates have decreased as a result of the use of cold water or supply of ascorbic acid. Therefore, availability of cold water is paramount to prevent heat stress in sheep, especially during periods of extended hot and humidity.