Background and Study Aim. Response time is one of the important parameters affecting performance in sports. The aim of this study was to investigate the visual response time of the upper (hand) and lower (foot) extremities after a warm-up activity applied to female football and volleyball players. Material and Methods. Eleven female football players and eleven volleyball players, aged 19.27 ± 1.93 years, with a height of 164.90 ± 8.07 cm, body weight of 57.00 ± 6.01 kg, and 6.40 ± 2.36 years of sports experience, participated in the study as volunteers. Visual response times for the upper and lower extremities were measured before and after the warm-up protocol. The warm-up began with static stretching exercises for 2 minutes. This was followed by aerobic jogging for 2.5 minutes at a heart rate of approximately 140 beats per minute. After that, activities involving dynamic joint mobility were performed for 5 minutes. Finally, a 2.5-minute, three-stage sport-specific warm-up was completed. In total, the warm-up protocol lasted 17 minutes. Results. Volleyball players' upper extremity visual response time did not show significant differences before and after warm-up (Z = -1.561, p = 0.119). Significant differences were found in the upper extremity visual response time of football players before and after warm-up (t = 2.887, p = 0.016). No significant difference was found in the comparison of volleyball and football players' pre-warm-up (t = 1.905, p = 0.071) and post-warm-up (U = 43.000, p = 0.247) two-hand visual response times. A significant difference was found in the comparison of volleyball players' lower extremity visual response times before and after warm-up (Z = -2.674, p = 0.007). No significant difference was found in the comparison of lower extremity visual response times of football players before and after warm-up (t = 2.132, p = 0.059). A significant difference was determined in the comparison of lower extremity visual response times of volleyball and football players before warm-up (t = 3.307, p = 0.004) and after warm-up (U = 20.000, p = 0.008). Conclusions. The findings highlight the importance of sport-specific warm-up protocols in preparing athletes for optimal performance. Implementing appropriate warm-up strategies can therefore be a key factor in maximizing athletic performance and maintaining long-term physical health in female athletes.