Effects Of Various Treatments Research Articles

A compact setup for behavioral studies measuring limb acceleration

Behavioral studies contribute largely to a broader understanding of human brain mechanisms and the process of learning and memory. An established method to quantify motor learning is the analysis of thumb activity. In combination with brain stimulation, the effect of various treatments on neural plasticity and motor learning can be assessed. So far, the setups for thumb abduction measurements employed consist of bulky amplifiers and digital-to-analog devices to record the data. We developed a compact hardware setup to measure acceleration data which can be integrated into a wearable, including a sensor board and a microcontroller board which can be connected to a PC via USB. Additionally, we provide two software packages including graphical user interfaces, one to communicate with the hardware and one to evaluate and process the data. This work demonstrates the construction and application of our setup at the example of thumb acceleration measurement with a custom made glove and its use for research. Using integrated circuits, the size of the measurement devices is reduced to this wearable. It is simple to construct and can be operated easily by non-technical staff.

Understanding the mechanism of acupuncture in acute cerebral infraction through a proteomic analysis: protocol for a prospective randomized controlled trial.

Acute cerebral infarction (ACI), being the predominant form of stroke, presents challenges in terms of the limited effectiveness of various treatments in improving the neurological function. Although acupuncture shows promise in addressing ACI, the availability of high-quality evidence regarding its efficacy, safety, and underlying mechanism remains insufficient. In this study, we design a multicenter, prospective, single-blind, randomized controlled trial with the aim of evaluating the efficacy and safety of acupuncture for ACI, making an attempt to unveil the molecular mechanisms by proteomic. A total of 132 patients involving four hospitals will be randomized at a 1:1:1 ratio in the acupuncture group, control group, and sham acupuncture group. All the patients will receive basic treatment, and the patients in the acupuncture and sham acupuncture groups will also receive either acupuncture or sham acupuncture treatment, respectively, at six sessions each week for a 2 weeks period, followed by 3 months of follow-up. The primary outcome will be the change in the National Institute of Health Stroke Scale (NIHSS) scores after treatment. The secondary outcomes will include the Fugl-Meyer Assessment (FMA) scale scores and the Barthel Index (BI). Adverse events that occur during the trial will be documented. To discover differentially expressed proteins (DEPs) and their roles between the ACI subjects and healthy controls, we will also perform 4D-DIA quantitative proteomics analysis, and the DEPs will be confirmed by enzyme-linked immunosorbent assay (ELISA). This study was approved by the institutional review board of the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine (TYLL2023043). Written informed consent from patients is required. This trial is registered in the Chinese Clinical Trial Registry (ChiCTR2300079204). Trial results will be published in a peer-reviewed academic journal. The results of this study will determine the preliminary efficacy and safety of acupuncture in ACI patients and whether the mechanism of this form of non-pharmacologic stimulation is mediated by a novel therapeutic target for neurorehabilitation through our proteomic analysis. https://www.chictr.org.cn, identifier ChiCTR2300079204.

Galli-Galli Disease: A Comprehensive Literature Review.

Galli-Galli disease (GGD) is a rare genodermatosis that exhibits autosomal dominant inheritance with variable penetrance. GGD typically manifests with erythematous macules, papules, and reticulate hyperpigmentation in flexural areas. A distinct atypical variant exists, which features brown macules predominantly on the trunk, lower limbs, and extremities, with a notable absence of the hallmark reticulated hyperpigmentation in flexural areas. This review includes a detailed literature search and examines cases since GGD's first description in 1982. It aims to synthesize the current knowledge on GGD, covering its etiology, clinical presentation, histopathology, diagnosis, and treatment. A significant aspect of this review is the exploration of the genetic, histopathological, and clinical parallels between GGD and Dowling-Degos disease (DDD), which is another rare autosomal dominant genodermatosis, particularly focusing on their shared mutations in the KRT5 and POGLUT1 genes. This supports the hypothesis that GGD and DDD may be different phenotypic expressions of the same pathological condition, although they have traditionally been recognized as separate entities, with suprabasal acantholysis being a distinctive feature of GGD. Lastly, this review discusses the existing treatment approaches, underscoring the absence of established guidelines and the limited effectiveness of various treatments.

Influence of seasonal variation on steroidal content and in vivo post-coital antifertility activity of bark extracts of Caesalpinia pulcherrima Linn

BackgroundPrevious research has shown that seasonal variations in phytoconstituent levels eventually lead to alterations in the biological activities of the plant materials. The research assessed the effects of seasonal changes on β-sitosterol levels and the anti-fertility properties of two solvated bark extracts (ethanol and water) from Caesalpinia pulcherrima (CP) Linn. in female rats. Materials and methodsSoxhlet and hot reflux extraction with ethanol and water solvents were used to separate the active components from the barks. The phytochemical screening, characterization, and in-vivo antifertility activity in female rats were carried out to determine the presence of bioactive components. Two dosages of the extracts (200 and 400 mg/kg) were chosen based on the results of an acute toxicity investigation. Uterine tissue histopathology was performed to analyze the effects of various treatments. The data was statistically examined and p value less than 0.05 was considered to reflect the significance. ResultsThe extracts were characterized for the presence of beta-sitosterol using FT-IR, H1-NMR, and HR-Mass. The animals were able to withstand a dose of up to 2000 mg/kg without exhibiting any toxicological signs or symptoms. Based on in vivo investigations, an ethanolic extract of the bark showed 88.93 % and 77.21 % anti-implantation action at 200 and 400 mg/kg, while an aqueous extract showed 78.31 and 70.52 % activity at the same doses. When compared to the control, the anti-implantation and estrogenic actions of both extracts were shown to be statistically significant (p<0.001). The abortifacient effect in the rainy season ethanolic extract was linear and dose-dependent, as compared to the control (p<0.001). ConclusionThe findings of this study suggest that the presence of the phytosterol beta Sitosterol in Caesalpinia pulcherrima (CP) Linn. is responsible for its post-coital antifertility effect. It appears that the amount of beta-sitosterol in the plant, which may have an antifertility effect, is greatly affected by seasonal change.

The Effect of Virotherapy, Chemotherapy, and Immunotherapy to Immune System: Mathematical Modelling Approach

Medical research and therapeutic interventions continue to evolve, and one interesting area of study is the complex interaction among virotherapy, chemotherapy, and the immune system. Each treatment has its own advantages and disadvantages. In this study, a mathematical model was developed to describe how the immune system, tumor cells, and normal cells interact when all three types of therapy are used to treat cancer patients. To determine the effectiveness of various treatments, numerical simulations of eight different treatment strategies were performed. These simulations measured how much the concentration of immune cells, tumor cells, and normal cells decreased as a result of the treatment. Based on the numerical simulations performed, the application of the three types of therapy provided the greatest reduction (99%) in the concentration of tumour cells but also provided a significant reduction (68%) in the concentration of immune cells in the body.

Nephroprotective effects of Hydroalcoholic Extracts of Four selected Indian Medicinal Plants in Gentamicin-induced Nephrotoxic Wistar Rats

All the selected Indian medicinal plants, Ficus religiosa; Tinospora cordifolia; Moringa oleifera; Boerhaevia diffusa have profound pharmacological uses which have been proved by the study of Ayurvedic literature. An Aminoglycoside like Gentamicin is a broad-spectrum antibiotic in combination with beta-lactams for the treatment of systemic infection. The major risk associated with Gentamicin is nephrotoxicity and ototoxicity The present study was done to evaluate the comparative Nephroprotective effects of four selected Indian medicinal plant extracts in albino Wistar rats in Gentamicin-induced nephrotoxicity. Hydroalcoholic extracts of stem bark of Ficus religiosa, stem of Tinospora cordifolia, leaves of Moringa oleifera and roots of Boerhaevia diffusa were used at two doses levels of 200, and 400mg/kg respectively to evaluate Nephroprotective activity in albino Wistar rats by interfering Body weight, Blood urea nitrogen (BUN), Serum creatinine (Serum Creatinine), Serum total proteins, Urine volume, Urine creatinine, Creatinine clearance with Histopathology of Kidney. To observe the effect of various treatments on animals the rats were divided into Eleven groups, Six in each group. Urine creatinine, serum urea, and blood urea nitrogen were found to be significantly (P &lt; 0.001) increased in rats treated with only gentamicin; whereas treatment with the Hydroalcoholic extract of stem of Tinospora cordifolia and root of Boerhaevia diffusa reversed the effect of gentamicin indicating nephroprotective activity in compared to other plant extract treated animals. The Hydroalcoholic extract of Tinospora cordifolia and Boerhaevia diffusa at a dose level of 400mg/kg was found to be rich in flavonoids, polyphenolics, and other phytoconstituents which are responsible for good nephroprotective action in Gentamicin-induced nephrotoxicity in rats.

Nanoindentation study of human fingernail for determining its structural elasticity.

Human nails play an important role in transmitting force to the fingertips, and their mechanical properties are important indices. The nail has a three-layered structure consisting of top dorsal, middle intermediate, and under ventral plates, and its internal structure is believed to affect its mechanical properties. However, this has not been investigated in previous studies. The Young's moduli of the top, middle, and under plates were measured using nanoindentation, and a theoretical model was developed to estimate the structural elasticity for the bending deformation of human nails, which is an index describing the deformability of the nail without depending on its external dimensions. The structural elasticity of human nails was compared with that of human hair collected from the same person. The effect of the softening treatment on the nails was also evaluated. The Young's moduli of the top, middle, and under plates measured using nanoindentation were 2.9, 3.1, and 2.8GPa, respectively. The structural elasticity of the nail was estimated to be 2.9GPa, approximately 75% that of hair. Moreover, softening treatment with a urea cream reduced the structural elasticity of the nail to 70%. This paper proposed a method for estimating the structural elasticity of a human nail with a three-layered structure. This index is a mechanical property with "Pa" as a unit, and is useful for comparing deformability with the Young's modulus of other homogeneous materials or for investigating the effect of various treatments quantitatively.

Effect of Conservative Rehabilitation Interventions on Health-Related Quality of Life in Women with Upper Limb Lymphedema Secondary to Breast Cancer: A Systematic Review.

Breast cancer-related lymphedema (BCRL) of the upper limb is a very common condition in women undergoing breast cancer treatment; it can cause considerable alterations in the daily life of patients and a decrease in their health-related quality of life (HRQoL). Currently, there are many conservative therapies that try to palliate the symptoms, but the results are still controversial and there are still no globally accepted treatments. The purpose of this article is to determine the effect, according to the current available evidence, on HRQoL of different conservative interventions in the rehabilitation of BCRL in the upper limb in women. Eighteen articles that compared the effects of standard treatments, such as manual lymphatic drainage-based decongestive therapy or compression measures, and other newer treatments, including new technologies and other types of treatment programs, were reviewed. According to the results of this review, the most recommended modality for the improvement of HRQoL would be a complex decongestive technique without manual lymphatic drainage. Although there are clinical trials that have demonstrated the effectiveness of various treatments, the results of the positive effects on HRQoL remain highly controversial. There is a need to continue to develop studies to help guide therapeutic decisions that can promote HRQoL in women affected by upper limb BCRL.

Regulation of 20α-Hydroxysteroid Dehydrogenase Expression in Term Pregnant Human Myometrium Ex Vivo

Metabolic inactivation of progesterone within uterine myocytes by 20α-hydroxysteroid dehydrogenase (20α-HSD) has been postulated as a mechanism contributing to functional progesterone withdrawal at term. In humans, 20α-HSD is encoded by the gene AKR1C1. Myometrial AKR1C1 mRNA abundance has been reported to increase significantly during labor at term. In spontaneous preterm labor, however, we previously found no increase in AKR1C1 mRNA level in the myometrium except for preterm labor associated with clinical chorioamnionitis. This suggests that increased 20α-HSD activity is a mechanism through which inflammation drives progesterone withdrawal in preterm labor. In this study, we have determined the effects of various treatments of therapeutic relevance on AKR1C1 expression in pregnant human myometrium in an ex vivo culture system. AKR1C1 expression increased spontaneously during 48 h culture (p < 0.0001), consistent with the myometrium transitioning to a labor-like phenotype ex vivo, as reported previously. Serum supplementation, prostaglandin F2α, phorbol myristate acetate, and mechanical stretch had no effect on the culture-induced increase, whereas progesterone (p = 0.0058) and cAMP (p = 0.0202) further upregulated AKR1C1 expression. In contrast, culture-induced upregulation of AKR1C1 expression was dose-dependently repressed by three histone/protein deacetylase inhibitors: trichostatin A at 5 (p = 0.0172) and 25 µM (p = 0.0115); suberoylanilide hydroxamic acid at 0.5 (p = 0.0070), 1 (p = 0.0045), 2.5 (p = 0.0181), 5 (p = 0.0066) and 25 µM (p = 0.0014); and suberoyl bis-hydroxamic acid at 5 (p = 0.0480) and 25 µM (p = 0.0238). We propose the inhibition of histone/protein deacetylation helps to maintain the anti-inflammatory, pro-quiescence signaling of progesterone in pregnant human myometrium by blocking its metabolic inactivation. Histone deacetylase inhibitors may represent a class of agents that preserve or restore the progesterone sensitivity of the pregnant uterus.

A Retrospective analysis of peri-implant fractures: insights from a large volume clinical Study.

Peri-implant fractures (PIFs) are a concept in evolution for which different diagnostic criteria have been proposed and modified over time. They have not been extensively reported. PIFs are usually located in the distal part of previous osteosynthesis, where fragile bone is in contact with the rigid implant and are a significant complication after orthopaedic surgery, which requires careful management and treatment. This clinical study presents findings from a retrospective analysis of PIF's, providing description of characteristics and outcomes. They provide information about the associated risk factors and description of the effectiveness of various treatments. A retrospective analysis was performed in a clinical study involving patients with PIFs. Data from medical records, radiographic images, and surgical reports were collected and analyzed. The study included patients with refracture of pre-existing osteosynthesis admitted to the hospital between 2015 and 2022. Demographic information, fracture characteristics, surgical interventions, and post-operative outcomes were assessed. Each case was followed for a minimum of one year after surgery, and a critical analysis was performed by senior surgeons. Furthermore, the PIFs were classified according to the type of initial implant and the position of the new fracture in relation to the original implant. Between 2015 and 2022, out of 18,813 fractures treated at a Level 1 traumatology hospital, a total of 85 patients with PIF's were identified. Fracture characteristics varied, including location, fracture pattern, and implant type. Most of the PIFs, 71 cases (83.53%), occurred in the femur. Additionally, there were seven cases (8.24%) involving the humerus, four cases (4.71%) affecting the tibia/fibula, two cases (2.35%) in the radius, and one case (1.18%) involving the clavicle. Surgical management was the primary attitude in treatment. Various techniques were used, including the use of longer and stronger implants to achieve high-quality fixation, facilitating early rehabilitation and return to previous activities. Postoperative complications after surgery for PIFs were observed in 16 cases (18.8%). Peri-implant fractures (PIF) are a distinct topic and a challenging condition with a high rate of postoperative complications. Complications after the second surgery may be severe and life threatening in older patients. A classification can be useful to make decisions about the optimal treatment in different anatomic areas and type of implant used for primary osteosynthesis. Currently there are no standardized protocols for management, therefore each case should be carefully discussed and planned before surgery.

Treatments associated with all-cause mortality among children with primary brain and central nervous system tumors: a retrospective cohort study from the SEER database.

Evidence on treatment modalities and survival in childhood primary brain and central nervous system (CNS) tumors remains contradictory, with previous studies often lacking sufficient patient cohort sizes to assess the differences in histological subtypes. This cohort study based on a large population investigated the effects of various treatments on the mortality of patients with different histological types of primary brain and CNS tumors from the Surveillance, Epidemiology, and End Results (SEER) database. Data of demography, primary tumor site, histology, tumor grade and treatments from all pediatric patients with primary brain and CNS tumors were extracted in this retrospective cohort. The outcomes were overall, 1-, 5-, and 10-year all-cause mortality. Multivariate Cox proportional hazards models were to explore the associations of treatment with overall, 1-, 5-, and 10-year all-cause mortality, with hazard ratios (HRs) and 95% confidence intervals (CIs). Totally 10,994 children were included, with the mean age at diagnosis of 7.3 years, and the median follow-up time of 5.0 years. Of which, 2,003 (18.2%) were diffuse astrocytoma, 3,188 (29.0%) were embryonal tumors, and 3,691 (33.5%) were malignant glioma. Then 4,333 (39.41%) children died during the follow-up. For diffuse astrocytomas and malignant gliomas, patients who received all other treatments were associated with overall, 1-, 5- and 10-year all-cause mortality compared to those only received resection. Embryonal tumors patients receiving resection with radiation only and those receiving resection with both chemotherapy and radiation were associated with lower odds of overall, 1-, 5- and 10-year all-cause mortality compared to patients who only received resection. For ependymal tumors, no surgery/only biopsy with chemotherapy, resection with chemotherapy only, resection with both chemotherapy and radiation, and other treatments had increased risks of overall all-cause mortality compared with resection. The risk of 1-year all-cause mortality increased in ependymal tumors with treatment involving resection and radiation. However, resection with both chemotherapy and radiation was not significantly associated with the 1- nor 5-year all-cause mortality. Resection may be recommended for children with diffuse astrocytoma, ependymal tumors, and malignant glioma, while resection with radiotherapy or chemoradiation may be recommended for children with embryonal tumors.

Cinacalcet use in secondary hyperparathyroidism: a machine learning-based systematic review.

This study aimed to systematically review research on cinacalcet and secondary hyperparathyroidism (SHPT) using machine learning-based statistical analyses. Publications indexed in the Web of Science Core Collection database on Cinacalcet and SHPT published between 2000 and 2022 were retrieved. The R package "Bibliometrix," VOSviewer, CiteSpace, meta, and latent Dirichlet allocation (LDA) in Python were used to generate bibliometric and meta-analytical results. A total of 959 articles were included in our bibliometric analysis. In total, 3753 scholars from 54 countries contributed to this field of research. The United States, Japan, and China were found to be among the three most productive countries worldwide. Three Japanese institutions (Showa University, Tokai University, and Kobe University) published the most articles on Cinacalcet and SHPT. Fukagawa, M.; Chertow, G.M.; Goodman W.G. were the three authors who published the most articles in this field. Most articles were published in Nephrology Dialysis Transplantation, Kidney International, and Therapeutic Apheresis and Dialysis. Research on Cinacalcet and SHPT has mainly included three topics: 1) comparative effects of various treatments, 2) the safety and efficacy of cinacalcet, and 3) fibroblast growth factor-23 (FGF-23). Integrated treatments, cinacalcet use in pediatric chronic kidney disease, and new therapeutic targets are emerging research hotspots. Through a meta-analysis, we confirmed the effects of Cinacalcet on reducing serum PTH (SMD = -0.56, 95% CI = -0.76 to -0.37, p = 0.001) and calcium (SMD = -0.93, 95% CI = -1.21to -0.64, p = 0.001) and improving phosphate (SMD = 0.17, 95% CI = -0.33 to -0.01, p = 0.033) and calcium-phosphate product levels (SMD = -0.49, 95% CI = -0.71 to -0.28, p = 0.001); we found no difference in all-cause mortality (RR = 0.97, 95% CI = 0.90 to 1.05, p = 0.47), cardiovascular mortality (RR = 0.69, 95% CI = 0.36 to 1.31, p = 0.25), and parathyroidectomy (RR = 0.36, 95% CI = 0.09 to 1.35, p = 0.13) between the Cinacalcet and non-Cinacalcet users. Moreover, Cinacalcet was associated with an increased risk of nausea (RR = 2.29, 95% CI = 1.73 to 3.05, p = 0.001), hypocalcemia (RR = 4.05, 95% CI = 2.33 to 7.04, p = 0.001), and vomiting (RR = 1.90, 95% CI = 1.70 to 2.11, p = 0.001). The number of publications indexed to Cinacalcet and SHPT has increased rapidly over the past 22 years. Literature distribution, research topics, and emerging trends in publications on Cinacalcet and SHPT were analyzed using a machine learning-based bibliometric review. The findings of this meta-analysis provide valuable insights into the efficacy and safety of cinacalcet for the treatment of SHPT, which will be of interest to both clinical and researchers.

A Critical Review of the Prognostic and Predictive Implications of KRAS and STK11 Mutations and Co-Mutations in Metastatic Non-Small Lung Cancer.

The Kirsten rat sarcoma viral oncogene homolog (KRAS) and serine/threonine kinase 11 (STK11) co-mutations are associated with the diverse phenotypic and heterogeneous oncogenic subtypes in non-small cell lung cancer (NSCLC). Due to extensive mixed evidence, there needs to be a review of the recent KRAS and STK11 mutation literature to better understand the potential clinical applications of these genomic biomarkers in the current treatment landscape. This critical review highlights the clinical studies that have elucidated the potential prognostic and predictive implications of KRAS mutations, STK11 mutations, or KRAS/STK11 co-mutations when treating metastatic NSCLC across various types of treatments (e.g., immune checkpoint inhibitors [ICIs]). Overall, KRAS mutations are associated with poor prognoses and have been determined to be a valid but weak prognostic biomarker among patients diagnosed with NSCLC. KRAS mutations in NSCLC have shown mixed results as a predictive clinical biomarker for immune checkpoint inhibitor treatment. Overall, the studies in this review demonstrate that STK11 mutations are prognostic and show mixed results as predictive biomarkers for ICI therapy. However, KRAS/STK11 co-mutations may predict primary resistance to ICI. Prospective KRAS/STK11-biomarker-driven randomized trials are needed to assess the predictive effect of various treatments on the outcomes for patients with metastatic NSCLC, as the majority of the published KRAS analyses are retrospective and hypothesis-generating in nature.

Effects of Integrated Use of Organic Manure and Bio-fertilisers on Crop Productivity: A Case Study of Rice (Oryza sativa L.) Crop

The present study conducted entitled, “Effect of Integrated use Organic manure and Bio-fertilizers on crop productivity under Rice (Oryza sativa L.) crop". Involved field trial conducted during Kharif season of year 2016-17 followed by laboratory analysis of the plant and soil samples in the Department of Soil science &amp; Agriculture Chemistry, Udai Pratap Autonomous College, Varanasi (U. P). All grasses were removed from the experimental plots and soil samples have been taken from each replication plots at 30 DAT, 60 DAT and at harvesting. The experiment was conducted under randomized block design (RBD) with six treatment combinations. Treatments were replicated thrice making the total number of 18 plots. The effect of various treatments on dry matter production could be arranged in order of T6&gt;T3&gt;T5&gt;T4&gt;T2&gt;T1 and the values were 70.25, 62.15, 59.45, 43.40, 41.25 and 35.59 gm-1 row length, respectively. Application of fertilizers alone or in combination with F.Y.M increased grain and straw yield of rice significantly over control. Further, the yield was significantly superior under the use of organic manure and bio-fertilizers over the sole use of chemical fertilizers. On the basis of data, the superiority of the treatments may be arranged as T6&gt;T3&gt;T5&gt;T4&gt;T2 and T1. Like dry matter yield, rice grain and straw yield was also highest in treatment where 50% NPK was substituted through FYM to rice crop. The integrated use of fertilizers with FYM and bio-fertilizers might have added huge quantity of organic matter in soil that increased grain and straw yield. In general, higher number of tillers (15.25 m-1 row length), plant height (92.50 cm), dry matter at 60 DAT (70.25 gm-1 row length), grain yield (46.25 Qha-1 ) and straw yield (91.25 Qha-1 ) obtained with T6 treatment followed by T3&gt;T5&gt;T4&gt;T2&gt;T1 (control).

Early prediction of breast cancer based on the classification of HER‐2 and ER biomarkers using deep neural network

AbstractBackgroundOwing to the highly coarse chromatin, multi‐dimensionality of the histo image, irregularity of shape and size, texture and appearance, nuclei extraction is challenging. To address these complexities, a deep‐learning algorithm called a stacked sparse autoencoder was considered as a research factor in this study.Methods and MaterialsThis study focuses on detecting the epithelial regions and extracting high‐level features to segment the patches based on the nuclei and classify the biomarkers concerning the nuclei patches. We used 6,53,400 microscopic image patches of 363 patients sourced from the BreakHis database, of which 4,90,050 prominent image patches containing only nuclei were utilized for biomarker classification (essentially eliminating the non‐nuclei patches from 363 whole‐slide images (WSI)). Non‐nuclei patches were eliminated owing to an imbalanced class distribution. From the nuclei patches the biomarkers oestrogen receptor (ER) and human epidermal growth factor receptor‐2 (HER‐2) that are crucial for the early diagnosis of breast cancer are predicted based on the nuclei count and Nuclei intensity. These indicators aid in predicting the risk of breast cancer as well as the severity and potential effectiveness of various treatments. About 20%–25% of breast cancer cases have an overexpression of the protein HER‐2, which makes the tumour more aggressive and on the other hand, the majority of breast cancer cases express the protein ER.ResultsThe classifier finally classified if the nuclei detected based on the features were benign, malignant, or normal with an accuracy of 99.73%, using which early prediction is performed by extracting and classifying the biomarkers HER‐2 and ER. The overall classification rate for classifying HER‐2 and ER was 97.52%.ConclusionHER‐2 +ve was classified with an intensity above 23%, and total nuclei in the range of 150–1000 were termed ER‐positive. Based on these 40 HER‐2 +ve patients, 25 ER +ve patients were detected in 363 patients. From this observation, it is concluded that 25–40 patients are at risk of breast cancer in the next 5 years due to the cell proliferation rate of 7000.

Effects of Poultry Manure on the Growth, Physiology, Yield, and Yield-Related Traits of Maize Varieties.

Industries play a significant role in the improvement of lifestyle and in the development of a country. However, the byproducts from these industries are a source of environmental pollution. The proper use of the byproducts of these industries can help to cope with environmental pollution. Some byproducts have high nutritional content and are good for crop plants. The purpose of this research was to investigate the effect of different rates of poultry manure on the soil chemical properties, growth, and yield of maize. A pot experiment was conducted in the botanical garden of the Department of Botany, University of Sargodha, Pakistan to investigate the effect of various treatments of poultry manure (0, 25, 50, 75, and 100 g/pot) on the morphological, physiological, and yield attributes of two maize varieties, Pearl and MMRI. Treatment T1 was a mixture of soil and 75 g/pot poultry manure, T2 was a mixture of soil and 50 g/pot poultry manure, T3 was a mixture of soil and 25 g/pot poultry manure, and T4 was 100 g/pot poultry manure. Soil without any industrial byproduct (100% soil only) was used as the control (T0). The results revealed that the use of poultry manure enhanced the physical properties of the soil. Available P and soil organic matter were improved in soil amended with poultry manure. It is evident from the results that the vegetative growth of both maize varieties was significantly enhanced by growing in soil amended with poultry manure as compared to their respective control. Similar responses were also recorded for the physiological attributes of leaf area, photosynthetic rate, transpiration rate, stomatal conductance, and water use efficiency of both varieties. Yield and yield-contributing traits of both maize varieties were significantly improved by growing plants in soil amended with 50 and 75 g/pot of poultry manure. It is also inferred that the use of 50 g/pot poultry manure in soil amendment is an eco-friendly and economically effective option for maize growers of arid and semiarid regions to enhance the kernel yield and profit per annum. Poultry manure could be useful to ameliorate the adverse effects of salinity stress on all parameters, particularly the grain yield. Furthermore, this would be a useful and economical method for the safe disposal of byproducts.

Efficacy and Safety of intravenous monoclonal antibodies in patients with moderate-to-severe active Graves'ophthalmopathy: a systematic review and meta-analysis.

The effects of various treatments on Graves' ophthalmopathy (GO) have been studied. As monoclonal antibodies (mAbs) have been proposed for the treatment of moderate to severe GO, direct comparisons between different mAbs are lacking.We therefore conducted this meta-analysis to objectively compare the efficacy and safety of intravenous mAbs. To identify eligible trials, references published before September 2022 were electronically searched in PubMed, Web of Science, Pubmed, Embase,Cochrane Library, CBM, CNKI,Wan-Fang and ICTRP databases.The Newcastle-Ottawa scale (NOS) and the Cochrane Risk of Bias Assessment Tool were used to assess the risk of bias of the original studies.The primary and secondary outcomes were the response and inactivation rates, with the secondary outcomes being the clinical activity score (CAS),the improvement of proptosis and diplopia improvement,and the adverse event rate. Publication bias was evaluated, along with subgroup and sensitivity analyses. A total of 12 trials with 448 patients were included. The meta-analysis showed that TCZ (tocilizumab) was most likely to be the best treatment in terms of response according to indirect contrast, followed by TMB (teprotumumab) and RTX (rituximab).TCZ, followed by TMB and RTX, was also most likely to be the best treatment in terms of reducing proptosis. In terms of improving diplopia, TMB was most likely to be the best treatment, followed by TCZ and RTX.TCZ was the highest probability of safety, followed by RTX and TMB. Based on the best available evidence,TCZ should be the preferred treatment for moderate to severe GO.In the absence of head-to-head trials,indirect comparisons of treatments are routinely used to estimate the effectiveness of the treatments of interest. In addition,the optimal dose and potential mechanism of action of monoclonal antibodies remain to be established,and it is encouraging that the treatment paradigm for GO may change in the future.This study was designed in accordance with the Preferred Reporting Items for conducting Systematic Reviews and Meta-Analyses (PRISMA)(27). http://www.crd.york.ac.uk/prospero, identifier CRD42023398170.

The effect of various washing methods on pesticide residues, toxic and essential elements removal in rice.

This study examined the effects of various treatments on removing pesticide residues and toxic elements in rice. In parallel, nutritional elements, magnesium (Mg), potassium (K), and phosphorous (P), were measured to investigate the effect of these washing treatments on the nutritional value of rice. A naturally contaminated rice sample containing five widespread used pesticides (azoxystrobin, buprofezin, carbendazim, and propiconazole) and toxic elements, arsenic (As), cadmium (Cd), and essential elements, was washed using several washing agents, including boiling water, 5% sodium bicarbonate (baking soda), 5% acetic acid (vinegar), 5% citric acid, and 5% sodium chloride (salt). The washing method was chosen based on its availability and widespread usage; soaking for 10min was assumed to be reasonable. Our results showed that using 5% acetic acid significantly reduced azoxystrobin by 63%, buprofezin by 70%, carbendazim by 75%, and propiconazole by 61%. However, As and Cd were significantly reduced in sodium chloride by 57% and 32%, respectively. Furthermore, a significant reduction in essential nutrient elements was found in Mg (42%), K (37%), and P (23%) when rice was treated with 5% citric acid. Overall, washing agents reduced analytes in the following manners pesticides, toxic elements, and essential elements when using acetic acid, sodium chloride, and citric acid separately.

Comparison of survival outcomes of different treatment modalities for patients with primary splenic diffuse large B cell lymphoma.

Primary splenic diffuse large B cell lymphoma (DLBCL) is one of the most common primary tumors in the spleen, while its prevalence is relatively low. Recently, there has been an increase in the incidence rate of primary splenic DLBCL; however, the effectiveness of various treatments for it has not been adequately described previously. The purpose of this study was to compare the effectiveness of various treatments on survival time in primary splenic DLBCL. A total of 347 patients with primary splenic DLBCL were enrolled in The Surveillance, Epidemiology, and End Results (SEER) database. These patients were subsequently divided into four subgroups according to the treatment modalities: non-treatment group (patients who had not received chemotherapy, radiotherapy, or splenectomy, n=19), splenectomy group (patients who had received splenectomy only, n=71), chemotherapy group (patients who had received chemotherapy only, n=95), and the splenectomy combined with chemotherapy group (patients who had received splenectomy and chemotherapy, n=162). The overall survival (OS) and cancer specific survival (CSS) of four treatment groups were evaluated. Compared to the splenectomy group and the non-treatment group, the OS and CSS of the splenectomy combined with chemotherapy group was extremely significantly prolonged (P<0.01). Compared with the chemotherapy group, the OS and CSS of the splenectomy combined with chemotherapy group were longer, but there was no statistical difference (P>0.05). The Cox regression analysis showed that the treatment modality was identified as an independent prognostic factor for primary splenic DLBCL. The landmark analysis shows that the overall cumulative mortality risk was significantly lower in the splenectomy combined with chemotherapy group than in the chemotherapy group within 30 months (P<0.05), and the cancer-specific mortality risk was significantly lower in the splenectomy combined with chemotherapy group than in the chemotherapy group within 19 months (P<0.05). Splenectomy combined with chemotherapy may be the most effective treatment modality for primary splenic DLBCL.

Comprehensive Review of Acute Pancreatitis Pain Syndrome

Pancreatitis is a condition that causes inflammation in the pancreas, an organ located behind the stomach. This condition often presents as neuropathic, inflammatory, and/or visceral pain. Acute pancreatitis is typically characterized by sudden and severe abdominal pain, often in the upper right part of the abdomen. The pain from pancreatitis can be caused by different mechanisms, such as abnormal activation of pancreatic zymogens or NF-κB mediated inflammation in the pancreas. The treatment of pancreatitis depends on its type, severity, and underlying cause. Hospitalization and medications are typically necessary, while in others, surgery may be required. Proper management of pancreatitis is essential, as it can help reduce the risk of complications and improve the patient’s quality of life. The literature on pancreatitis pain management evaluates systematic approaches and the effectiveness of various treatments, such as lidocaine, opioid agonists, ketamine, magnesium, endoscopic methods, spinal cord stimulation, and other novel treatments present opportunities for exploration in pancreatitis pain management.