In today's ever-evolving and fiercely competitive business environment, the acquisition, assimilation, and effective utilization of knowledge have emerged as quintessential drivers of organizational success. Knowledge acquisition, which encompasses not just the accumulation of data but also the cultivation of tacit and explicit knowledge, plays a pivotal role in fuelling innovation, facilitating informed decision-making, and fostering adaptability. This study examines the impact of knowledge acquisition on organizational performance, with a null hypothesis guiding the inquiry. The research design adopted was survey research, A purposive technique was utilized. A sample size of 412 respondents from selected manufacturing companies in Oyo State, Nigeria, formed the study's subject group. Data collection was executed through a questionnaire, with a rigorous validation process. The findings of this study indicate a significant positive effect of knowledge acquisition on organizational performance. Enhanced knowledge acquisition is shown to empower organizations to achieve sustainability by developing new competencies that cascade across different organizational levels. This underscores the vital role of knowledge acquisition in positively influencing organizational performance. It was recommended that organizations invest in structured knowledge acquisition processes, mentorship programs, and knowledge management systems. Fostering a culture of continuous learning and knowledge sharing should also be a top priority, as these measures are poised to yield substantial enhancements in overall organizational performance and long-term sustainability.