Effects Of Thermal Variations Research Articles

Environmental Temperature Variation Affects Brain Lipid Composition in Adult Zebrafish (Danio rerio).

This study delves deeper into the impact of environmental temperature variations on the nervous system in teleost fish. Previous research has demonstrated that exposing adult zebrafish (Danio rerio) to 18 °C and 34 °C for 4 or 21 days induces behavioural changes compared to fish kept at a control temperature of 26 °C, suggesting alterations in the nervous system. Subsequent studies revealed that these temperature conditions also modify brain protein expression, indicating potential neurotoxic effects. The primary aim of this work was to investigate the effects of prolonged exposure (21 days) to 18 °C or 34 °C on the brain lipidomes of adult zebrafish compared to a control temperature. Analysis of the brain lipidome highlighted significant alteration in the relative abundances of specific lipid molecules at 18 °C and 34 °C, confirming distinct effects induced by both tested temperatures. Exposure to 18 °C resulted in an increase in levels of phospholipids, such as phosphatidylethanolamine, alongside a general reduction in levels of sphingolipids, including sphingomyelin. Conversely, exposure to 34 °C produced more pronounced effects, with increases in levels of phosphatidylethanolamine and those of various sphingolipids such as ceramide, gangliosides, and sphingomyelin, alongside a reduction in levels of ether phospholipids, including lysophosphatidylethanolamine ether, phosphatidylethanolamine ether, and phosphatidylglycerol ether, as well as levels of glycolipids like monogalactosyldiacylglycerol. These results, when integrated with existing proteomic and behavioural data, offer new insights into the effects of thermal variations on the nervous system in teleost fish. Specifically, our proteomic and lipidomic findings suggest that elevated temperatures may disrupt mitochondrial function, increase neuronal susceptibility to oxidative stress and cytotoxicity, alter axonal myelination, impair nerve impulse transmission, hinder synapse function and neurotransmitter release, and potentially lead to increased neuronal death. These findings are particularly relevant in the fields of cell biology, neurobiology, and ecotoxicology, especially in the context of global warming.

Temperature and immune challenges modulate the transcription of genes of the ubiquitin and apoptosis pathways in two high-latitude Notothenioid fish across the Antarctic Polar Front.

Thermal variations due to global climate change are expected to modify the distributions of marine ectotherms, with potential pathogen translocations. This is of particular concern at high latitudes where cold-adapted stenothermal fish such as the Notothenioids occur. However, little is known about the combined effects of thermal fluctuations and immune challenges on the balance between cell damage and repair processes in these fish. The aim of this study was to determine the effect of thermal variation on specific genes involved in the ubiquitination and apoptosis pathways in two congeneric Notothenioid species, subjected to simulated bacterial and viral infections. Adult fish of Harpagifer bispinis and Harpagifer antarcticus were collected from Punta Arenas (Chile) and King George Island (Antarctica), respectively, and distributed as follows: injected with PBS (control), LPS (2.5mg/kg) or Poly I:C (2mg/kg) and then submitted to 2, 5 and 8°C. After 1week, samples of gills, liver and spleen were taken to evaluate the expression by real-time PCR of specific genes involved in ubiquitination (E3-ligase enzyme) and apoptosis (BAX and SMAC/DIABLO). Gene expression was tissue-dependent and increased with increasing temperature in the gills and liver while showing an opposite pattern in the spleen. Studying a pair of sister species that occur across the Antarctic Polar Front can help us understand the particular pressures of intertidal lifestyles and the effect of temperature in combination with biological stressors on cell damage and repair capacity in a changing environment.

236 Assessment of Early-Stage Thermal Manipulation on Broiler Chicken Muscle Satellite Cell Populations at Transfer and Hatch

Abstract Multi-stage hatching egg incubators are challenging to manage but still common throughout the US broiler industry. It is difficult to satisfy all embryonic temperature requirements in systems where late-stage embryos must be kept from overheating while ensuring early-stage embryos are not at sub-optimal temperatures. Our previous work showed improvements in performance and meat yield when incubation temperature was increased to 38.6 °C from embryonic day (ED) 4 to 11 as opposed to lowering it to 36.4 °C compared with a 37.5 °C control. The objective of this work was to assess the effect of thermal variation (TV) during early-stage incubation (ESI) on muscle satellite cell (SC) heterogeneity in broiler chicks at transfer and hatch to explore the mechanism responsible for increased meat yield in our previous study. Broiler breeder eggs (n = 2,160) were incubated at 37.5 °C from embryonic day (ED) 0 to 3. On ED 4, COLD incubator set points decreased to 36.4 °C, HOT incubator set points increased to 38.6 °C, and control incubators remained 37.5 °C (n = 2 incubators per treatment). On ED 11, all incubators were set to 37.5 °C until ED 18 when eggs were transferred to hatchers. At transfer (ED 18) and hatch (ED 21), pectoralis major (PM) and biceps femoris (BF) muscle samples were collected from 6 chicks per treatment. Samples were immunofluorescence stained to facilitate taxonomy of SC populations expressing the myogenic regulatory factors and SC markers, MyoD, MRF4, and Myf5, by fluorescence microscopy. Data were analyzed as a 1-way ANOVA with the GLIMMIX procedure of SAS. Means were separated at P ≤ 0.05 with the PDIFF option. Tendencies were declared when 0.0501 ≤ P ≤ 0.10. Chicks from COLD incubators had the greatest density of MyoD+:Myf5+ SC in their BF (P = 0.0261) and tended to have a greater density of MyoD+:MRF4+ SC in their PM muscle (P = 0.0562) compared with chicks from HOT incubators. On ED 21, chicks from HOT incubators had the greatest density of MyoD+:MRF4+:Myf5+ SC in their PM muscle (P = 0.0406). Temperature gradients as small as 1.1 °C during ESI altered BF SC populations in chicks at transfer and PM SC populations at both transfer and hatch. Overall, these results indicate that TV during ESI can influence SC population kinetics in both PM and BF muscles of modern, fast-growing, high-yielding broiler chicks at ED 18 and 21. Further investigation is needed to understand the longer-term effects on SC populations and the cell signaling pathways involved over time during the post-hatch grow out period to explain the meat yield responses observed in our previous work.

308 Effect of Thermal Variation During Late-Stage Incubation and Sex on Broiler Chicken Body Weight and Carcass Parts Uniformity

Abstract Low uniformity of broiler live body weight, carcass, and parts weights can reduce overall processing yields and profitability. The objective of this study was to evaluate the effects of thermal manipulation (TM) during late-stage incubation (LSI) and sex on the uniformity and coefficient of variation (CV) in high yielding broilers. Yield Plus ′ Ross 708 broiler breeder eggs (n = 2,160) were incubated at 37.5 °C from embryonic day (ED) 0 to 10. From ED 11 to 18, eggs were incubated at 1 of 3 air temperature set points: 37.5 °C (CTL), 36.4 °C (COLD), or 38.6 °C (HOT; n = 2 incubators per treatment). On ED 18, all eggs were transferred to baskets in hatchers set to 36.7 °C. Chicks were vent sexed, placed in floor pens blocked by incubation treatment and sex (n = 6 replicate pens of 30 birds per treatment), and fed a common diet. Broilers were individually weighed at d 9, 23, and 32, then, the 15 birds from each pen closest to the pen median BW were processed on d 33. The distance of each bird BW from the pen mean and median, TM treatment, sex, and whole flock BW over time, uniformity of BW (within 10% of the mean), and intra-pen CV were calculated. Data were analyzed as a 2-way ANOVA using the GLIMMIX procedure of SAS ver. 9.4 with TM treatment and broiler sex serving as the main effects. Means were separated at P ≤ 0.05 with the PDIFF option. On d 9, a TM by sex interaction was observed, and the BW of female CTL chicks was less variable from the pen mean compared with broilers hatched from COLD and females from HOT incubators (P ≤ 0.026). On d 9, sub-optimal incubation temperatures (HOT and COLD) during LSI resulted in higher individual female bird BW variation from the pen mean and median (P = 0.013). Incubating chicks in COLD incubators during LSI increased d-9 individual bird BW distance from the treatment, sex, and flock mean and median BW compared with CTL and HOT (P ≤ 0.037). In addition, BW uniformity within a pen was less in broilers hatched from COLD incubators than both CTL and HOT on d 9 (P = 0.033) and tended to be less on d 32 (P = 0.099). The inverse was observed for CV (P ≤ 0.040). Broilers hatched from COLD and HOT incubators had greater CV for carcass (P = 0.049) and wing (P = 0.028) as well as drumstick (P = 0.079) weights. These results indicate that sub-optimal LSI conditions can negatively influence uniformity of broiler live BW, carcass and parts weights and highlights the importance of achieving optimal incubation conditions.

237 Evaluation of Thermal Variation During Early-Stage Incubation on Broiler Chicken Pectoralis Major Muscle Satellite Cell Mitotic Activity and Heterogeneity at 28 Days Post-Hatch

Abstract Muscle satellite cells (SC) have an essential role in post-hatch skeletal muscle growth. To evaluate the effects of thermal variation (TV) during early-stage incubation (ESI) on Pectoralis major (PM) muscle SC heterogeneity of broiler chickens reared to 28 d post-hatch a randomized complete block design experiment with a 3 × 2 factorial treatment structure was conducted. Yield Plus × Ross 708 broiler breeder eggs (n = 2,160) ranging from 54 to 59 g were allotted equally by weight into 90-egg trays (n = 4 trays per incubator). All eggs were pre-warmed at 24 °C for 8 h and incubated at 37.5 °C from embryonic day (ED) 0 to 4. From ED 4 to 11, eggs were incubated at 1 of 3 temperatures (n = 2 incubators per TV treatment); 37.5 °C (CTL), 36.4 °C (COLD), or 38.6 °C (HOT). On ED 11, all incubators were returned to the CTL (37.5 °C) set point until ED 18. On ED 18, all eggs were transferred to baskets in hatchers set to 36.7 °C. After 514 h of incubation, all chicks were removed simultaneously, vent sexed, and weighed. Chicks were randomly allotted to floor pens (6 pens per treatment per sex) blocked by location and reared on a common diet. On d 28, birds (n = 10 per TV x sex treatment) were injected with bromodeoxyuridine (BrdU) before PM sample collection. Cryosections were immunofluorescence stained to facilitate taxonomy of SC populations expressing the myogenic regulatory factors Pax7 and MyoD as well as the proliferation marker BrdU by fluorescence microscopy. Data were analyzed as a 2-way ANOVA using SAS PROC GLIMMIX with TV treatment and broiler sex as main effects. Means were separated with the PDIFF option at P ≤ 0.05. Tendencies were declared when 0.0501 ≤ P ≤ 0.10. A TV × sex interaction was observed only for the MyoD+: Pax7+ SC density (P = 0.0232); however, none of the pairwise mean comparisons were significant at P < 0.05. Though CTL male and COLD female chicks had twice the density of MyoD+:Pax7+ SC as CTL females (P = 0.0535) and HOT males (P = 0.0645), respectively. There is no clear explanation for this observation. There were no interactive or main effects on density of MyoD+, Pax7+, MyoD+:BrdU+, Pax7+:BrdU+, MyoD+:Pax7+:BrdU+ SC (P ≥ 0.1258) or nuclear density (P = 0.1017) in 28-d-old broilers. These data suggest that if TV during ESI impacts SC myogenic regulatory factor heterogeneity and mitotic activity in broilers, the effects have concluded by d 28 post-hatch in commercial broilers. Further exploration of earlier time points will be necessary to determine whether the previously observed effects of TV during ESI on growth performance and meat yield are SC-mediated post-hatch.

Numerical study of the FRP-concrete bond behavior under thermal variations

Abstract In a context where daily and seasonal temperature changes or potential fire exposure can affect the mechanical response of structures strengthened with fiber-reinforced polymer (FRP) composites during their life cycle, the present work studies the bond behavior of FRP laminates glued to concrete substrates under a thermal variation. The problem is tackled computationally by means of a contact algorithm capable of handling both the normal and tangential cohesive responses, accounting for the effect of thermal variations on the interfacial strength and softening parameters, which defines the failure surface and post cracking response of the selected specimen. A parametric investigation is performed systematically to check for the effect of thermo-mechanical adhesive and geometrical properties on the debonding load of the FRP-to-concrete structural system. The computational results are successfully validated against some theoretical predictions from literature, which could serve as potential benchmarks for developing further thermo-mechanical adhesive models, even in a coupled sense, for other reinforcement-to-substrate systems, useful for design purposes in many engineering applications.

Thermal analysis of natural fiber reinforced composites

Natural fiber composite materials grabs attention in modern industrial world. Evaluation of mechanical and thermal properties of composite requires lot of experimentation. In this paper an attempt is made to predict mechanical and thermal properties of natural fiber composite from mathematical relations. Different natural fibers and epoxy matrix were considered for the property prediction. Mechanical and thermal properties of composites were predicted using Halpin-Tsai and Chamis model respectively. Most common natural composite in traditional boat construction is wood. Here NFRP material is used as an alternative to wood. Stress concentration occurs on marine structures due to openings provided for electrical and plumping lines. In order to simulate the structural stability with NFRP material, a rectangular plate is selected for the study. The predicted properties used to study effect of thermal variation of plate with hole. Finite element model was developed to study the stress concentration over different hole diameter. L/D ratio is selected as the parameter for the study. L/D ratio versus stress plot revealed that structure is unsafe if the ratio is above 6.

Effects of Thermal Variations on the Tensile Behavior of FRCM Strengthening Systems

Effects of Thermal Variations on the Tensile Behavior of FRCM Strengthening Systems

Weak effects on growth and cannibalism under fluctuating temperatures in damselfly larvae

The Earth’s climate is changing with a trend towards higher mean temperatures and increased temperature fluctuations. Little attention has been paid to the effects of thermal variation on competition within species. Understanding the temperature-dependence of competition is important since it might affect dynamics within and between populations. In a laboratory experiment we investigated the effects of thermal variation on growth and cannibalism in larvae of a damselfly. The temperature treatments included three amplitudes between 20 and 26 °C with an average of 23 °C, and a constant control at 23 °C. Larvae were also raised at five constant temperatures for an estimation of the thermal performance curve, which showed that the thermal optimum for growth was 26.9 °C. Cannibalism was significantly positively correlated with initial body size variance. There was neither a difference among the temperature variation treatments, nor between the constant and the variation treatments in growth and cannibalism. Hence, positive and negative effects of temperature variation within the linear range of a species thermal performance curve might cancel each other out. Since our study mimicked natural temperature conditions, we suggest that the increase in temperature variation predicted by climate models will not necessarily differ from the effects without an increase in variation.

Harvesters (Arachnida: Opiliones) mate more at cooler temperatures, but the effect of temperature on mating varies across years

AbstractTemperature impacts a wide range of mating behaviors, particularly in ectothermic organisms that tend to have body temperatures similar to ambient thermal conditions. Here, we test the effects of thermal variation on precopulatory and copulatory behavior in the harvester Leiobunum politum Weed 1889, which belongs to the group commonly known as daddy longlegs. We ran single choice mating trials across temperatures commonly experienced in the field during the mating season (18–34°C) for 2 years. We tested how temperature affected the likelihood to move, attempt to mate, and successfully mate, as well as the duration of copulation. Mating was highest at low to intermediate temperatures, and the temperature at which peak mating rates occurred varied across years. The wide range of temperatures across which L. politum is found to mate reflects thermal variability in the field and the flexibility in mating behavior in this fascinating animal.

Thermal Stability Analysis of Surface Wave Assisted Bio-Photonic Sensor

In this paper, the thermal stability of a Bloch Surface Wave (BSW) assisted bio-photonic sensor is investigated. The structural analysis is carried out using the transfer matrix method (TMM). The design comprises a truncated one-dimensional photonic crystal (1D-PhC) structure along with a defective top layer. The structural parameters are optimized to excite a BSW at the top interface for an operating wavelength of 632.8 nm. The mode confinement is confirmed by using wavelength interrogation, angular interrogation and surface electric field profile. Further, the effect of thermal variation on BSW excitation angle and sensitivity is carried out. The analysis shows the average variations in excitation angle and sensitivity of about −0.00096 degree/°C and 0.01046 (degree/RIU)/°C, respectively. Additionally, the analysis is also extended towards different lower wavelengths of 400 nm and 550 nm, which provides average variations in the excitation angles of about −0.0027 degree/°C, and 0.0016 degree/°C. This shows that the structural sensitivity response is more thermally stable at the lower wavelength range. Thus, showing its potential applications in designing thermally stable bio-photonic sensors.

Rates of warming impact oxidative stress in zebrafish (Danio rerio).

Potentially negative effects of thermal variation on physiological functions may be modulated by compensatory responses, but their efficacy depends on the time scale of phenotypic adjustment relative to the rate of temperature change. Increasing temperatures in particular can affect mitochondrial bioenergetics and rates of reactive oxygen species (ROS) production. Our aim was to test whether different rates of temperature increase affect mitochondrial bioenergetics and modulate oxidative stress. We exposed zebrafish (Danio rerio) to warming from 20°C to 28°C over 3, 6, 24 or 48 h, and compared these with a control group that was kept at constant 20°C. Fish exposed to the fastest (3 h) and slowest (48 h) rates of warming had significantly higher rates of H2O2 production relative to the control treatment, and the proportion of O2 converted to H2O2 (H2O2/O2 ratio) was significantly greater in these groups. However, ROS production was not paralleled by differences in mitochondrial substrate oxidation rates, leak respiration rates or coupling (respiratory control ratios). Increased rates of ROS production did not lead to damage of proteins or membranes, which may be explained by a moderate increase in catalase activity at the fastest, but not the slowest, rate of warming. The increase in ROS production at the slowest rate of warming indicates that even seemingly benign environments may be stressful. Understanding how animals respond to different rates of temperature change is important, because the rate determines the time period for phenotypic adjustments and it also alters the environmental thermal signal that triggers compensatory pathways.

Study of the Contact Thermal Compression Behavior of Copper using Scanning Contact Potentiometry Method and Finite Element Analysis

The issue of tracking residual stresses initiation in welding copper under various affected conditions is essential in increasing safety through improving the welding quality in particular, in the nuclear industry. This study investigated the behavior of welded copper numerically and experimentally under contact-heating compression test with constant clamping force. The scanning contact potentiometry (SCP) method was used to track the initiation and development of residual stresses within the weld zone. Furthermore, the finite element analysis (FEA) method was used to simulate and study the effect of thermal variations, with a constant compressive force, on mechanical factors that contribute to residual stresses formation within the weld zone. Additionally, scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDS) were used to get further information about the topography and composition of the specimen's surface. SCP results show that residual stress initiated from within the volume after 200 °C, and with further oxidation, its formation began on the surface after 250 °C. Using the relationship between maximum values of linearized von Mises stresses and maximum values of ADS at high SLS = 4.523, it was found that residual stresses generation began after 150 °C within the weld zone, and thermal stresses linearly increase with temperature due to further thermal expansion, which is associated with variation in linearized von Mises stresses and ADS maximum values. Comparison between potentiograms after 300 °C and FEA results have shown that the distribution of von Mises stress, normal stress, and total deformation are matching those in the distribution of ADS and are localized within the weld zone.
 HIGHLIGHTS
 
 The behavior of welded copper under compression at medium-low temperature range
 Residual stresses detection at an early stage localized within weldment zone
 Residual stresses increase with increased oxidation at copper surface
 Matched the SCP Experimental and FEA simulation results
 
 GRAPHICAL ABSTRACT

Vibration analysis of an axially functionally graded material non-prismatic beam under axial thermal variation in humid environment

The vibration analysis of an axially functionally graded material non-prismatic Timoshenko beam under axial thermal variation in humid environment is carried out using the harmonic differential quadrature method. In this modeling, the length and width of the beam remains constant whereas thickness of the beam is linearly varied along the axis of the beam. The material properties are temperature dependent and are assumed to be varied continuously along the axial direction according to power law distribution. Three types of temperature variations are considered in this study, that is, uniform temperature rise, linear temperature rise, and non-linear temperature rise. The temperature of the beam remains constant under uniform temperature rise condition and it is varied linearly and nonlinearly along the length of beam for rest of the conditions. The beam is subjected to uniform moisture concentration to impose humidity. Hamiltonian’s approach is used to derive the governing equations of motion. The resultant governing equations are then solved using the harmonic differential quadrature method to obtain the natural frequencies of the axially functionally graded material non-prismatic beam. The results obtained using the harmonic differential quadrature method are compared with results obtained for special cases. The effects of thermal variation, humidity, non-homogeneity parameter, and end conditions on natural frequencies of the non-prismatic beam are reported.

Investigation of Mechanical and Microstructural Properties of Fiber-Reinforced Geopolymer Concrete with GGBFS and Metakaolin: Novel Raw Material for Geopolymerisation

In modern days, one or the way, development of infrastructure plays a major role that decides upon the matrix of our lifestyle. The development of geopolymer concrete (GPC) is an important step towards the production of eco-friendly concrete. The effective usage of fiber content can reduce environmental pollution issues. An attempt was made to study the effect of the blend of metakaolin (MK) and ground granulated blast furnace slag (GGBFS) with Gujcon CFR fiber as secondary reinforcement was added as source material on the performance of GPC to prevent the effects of thermal variation. Fine aggregate, coarse aggregate, sodium hydroxide, nano-silicon dioxide (nano-SiO2), and cement have been used in the present work to make geopolymer mortar (GPM). GGBFS and Gujcon CFR fiber have been replaced with metakaolin an interval of 25%. The ratio of liquid to solid is 0.45 to 0.55, with a rise of 0.05. The samples are tested for split tensile and compression. To identify the surface morphology and phases SEM, XRD and EDAX have been analyzed. It is evident from the result that an equal proportion of source material with liquid to binder ratio 0.5 has yielded the highest mechanical properties.

Methodology for selection of optimum interference fit for enhancing performance of external gear pumps

External gear pumps are the heart of mobile hydraulic equipment in the mining sectors where it requires precise control of work attachment functions, lubrication of transmissions and oil supply to steering control units. The external gear pumps encounter many tribological phenomena such as lubrication and wear of gear teeth, an inner profile of casing, wear plates, and press fitting of journal bearings. The major challenge involved during the design phase is the optimum selection of required interference dimensions between the shaft plain bushings and its housings such as bracket and covers. Though the analytical calculations provide some rough initial sizes, it is a time consuming and tedious process when it comes to different material combinations, non-uniform and unsymmetrical sections of brackets and housings. Also, it is difficult to include the effect of thermal variations and different surface finish conditions of mating surfaces in analytical calculations. Optimum interference dimensions are essential to avoid premature failure of the Pumps due to dislodging of the bushings. In the current study, the required initial interference fit dimensions are computed based on an analytical method for different materials combinations. Further, the effect of the surface finish of mating surfaces and thermal expansion of materials are studied using KISSsoft simulation. The validation of the same has been carried out using an experimental set up with different material and assembly conditions as a comparative study to find the optimum dimensions.

How will the key marine calcifier <i>Emiliania huxleyi</i> respond to a warmer and more thermally variable ocean?

Abstract. Global warming will be combined with predicted increases in thermal variability in the future surface ocean, but how temperature dynamics will affect phytoplankton biology and biogeochemistry is largely unknown. Here, we examine the responses of the globally important marine coccolithophore Emiliania huxleyi to thermal variations at two frequencies (1 d and 2 d) at low (18.5 ∘C) and high (25.5 ∘C) mean temperatures. Elevated temperature and thermal variation decreased growth, calcification and physiological rates, both individually and interactively. The 1 d thermal variation frequencies were less inhibitory than 2 d variations under high temperatures, indicating that high-frequency thermal fluctuations may reduce heat-induced mortality and mitigate some impacts of extreme high-temperature events. Cellular elemental composition and calcification was significantly affected by both thermal variation treatments relative to each other and to the constant temperature controls. The negative effects of thermal variation on E. huxleyi growth rate and physiology are especially pronounced at high temperatures. These responses of the key marine calcifier E. huxleyi to warmer, more variable temperature regimes have potentially large implications for ocean productivity and marine biogeochemical cycles under a future changing climate.

Hot Rocks and Not-So-Hot Rocks on the Seashore: Patterns and Body-Size Dependent Consequences of Microclimatic Variation in Intertidal Zone Boulder Habitat.

SynopsisMicroclimatic variation has emerged as an important driver of many ecological and evolutionary processes. Nonetheless, fine-scale temperature data are still rare in most habitats, limiting our ability to understand the consequences of microclimatic variation under current and future conditions. We measured fine-scale thermal variation in a common, species-rich, but rarely studied habitat with respect to temperature: the airspaces under rocks on intertidal zone boulder shores. The effects of thermal variation were investigated using physiological, behavioral, and demographic responses of the porcelain crab Petrolisthes cinctipes. Habitat temperatures were measured at fine spatial and temporal resolution over 18 months, producing 424,426 temperature records. Microclimatic variation increased with increasing intertidal elevation, particularly with respect to heat extremes. However, mean temperatures were similar across the entire intertidal zone. Overheating risk for P. cinctipes increases with intertidal elevation but is size dependent, as large animals are more heat sensitive than small animals. Still, microclimatic variation high in the intertidal zone provided thermal refugia even under the warmest conditions. Size-dependent thermal responses predicted that large crabs should be rare high in the intertidal zone, which was supported by demographic data. Furthermore, simulations parameterized by our microclimate and organismal data recapitulated demographic patterns. Therefore, interactions between microclimatic variation and size-dependent thermal responses may have significant ecological repercussions that warrant greater attention.

Performance in a variable world: using Jensen's inequality to scale up from individuals to populations.

Body temperature affects plants' and animals' performance, but these effects are complicated by thermal variation through time within an individual and variation through space among individuals in a population. This review and synthesis describes how the effects of thermal variation-in both time and space-can be estimated by applying a simple, nonlinear averaging scheme. The method is first applied to the temporal variation experienced by an individual, providing an estimate of the individual's average performance. The method is then applied to the scale-dependent thermal variation among individuals, which is modelled as a 1/f-noise phenomenon. For an individual, thermal variation reduces average performance, lowers the temperature of maximum performance (Topt ) and contracts the range of viable temperatures. Thermal variation among individuals similarly reduces performance and lowers Topt , but increases the viable range of average temperatures. These results must be viewed with caution, however, because they do not take into account the time-dependent interaction between body temperature and physiological plasticity. Quantifying these interactions is perhaps the largest challenge for ecological and conservation physiologists as they attempt to predict the effects of climate change.

Hindered and constrained: limited potential for thermal adaptation in post-metamorphic and adult Rana temporaria along elevational gradients.

Adaptation to warming climates could counteract the effects of global warming. Thus, understanding how species cope with contrasting climates may inform us about their potential for thermal adaptation and which processes may hamper that ability (e.g. evolutionary trade-offs, phenology or behavioural thermoregulation). In addition to temperature, time constraints may also exert important selective pressures. Here, we compare the thermal sensitivity of locomotion of metamorphic and adult European common frogs (Rana temporaria) originating from populations along an elevational gradient. We employed the template mode of variation (TMV) analysis to decompose the thermal sensitivity of locomotion and explore the existence of trade-offs ('hotter is better' and 'specialist-generalist') and the degree of local adaptation. To that end, we studied the relationship between TMV parameters and local environmental conditions. Further, we compared preferred temperatures to assess whether behavioural thermoregulation could dampen the effects of thermal variation, reducing the intensity of selection and limiting thermal adaptation (i.e. 'Bogert effect'). We suggest that behavioural thermoregulation has promoted the conservatism of thermal sensitivity in R.temporaria. Yet, we observed a trend towards narrower thermal niches shifted towards warmer temperature in populations with severe temporal constraints, conforming to the 'generalist-specialist' trade-off. Apparently, this enables time-constrained populations - especially in the case of metamorphs - to effectively exploit resources during the warmest periods. The limited potential of R.temporaria for thermal adaptation suggests that forecasts of global warming should incorporate thermoregulation and explore its potential to buffer species from rising temperatures.