Effects Of Long-term Manure Application Research Articles

Long-term manure and cropping systems effect on soil water vapour sorption characteristics is controlled by soil texture

Increasing studies indicate that long-term experiments (LTEs) involving manure application and optimal cropping systems can significantly improve soil physicochemical properties, which may also affect the soil water retention curve (WRC) in the wet region (pF < 4.2). However, few studies have explored the effect of long-term manure application and cropping systems on soil WRC in the dry region (water vapour sorption isotherms, WSIs) (pF > 4.2) as well as the associated soil physicochemical properties. To overcome this knowledge gap, we investigated the effect of long-term manure application (24 to 177 years) and different cropping systems (71 to 82 years) on soil organic carbon (SOC) content, WSIs, hysteresis, and specific surface area (SSA). Soil samples were collected from five long-term manure experiments in Sweden, the United Kingdom, Spain, Germany, and Denmark, and two long-term cropping systems experiments in the United Kingdom. The five manure LTEs comprised three soil textures (silty clay, silt loam, and sandy loam) with one to four crops grown in the fields, including wheat, barley, maize, and grass/clover. The cropping system LTEs comprised silt loam and sandy loam including bare fallow (BF), arable rotation, ley–arable rotation, and permanent grass. Results showed that long-term manure application increased SOC content in each site, but it had little effect on the soil WSIs, hysteresis, and SSA in silty clay soils. The changes of soil WSIs, hysteresis, and SSA in silt loam and sandy loam arising from manure application largely depend on the crops grown in the field. For the long-term cropping systems experiments, permanent grass had the most significant effect in increasing SOC content, soil WSIs in silt loam and sandy loam, and hysteresis and SSA in silt loam compared to other treatments. Compared to BF, arable rotation and ley-arable rotation had no effect on SOC and soil WSIs in silt loam, and on hysteresis and SSA in silt loam and sandy loam. Multiple linear regression models including SOC, clay, and silt contents sufficiently explained the variabilities observed in the soil WSIs, hysteresis, and SSA for both manure and cropping systems LTEs.

Legacy phosphorus in Alabama Hartsells soil after long-term amendment with broiler litter.

Numerous studies have investigated effects of long-term manure application on total phosphorus (P) and inorganic P (Pi ), but few have evaluated soil organic P (Po ). Little is known about crop management effects on Po in soils with varying minerology. In this study, sequential fractionation was used to characterize specific P forms after 25 years of broiler litter (BL) or ammonium nitrate (Con) applications to an Alabama Hartsells soil. Crops (corn [Zea mays L.], soybean [GlycineWilld.], and corn or soybean with a wheat [Triticum aestivum L.] cover crop) were under conventional tillage (CT) or no-tillage (NT). Regardless of crop, tillage, or fertilizer type, the proportion of extractable Pi was relatively stable at 21%-49% at 0-5cm and 25%-45% at 5-10cm. Extractable Pi ranged from 0.69 to 2.4mg g-1 . BL increased total extractable Pi (p≤0.001) at 0-5cm and 5-10cm. Total extractable P was influenced at 0-5cm (p≤0.006) by both tillage and fertilization type, but not at 5-10cm or at either depth in soybean plots. Long-term BL application increased total extractable soil P at 0-5cm. In corn systems, CT did not reduce P loading to topsoil or result in P leaching to lower soil depths, compared to NT. Soybean and soybean-wheat reduced P loading in BL plots, compared to corn and corn-wheat. Soil Po was classed in the order of monoesters >phytate and polyphosphates, where most was extractable with NaOH. BL increased extractable Po in all fractions. Care should be taken when applying BL to highly weathered soils to avoid legacy Po accumulation. Soybean rotations and cover crops could help remediate P-laden soils after repeated BL application.

Long-Term Manure Application Changes Bacterial Communities in Rice Rhizosphere and Arsenic Speciation in Rice Grains.

Bioavailability and speciation of arsenic (As) are impacted by fertilization and bacteria in the rice rhizosphere. In this study, we investigated the effects of long-term manure application on As bioavailability, microbial community structure, and functional genes in a rice paddy field. The results showed that manure application did not affect total As in the soil but increased soluble As forms by 19%, increasing arsenite (As(III)) accumulation in rice grains and roots by 34 and 64% compared to a control. A real-time quantitative polymerase chain reaction (qPCR) and high-throughput sequencing analysis demonstrated that manure application increased the relative abundance of Rhizobium, Burkholderia, Sphingobium, and Sphingomonas containing arsenate reductase genes (arsC) in the rhizosphere soil, consistent with the 529% increase in arsC, which may have promoted arsenate (As(V)) reduction and increased As availability in pore water. In addition, manure application significantly altered the iron (Fe)-plaque microbial community structure and diversity. The microbes, particularly, Bradyrhizobium, Burkholderia, and Ralstonia, were mostly associated with As, Fe, and sulfur (S) cycles. This result was consistent with changes in the functional genes related to As, Fe, and S transformation. Although manure application promoted As(V) reduction (arsC) in Fe-plaque by 682%, it inhibited Fe and S reduction by decreasing FeIII reduction bacteria (Geobacteraceae) and the sulfate-reducing gene (dsrA) abundance. Further, manure application changed the composition of the microbial community that contained the arsC gene. In short, caution needs to be excised even in the soil with a low As concentration as manure application increased As(III) accumulation in rice grains.

Assessing Pathogen Presence in an Intensively Tile Drained, Agricultural Watershed.

Increases in swine production and concomitant manure application provide beneficial nutrients for crops but also include the potential to spread pathogenic bacteria in the environment. While manure is known to contain a variety of pathogens, little is known regarding the long-term effect of manure application on fate and transport of this diverse set of pathogens into surrounding waterways. We report on the use of 16S-rRNA gene sequencing to detect pathogen-containing genera in the agriculturally dominated South Fork Iowa River watershed, home to approximately 840,000 swine in the 76,000-ha basin. DNA was extracted from monthly grab samples collected from three surface water sites and two main artificial drainage outlets. DNA sequences from water samples were matched with sequences from genera known to contain pathogens using targeted 16S rRNA amplicon sequencing. The specific genera known to contain pathogens were quantified by combining percentage of genera sequence matches with 16S rRNA gene quantitative polymerase chain reaction results. Specifically, abundances of , , and significantly increased in surface water after typical fall manure application. Additionally, the likely transport pathways for specific genera known to contain pathogens were identified. Surface water concentrations were influenced mainly by artificial drainage, whereas was primarily transported to surface waters by runoff events. The results of this study will help us to understand environmental pathways that may be useful for mitigation of the diverse set of pathogenic genera transported in agroecosystems and the capability of manure application to alter existing microbial community structures.

Increased occurrence of heavy metals, antibiotics and resistance genes in surface soil after long-term application of manure

The purpose of this study was to investigate the impact of long-term application of pig manure on the accumulation of heavy metals, antibiotics and ARGs in surface soil sampled from the Jiaxing long-term field experimental site with three manure treatments, N-PM (0 kg/ha/y, dw), L-PM (7720 kg/ha/y, dw), and H-PM (11,580 kg/ha/y, dw), in 2013 and 2014. The results showed that most serious metal pollution of Zn and Cu was recorded in all manured samples in both years, and their contents exceeded the soil quality standards. Among the three tetracyclines, chlortetracycline was the predominant antibiotic detected with a range of 3.04–98.03 μg·kg−1 in 2013 and 28.67–344.74 μg·kg−1 in 2014 after long-term pig manure application. Q-PCR results showed that the average accumulation of ribosomal protection protein genes (tetM, tetO, tetQ and tetW) was lower than most of the efflux pump genes (tetA and tetG). The abundance of tet and sul genes of those sites with manure application was significantly higher than that of sites without manure application in both years. Metagenomics analysis of ARGs revealed that the abundance of multidrug resistance genes was the most abundant subtype, followed by fluoroquinolone, bacitracin, sulfonamide and tetracycline. There was a positive correlation between the levels of ARGs; soil organic matter, antibiotics, Cu, As, and Zn levels in both years. These results may shed light on the mechanism underlining the effects of long-term manure application on the occurrence and dissemination of ARGs in surface soil.

Ammonia oxidizers and nitrite-oxidizing bacteria respond differently to long-term manure application in four paddy soils of south of China

Nitrification plays an important role in the soil nitrogen (N) cycle, and fertilizer application may influence soil nitrifiers' abundance and composition. However, the effect of long-term manure application in paddy soils on nitrifying populations is poorly understood. We chose four long-term manure experimental fields in the south of China to study how the abundance and community structure of nitrifiers would change in response to long-term manure application using quantitative PCR and Miseq sequencing analyses. Our results showed that manure application significantly increased ammonia oxidizing archaea (AOA) abundance at the ChangSha (CS) and NanChang (NC) sites, while the abundance of ammonia oxidizing bacteria (AOB) represented 4.8- and 12.8- fold increases at the JiaXing (JX) and YingTan (YT) sites, respectively. Miseq sequencing of 16S rRNA genes indicated that manure application altered the community structure of nitrifying populations, especially at the NC and YT sites. The application of manure significantly changed AOA and nitrite oxidizing bacteria (NOB) community structures but not those of AOB, suggesting that AOA and NOB may be more sensitive to manures. Variation partitioning analysis (VPA) and redundancy analysis (RDA) indicated that soil pH, TN, NO3−-N and water content were the main factors in shaping nitrifying communities. These findings suggest that nitrifiers respond diversely to manure application, and soil physiochemical properties play an important role in determining nitrifiers' abundance and communities with long-term manure addition.

Long-term Effect of Manure Application in a Certified Organic Production System on Soil Physical and Chemical Parameters and Vegetable Yields

In 1999, West Virginia University (WVU) established an organic farming systems project with a market garden section consisting of 32 plots measuring 16 × 25 ft arranged in a completely randomized design. Sixteen of these plots were managed as high-input and 16 as low-input plots. High-input plots received 10 tons/acre per year of dairy manure and a rye-vetch (Secale cereale and Vicia villosa) cover crop during each winter season since the inception of the experiment in 1999. Fertility in low-input plots was managed solely with an annual rye-vetch cover crop while both treatments also received 5 tons/acre of mixed species hay used as mulch in 2 of every 4 years. A 4-year rotation of crops, green bean (Phaseolus vulgaris), zucchini (Cucurbita pepo), tomato (Solanum lycopersicum), green pepper (Capsicum annuum), and lettuce (Lactuca sativa) in the Fabaceae, Cucurbitaceae, Solanaceae, and Asteraceae families, was established in 1999 and has been maintained ever since. Soil organic matter (SOM) in the upper 6 inches of the soil profile (4.4% in 1999) has remained unchanged in low-input plots at 5.2% in 2004 and 5.4% in 2014, the year following transition and most recent data collection, respectively. During this same time period, significant increases in SOM from 6.4% in 2004 to 8.7% in 2014 were observed in high-input plots. Bulk density was lower in high-input plots than low-input plots in 2014. Despite these improvements in soil quality, high-input plots showed very high levels of phosphorus and potassium. Over the duration of the experiment, yearly manure application increased yields by 22% in all crops combined; however, individual crops responded quite differently. The yield was 9%, 25%, 24%, and 24% higher in high-input plots than in low-input plots for tomato, pepper, zucchini, and green bean, respectively. Manure application in addition to green manures and hay mulch incorporation was found to result in significant economic returns.

Manure application has an effect on the carbon budget of a managed grassland in southern Hokkaido, Japan

The carbon (C) budget of managed grassland in a cool-temperate region of Japan was estimated using a combination of eddy covariance and the biometric method for five years, to evaluate the effect of manure application. Chemical fertilizer was applied to the fertilizer (F) plot at a rate of 79 ± 20 kg N ha−1 yr−1. In the manure (M) plot, dairy cattle manure was applied at a rate of 10 Mg fresh matter ha−1 yr−1 (1923 ± 407 kg C ha−1 yr−1, 159 ± 68 kg N ha−1 yr−1). There was no significant difference in seasonal gross primary production (GPP) and harvest between the treatment plots, indicating that both fertilizer and manure can increase the biomass production. Annual net ecosystem production (NEP) and ecosystem respiration (RE) was significantly different between the treatment plots. The difference in RE, and between M and F plots approximates heterotrophic respiration of manure (RHm), which ranged from 0.9 to 1.3 Mg C ha−1 yr−1. Average annual RHm was 1.1 ± 0.4 Mg C ha−1 yr−1, and accounted for 56% of the total amount of applied manure C. The annual net biome production (NBP) in the M plot (from 0.0 to 1.5 Mg C ha−1 yr−1) was significantly higher than in the F plot (−1.4 to 0.5 Mg C ha−1 yr−1). The long-term effect of manure application combined with chemical fertilizer did not reduce grass production compared with chemical fertilizer only; however, manure application decreased the NEP throughout manure decomposition, and long-term manure application enhanced the NBP.

Long-term effects of manure application on grain yield under different cropping systems and ecological conditions in China

SUMMARYRice (Oryza sativaL.), wheat (Triticum aestivumL.) and maize (Zea maysL.) are the main crops grown in China. Applying organic manures is an important practice in sustaining soil fertility and agricultural productivity in these cropping systems. The current paper presents the effects of manure application on grain yields in nine long-term experiments that consist of one continuous maize, four wheat–maize and four rice-based cropping systems across a wide range of agro-ecological regions in China. The study shows that regular manure application can increase soil organic carbon (SOC) and grain yield across all the sites. Overall, regular use of manure results in larger increases in SOC in the maize and wheat–maize systems than in the rice-based systems. Application of manure tends to increase the grain yield in the maize and wheat–maize systems during the final years, but increases the grain yield in the rice-based systems during the initial years of the long-term experiments. There is only one site that shows significant improvement in the yield trend in association with the application of manure. The effects of manure on yield trends are probably determined by the initial yield and/or the ‘organic C effect’ that may cause gradual improvements in SOC and soil physical properties.