Effects Of Mechanical Perturbation Research Articles

Standing on unstable surface challenges postural control of tracking tasks and modulates neuromuscular adjustments specific to task complexity

Understanding the modulations of motor control in the presence of perturbations in task conditions of varying complexity is a key element towards the design of effective perturbation-based balance exercise programs. In this study we investigated the effect of mechanical perturbations, induced by an unstable surface, on muscle activation and visuo-postural coupling, when actively tracking target motion cues of different complexity. Four postural tasks following a visual oscillating target of varying target complexity (periodic-sinusoidal vs. chaotic-Lorenz) and surface (stable-floor vs. unstable-foam) were performed. The electromyographic activity of the main plantarflexor and dorsiflexor muscles was captured. The coupling between sway and target was assessed through spectral analysis and the system’s local dynamic stability through the short-term maximum Lyapunov exponent. We found that external perturbations increased local instability and deteriorated visuo-motor coupling. Visuo-motor deterioration was greater for the chaotic target, implying that the effect of the induced perturbations depends on target complexity. There was a modulation of the neuromotor system towards amplification of muscle activity and coactivation to compensate surface-related perturbations and to ensure robust motor control. Our findings provide evidence that, in the presence of perturbations, target complexity induces specific modulations in the neuromotor system while controlling balance and posture.

Mechanical perturbation control of cardiac alternans.

Cardiac alternans is a disturbance in heart rhythm that is linked to the onset of lethal cardiac arrhythmias. Mechanical perturbation control has been recently used to suppress alternans in cardiac tissue of relevant size. In this control strategy, cardiac tissue mechanics are perturbed via active tension generated by the heart's electrical activity, which alters the tissue's electric wave profile through mechanoelectric coupling. We analyze the effects of mechanical perturbation on the dynamics of a map model that couples the membrane voltage and active tension systems at the cellular level. Therefore, a two-dimensional iterative map of the heart beat-to-beat dynamics is introduced, and a stability analysis of the system of coupled maps is performed in the presence of a mechanical perturbation algorithm. To this end, a bidirectional coupling between the membrane voltage and active tension systems in a single cardiac cell is provided, and a discrete form of the proposed control algorithm, that can be incorporated in the coupled maps, is derived. In addition, a realistic electromechanical model of cardiac tissue is employed to explore the feasibility of suppressing alternans at cellular and tissue levels. Electrical activity is represented in two detailed ionic models, the Luo-Rudy 1 and the Fox models, while two active contractile tension models, namely a smooth variant of the Nash-Panfilov model and the Niederer-Hunter-Smith model, are used to represent mechanical activity in the heart. The Mooney-Rivlin passive elasticity model is employed to describe passive mechanical behavior of the myocardium.

Strain-shear coupling in bilayer MoS2

Layered materials such as graphite and transition metal dichalcogenides have extremely anisotropic mechanical properties owing to orders of magnitude difference between in-plane and out-of-plane interatomic interaction strengths. Although effects of mechanical perturbations on either intralayer or interlayer interactions have been extensively investigated, mutual correlations between them have rarely been addressed. Here, we show that layered materials have an inevitable coupling between in-plane uniaxial strain and interlayer shear. Because of this, the uniaxial in-plane strain induces an anomalous splitting of the degenerate interlayer shear phonon modes such that the split shear mode along the tensile strain is not softened but hardened contrary to the case of intralayer phonon modes. We confirm the effect by measuring Raman shifts of shear modes of bilayer MoS2 under strain. Moreover, by analyzing the splitting, we obtain an unexplored off-diagonal elastic constant, demonstrating that Raman spectroscopy can determine almost all mechanical constants of layered materials.

An elaborate data set on human gait and the effect of mechanical perturbations.

Here we share a rich gait data set collected from fifteen subjects walking at three speeds on an instrumented treadmill. Each trial consists of 120 s of normal walking and 480 s of walking while being longitudinally perturbed during each stance phase with pseudo-random fluctuations in the speed of the treadmill belt. A total of approximately 1.5 h of normal walking (>5000 gait cycles) and 6 h of perturbed walking (>20,000 gait cycles) is included in the data set. We provide full body marker trajectories and ground reaction loads in addition to a presentation of processed data that includes gait events, 2D joint angles, angular rates, and joint torques along with the open source software used for the computations. The protocol is described in detail and supported with additional elaborate meta data for each trial. This data can likely be useful for validating or generating mathematical models that are capable of simulating normal periodic gait and non-periodic, perturbed gaits.

Effect of mechanical perturbations on the formation of the welded joint

The results of investigation of the mechanical perturbations, caused by inaccuracies in the assembling of welded components and deviations in the operation of the mechanisms for displacement of the welding tool, are presented, and the methods and possibilities of preventing the consequences of welding under the effect of these perturbations are described.

Mechanics and Structure of Fibrin Networks Polymerized under Oscillatory Shear Perturbations

The mechanical properties of a blood clot are of crucial importance for its ability to stem the flow of blood at a site of vascular injury. These properties are largely determined by the underlying structural scaffold which forms during blood coagulation, a branched network of the biopolymer fibrin. Alterations in the structure of this network, such as changes in fiber density, fiber thickness or branching probability, strongly affect the mechanics of the network.The relationship between network architecture and bulk-level mechanics is commonly investigated using in vitro fibrin networks polymerized under static conditions. However, in the body, blood clots form under a highly dynamic mechanical environment: Nascent fibrin fibers are constantly exposed to the pulsatile shear flow of blood and the concurrent oscillatory dilation of the vessel walls. However, the effects of mechanical perturbations during polymerization on clot structure and the resultant mechanical properties remain unknown.Here, we polymerize fibrin networks while applying continuous oscillatory shear perturbations of varying strain amplitude. Despite these mechanical perturbations, fibrin can form rigid clots which exhibit a significantly later onset of the non-linear strain-stiffening response, a postponed rupture strain, and a lowered linear modulus compared to clots formed without perturbations. Up to perturbation amplitudes of 45% shear strain, the typical non-linear stiffness of these clots as well as their rupture stresses are of similar magnitude to those formed without perturbation. We show by confocal microscopy that these changes in the mechanical properties result from a formation of two architecturally distinct layers within the clot: one layer shows a highly bundled structure, while the other layer is virtually unaltered. This architectural adjustment may serve as a means for adapting blood clots to the mechanical loading conditions of the environment in which they form.

Power instability of singly resonant optical parametric oscillators: Theory and experiment

We present a theoretical model on the effects of mechanical perturbations on the output power instability of singly-resonant optical parametric oscillators (SR-OPOs). Numerical simulations are performed based on real experimental parameters associated with a SR-OPO designed in our laboratory, which uses periodically-poled LiNbO3 (PPLN) as the nonlinear crystal, where the results of the theoretical model are compared with the measurements. The out-coupled power instability is simulated for a wide range of input pump powers the SR-OPO oscillation threshold. From the results, maximum instability is found to occur at an input pump power of ~1.5 times above the OPO threshold. It is also shown theoretically that the idler instability is susceptible to variations in the cavity length caused by vibrations, with longer cavities capable of generating more stable output power. The validity of the theoretical model is verified experimentally by using a mechanical vibrator in order to vary the SR-OPO resonator length over one cavity mode spacing. It is found that at 1.62 times threshold, the out-coupled idler suffers maximum instability. The results of experimental measurements confirm good agreement with the theoretical model. An intracavity etalon is finally used to improve the idler output power by a factor of ~2.2 at an input pump power of 1.79 times oscillation threshold.

Diagnostic study and numerical simulation of the Bombay (India) Deluge

Bombay (latitude: 19.1°N, longitude: 72.9°E, altitude: 14 m asl) received unprecedented amount of rainfall, 94.4 cm in 24 h, from 26 to 27 Jul 2005. Observational and model simulation, based on Advanced Regional Prediction System, study of this extreme event is made. The thermodynamical assessment is made using vertical sounding in model simulation. Artificial thermal perturbation is introduced to initiate the instability for generation of vertical convection with assumption that convection is initiated due to the thermal forcings and neglecting the effect of mechanical perturbation (orographic forcings). Model could simulate the thermal nature of the storm as observed. Also, simulation shows the vigorous nature of thunderstorm with heavy downpour at the rate of 10 cm/h, which is close to the observed.

Effect of mechanical perturbation on the biomechanics, primary growth and secondary tissue development of inflorescence stems of Arabidopsis thaliana

Mechanical perturbation is known to inhibit elongation of the inflorescence stem of Arabidopsis thaliana. The phenomenon has been reported widely for both herbaceous and woody plants, and has implications for how plants adjust their size and form to survive in mechanically perturbed environments. While this response is an important aspect of the plant's architecture, little is known about how mechanical properties of the inflorescence stem are modified or how its primary and secondary tissues respond to mechanical perturbation. Plants of the Columbia-0 ecotype were exposed to controlled brushing treatments and then submitted to three-point bending tests to determine stem rigidity and stiffness. Contributions of different tissues to the inflorescence stem geometry were analysed. Perturbed plants showed little difference in stem diameter, were 50 % shorter, 75 % less rigid and 70 % less stiff than controls. Changes in mechanical properties were linked to significant changes in tissue geometry - size and position of the pith, lignified interfascicular tissue and cortex - as well as a reduction in density of lignified cells. Stem mechanical properties were modified by changes in primary tissues and thus differ from changes observed in most woody plants tested with indeterminate growth - even though a vascular cambium is present in the inflorescence axis. The study suggests that delayed development of key primary developmental features of the stem in this ecotype of Arabidopsis results in a 'short and flexible' rather than a 'short and rigid' strategy for maintaining upright axes in conditions of severe mechanical perturbation. The mechanism is comparable with more general phenomena in plants where changes in developmental rate can significantly affect the overall growth form of the plant in both ecological and evolutionary contexts.

Effects of mechanical perturbation at various times during incubation on egg survival, hatching and malformation rates in the rainbow trout Oncorhynchus mykiss, and the influence of post-ovulatory oocyte ageing

Effects of mechanical perturbation at various times during incubation on egg survival, hatching and malformation rates in the rainbow trout Oncorhynchus mykiss, and the influence of post-ovulatory oocyte ageing

The effect of mechanical perturbation during 3D reaching task on the kinematics and muscle function of the upper limb

The effect of mechanical perturbation during 3D reaching task on the kinematics and muscle function of the upper limb

Brushing effects on the growth and mechanical properties ofCorispermum mongolicumvary with water regime

High water availability and mechanical stress can induce opposite responses in plants. In arid areas of Northern China the occurrence of high wind and high water availability tend to be negatively correlated. Since turgor pressure is a determinant of the mechanical stability of annuals, it is hypothesised that the effects of mechanical perturbation (MP) on annuals may depend on soil water availability. To test this proposal, we conducted an experiment in which a pioneering annual Corispermum mongolicum was subjected to two levels of MP and water supply, and then determined its growth and mechanical traits. Brushing had no effect on plant height and total biomass, but stimulated leaf and branch production. Water supply affected plant height, basal diameter, total biomass and stem rigidity, but not leaf and branch number, root/shoot ratio or flexibility. With high water availability, brushing stimulated the production of stiffer stems (thicker and with a higher Young's modulus) and more roots relative to shoot mass, but with low water availability MP induced the opposite response. This shows that both the degree and direction of plant responses to MP depend on the presence of other factors. We discuss how the interactive effects of MP and water availability on growth and mechanical properties may help C. mongolicum to establish in windy and arid environments.

Effects of Shaking on the Growth and Mechanical Properties ofHedysarum laeveMay Be Independent of Water Regimes

Little is known about how the effects of mechanical perturbation (MP) on clonal semishrubs vary with soil water in inland dune ecosystems. To investigate this, a greenhouse experiment was conducted in which the seedlings of the clonal semishrub Hedysarum laeve were subjected to two levels of MP (non‐MP vs. MP 60 s d−1) and two levels of water supply (200 vs. 400 mL d−1). MP induced plants to yield shorter stems with less rigidity, fewer leaves, thinner basal diameters, and less biomass; increased soil water allowed plants to produce taller stems, more leaves, thicker basal diameters, and more biomass. However, significant MP by water interactions on the growth and mechanical properties of H. laeve were not detected. These findings suggest that the effects of MP on the growth and mechanical properties of semishrubs may be independent of soil water.

An analysis of the effects of stretch on IGF-I secretion from rat ventricular fibroblasts

Mechanical force can induce a number of fundamental short- and long-term responses in myocardium. These include alterations in ECM, activation of cell-signaling pathways, altered gene regulation, changes in cell proliferation and growth, and secretion of a number of peptides and growth factors. It is now known that a number of these autocrine/paracrine factors are secreted from both cardiomyocytes and ventricular cardiac fibroblasts (CFb) in response to stretch. One such substance is IGF-I. IGF-I is an important autocrine/paracrine factor that can regulate physiological or pathophysiological responses, such as hypertrophy. In this study, we addressed the possible effects of mechanical perturbation, biaxial strain, on IGF-I secretion from adult rat CFb. CFb were subjected to either static stretch (3-10%) or cyclic stretch (10%; 0.1-1 Hz) over a 24-h period. IGF-1 secretion from CFb in response to selected stretch paradigms was examined using ELISA to measure IGF-I concentrations in conditioned media. Static stretch did not result in any measurable modulation of IGF-I secretion from CFb. However, cyclic stretch significantly increased IGF-I secretion from CFb in a frequency- and time-dependent manner compared with nonstretched controls. This stretch-induced increase in secretion was relatively insensitive to changes in extracellular [Ca(2+)] or to block of L-type Ca(2+) channels. In contrast, thapsigargin, an inhibitor of sarco(endo)plasmic reticulum Ca(2+) ATPase, remarkably decreased stretch-induced IGF-I secretion from CFb. We further show that IGF-I can upregulate mRNA expression of atrial natriuretic peptide in myocytes. In summary, cyclic stretch can significantly increase IGF-I secretion from CFb, and this effect is dependent on a thapsigargin-sensitive pool of intracellular [Ca(2+)].

Regulation of capillary hydraulic conductivity in response to an acute change in shear

The effects of mechanical perturbations (shear stress, pressure) on microvascular permeability primarily have been examined in micropipette-cannulated vessels or in endothelial monolayers in vitro. The objective of this study is to determine whether acute changes in blood flow shear stress might influence measurements of hydraulic conductivity (L(p)) in autoperfused microvessels in vivo. Rat mesenteric microvessels were observed via intravital microscopy. Occlusion of a third-order arteriole with a micropipette was used to divert and increase flow through a nonoccluded capillary or fourth-order arteriolar branch. Transvascular fluid filtration rate in the branching vessel was measured with a Landis technique. Flow (shear)-induced increases in L(p) disappeared within 20-30 s of the removal of the shear and could be eliminated with nitric oxide synthase inhibition. The shear-induced increase in L(p) was greater in capillaries compared with terminal arterioles. An acute change in shear may regulate L(p) by a nitric oxide-dependent mechanism that displays heterogeneity within a microvascular network.

Oribatid mite and collembolan diversity, density and community structure in a moder beech forest ( Fagus sylvatica): effects of mechanical perturbations

The effects of mechanical perturbations on two soil microarthropod communities (oribatid mites and collembolans) were investigated in a moder beech forest on sandstone. We disturbed the soil matrix by sieving and mixing the litter and soil of the moder profile. The top litter layer (L material) and the deep mineral soil (Bv) remained intact. Three amounts of disturbance were established: a single perturbation, perturbations once every 2 months (60 d) and once every 2 weeks (14 d). Densities of most groups of oribatid mites and all groups of collembolans declined in the disturbance treatments. In most cases, densities were lowest in the strong perturbation treatment (14 d). Desmonomata were the only group of oribatid mites that benefited from intermediate amounts of disturbance but not from the strongest disturbance. Also, disturbances reduced diversity of oribatid mites and collembolans. According to their sensitivity to disturbances oribatid mites ranked Poronota=Enarthronota=Suctobelbidae (the most sensitive)>Oppiidae> Tectocepheus>Desmonomata. The ranking of collembolans was Folsomia (the most sensitive)>Hypogastruridae/Neanuridae>Onychiuridae=Isotomidae>Entomobryidae. Generally, tolerance of disturbance was wider for oribatid mites than for collembolans. The results indicate that disturbances such as mixing of litter and soil and comminution of litter material strongly affect the density and diversity of soil microarthropods. However, they also indicate that the soil microarthropod community is resistant to weaker disturbances. In the field, mechanical disturbances are often caused by burrowing of earthworms. Our results suggest that the high density of microarthropods in moder soils may be due to the low intensities of mechanical disturbances by earthworms.

Effect of mechanical perturbation on the release of PGE(2) by macrophages in vitro.

Macrophages play numerous roles in both physiologic and pathologic processes. Along with fibroblasts, they comprise the synovial tissue that forms the lining of musculoskeletal joint capsules and bursae, and they often envelop implants. During the process of phagocytosing prosthesis-related particles, macrophages in peri-implant tissue release inflammatory mediators. Little is known, however, about the response of these cells to mechanical perturbation, which often is a component of the physical environment of the cell. Mouse peritoneal macrophages were grown on a flexible membrane in vitro and a dynamic 1-Hz spatially uniform sinusoidal strain pattern imparted to the elastomeric substrate. The effect of mechanical strain on prostaglandin (PG) E(2) release was evaluated using cells that were activated by lipopolysaccharide (LPS) as well as by those that were not. The results are compared with the levels of PGE(2) stimulated by metallic particles. Strain magnitudes of 4 and 8% applied for 1 h resulted in almost a twofold increase in the release of PGE(2) from LPS-stimulated cells (p < 0.05) and nonstimulated macrophages (p < 0.07), compared with nonperturbated controls. No release was elicited by a challenge of metal particles. These findings demonstrate for the first time an effect of mechanical force on the release of an inflammatory mediator by macrophages. This response may help to explain the macrophage-mediated processes underlying the osteolysis associated with loose prostheses in bone and suggests a mechanism for the inflammation of synovial tissues by excessive mechanical strain.

Costs to Flowering of the Production of a Mechanically Hardened Phenotype inBrassica napusL.

To examine potential costs of mechanical hardening in plants, dose‐dependent effects of mechanical perturbation on the growth and development of Brassica napus were investigated in a greenhouse experiment. Mechanical perturbation (stem and leaf flexure) was imposed by exposing seedlings to 0, 10, 20, 30, or 40 flexures with a bamboo rod once per day over a period of 35 d. In general, mechanical perturbation reduced plant height and root mass but increased stem diameter while decreasing pith diameter in a nearly linear dose‐dependent manner. In addition, mechanical perturbation delayed both cotyledon senescence and date of anthesis and also reduced flower number in a similar linear dose‐dependent manner. In contrast, effects of mechanical perturbation on final leaf area, leaf mass, and stem mass were not linearly related to dose, although means of these variables were smaller in plants subjected to 40 flexures per day than in all other treatments. Dose‐dependent effects of mechanical perturbation on B. nap...

On the plasticity of the cerebellar renin-angiotensin system: localization of components and effects of mechanical perturbation

This study focuses on the renin-angiotensin system (RAS) in the cerebellar cortex and changes within this system after mechanically induced cerebellar injury. Using radioactive and non-radioactive in situ hybridization and immunocytochemistry angiotensinogen mRNA, angiotensinogen, angiotensin II and, for the first time, N-terminal angiotensin fragment (1–7) immunoreactivities, respectively, were demonstrated in the rat cerebellum. Angiotensinogen mRNA and angiotensinogen immunoreactivity (IR) were both present in glial cell populations of all layers, especially in the Purkinje and granular cell layers and within the cerebellar nuclei. Angiotensin II IR was demonstrated in glial cell population in all layers using a monoclonal angiotensin II antibody, while with a polyclonal angiotensin II antiserum (Denise) some Purkinje cell bodies were labelled. After lesioning the cerebellar cortex mechanically by an injection cannula a strong increase in angiotensinogen gene expression as well as in angiotensin II and angiotensin (1–7) immunoreactivities were observed in the glial cell populations. Furthermore, putative Bergmann glial processes, as indicated from the morphological appearance became strongly angiotensin II and angiotensinogen immunoreactive in the region close to the mechanically induced lesion. It could inter alia be demonstrated for the first time using confocal laser microscopy of ANG II IR and GFAP IR that ANG II in vivo in the intact cerebellar cortex is present in astroglial processes in the molecular layer and presumably secreted into the extracellular space in form of small spheric bodies and/or taken up by other cell types. In contrast, the N-terminal fragment angiotensin (1–7) IR was restricted to the glial cell populations and appeared only after the lesion event. Thus, it is suggested that the cerebellar RAS shows marked changes in response to mechanically induced lesions. The expression of angiotensinogen as well as the production of angiotensinogen IR and angiotensin II like IR is even after mechanical lesion restricted to astrocytes, i.e., cerebellar astrocytes and putataive Bergmann glial cells, and in case of immunoreactivities it spreads to the radially oriented Bergmann glial processes in the molecular layer.

Thigmomorphogenesis: the effect of mechanical perturbation on plants.

Thigmomorphogenetic responses occur in many environmental settings. The most pronounced effects are found under conditions of extremely high rates of turbulent wind or water flow. However, it is an ubiquitous phenomenon, since mechanical perturbations are to be encountered under all but the most stringent laboratory conditions. Our present understanding of these phenomena is the result of studies at the ecological, anatomical, physiological, biochemical, biophysical and molecular biological levels.