Inconsistent Effects Research Articles

Continuous theta burst stimulation over right cerebellum for speech impairment in Parkinson's disease: study protocol for a randomized, sham-controlled, clinical trial.

Speech impairment is a common symptom of Parkinson's disease (PD) that worsens with disease progression and affects communication and quality of life. Current pharmacological and surgical treatments for PD have inconsistent effects on speech impairment. The cerebellum is an essential part of sensorimotor network that regulates speech production and becomes dysfunctional in PD. Continuous theta-burst stimulation (cTBS) is a non-invasive brain stimulation technique that can modulate the cerebellum and its connections with other brain regions. To investigate whether cTBS over the right cerebellum coupled with speech-language therapy (SLT) can improve speech impairment in PD. In this randomized controlled trial (RCT), 40 patients with PD will be recruited and assigned to either an experimental group (EG) or a control group (CG). Both groups will receive 10 sessions of standard SLT. The EG will receive real cTBS over the right cerebellum, while the CG will receive sham stimulation. Blinded assessors will evaluate the treatment outcome at three time points: pre-intervention, post-intervention, and at a 12-week follow-up. The primary outcome measures are voice/speech quality and neurobehavioral parameters of auditory-vocal integration. The secondary outcome measures are cognitive function, quality of life, and functional connectivity determined by resting-state functional magnetic resonance imaging (fMRI). This trial will provide evidence for the efficacy and safety of cerebellar cTBS for the treatment of speech impairment in PD and shed light on the neural mechanism of this intervention. It will also have implications for other speech impairment attributed to cerebellar dysfunctions. www.chictr.org.cn, identifier ChiCTR2100050543.

Testing a Polygenic Risk Score for Morphological Microglial Activation in Alzheimer's Disease and Aging.

Neuroinflammation and the activation of microglial cells are among the earliest events in Alzheimer's disease (AD). However, direct observation of microglia in living people is not currently possible. Here, we indexed the heritable propensity for neuroinflammation with polygenic risk scores (PRS), using results from a recent genome-wide analysis of a validated post-mortem measure of morphological microglial activation. We sought to determine whether a PRS for microglial activation (PRSmic) could augment the predictive performance of existing AD PRSs for late-life cognitive impairment. First, PRSmic were calculated and optimized in a calibration cohort (Alzheimer's Disease Neuroimaging Initiative (ADNI), n = 450), with resampling. Second, predictive performance of optimal PRSmic was assessed in two independent, population-based cohorts (total n = 212,237). Finally, we explored associations of PRSmic with a comprehensive set of imaging and fluid AD biomarkers in ADNI. Our PRSmic showed no significant improvement in predictive power for either AD diagnosis or cognitive performance in either external cohort. Some nominal associations were found in ADNI, but with inconsistent effect directions. While genetic scores capable of indexing risk for neuroinflammatory processes in aging are highly desirable, more well-powered genome-wide studies of microglial activation are required. Further, biobank-scale studies would benefit from phenotyping of proximal neuroinflammatory processes to improve the PRS development phase.

Cutting height of main crop has profound effects on cadmium but not arsenic concentration of ratoon crop in rice

ContextRice contamination with heavy metals seriously threatens human health. Ratoon rice plays a vital role in food security in China, whereas limited information is available on its heavy metal accumulation. Research questionCutting height of main crop is an important crop management practice affecting the growth and yield of ratoon crop (RC), whereas its effects on heavy metals accumulation in RC is still unknown. MethodsField experiments were conducted in 2019 and 2020 to investigate the effects of different cutting heights (high: 45 cm, medium: 15 or 25 cm, and low: 5 cm) on cadmium (Cd) and arsenic (As) concentrations in RC. ResultsCutting height had small and inconsistent effects on As concentration in RC. However, grain Cd concentration of RC in high and medium cutting heights were significantly reduced by 87.5 % and 79.8 % than that in low cutting height, respectively. Similarly, Cd concentrations of stubble and straw at maturity were also significantly lower in high and medium cutting heights than in low cutting height. Regenerated tillers in the low node position were responsible for the differences in Cd concentration among the cutting heights. ConclusionThe increase in cutting height of main crop significantly decreased Cd concentration but has small effects on As concentration in RC. ImplicationsOur results suggest that high cutting height during the harvest of main crop should be considered when rice ratooning is practiced in the environment with Cd-contaminated soil.

Abstract P3119: Identifying The Windows Of Cardiac Risk And Opportunity In Menopause

Post-acute myocardial infarction (AMI) mortality has significant sex differences with a bias towards higher rates in women. While treatment inequities and access to care profoundly impact outcomes, biological factors also contribute to survival discrepancies. One complicating factor that drives worse outcomes in women is menopause. Understanding the biological basis for AMI mortality risk and the exacerbating role of menopause has been hampered by the lack of an animal model that recapitulates the dynamic changes associated with the perimenopausal transition. We used a mouse model of menopause in which ovarian failure is gradually induced following treatment with 4-vinylcyclohexene diepoxide (VCD, 160 mg/kg, 15d), allowing for examination of cardiac alterations established during perimenopause. Echocardiography revealed no contractile changes during perimenopause, but cardiac myofilament activation was significantly affected. Calcium re-uptake by SERCA was impaired by the end of perimenopause. Changes in calcium handling were associated with alterations in calcium handling protein expression and phosphorylation. Activation of RISK and SAFE signaling molecules that protect against ischemia-reperfusion (IR) injury was altered throughout perimenopause, and the RISK-SAFE response to IR differed at points during perimenopause. The myocardial response to cardioprotective estrogen receptor activation also varied throughout perimenopause. Together these data reveal significant, nonlinear alterations in myocardial physiology and endogenous protective signing pathways during perimenopause. Disruptions in endogenous protection and calcium handling are established prior to menopause, providing possible mechanisms for worse post-menopausal AMI outcomes. The altered response to estrogen receptor activation preceding menopause may explain the inconsistent effectiveness of post-menopausal estrogen replacement therapy.

Deterministic processes have limited impacts on foliar fungal endophyte communities along a savanna-forest successional gradient

Patterns and drivers of succession provide insight into the mechanisms that govern community assembly, but remain poorly understood for microbial communities. We assess whether successional trends of trees are mirrored by foliar endophyte communities of three tree species across a deterministic woody successional gradient. Additionally, we test the relative contribution of abiotic predictors, biotic factors, and spatial distance between sites in predicting composition and richness of endophyte communities. Unlike the tree community, endophyte communities showed no consistent evidence of deterministic succession. Host identity was the most important factor structuring endophyte community composition; within hosts, spatial distance from the indigenous forest and between samples was important, while environmental predictors had small and inconsistent effects. Much variation in endophyte composition remained unexplained. In contrast, endophyte richness was well-explained by predictor variables. Host identity was most important in predicting endophyte richness, while the effect of other predictors on richness differed between host species. We conclude that deterministic succession in trees did not result in deterministic succession in endophyte communities; instead community assembly was most strongly influenced by host identity; while within hosts, neutral processes may be more important for endophyte assembly than deterministic factors.

Experimental study of real-time temperature-dependent nonlinear deformation of sandstone

In contrast to thermal heating, the real-time heated rock exhibits significant nonlinearity during the elastic deformation stage. Hence, an experiment assembled by a high and low-temperature test chamber and a microcomputer-controlled rock direct shear instrument is built to evaluate the effect of real-time temperature on the nonlinear deformation behavior of rock. A set of nonlinear stress–strain relationships of forty sandstone samples under moderate real-time temperature conditions are obtained from experiments and agree well with the theoretical predictions from the two-part Hooke’s model (TPHM). The soft and hard parts respond to the real-time temperature are quite different. The ratio of the soft part (γt) increases and the elastic modulus of the hard part (Ee) decreases with the temperature below 125℃. On the contrary, γt decreases and Ee increases with the temperature above 125℃ up to 200℃. Thermally-induced and expandable microcracks (thermal weakening) incur structural damage of the hard part below 125℃, while thermal expansion (thermal strengthening) can lead to tighter compaction between grains above 125℃. Therefore, there is a threshold temperature 125℃ under real-time heating condition due to the thermal cracks and expansion. The elastic modulus of the soft part (Et) decreases continuously with increasing temperature, indicating inconsistent thermal expansion effects on the soft part with mixture of impurity particles and the hard part whose mineral particles are closely contacted at higher temperature. Based on the experimental data and TPHM, empirical relationships between the real-time temperature and nonlinear deformation are proposed. This study will provide support for the safe production and disaster prevention of geological engineering under thermo-mechanical coupling working condition.

An inconsistent social buffering effect from a static visual substitute in horses (Equus caballus): A pilot study

Social buffering occurs when a companion helps to reduce a subject’s stress response during a stressful event and/or to recover more quickly from a stressful event. The aim of this study was to determine if any social buffering function of a horse companion can occur in relation to a visual substitute (poster of a relaxed horse face) during two different stressful situations: a novel object test (a ball presented gradually) and an umbrella test (sudden opening of an umbrella). In order to evaluate the effect of a horse face poster on the subject’s stress responses, behavioral (reactivity score) and physiological (heart rate) measurements were taken. Each of the 28 study subjects completed four trials: two novel object tests and two umbrella tests—each with a relaxed horse face poster and a pixelated poster (control). Our results showed that the presence of a horse face poster might significantly reduce the behavioral response (reactivity) of subject horses in the novel object test but did not improve heart rate recovery (HRR) time. The horse face poster did not have a significant effect on the behavioral reaction or HRR in the umbrella test. Any effect of a horse face poster as a potential social buffer might potentially depend on the nature of the stressful event.

COMPARATIVE ANALYSIS OF THE IMPACT OF DOMESTIC DEBT ON ECONOMIC GROWTH IN NIGERIA AND GHANA

The study compared Nigeria and Ghana's domestic debt (DM) and economic growth (EG) from 2000 to 2021.World Development Indicators, CBN Statistical Bulletin, Ghana Ministry of Finance Annual Debt Statistics, and CBN Annual Report provided secondary data (Time Series) for the study. The study examined Treasury Bills (TBL), Treasury Certificates (TCF), Treasury Bonds (TBD), Development Stock (DST), and Promissory Note (PNT) with one control variable, Interest Rate (INTR), in relation to EG proxied with Real Gross Domestic Product (RGDP) in Nigeria and Ghana. Descriptive statistics, correlation analysis, and multiple regression analysis were performed using E-VIEW version 9.0. The findings indicated The p-value of TBL is 0.4523 for Nigeria and 0.0039 for Ghana, which means that TBL has no effect on RGDP in Nigeria but a substantial effect in Ghana. TCF's p-values of 0.3003 and 0.0700 for Nigeria and Ghana, respectively, are more than 0.05, indicating that TCF has no significant effect on RGDP in Nigeria and Ghana. TBD's p-values for Nigeria and Ghana are 0.0000 and 0.5921, respectively, which is less than 0.05 for Nigeria but higher than 0.005 for Ghana, indicating that TBD has a substantial effect on RGDP in Nigeria but not in Ghana. DST has a substantial effect on RGDP in Nigeria but not in Ghana. Nigeria's p-value is 0.002, which is less than the specified value of 0.05, whereas Ghana's is 0.8106, which is greater than 0.005. Nigeria's p-value of 0.1622 is higher than the set value of 0.05, whereas Ghana's is less than 0.005, indicating that PNT has a substantial effect on RGDP in Ghana but not in Nigeria. INTR's p-values for Nigeria and Ghana are 0.5667 and 0.0371, respectively, which are more than 0.05 for Nigeria but less than 0.005 for Ghana, indicating that INTR has no effect on RGDP in Nigeria but a significant effect in Ghana. Finally, the study finds inconsistent effects of domestic factors on EG in Ghana and Nigeria. The government (GOVT) should borrow mostly from FGN bonds. The outcome shows that FGN bonds greatly benefit EG in Nigeria and Ghana.
 Keywords: Debts, Domestic, Economic, Growth and Bonds.

Exogenous silicon application improves fruit yield and quality of drip-irrigated greenhouse tomato by regulating physiological characteristics and growth under combined drought and salt stress

Improving crop productivity in drought-stressed and salt-affected regions is the key to promoting sustainable agriculture. Silicon is considered to improve the stress tolerance of crops, but how exogenous silicon application affects the fruit yield and quality of tomato under combined drought and salt stress remains unclear. A greenhouse experiment was conducted over two consecutive growing seasons (autumn 2021 and spring 2022) to investigate the effects of exogenous silicon application on leaf greenness, photosynthesis and chlorophyll fluorescence parameters, fruit yield and quality of drip-fertigated greenhouse tomato under various drought and salt stresses. There were three irrigation levels: I1 (90–100% θf, where θf is the field capacity), I2 (70%–80% θf) and I3 (50%–60% θf), two soil salinity levels: S0 (0.1%) and S1 (0.4%), and two silicon application rates: T0 (0 mmol L−1) and T1 (2.4 mmol L−1), resulting in a total of 12 treatments with three replicates. The results showed that the combined drought and salt stress significantly reduced leaf silicon content, plant height, leaf area index, aboveground biomass, net photosynthetic rate, transpiration rate and stomatal conductance of tomato plants, while the exogenous silicon application promoted these parameters. Irrigation level, salinity level, silicon rate and the interaction of irrigation level and salinity level had significant effects on fruit yield. Compared to S0, S1 decreased fruit yield by 18.42%, 17.91% and 20.64% under I1, I2 and I3, respectively. Exogenous silicon application significantly improved fruit yield by 6.52% (I3S0) - 17.73% (I2S1). Irrigation level, salinity level and silicon rate had inconsistent effects on fruit quality. Exogenous silicon application significantly increased total soluble solids, Vitamin C, soluble sugars and lycopene content, without significant effect on titratable acid. The lower irrigation level and higher salinity level resulted in better fruit quality. The path analysis showed that leaf silicon content, net photosynthetic rate, lycopene, average fruit weight had significant direct positive effects on fruit yield. The results are informative for improving drip-fertigated greenhouse tomato productivity and quality under drought-stressed and salt-affected conditions.

Effects of transcutaneous auricular vagus nerve stimulation on P300 magnitudes and salivary alpha-amylase during an auditory oddball task

Transcutaneous auricular vagus nerve stimulation (taVNS) is a non-invasive neurostimulation technique that is thought to modulate noradrenergic activity. Previous studies have demonstrated inconsistent effects of taVNS on noradrenergic activity, which is possibly due to insufficient statistical power, suboptimal stimulation parameter settings, and data collection procedures. In this preregistered within-subject experiment, 44 healthy participants received taVNS and sham (earlobe) stimulation during two separate experimental sessions. Stimulation intensity was individually calibrated to the maximum level below pain. During each session, participants received the stimulation continuously ten minutes before an auditory novelty oddball task till the end of the experimental session. The P3b component of the event-related potential served as a marker of phasic noradrenergic activity, whereas P3a magnitude was explored as an index of dopaminergic activity. Salivary alpha-amylase (sAA) was measured as an index of tonic noradrenergic activity before and at the end of the stimulation. The taVNS and sham conditions did not differ in P3a or P3b magnitudes, nor sAA secretion. These findings call into question whether taVNS, administered continuously at high, nonpainful stimulation intensities, reliably augments noradrenergic activity via the vagus nerve.

Profiles of diversity and inclusion motivation: Toward an employee‐centered understanding of why employees put effort into inclusion and exclusion

AbstractDespite their prevalence, workplace diversity and inclusion (D&I) practices continue to produce inconsistent effects on employee attitudes and behaviors. This shines a light on the need for evidence‐based approaches in understanding how employees enact D&I goals. Drawing on self‐determination theory, we argue that employee D&I motivation is an untapped pathway for understanding how inclusion‐supportive employee outcomes (i.e., more inclusion and less exclusion) emerge. In addition, we leverage attribution theory to inform when employee motivation can be shaped, demonstrating that employee attributions of the intentions behind their organization's D&I practices play an important role in shaping their subsequent D&I motivation. Across two studies, latent profile analysis identified five distinct profiles of D&I motivation, which were differentially related to inclusion and exclusion (Study 1) and predicted by D&I attributions (Studies 1 and 2). Combined, these findings highlight the importance of understanding employee motivations and attributions within the context of D&I. Such knowledge can inform how employees enact inclusion goals, which has the potential to help organizations realize the desired benefits of diversity.

Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding.

Non-alcoholic fatty liver disease (NAFLD) can progress to more severe stages, such as steatohepatitis and fibrosis. Thermoneutral housing together with high-fat diet promoted NAFLD progression in C57BL/6J mice. Due to possible differences in steatohepatitis development between different C57BL/6 substrains, we examined how thermoneutrality affects NAFLD progression in C57BL/6N mice. Male mice were fed standard or high-fat diet for 24 weeks and housed under standard (22°C) or thermoneutral (30°C) conditions. High-fat feeding promoted weight gain and hepatic steatosis, but the effect of thermoneutral environment was not evident. Liver expression of inflammatory markers was increased, with a modest and inconsistent effect of thermoneutral housing; however, histological scores of inflammation and fibrosis were generally low (<1.0), regardless of ambient temperature. In standard diet-fed mice, thermoneutrality increased weight gain, adiposity, and hepatic steatosis, accompanied by elevated de novo lipogenesis and changes in liver metabolome characterized by complex decreases in phospholipids and metabolites involved in urea cycle and oxidative stress defense. Thermoneutrality appears to promote NAFLD-associated phenotypes depending on the C57BL/6 substrain and/or the amount of dietary fat.

Modeling response of tree slenderness to climate, soil, diversity, and competition in natural secondary forests

The tree slenderness coefficient (TSC) serves as a simplified measure of tree stability, which is subject to the complex influences of climate, soil, diversity, and competition. Quantifying and exploiting the risks affecting the TSC facilitates the development of forest stability restoration measures. In the current climate change and multiple risk coexistence scenario, the interactions of risks are expected to have non-negligible effects on the TSC. However, past efforts have been limited to independent effects without interactive effects, which is detrimental to a proper understanding of ecology. For example, the inconsistent effect direction of the same indicator on TSC in past studies may be due to the neglect of the interactive effects of the indicators. Natural secondary forests are effective mitigation tools for climate change that urgently need to be managed appropriately to maximize their stability and provide a wide range of benefits. Therefore, we conducted the first comprehensive investigation of both independent and interactive effects of climate, soil, diversity, and competition on TSC, utilizing data from 13,874 trees of three principal tree species distributed in Chinese boreal natural secondary forests. Further, simulation examples were provided to present actionable management measures using the improved insight. The results showed that short-wave solar radiation (Srad), cation exchange capacity (CEC), Pielou evenness index (Pielou), and basal area (BA) exerted positive independent influences on TSC, while mean annual precipitation (MAP) did the opposite. The significant interaction terms were BA × Pielou, BA × Srad, Pielou × CEC, and Srad × CEC, all of which showed positive effects on TSC. In addition, the interactions produced non-negligible forces on the TSC, especially for small-sized trees, as the relative importance was quite large. Importantly, the effect directions of the indicators on the TSC were inconstant and changed with the gradients of other indicators due to the presence of interactions. Finally, the simulation results showed that tree stability in 97.1% of the plots was negatively affected by Srad or CEC, which could be transformed into positive effects by adjusting BA or Pielou above the critical points. This research offers a novel insight into the importance of interactions in credible TSC modeling and indicator analysis, which may hopefully serve as a beginning to guide forest management based on tree stability while faced with environmental change.

High below‐ground bud abundance increases ecosystem recovery from drought across arid and semiarid grasslands

Abstract Asexual reproduction plays a fundamental role in the structure, dynamics and persistence of perennial grasslands. Thus, assessing how asexual reproductive traits of plant communities respond to drought may be key for understanding grassland resistance to drought and recovery following drought. Here, we quantified three asexual reproductive traits (i.e. above‐ground tiller abundance, below‐ground bud abundance and the ratio of tillers to buds) during a 4‐year severe drought and a 2‐year drought recovery period in four grasslands that spanned an aridity gradient in northern China. We also assessed the relationship between these traits and the resistance and recovery of above‐ground net primary productivity (ANPP). We found that drought had limited and largely inconsistent effects on asexual reproduction among drought and recovery years and grasslands overall. Drought increased tiller abundance in the first treatment year and reduced bud banks by the fourth treatment year across grasslands. However, neither of the three asexual reproductive traits were correlated with drought resistance of ANPP. Drought legacies differed among the four grasslands with positive, negative and no legacies evident for the three asexual reproductive traits, and no clear relationship with aridity. Bud banks and tiller to bud ratio decreased and increased, respectively, in the first recovery year, but not in the second recovery year. In contrast to drought resistance, community bud abundance was strongly related to recovery, such that communities with higher bud abundance had greater ANPP recovery following drought. Synthesis. These results suggest that asexual reproductive traits may be important drivers of ecosystem recovery after drought, but that variable responses of these asexual reproduction traits during drought complicates predictions of overall grassland responses.

The Impact of Forecast Inconsistency and Probabilistic Forecasts on Users’ Trust and Decision-Making

Abstract When forecasts for a major weather event begin days in advance, updates may be more accurate but inconsistent with the original forecast. Evidence suggests that resulting inconsistency may reduce user trust. However, adding an uncertainty estimate to the forecast may attenuate any loss of trust due to forecast inconsistency, as has been shown with forecast inaccuracy. To evaluate this hypothesis, this experiment tested the impact on trust of adding probabilistic snow-accumulation forecasts to single-value forecasts in a series of original and revised forecast pairs (based on historical records) that varied in both consistency and accuracy. Participants rated their trust in the forecasts and used them to make school-closure decisions. One-half of the participants received single-value forecasts, and one-half also received the probability of 6 in. or more (decision threshold in the assigned task). As with previous research, forecast inaccuracy was detrimental to trust, although probabilistic forecasts attenuated the effect. Moreover, the inclusion of probabilistic forecasts allowed participants to make economically better decisions. Surprisingly, in this study inconsistency increased rather than decreased trust, perhaps because it alerted participants to uncertainty and led them to make more cautious decisions. Furthermore, the positive effect of inconsistency on trust was enhanced by the inclusion of probabilistic forecast. This work has important implications for practical settings, suggesting that both probabilistic forecasts and forecast inconsistency provide useful information to decision-makers. Therefore, members of the public may benefit from well-calibrated uncertainty estimates and newer, more reliable information. Significance Statement The purpose of this study was to clarify how explicit uncertainty information and forecast inconsistency impact trust and decision-making in the context of sequential forecasts from the same source. This is important because trust is critical for effective risk communication. In the absence of trust, people may not use available information and subsequently may put themselves and others at greater-than necessary risk. Our results suggest that updating forecasts when newer, more reliable information is available and providing reliable uncertainty estimates can support user trust and decision-making.

Lipid levels in the Jiarong Tibetan's diet at high altitudes: a cross-sectional survey.

Despite the ongoing debate on the inconsistent and controversial effects of Tibetan diet on blood lipid levels at high altitude, this cross-sectional study was conducted to analyze the relationship between dietary practices and blood lipid levels among Jiarong Tibetan population. A total of 476 Jiarong Tibetan residents were included, in which basic demographic data, physical activity records, simplified food frequency questionnaire, and biochemical data were collected. Using multivariate logistic regression analysis, the potential associations between the variables were examined, and it was found that fat energy supply ratio increased with the elevation of altitude, while the lipid level showed an inverted U-shaped variation. However, the findings suggested that a diet rich in unsaturated fatty acids might balance the effects of the Tibetan diet on the risk of lipid metabolism disorders. Therefore, it is crucial to concentrate on the fat composition rather than the amount of fat E% intake on the plateau. The results highlighted the importance of investigating the interaction between environment and genes in lipid levels among plateau Tibetan population. However, further large-scale prospective studies are required for better understanding of the complexities involved in dietary practices and their influences on blood lipid levels.

The Effects of Pubertal Timing on Academic Performance in Adolescence and Career Success in Adulthood: Evidence from a 16-year Longitudinal Study.

Previous research showed inconsistent effects of pubertal timing on adolescent academic performance and adult career success. Moreover, the relative importance of biological vs. perceived pubertal timing has not been examined. This study examined effects of biological and perceived pubertal timing on academic performance throughout adolescence and career success in adulthood together with sex differences in an understudied population of pre-dominantly Black youth from lower income families. The sample included 704 youth (52% male, 76% Black, 22% White) interviewed at four time points (Mean ages: 11.8, 13.2, 17.6, and 27.7 years). The results from a mediation path model showed that among males, perceived off-time pubertal timing uniquely predicted lower concurrent academic performance as well as lower objective career success in adulthood; this effect was mediated by lower academic performance throughout adolescence. Additionally, results from bivariate correlation analyses showed associations between early biological pubertal timing and lower concurrent academic performance in males and early perceived pubertal timing and lower concurrent academic performance among females. These findings contribute to the understanding of more nuanced links between pubertal timing, academic performance and subsequent career success in an understudied population of pre-dominantly Black youth from lower income families.

Exploring Acceptability of Employment Interventions to Support People Living With Cancer: Qualitative Study of Cancer Survivors, Health Care Providers, and Employers.

Employment contributes to cancer survivors' quality of life, but this population faces a variety of challenges when working during and after treatment. Factors associated with work outcomes among cancer survivors include disease and treatment status, work environment, and social support. While effective employment interventions have been developed in other clinical contexts, existing interventions have demonstrated inconsistent effectiveness in supporting cancer survivors at work. We conducted this study as a preliminary step toward program development for employment support among survivors at a rural comprehensive cancer center. We aimed (1) to identify supports and resources that stakeholders (cancer survivors, health care providers, and employers) suggest may help cancer survivors to maintain employment and (2) to describe stakeholders' views on the advantages and disadvantages of intervention delivery models that incorporate those supports and resources. We conducted a descriptive study collecting qualitative data from individual interviews and focus groups. Participants included adult cancer survivors, health care providers, and employers living or working in the Vermont-New Hampshire catchment area of the Dartmouth Cancer Center in Lebanon, New Hampshire. We grouped interview participants' recommended supports and resources into 4 intervention delivery models, which ranged on a continuum from less to more intensive to deliver. We then asked focus group participants to discuss the advantages and disadvantages of each of the 4 delivery models. Interview participants (n=45) included 23 cancer survivors, 17 health care providers, and 5 employers. Focus group participants (n=12) included 6 cancer survivors, 4 health care providers, and 2 employers. The four delivery models were (1) provision of educational materials, (2) individual consultation with cancer survivors, (3) joint consultation with both cancer survivors and their employers, and (4) peer support or advisory groups. Each participant type acknowledged the value of providing educational materials, which could be crafted to improve accommodation-related interactions between survivors and employers. Participants saw usefulness in individual consultation but expressed concern about the costs of program delivery and potential mismatches between consultant recommendations and the limits of what employers can provide. For joint consultation, employers liked being part of the solution and the possibility of enhanced communication. Potential drawbacks included additional logistical burden and its perceived generalizability to all types of workers and workplaces. Survivors and health care providers viewed the efficiency and potency of peer support as benefits of a peer advisory group but acknowledged the sensitivity of financial topics as a possible disadvantage of addressing work challenges in a group setting. The 3 participant groups identified both common and unique advantages and disadvantages of the 4 delivery models, reflecting varied barriers and facilitators to their potential implementation in practice. Theory-driven strategies to address implementation barriers should play a central role in further intervention development.

Inconsistent Effects of Glatiramer Acetate Treatment in the 5xFAD Mouse Model of Alzheimer's Disease.

Alzheimer's disease (AD) is a chronic neurodegenerative disorder that involves strong inflammatory components. Aberrant and prolonged inflammation in the CNS is thought to contribute to the development of the pathology. The use of single cytokine approaches to curb or leverage inflammatory mechanisms for disease modifying benefit has often resulted in conflicting data. Furthermore, these treatments were usually delivered locally into the CNS parenchyma, complicating translational efforts. To overcome these hurdles, we tested the use of glatiramer acetate (GA) in reducing amyloid beta (Aβ) plaque pathology in the 5xFAD model of AD. GA immunizations were begun at the ages of 2.5 months, 5.5 months, and 8.5 months, and GA was delivered weekly for 8 weeks. While previous data describe potential benefits of GA immunization in decreasing Aβ levels in murine models of AD, we found modest decreases in Aβ levels if given during the development of pathology but, surprisingly, found increased Aβ levels if GA was administered at later stages. The impact of GA treatment was only significant for female mice. Furthermore, we observed no changes between microglial uptake of plaque, CD11c immunopositivity of microglia, or levels of TMEM119 and P2Ry12 on microglia. Overall, these data warrant exercising caution when aiming to repurpose GA for AD.

Soil texture analysis by laser diffraction and sedimentation and sieving–method and instrument comparison with a focus on Nordic and Baltic forest soils

PurposeLaser diffraction (LD) for determination of particle size distribution (PSD) of the fine earth fraction appeared in the 1990s, partly substituting the Sieving and Sedimentation Method (SSM). Whereas previous comparison between the two methods predominantly encompasses agricultural soils, less attention has been given to forest soils, including pre-treatment requirements related to their highly variable contents of carbon and Alox+ Feox. In this small collaborative learning study we compared (1) national SSM results with one type/protocol of LD analysis (Coulter), (2) LD measurements performed on three different LD instruments / laboratories, and (3) the replication error for LD Coulter analysis of predominantly sandy and loamy forest soils.MethodsWe used forest soil samples from Denmark, Norway and Lithuania and their respective national SSM protocols / results. LD analyses were performed on Malvern Mastersizer 2000, Sympatec HELOS version 1999, and Coulter LS230, located at University of Copenhagen, Aarhus University and Helsinki University, respectively. The protocols differed between laboratories, including the use of external ultrasonication prior to LD analysis.ResultsThe clay and silt fractions content (&amp;lt;20 μm) from the LD analysis were not comparable with SSM results, with differences ranging from −0.5 to 22.3 percentage points (pp) for clay. Preliminary results from loamy samples with spodic material suggested inconsistent effects of external ultrasonication to disperse aggregates. The comparison between the three LD instruments showed a range in the clay and silt fractions content of 1.9–5.3 and 6.2–8.1 pp, respectively. Differences may be related to the instruments, protocols, and content of a given particle size fraction. The replication error of the Coulter LD protocol was found to be &amp;lt;3 pp in sandy soils, but up to 10 pp in loamy soils.ConclusionDifferences in the clay fraction results partly affected the classification of soil types. The fast replication of the LD analysis enables more quality control of results. The pedological evaluation of non-silicate constituents and optional pre-treatment steps (e.g., soil organic matter or sesquioxides) remains the same for LD and SSM. For comparison of results, detailed descriptions of the analytical protocol including pre-treatments are needed irrespective of instrument and theoretical approach.