AbstractDue to the environmental consequences of annual‐dominated cropping systems, there is an increasing need to identify agronomic strategies that incorporate perennial crops. One strategy for increasing perennial cover is through the targeted use of annually harvested perennial food and bioproduct crops in buffer strips, which has the potential to create new revenue streams for farmers and substantially mitigate agricultural nutrient pollution from conventional cropping systems. As buffers are typically installed on marginal land, it is critical to understand how landscape position influences the success of perennial crops. The objectives of this study were to determine the relatively early influence of landscape position on the productivity of a variety of perennial crops and their subsequent soil nutrients and soil water storing capabilities. In this experiment, nine perennial (alfalfa, alsike clover, indiangrass, switchgrass, big bluestem, prairie cordgrass, intermediate wheatgrass, high‐diversity polyculture, low‐diversity polyculture) and two annual (corn, soybean) crops were planted across two landscape positions (hillslope and deposition). Plant biomass, plant tissue nitrogen, soil moisture, and soil NO3‐N and NH4‐N were measured and compared at two different locations in Minnesota. Overall, the polyculture mixes, and to some extent intermediate wheatgrass, performed the best with respect to biomass production while also providing ecosystem services across most soil by landscape position combinations tested in this study. However, there were some important findings specific to each soil and landscape position combination, mainly oriented toward biomass production. We also observed temporal patterns in soil moisture and depth‐related patterns in soil N reductions. This study presents an opportunity to optimize the use of perennial crops on marginal agricultural lands for improved environmental and economic benefit.