Effect Of Ovalbumin Research Articles

The Effects of Ovalbumin on Proliferation, Migration, and Stemness Properties of Chemoresistant SW480 Colon Cancer Cells

Egg peptides are factors in the embryonic environment with many significant biological activities, such as anticancer activity. Therefore, the current study investigates the effect of egg ovalbumin (OVA) on survival, cell cycle, self-renewal ability, stemness properties, and migration in SW480 colon cancer cells and 5-fluorouracil (5FU) resistant subgroup. MTT test was performed to assess cell viability. Flow cytometry was employed to analyze the cell cycle. Clonogenic assay and spheroid formation were used to assess the effect of OVA on self-renewal and stemness properties. Wound healing assay and RT-PCR were performed to analyze migration and gene mRNA expression. We demonstrated that OVA (8 and 12 mg/ml) attenuated cell viability, induced cell-cycle arrest, inhibited colony formation, and non-significantly reduced spheroid formation and migration in both cell lines. Furthermore, OVA downregulated the expression of Nanog, c-Myc, and NDRG1 in both cells, suggesting a stemness and self-renewal attenuation by OVA. In conclusion, OVA exposure inhibited the 5FU-SW480 chemo-resistant subpopulation growth by inducing cell cycle arrest and diminishing self-renewal and partially stemness properties of colon cancer cells.

Development of carrot top powders using foam mat drying

Carrot tops are under-exploited greens despite having high nutritional value. The effect of ovalbumin and Emustab® concentration and whipping time on foam characteristics was evaluated, and 10% (w/w) of Emustab® and 10 min of whipping was selected for use. Foam mat drying was performed at 50–70°C, and effects of temperature on physicochemical properties, morphology, and centesimal composition of the powders were investigated. Midilli and Kucuk’s model best represented the drying kinetics (R2 = 0.994). Increasing temperature reduced drying time by more than three-fold and significantly (p < .05) affected the moisture content (6.15–11.82% d.b.), hygroscopicity (1.27–2.57%), rehydration ratio (3.30–3.77), and total color difference (14.07 < ΔE < 16.77) of the carrot tops’ powders. Powder at 50°C showed higher moisture content, lower hygroscopicity, and better rehydration rate. Drying at 70°C produced powders with lower ΔE and higher protein content. For all drying conditions, however, fresh carrot tops yielded stable and porous powders, promising for use in ready-to-cook foods. Practical applications Carrot tops are one of the most underutilized greens of appreciable nutritive value. The foam mat drying proved to be promising for the dehydration of these materials, being the carrot top powders extensively characterized to know its properties and variations caused by drying. The results obtained will benefit both researchers and industry professionals to design better drying processes and innovative products based on carrot tops.

Interaction of ovalbumin with lutein dipalmitate and their effects on the color stability of marigold lutein esters extracts

In this study, the interaction of ovalbumin with lutein dipalmitate and the effect of ovalbumin on marigold lutein esters extracts were investigated. Lutein dipalmitate quenched the fluorescence of ovalbumin by static quenching. Binding and thermodynamic parameters proved that lutein dipalmitate bound to ovalbumin spontaneously by van der Waals force and hydrogen bond, and the complex stoichiometry was 1:1. Through three-dimensional fluorescence spectroscopy, Fourier transform infrared spectroscopy and circular dichroism experiments, the conformation of ovalbumin was unfolded, and alteration in the ovalbumin secondary structure induced by lutein dipalmitate was observed. The results of transmission electron microscopy and particle size revealed that there were spherical and nano-sized aggregates in the ovalbumin-lutein dipalmitate system, indicating the lutein dipalmitate not only could bind to ovalbumin at molecular level, but also promote the aggregation of ovalbumin. Additionally, the addition of ovalbumin had a positive effect on the stability of marigold lutein esters extracts.

Hepatic Cytochrome P450 Activity and Nitric Oxide Production During Multiple Ovalbumin Challenges.

Mast cell-mediated allergic diseases are a significant global health problem. Nitric oxide (NO) produced by acute type 1 allergies greatly suppresses hepatic cytochrome P450 (CYP) metabolism. A recent in vitro study demonstrated that repeated FcεRI-mediated activation intrinsically modulates mast cell function. We investigated the effect of ovalbumin (OVA) challenges on CYP activity and NO production under real immune responses. After repeated sensitization with OVA once a week, serum nitrate plus nitrite (NOx) and total plasma immunoglobulin E concentrations were measured using commercially available kits. Hepatic microsomal CYP-specific activities and protein expression were determined using typical substrates and by western blot, respectively. In the liver, the levels of inducible NO synthase (iNOS), F4/80, and c-kit mRNA were determined by real-time polymerase chain reaction. Hepatic total NOS activity was measured using a colorimetric assay kit. When mice received multiple OVA challenges, the 11th sensitization elevated NOx concentrations in serum and suppressed the activities of five major CYPs without altering protein expression levels. After the 7th, 11th, and 15th sensitizations, F4/80-positive Kupffer cell and hepatic c-kit-dependent mast cell mRNA levels were similar to those of the control. The 7th and 11th sensitizations increased hepatic iNOS mRNA expression to 15-fold and threefold above control levels, respectively, but did not enhance the total NOS activity in the liver. Multiple OVA challenges, unlike acute sensitization, greatly reduced serum NOx levels. The challenge-suppressed hepatic CYP metabolism was likely related to the increased serum NOx. Serum NOx may be an endogenous marker for CYP metabolism inhibition in type 1 allergic diseases.

Histological and Physiological Study of the Effect of Silver Nanoparticles and Omega-3 on Asthma of Male Mice Induced by Ovalbumin

In the current study ninety one of male mice weighting (25-30 g) aged (15-17) weeks at the animal house faculty of science / university of Kufa during the period from January 2017 to September 2017. This study included some physiological and histological criteria to evaluate the protective role of omega-3(2 and 3 mg/kg) and silver nanoparticles (5 and 10 mg/kg) against asthma that induced by ovalbumin. The animals experimental are divided into 16 groups (n= 6 mice per each group) for duration of one and two months. The results showed significant increase (p less than 0.05) in the leukocyte count (eosinophil, neutrophil, lymphocyte, monocyte) in asthma group as compared with control group .Also ,the results showed significant decrease (p less than 0.05) in the leukocyte count in the treated group of omega-3 and silver nanoparticles for both concentration as compared with asthma group .The result sowed significant increase(p less than 0.05) in the serum level of periostin and Galectin -3 and Interleukin -33 in asthma group as compared with control group. The histological study of lung tissue revealed that induced the tissue with ovalbumin caused necrosis, degeneration and increase of mucous in bronchiole as well as acute inflammation around bronchioles while the effect of ovalbumin in trachea tissue were sluphing, necrosis and degeneration around the epithelium of bronchioles.

12 Experimental study on the effect of Perilla seed in the rat model of asthma

Objectives To investigate the effects of Perilla seed in the rat model of bronchial asthma. Methods To observe the effect of ovalbumin (OVA) on the behavior of rats, NO and IL-6 levels in serum and in the lungs were measured using aluminium hydroxide sensitisation and an OVA aerosol inhalation challenge. Histopathological changes were examined to study the effects of Perilla consumption. Results The rat model of bronchial asthma was successfully established. Compared with model group, large, medium and small doses of Perilla seed significantly increased the weight of bronchial asthma rats (p Conclusions Perilla frutescens seed has a protective effect in the rat model of bronchial asthma. Acknowledgments The study was financially supported by the Collaborative Innovation Centre for Respiratory Disease Diagnosis and Treatment and Chinese Development of Henan Province National Natural Science Foundation of China (Grant No. XTZX2015023).

Effect of ovalbumin on the quality of gluten-free rice flour bread made with soymilk

The use of soymilk instead of water affects the rising of gluten-free rice flour bread into the shape of a muffin. However, when baking bread into a loaf, gas cells at the center of the crust, where thermal conduction is lowest, collect to form a hollow in the loaf. The goal of this study was to assess the components that affect the quality of gluten-free rice flour bread made with soymilk. Adding more than 1.25% of ovalbumin (OVA) to the rice flour prevented formation of this hollow. In addition, OVA levels greater than 2.5% helped the bread rise further through oven spring, which increased specific loaf volume. These improvements resulted from an increase in thermal coagulation of gas cell membranes and an increase in batter viscosity due to interactions between soy globulins and OVA that promoted starch granule aggregation. Thus, even though OVA levels greater than 2.5% increased the specific loaf volume, they also made the crust harder. As a result, the optimum OVA level is 1.25% of the rice flour.

THE EFFECT OF OVALBUMIN ON THE PROTEASE ACTIVITY

Chicken egg white is generally used in the industry because of its excellent effect to improve physical property of surimi products. The effects of egg white are believed to be derived from its inhibitory activity on surimi endogenous proteases which reduce gel forming ability. However, there are no detailed investigations about inhibitory effects of egg white on the proteases. The effect of ovalbumin (OVA), which is the main protein of egg white, on trypsin activity was investigated in the present study. N-OVA was purified from fresh chicken egg white by ammonium sulfate fractionation and anion-exchange chromatography (Q-sepharose). S-OVA and I-OVA were prepared by heating N-OVA solution at pH10, 55°C for 24h and at 97°C for 30min (pH was not controlled) respectively. To investigate the effect of OVA on the trypsin activity, casein-trypsin mixture solution was heated with or without OVAs at 40°C and the content of peptide generated from casein was measured. SDS-PAGE of casein was also conducted. The amount of peptide from casein was decreased in the presence of OVAs, regardless of OVA types. SDS-PAGE showed all types of OVA inhibited casein degradation. Those suggest that all types of OVA have inhibitory effect on the trypsin activity. Keywords : Surimi, Ovalbumin, Proteases, Egg white

Sensitizing effects of chronic exposure and acute inhalation of ovalbumin aerosol on pulmonary C fibers in rats

The effect of ovalbumin (Ova) sensitization on pulmonary C-fiber sensitivity was investigated. Brown-Norway rats were sensitized by intraperitoneal injection of Ova followed by aerosolized Ova three times per week for 3 wk. Control rats received the vehicle. At the end of the third week, single-unit fiber activities (FA) of pulmonary C fibers were recorded in anesthetized, artificially ventilated rats. Our results showed the following: 1) Ova sensitization induced airway inflammation (infiltration of eosinophils and neutrophils) and airway hyperresponsiveness in rats; 2) baseline FA in sensitized rats was significantly higher than that in control ones; 3) similarly, the pulmonary C-fiber response to right atrial injection of capsaicin was markedly higher in sensitized rats, which were significantly amplified after the acute Ova inhalation challenge; and 4) similar patterns, but smaller magnitudes of the differences in C-fiber responses to adenosine and lung inflation, were also found between sensitized and control rats. In conclusion, Ova sensitization elevated the baseline FA and excitability of pulmonary C fibers, and the hypersensitivity was further potentiated after the acute Ova inhalation challenge in sensitized rats. Chronic allergic inflammatory reactions in the airway probably contributed to the sensitizing effect on these lung afferents.

Thermodynamic investigation of salting effect of ovalbumin in various salts solutions

Calorimetric and volumetric investigations of ovalbumin salting by sodium chloride, lithium chloride, potassium chloride, lithium sulfate and ammonium sulfate buffered solutions in the range of concentrations of about 0.2–1.2 M of salts are reported. The evolution of enthalpies and apparent molar volumes vs. salts concentration was investigated using ITC MicroCal titration microcalorimeter and Anton Paar DMA 60/602 digital densimeter. It was found that the changes in enthalpies of salting and apparent molar volumes follow a similar trend. Conclusions about the ability of salts: to precipitate ovalbumin solution are presented.

The effect of ovalbumin and mannose-conjugated ovalbumin on the prevention of Salmonella adherence to the intestinal epithelium of chickens.

The effect of ovalbumin and mannose-conjugated ovalbumin on the prevention of Salmonella adherence to the intestinal epithelium of chickens.

Preformulation Studies on Freund's Incomplete Adjuvant Emulsion

Freund's Incomplete Adjuvant (FIA), which is used in vaccine therapy, is a water-in-oil emulsion delivery system consisting of an aqueous internal phase containing an antigenic protein dispersed in an external phase containing a mixture of mannide monooleate and light mineral oil. Preformulation studies are reported in this investigation for FIA emulsion. The preformulation studies included the determination of the critical micelle concentration (CMC) of the formulations investigated, the surface activity of mannide monooleate at the interface between the oil phase and the aqueous phase containing ovalbumin as the model antigenic protein, and the effect of ovalbumin on the surface activity at the interface. The influence of the concentration of mannide monooleate and/or ovalbumin on the interfacial tension between light mineral oil and either purified water or 0.9% w/v normal saline solution was measured by the DuNouy Ring Method at 25°C. The CMC was determined experimentally from the relationship between the concentration of the surface active agent in each formulation and the interfacial tension. The number of moles of the surface active agent per unit area at the interface (surface excess concentration) was calculated from the Gibbs' Adsorption equation. The results indicated that mannide monooleate was an effective surface active agent since the formulation containing only mannide monooleate provided the lowest magnitude of CMC. The presence of the surface active agent, mannide monooleate and/or ovalbumin, in the formulations studied reduced the interfacial tension between the two phases. The surface activity was influenced by the presence of an electrolyte (sodium chloride), a protein (ovalbumin), or mannide monooleate in the formulation. The presence of antigenic proteins in the aqueous phase of a water-in-oil emulsion influenced the effectiveness of a surface active agent in the formulation.

Effects of ovalbumin on the motility and fertilising ability of fowl spermatozoa stored for 24 H at 4 degrees C.

1. The effects of 0.1, 0.5, 1 and 3 g ovalbumin/100 ml diluent on spermatozoa stored for 24 h at 4 degrees C in TES diluent or BPSE diluent with or without seminal plasma were studied. 2. There was no effect of ovalbumin on the motility of spermatozoa stored in whole semen, except at an incorporation rate of 3 g/100 ml, when motility was reduced for spermatozoa stored in TES diluent. When sperm were stored in the absence of seminal plasma, ovalbumin stimulated motility but this effect was transitory. 3. The effects of ovalbumin on fertilisation rates were diluent- and concentration-dependent. Ovalbumin concentrations of 0.1 and 0.5 g/100 ml increased the fertilising ability of whole semen stored in TES diluent but 1 g/100 ml ovalbumin decreased it. However, 0.5 g ovalbumin/100 ml had no effect on the fertilising ability of spermatozoa stored in BPSE diluent, irrespective of the presence or absence of seminal plasma.