Effect Of Long-term Storage Research Articles

Effects of Storage Voltage upon Sodium-Ion Batteries

Sodium-ion batteries (SIBs) are gaining attention as a safer, more cost-effective alternative to lithium-ion batteries (LIBs) due to their use of abundant and non-critical materials. A notable feature of SIBs is their ability to utilize aluminum current collectors, which are resistant to oxidation, allowing for safer storage at 0 V. However, the long-term impacts of such storage on their electrochemical performance remain poorly understood. This study systematically investigates how storage conditions at various states of charge (SOCs) affect open circuit voltage (OCV) decay, internal resistance, and post-storage cycling stability in two different Na-ion chemistries: Prussian white//hard carbon and layered oxide//hard carbon. Electrochemical Impedance Spectroscopy before and after storage shows a pronounced increase in internal resistance and a corresponding decline in cycling performance when SIBs are stored in a fully discharged state (0 V), particularly for layered oxide-based cells, illustrating the sensitivity of different SIB chemistries to storage conditions. Additionally, a novel reformation protocol is proposed that reactivates cell capacity by rebuilding the solid electrolyte interphase (SEI) layer, offering a recovery path after prolonged storage. These insights into the long-term storage effects on SIBs provide new guidelines for optimizing storage and transport conditions to minimize performance degradation, making them more viable for commercial applications.

Effect of long-term storage of cattle manure on its energy potential and biodegradability

Abstract In recent years in Russia, due to the complicated geopolitical situation in some border areas, biogas plants have been considered not only as waste utilization facilities, but also as reserve energy sources, which are safer than traditional - nuclear power plants. In this context, the development of an algorithm for the flexible operation of a biogas plant is particularly relevant, which gives rise to the need to study the stability of the system under the influence of different unfavourable factors. In this work, the influence of long-term storage of cattle manure on its energy potential and biodegradability is studied. The specific methane yield in the test variant with manure stored for 10 months before anaerobic fermentation was 1.41±0.55 ml/g oDM, which is 6.23 times lower than in the test variant with fresh manure; the methane content of manure after long-term storage is 9.66 times lower and the degree of decomposition of its organic matter is 2.72 times lower compared to similar indicators of manure processed without preliminary storage. However, the specific biogas yield from long-term stored manure is 1.66 times higher than the control, which indicates intensive formation of other gases. Thus, long-term storage has a negative impact on the energy potential of cattle manure; if it is necessary to process it in a biogas plant, it is advisable to combine it with more energy-intensive raw materials.

Effects of storage conditions on the stability of qPCR reagents: implications for environmental DNA detection

ObjectiveEnvironmental DNA (eDNA) detection is a transformative tool for ecological surveys which in many cases offers greater accuracy and cost-effectiveness for tracking low-density, cryptic species compared to conventional methods. For the use of targeted quantitative PCR (qPCR)-based eDNA detection, protocols typically require freshly prepared reagents for each sample, necessitating systematic evaluation of reagent stability within the functional context of eDNA standard curve preparation and environmental sample evaluation. Herein, we assessed the effects of long-term storage and freeze–thaw cycles on qPCR reagents for eDNA analysis across six assays.ResultsResults demonstrate qPCR plates (containing pre-made PCR mix, primer-probe, and DNA template) remain stable at 4 °C for three days before thermocycling without fidelity loss irrespective of qPCR assay used. Primer-probe mixes remain stable for five months of − 20 °C storage with monthly freeze-thaw cycles also irrespective of qPCR assay used. Synthetic DNA stocks maintain consistency in standard curves and sensitivity for three months under the same conditions. These findings enhance our comprehension of qPCR reagent stability, facilitating streamlined eDNA workflows by minimizing repetitive reagent preparations.

Long-term stability and the physical and chemical factors predictive for antimicrobial activity in Australian honey.

The growing burden of expired medicines contributes to environmental contamination and landfill waste accumulation. Medicinal honey, with its non-toxic nature and potentially long shelf-life, represents a promising and underutilised therapeutic that avoids some of these issues. However, limited knowledge on how its antimicrobial properties change over time combined with a lack of reliable processes in the honey industry for measuring antimicrobial potential, hinder its clinical adoption. Using a diverse selection of 30 Australian honey samples collected between 2005 and 2007, we comprehensively evaluated their antibacterial and antifungal activity and pertinent physical and chemical properties with the aims of assessing the effect of long-term storage on activity, pinpointing factors associated with antimicrobial efficacy, and establishing robust assessment methods. Minimum inhibitory concentration (MIC) assays proved superior to the standard phenol equivalence assay in capturing the full range of antimicrobial activity present in honey. Correlations between activity and a range of physical and chemical properties uncovered significant associations, with hydrogen peroxide, antioxidant content, and water activity emerging as key indicators in non-Leptospermum honey. However, the complex nature and the diverse composition of honey samples precludes the use of high-throughput chemical tests for accurately assessing this activity, and direct assessment using live microorganisms remains the most economical and reliable method. We provide recommendations for different methods of assaying various honey properties, taking into account their accuracy along with technical difficulty and safety considerations. All Leptospermum and fourteen of seventeen non-Leptospermum honey samples retained at least some antimicrobial properties after 15-17 years of storage, suggesting that honey can remain active for extended periods. Overall, the results of this study will help industry meet the growing demand for high-quality, medicinally active honey while ensuring accurate assessment of its antimicrobial potential.

Effects of long-term storage on properties of perovskite solar cells

The Japan Aerospace Exploration Agency (JAXA) is planning a Space photovoltaics Demonstration eXperiment (SDX) to reveal the tolerance to the space environment of the next-generation solar cells. One of the next-generation solar cells mounted on SDX is a perovskite solar cell (PSC), which is cost-effective and has the potential for high specific power and radiation tolerance. It is necessary to clarify the long-term stability to analyze the characteristics of PSCs in orbit because SDX will be stored for months before and after launch. Therefore, we conducted three storage tests (in air, nitrogen, and low pressure) using encapsulated PSCs. Their performance was evaluated by Light I–V, Dark I–V, and EQE measurements. As a result, almost no degradation was observed at EOL (after 400 days) under all storage conditions. We found that the encapsulated PSCs mounted on SDX are highly stable. We also found that the slight degradation of Pmax within 4 % was due to an increase in series resistance.

Bond strength and marginal adaptation of resin composites and correlations with clinical results

ObjectivesDue to innumerable confounding factors and a high number of types and brands of dental restorative materials, the clinical performance of restorative materials are sought predicted by various in vitro tests. However, only few such tests have been found to correlate well with clinical findings. Thus, the present study determined the in vitro dentin bond strength and marginal adaptation of Class II restorations and correlated the results to their clinical outcomes. MethodsDentin bond strength (µTBS and µSBS) and marginal gap formation of Class II restorations (replica technique and SEM) were measured after 24 h and 6 m water storage using eight combinations of adhesive and resin composite. Clinical outcomes (mean survival time, Hazard Ratio, annual failure rate; n = 10.695) were gained from a data set of a retrospective multicenter study of direct restorations. ResultsSignificant differences were found for dentin bond strength and marginal gap formation between the restorative material groups, and negative effects of long-term storage were observed. µTBS correlated significantly with certain clinical outcomes of Class I restorations, while µSBS correlated with certain clinical outcomes of Class II, III, IV and V restorations. Marginal gap formation in enamel and number of paramarginal fractures correlated with certain clinical outcomes of Class II restorations. SignificanceUsing the same restorative materials in vitro as in vivo, gave significant, but weak correlations between in vitro bond strength or marginal adaptation and clinical outcomes, lending support to the use of in vitro tests in early stages of material selection.

Assessment of the effect of long-term serum storage for retrospective serologic diagnosis of canine monocytic ehrlichiosis (Ehrlichia canis)

There is currently sparse information on the possible effect of long-term storage of serum specimens for the retrospective serodiagnosis of canine monocytic ehrlichiosis (CME). The aim of this study was to assess the agreement between the original serologic outcome and the results of a repeat indirect fluorescent antibody (IFA) assay for the detection of IgG antibodies against E. canis. A secondary aim was to compare the diagnostic performance of two commercially available point-of-care (POC) immunochromatographic (IC) assays. Archived serum samples originally tested as positive (n=66) or negative (n=19) for E. canis IgG antibodies and kept frozen at −20°C for a median of 22 years, were retrospectively examined by IFA and by two POC IC assays. Cohen’s Kappa coefficient (0.748, p < 0.0001), indicated a substantial agreement between the original and repeat serologic testing results. An almost identical high sensitivity and moderate specificity were established for the two POC IC assays. Canine serum specimens on long-term storage may still be of value for seroepidemiologic surveys investigating the exposure to E. canis.

Comparison of bioactive substances in novel-developed freeze-dried platelet-rich plasma (PRP) and activated normal PRP, and investigation of bioactive substance levels after long-term storage

IntroductionIn recent years, biotherapy in orthopedics has become widespread, and platelet-rich plasma (PRP) has been readily used to treat sports injuries and osteoarthritis. Production of freeze-dried PRP (PRP-FD) results in PRP that is in powder form, allowing it to be stored for long periods at room temperature. Using this technology, we have developed Valuable Platelet-Derived Factor Concentrate Freeze Dry (VFD). However, whether VFD contains sufficient levels of bioactive substances (BS) remains unknown and retains the same levels of BS during long-term storage. In this study, we examined whether VFD contains sufficient amounts of BS and whether they retain these BS levels during long-term storage. MethodsPeripheral blood was collected from 10 healthy men (mean ± SD: 46.5 ± 15 years old) and various BS, including transforming growth factor β (TGF-β), basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), tissue inhibitors of metalloproteinases-1 (TIMP-1), interleukin-1 receptor antagonist (IL-1ra), matrix metallopeptidase-9 (MMP-9), and interleukin-6 (IL-6), were compared between VFD and normal PRP samples, including both leukocyte-rich PRP (LR-PRP) and leukocyte-poor PRP (LP-PRP). VFD was prepared using two rounds of centrifugation. LP-PRP and LR-PRP were activated by freezing and thawing before measurement. To evaluate the effects of long-term storage, the BS of VFD purified from five professional football players was compared between baseline and 1 year after storage. ResultsIn terms of the growth factors, the TGF-β and EGF levels were higher in LR-PRP than in VFD and LP-PRP (p < 0.05), while the bFGF levels were higher in VFD than in the LR-PRP and LP-PRP groups (p < 0.01). In terms of anti-inflammatory cytokines, the TIMP-1 level was lower in VFD than that in the other groups (p < 0.01), whereas the IL-1ra levels were higher in VFD than those in LP-PRP (p < 0.05) and lower than those in LR-PRP (p < 0.01). In terms of inflammatory enzymes and cytokines, the IL-1ra level was higher in VFD than that in LP-PRP (p < 0.05) and lower than that in LR-PRP (p < 0.01), whereas the IL-6 levels did not differ among the groups. Furthermore, the TGF-β, bFGF, TIMP-1, and IL-1ra levels were 5.61 → 3.38 (x103 pg/μL), 61.0 → 63.0 (pg/μL), 3.4 → 2.7 (x105 pg/μL), and 14.9 → 14.5 (x103 pg/μL) at baseline and 1 year later, respectively. No significant differences in the BS levels were observed between baseline and 1 year after storage. ConclusionsThe VFD samples prepared in this study exhibited higher levels of anti-inflammatory cytokines than LP-PRP and contained growth factor levels similar to LP-PRP and LR-PRP. In addition, the BS levels in VFD samples were maintained after one year of storage. These results suggest that VFD can be prepared and stored and may serve as a novel treatment strategy for sports injuries in high-risk groups, such as athletes.

The Influence of Long-Term Storage on the Epiphytic Microbiome of Postharvest Apples and on Penicillium expansum Occurrence and Patulin Accumulation.

Patulin is a secondary metabolite primarily synthesized by the fungus Penicillium expansum, which is responsible for blue mold disease on apples. The latter are highly susceptible to fungal infection in the postharvest stages. Apples destined to produce compotes are processed throughout the year, which implies that long periods of storage are required under controlled atmospheres. P. expansum is capable of infecting apples throughout the whole process, and patulin can be detected in the end-product. In the present study, 455 apples (organically and conventionally grown), destined to produce compotes, of the variety "Golden Delicious" were sampled at multiple postharvest steps. The apple samples were analyzed for their patulin content and P. expansum was quantified using real-time PCR. The patulin results showed no significant differences between the two cultivation techniques; however, two critical control points were identified: the long-term storage and the deck storage of apples at ambient temperature before transport. Additionally, alterations in the epiphytic microbiota of both fungi and bacteria throughout various steps were investigated through the application of a metabarcoding approach. The alpha and beta diversity analysis highlighted the effect of long-term storage, causing an increase in the bacterial and fungal diversity on apples, and showed significant differences in the microbial communities during the different postharvest steps. The different network analyses demonstrated intra-species relationships. Multiple pairs of fungal and bacterial competitive relationships were observed. Positive interactions were also observed between P. expansum and multiple fungal and bacterial species. These network analyses provide a basis for further fungal and bacterial interaction analyses for fruit disease biocontrol.

Analysis of changes in moisture levels in broccoli during long-term storage

This paper examines the effect of long-term storage on the moisture content of broccoli. To determine the effect of the long-term storage process on the moisture content of broccoli, this study analyzed the moisture content of several batches of broccoli before and after the long-term storage stage. The measurement was carried out using the method of drying to constant weight at a temperature of 105 °C. The measurement was carried out in three batches of broccoli collected from different agricultural fields. The results obtained allow us to evaluate the level of influence of long-term storage on the change in the % moisture content of broccoli. The results of the study are relevant for further research in the field of improving the quality and environmental friendliness of agricultural products, including through the optimization of long-term storage processes, and can also be used in conducting broader research in the field of agriculture and ecology.

Effects of Long-Term Storage of Pig Slurry on Nitrogen Loss in Different Seasons and Storage Conditions

Highlights The physicochemical properties of the pig slurry in different seasons were different, the contents of COD and total nitrogen of the pig slurry in the summer were significantly higher than the pig slurry in the other seasons. The slurry temperature varied greatly in different seasons, which affected the changes of indicators in pig slurry during long-term storage. Storing pig slurry under sealed conditions was significantly more beneficial in reducing N loss, which was 47%–56% lower than that in the open treatments. Abstract. Pig slurry (PS) can be applied to farmland only in certain periods during the year, so long-term storage is required before field application. The effects of the long-term storage of the PS on N loss in different seasons and under different storage conditions (open and sealed storage) were investigated in this study. The physicochemical properties and slurry temperature varied greatly in different seasons. The electrical conductivity, chemical oxygen demand, ammonium nitrogen, nitrate nitrogen, total nitrogen (TN), and heavy metals (Cu, Zn, Pb, As, and Cd) decreased after 150 days of storage, while the pH value increased, and all these indicators (except for the heavy metals) were affected by the slurry temperature, exhibiting a larger decrease at higher slurry temperatures. The decrease in the N loss was 47%–56% lower in the sealed treatments than in the open treatments. The As, Pb, and Cd contents of the stored PS (except for the AU-OP treatment) met the requirements specified for GB/T 40750-2021. Overall, storing PS under sealed and lower temperature conditions was conducive to reducing N loss and improving the N content of the stored PS. Keywords: Heavy metal, Nitrogen loss, Open storage, Pig slurry, Sealed storage, Temperature.

Fluid preservation in brain banking: a review.

Fluid preservation is nearly universally used in brain banking to store fixed tissue specimens for future research applications. However, the effects of long-term immersion on neural circuitry and biomolecules are not well characterized. As a result, there is a need to synthesize studies investigating fluid preservation of brain tissue. We searched PubMed and other databases to identify studies measuring the effects of fluid preservation in nervous system tissue. We categorized studies based on the fluid preservative used: formaldehyde solutions, buffer solutions, alcohol solutions, storage after tissue clearing, and cryoprotectant solutions. We identified 91 studies containing 197 independent observations of the effects of long-term storage on cellular morphology. Most studies did not report any significant alterations due to long-term storage. When present, the most frequent alteration was decreased antigenicity, commonly attributed to progressive crosslinking by aldehydes that renders biomolecules increasingly inaccessible over time. To build a mechanistic understanding, we discuss biochemical aspects of long-term fluid preservation. A subset of lipids appears to be chemical altered or extracted over time due to incomplete retention in the crosslinked gel. Alternative storage fluids mitigate the problem of antigen masking but have not been extensively characterized and may have other downsides. We also compare fluid preservation to cryopreservation, paraffin embedding, and resin embedding. Overall, existing evidence suggests that fluid preservation provides maintenance of neural architecture for decades, including precise structural details. However, to avoid the well-established problem of overfixation caused by storage in high concentration formaldehyde solutions, fluid preservation procedures can use an initial fixation step followed by an alternative long-term storage fluid. Further research is warranted on optimizing protocols and characterizing the generalizability of the storage artifacts that have been identified.

How to store a beetle larva? Comparing temporal effects of common fluid preservation methods on color, shape, and DNA quality.

Proper fixing and long-term preservation of entomological evidence are essential in collections and research and crucial in applied fields such as forensic entomology. Incorrectly stored samples may lose important morphological features over time, rendering molecular analyses exceedingly difficult. The most effective method for preserving soft samples such as larvae is fluid preservation. It uses a combination of a wide range of fixatives and storage fluids. However, very little comparative work has been done to determine the effects of long-term storage on sample quality in terms of color, shape, and DNA stability. Moreover, the current golden standard in forensic entomology has been tailored for age estimation of larvae of Diptera, which differ from larvae of Coleoptera in morphology and subsequently in applied methods. We compared the effects of combinations of 6 commonly used fixatives and 6 commonly used storage fluids on midsized larvae of the forensically important beetle, Necrodes littoralis (Linnaeus, 1758), in terms of color, shape, and suitability for DNA analyses over a 2-yr period. We were looking for combinations that can preserve specimens in a satisfactory state, can be used on a regular basis, do not require advanced protection or skills of the personnel, and are not toxic or too harmful to the environment. We found not only several methods that scored significantly better in the tested parameters compared with the golden standard but also several common methods that should be avoided. The effects of agents on each tested category are discussed in detail.

Diterpenes stability of commercial blends of roasted and ground coffees packed in copolymer coupled with aluminium and eco-friendly capsules

Diterpenes are group of compounds of the terpenic fraction of roasted coffee and account for about 7–20 % (w/w) of the lipid fraction. Several parameters can influence their occurrence in coffee beans and beverages including species and post-harvest processing. Diterpenes in coffee have been studied extensively, but to the best of the authors’ knowledge, there is no information in the literature on their stability over time. Coffee is a relatively stable product under optimal temperature, humidity and oxygen conditions. However, during storage it can undergo a series of chemical and physical reactions that alter its flavour and lead to rancidity, mainly due to the oxidative reactions that take place on the lipid fraction. In this study, the effect of long-term storage on the diterpene content of different commercial coffee blends and packaging is analysed and critically discussed. The Results show that the storage influences the internal environment of the capsules with an increase in moisture and a decrease in pH favouring more reactive conditions, especially for Eco capsules. Relative stability over time is observed for cafestol and kahweol. dehydro derivatives show a degradation up to T60 independently on the blends and packaging, which is not related to their precursors. The permeability of packaging and blends affect the modification of these components: while a drastic oxidation process takes place in Arabica eco compatible capsules (PC) when acidity and moisture increase, in Arabica/Robusta eco compatible capsules (IC) as well as in Arabica/Robusta and Arabica standard capsules (IS and PS) the peroxides tend to increase resulting in an autocatalytic propagation.

Folding, stowage, and deployment of composite thin-walled lenticular tubes

Composite thin-walled lenticular tube (CTLT) is a lightweight foldable and deployable structural material that enables large-scale deployable mechanisms for various space missions. A key step toward the structural design of CTLT is to understand its folding, stowage, and deployment behaviors. This work presents an integrated experimental and numerical investigation of the dynamic deployment behavior of CTLT that wraps around a central hub, with emphasis on the effect of long-term storage. A two-meter-long CTLT prototype was manufactured, and a gravity compensation system was designed and built for the on-ground dynamic deployment experiments. The deployment experiments were performed on the CTLT prototype both before and after it had been stowed for extended storage periods. The results indicate that after being stowed for 6 and 10.5 months the CTLT is deployed slower and the deployment time increases by 8.2% and 15.0%, respectively. Furthermore, a high-fidelity numerical model was constructed using the explicit dynamic finite element method, where the CTLT was modeled as a deformable part and the folding/deployment mechanisms were modeled as rigid bodies to perform the folding, stowage, and deployment simulations. The long-term storage effect was accounted for in the numerical analyses with the use of a viscoelastic fiber-reinforced composite material model, and a good agreement has been achieved between the experimental and numerical results.

Detection of adeno-associated viral DNA in equine post-administration frozen blood and plasma samples after long-term storage.

Gene doping in horses is a threat to the fairness in sport and has serious implications for animal welfare. To investigate the effect of long-term storage on the detection of AAV in plasma and whole blood, samples from an administration study using an adeno-associated virus serotype 6 expressing green fluorescence protein (AAV6-GFP) were stored at -20°C for 8months before analysis. The AAV vector was detected in stored plasma samples, following the same detection profile as the fresh plasma samples. The stored blood showed lower overall DNA detection but followed the same detection profile as the plasma samples. This study provides confidence that re-analysing plasma samples and/or analysing a frozen 'B' sample with different matrix such as whole blood after prolonged storage will still result in the detection of gene doping material.

Effect of long-term storage on glucosinolate and S-methyl-l-cysteine sulfoxide hydrolysis in cabbage (Brassica oleracea var. capitata)

Cabbages are good sources for glucosinolates and S-methyl-l-cysteine sulfoxide (SMCSO), precursors to bioactive volatile hydrolysis products such as isothiocyanates, sulfides and thiosulfinates. Often, white and red cabbages are stored at 0 °C for many months before being sold. Here, we investigated the effect of storage for up to eight months on glucosinolates, SMCSO and the formation of isothiocyanates and derived amines, (epithio)nitriles and volatile organosulfur compounds (VOSCs) in white and red cabbages. Further, the effect of storage on protein expression was evaluated. Overall, glucosinolates and well as SMCSO contents were stable during storage. While in white cabbage glucosinolate hydrolysis was not much affected, in red cabbage storage increased formation of isothiocyanates and methylthioalkylamines, which was linked with reduced epithiospecifier protein 1 abundance. Long-term storage reduced formation of VOSCs from SMCSO which correlated with decline in predicted cystine lyase. Therefore, storage maintains these phytonutrients and can increase formation of health-promoting isothiocyanates.

A Solution for Ambient Storage of DNA

The need for a solution for room temperature storage of DNA is well known. Studies were conducted to assess stability of EBV DNA using Daykin Molecular Solutions GeneGuardDNA (Georgia). All laboratory testing was done at Prime Laboratories in Van Nuys California. The objective is to demonstrate sample stability of EBV DNA during shipping, storage, and multiple freeze/thaw cycles. To achieve the objectives, the study was divided into two parts; Part 1 to demonstrate stability during shipping and multiple freeze/thaw cycles. Samples were labeled as P1-1 (Positive sample 1, P1-2 first freeze/thaw, P1-3 second freeze/thaw, etc.). In Part 1 the standard deviation between positive samples ranged from 0.08 CTs to 0.25 CTs with no gradual deterioration detected with more freeze/thaw cycles). The average was 0.17 CT’s which is within the instrument’s precision range. In the past we have had no instance of repeating the same extracted sample more than two times. In Part 2 we demonstrated stability during storage. 185 archived clinical samples stored at room temperature from 2013 through 2017 were randomly chosen. DNA was extracted from these samples and EBV DNA assay testing was performed. The results from the new test were compared to the original results obtained for the samples. Concordance and p values were calculated to show the effects of long-term storage on the results of the EBV DNA assay. Pearson correlation coefficient between the original and recent assay results was 0.9564 with a p value of p&lt;0.001 indicating statistically significant correlation between the two assay results. EBV DNA showed no deterioration of sample Ct signal was observed due to Freeze/Thaw cycles and the results were stable across multiple cycles for a period of 6 years at room temperature.

Effects of Long-Term Storage on Hatchability and Incubation Length of Game Farmed Quail Eggs.

The long-term storage of eggs before incubation is a common practice in some alternative poultry systems but needs to be performed under conditions that preserve egg viability. The effects of the long-term storage of game farmed quail (Coturnix coturnix) eggs on weight loss during the storage and incubation periods, chick weight at hatch, hatchability, and incubation length were investigated. The eggs were arranged in six treatments submitted to 0-, 7-, 14-, 21-, 28-, and 35-day storage periods at 15.8 °C and 80% relative humidity. The storage length reduced the hatchability of eggs (p < 0.05) when the storage was extended to 35 days, decreasing by more than half compared to eggs stored up to 28 days. Egg weight loss during storage progressively increased with the storage length (p < 0.05). Chick weight at hatching was reduced in eggs stored for more than 14 days (p < 0.05), and relative chick weight decreased significantly in eggs stored for 35 days (p < 0.05). Incubation length progressively increased with the storage length (p < 0.05), achieving less hatching synchrony in eggs stored for a longer time (p < 0.05). In conclusion, game quail eggs store well with little deterioration up to 28 days at 15.8 °C and 80% relative humidity, allowing for extended storage when shipping long-shelf-life eggs or assembling batches large enough to fully set an incubator in farms with small breeding flocks.

Response of the Endometrial Mesenchymal Stem Cell Genome to the Procedure of Long-Term Cryopreservation

Information about the effect of cryopreservation on functions and the genetic of cells of different genesis is not unambiguous and is in the process of accumulation. This work is aimed at studying the effect of long-term storage (7 years) of human endometrial mesenchymal stem cells (eMSCs) in the frozen state on the stability of their genome in vitro. The results showed destabilization of the karyotype structure in the descendants of cells after their thawing, namely, aneupolyploidization, increased fragility of chromosomes, resulting in a huge pool of aberrant chromosomes, and impaired condensation in homologues. Chromosomal breakds in centromeric regions offen accompanied by the preservation of genetic material in the form of independent chromosomes. Almost all chromosomes of the set were involved in the process of destabilization of the eMSCs cell genome. It has been shown that the procedure of long-term cryopreservation can become an inducer of premature cellular aging of eMSCs after their thawing. Comparison of the data obtained with the results of karyotyping of transformed Chinese hamster cells that underwent a similar procedure led to the conclusion that cryopreservation for biological systems can be a stress that induces genetic defects of various types at the karyotype level. The response of the genome of cells of different origin to the same conditions of cryopreservation may differ.