Effect Of Peel Extract Research Articles

Insight into Photocatalytic Degradation of Amoxicillin by Biofabricated Granular Zinc Oxide Nanoparticle: Mechanism, Optimization and Toxicity Evaluation

Amoxicillin trihydrate (AMX) is an antibiotic drug and their metabolism in aqueous phase produce adverse effects on environmental standards and human health. Present research discloses the remediation of AMX antibiotic by photocatalytic degradation using fabricated zinc oxide nanoparticles (ZnONPs). Zinc oxide behaves as an excellent n-type semiconducting material for its high band gap (~ 3.37 eV). In this investigation, the ZnONPs were synthesized biologically using banana peels extract as both reducing and capping agent. The effects of peel extract, precursor concentration and pH on ZnONPs synthesis were investigated and optimized using response surface methodology (RSM). Moreover, the synthesised nanoparticle was characterised by UV–Vis, FESEM-EDX, TEM, XRD, dynamic light scattering (DLS) and FTIR. The characteristics properties give confirmation about the spherical shape, crystalline nature of the synthesized nanoparticle. Results revealed that over 90% of AMX can be photocatalytically degraded in 120 min irradiation under UV-B irradiation. The rate of degradation of AMX was better agreed with the pseudo-second-order kinetics. The thermodynamics of the antibiotic degradation revealed that reactions were exothermic and spontaneous nature. Additionally, effective efficacy of antibiotic residues have been tested against E. coli bacteria and poor efficiency against bacteria was recorded. Finally, it can be suggested that the ZnONPs could be an effective semiconductor towards degradation of AMX from wastewater.

Protective effects of peel extracts of Irvingia wombolu on metabolic disorders in streptozotocin-induced diabetic rats

BackgroundDiabetes is widely recognized as associated with several structural and functional liver, kidney, and heart abnormalities. Therefore, the present study was conducted to evaluate the protective effect of peel extracts of Irvingia wombolu against diabetes complications.MethodsDiabetes was induced by intravenous administration of streptozotocin (STZ) (50 mg/kg) through the right jugular vein on rats and animals with blood glucose values of at least 250 mg/dl received orally aqueous extract of peel (AEP), hydroethanolic extract of peel (HEP), tolbutamide and DMSO 10%. Their effects on the concentration of blood glucose, total cholesterol, HDL-C, LDL-C, triglycerides, malondialdehyde (MDA) and activities of catalase (CAT), reduced glutathione (GSH), superoxide dismutase (SOD), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in diabetic and normal rats were investigated using standard methods.ResultsTwenty-one days of treatment with AEP and HEP at a dose of 400 mg/kg significantly (P < 0,05) reduced the fasting blood glucose to a point of reaching normal value. The antihyperlipidemic assessment of extracts revealed a significant (P < 0,05) decrease in total cholesterol, triglycerides, LDL levels, and a significant (P < 0,05) increase in HDL level in the plasma of treated diabetic rats. Furthermore, plasma biomarkers of liver and kidney dysfunction were significantly reduced in treated diabetic rats. We also observed increased activities of catalase, SOD, and reduced glutathione in diabetic treated rats.ConclusionThe present findings suggest that AEP and HEP have a protective effect on liver, kidney, and heart in experimental diabetic rats which can be beneficial in the management of diabetes and its complications.

Effects of Peel Extract from Citrus reticulata and Hesperidin, A Citrus Flavonoid, on Macrophage Cell Line

The extract of Citrus reticulata has been studied for its biological activities, due to its citrus flavonoid content. The extract and its flavonoid compounds exhibit growth inhibition property in several cancer cell lines and in vivo models. Conversely, the extract can also induce cell proliferation and angiogenesis, and shows estrogenic effects by in vitro and in vivo. Because of the contrasting effects that depend on the concentration or dosage, the precise action of the extract and its flavonoids need to be elucidated in various cell types. The objective of this study is to evaluate the effect of Citrus reticulata peel extract (Citrus extract) and hesperidin, a citrus flavonoid, on the modulation of cell proliferation in the RAW 264.7 macrophage cell line. Cell viability under Citrus extract or hesperidin treatment was assessed by using the MTT assay. The expression of interleukin-10 (IL-10), an anti-inflammatory cytokine, modulated by Citrus extract was also examined by immunostaining. Low concentrations of Citrus extract at 1 and 100μg/mL were able to induce cell proliferation, although in significant, as shown by cell viability of 138 and 114%, respectively. At higher concentrations of 500, 750, and 1000μg/mL, Citrus extract decreased cell viability significantly by up to 64, 46, and 36%, respectively. Accordingly, hesperidin at low (3.1μg/mL −61.1μg/mL) concentration increased cell viability significantly by up to 116-136% where as high (152.6203μg/mL–305.3203μg/mL) concentration reduced cell viability significantly by up to 10-61%. The value of the 50% inhibitory concentration (IC50) of Citrus extract was more than three times higher (756μg/mL) than that of hesperidin (203μg/mL = 332μM). Additionally, 250μg/mL of Citrus extract was able to induce IL-10 expression compared to control. These results demonstrated that Citrus extract and hesperidin exerted a biphasic effect on macrophage cells. The future development of Citrus extract as a co-chemotherapeutic, anticancer, or immunomodulatory agent should include careful consideration of its biphasic effect on each cell type

Novel phenotypes related to the breeding of purple-fruited tomatoes and effect of peel extracts on human cancer cell proliferation

The production of anthocyanins in the tomato (Solanum lycopersicum L.) fruit is normally absent or poor, but a number of mutants or introgression lines are known to increase anthocyanin levels in vegetative and reproductive tissues. Through conventional breeding, a genetic combination was obtained with the remarkable phenotype of a deep purple fruit pigmentation, due to an accumulation of anthocyanins on the peel. Such a genotype was named Sun Black (SB) as a consequence of its sensitivity to light induction. When characterized for morpho-agronomic traits, SB plants showed increased fertility. Purple fruits displayed an arrangement of the epicarp cells different from normal tomatoes, a feature that could account for different mechanical properties and shelf-life potential. The SB genotype and, to a lesser extent, its single mutant parents showed the capacity to accumulate anthocyanins in the seedling root when grown under light. This phenotype, which was greatly improved by the addition of sucrose to the germination medium, proved to be useful as selection index and gave new insights for in vitro production of anthocyanin extracts. To assess the nutraceutical potential of purple tomatoes, we tested the activity of SB skin extracts on the proliferation of two human cancer cells lines. Cell proliferation was significantly inhibited by SB extract in a dose-dependent manner. When the bioactivity of SB extracts was compared with that of other anthocyanin-containing fruits or vegetables, a significant "Extract*Line" interaction was evidenced, suggesting a crucial role for the extract composition in terms of anthocyanidins and other eventual cell growth-inhibiting compounds.

Mango (Mangifera indica L.) peel extract fractions from different cultivars differentially affect lipid accumulation in 3T3-L1 adipocyte cells

Plant phytochemicals are increasingly recognised as sources of bioactive molecules which may have potential benefit in many health conditions. In mangoes, peel extracts from different cultivars exhibit varying effects on adipogenesis in the 3T3-L1 adipocyte cell line. In this study, the effects of preparative HPLC fractions of methanol peel extracts from Irwin, Nam Doc Mai and Kensington Pride mangoes were evaluated. Fraction 1 contained the most hydrophilic components while subsequent fractions contained increasingly more hydrophobic components. High content imaging was used to assess mango peel fraction effects on lipid accumulation, nuclei count and nuclear area in differentiating 3T3-L1 cells. For all three mango cultivars, the more hydrophilic peel fractions 1-3 inhibited lipid accumulation with greater potency than the more hydrophobic peel fractions 4. For all three cultivars, the more lipophilic fraction 4 had concentrations that enhanced lipid accumulation greater than fractions 1-3 as assessed by lipid droplet integrated intensity. The potency of this fraction 4 varied significantly between cultivars. Using mass spectrometry, five long chain free fatty acids were detected in fraction 4; these were not present in any other peel extract fractions. Total levels varied between cultivars, with Irwin fraction 4 containing the highest levels of these free fatty acids. Lipophilic components appear to be responsible for the lipid accumulation promoting effects of some mango extracts and are the likely cause of the diverse effects of peel extracts from different mango cultivars on lipid accumulation.

Growth inhibitory effect of peel extract from Citrus junos

Extract from yuzu fruit peel (Citrus junos Sieb. ex Tanaka) strongly suppressed the germination of lettuce seeds while that from the peel of other citrus fruits such as navel orange (C. sinensis) and lemon (C. limon Burm. f.) had very little or no effect. The highest inhibitory activity was located in the peel followed by the segment but no significant activity was found in the seed extract of yuzu fruit. The effect of yuzu peel extract was tested on a variety of major crops including 38 crop species. Germination of seeds of tomato (Lycopersicon esculentum Mill.), celery (Apium graveolens L. var. dulce) and watercress (Nasturtium officinale R. Br.) was completely blocked by 27.8 mg dry peel equivalent ml−1 of yuzu peel extract, while that of cucumber (Cucumis sativus L.) and pumpkin (Cucurbita moschata [Duch. ex Lam.] Duch. ex Poir.) seeds was not affected at this concentration, showing a broad variation in the sensitivity of crop seeds to the extract. The effectiveness of yuzu peel was also tested on some harmful weeds. The extract inhibited the elongation of both radicle and hypocotyl in summer weed quinoa (Chenopodium quinoa) grown on agar plates. Yuzu peel powder added to the soil was also effective in suppressing the growth of C. quinoa, Sonchus oleraceus L. and Digitaria ciliaris, while it had little effect on the spring weed Alopecurus aequalis Sobol. A strong growth inhibitory activity of yuzu peel was found in the aqueous phase after solvent extraction and most of the activity was recovered in the neutral fraction that passed through cation and anion exchange resins.