A three-point eccentric end-notched flexure (3EENF) test was conducted on Sitka spruce to characterize the Mode II resistance curve (R-curve). To extend the range for stabilized crack growth, grooves were cut along both side surfaces of the sample to reduce the region around the neutral axis. The R-curve was determined by varying the initial crack lengths, and fracture toughness values were measured at both the onset and during growth. Additionally, the effect of side grooves on stabilizing crack growth in the 3EENF test was investigated by comparing numerical simulation results with those from four-point end-notched flexure (4ENF) tests. The grooves effectively stabilized crack growth, and the stabilization range was equivalent to that of the 4ENF test when the initial crack length was appropriately determined. The grooved sample exhibited behavior indicative of a longer crack than the actual length.