Greening cities is a key solution to improve the urban microclimate and mitigate the impact of climate change. However, the effect of tree planting on pollutant dispersion in streets is still a debated topic. To shed light on this issue, we present a wind-tunnel experiment aimed at investigating the effect of trees on street canyon ventilation. An idealized urban district was simulated by an array of blocks, and two rows of model trees were arranged at the sides of a street canyon oriented perpendicularly with respect to the wind direction. Reduced scale trees were chosen to mimic a realistic shape and aerodynamic behaviour. Three different spacings between the trees were considered. A passive scalar was injected from a line source placed at ground level and concentration measurements were performed in the whole canyon. Results show that the presence of trees alters the concentration pattern in the street with a progressive shift from a nearly two-dimensional to a three-dimensional field depending on tree density. Despite the significant change of the concentration field induced by trees, the average level of pollution in the street, and thus the overall ventilation efficiency, does not show a specific trend with the density of trees.