The aim of this article is to determine the effect of the biaxial loading of a car seat, in vertical and horizontal directions, on seating comfort. We measured the transfer characteristic in the vertical direction with a free load. The method of laboratory testing of the car seat was chosen due to the simple repetition of the tests, ensuring the same test conditions, with high accuracy of their reproduction. To determine the effect, tests with uniaxial loading were compared with tests with biaxial loading. The resulting characteristic was the transfer characteristic in the vertical direction. The effect of horizontal loading on the transfer characteristic of a car seat was determined.