Soil is a non-renewable resource, and its degradation compromises human health, natural ecosystems, and even the climate. The application of organic amendments and herbicides is commonplace in agriculture, and their impact on soil fertility needs to be evaluated. Therefore, the objective was to evaluate the effect on soil microbial activity and structure of amendments, sewage sludge (SS) and green compost (GC), and the rate of herbicide pethoxamid applied (2, 10 and 50 mg kg−1). Herbicide dissipation kinetics, soil dehydrogenase activity (DHA) and the profile of phospholipid-derived fatty acids (PLFAs) extracted from the soil have been determined in unamended (S) and amended (S + SS and S + GC) soils. The dissipation curves of pethoxamid applied at the three rates closely fitted a single first order kinetics model in all the soils. The dissipation rate decreased with the rate applied in the order 2 mg kg−1 > 10 mg kg−1 > 50 mg kg−1 in unamended and amended soils. However, the half-life or time required for 50% dissipation (DT50) of pethoxamid was not significantly different in unamended and amended soils when applied at 2 and 10 mg kg−1, but it was lower in the amended soils than in the unamended one when the herbicide was applied at the highest rate. The highest DHA mean values were obtained in S + GC treated with pethoxamid at 2, 10 and 50 mg kg−1; however, DHA was lower in S + SS than in S. Peak DHA values were observed in S and S + GC soils treated with pethoxamid at 2 mg kg−1 at 15 days of incubation, and in S, S + GC and S + SS treated with the herbicide at 10 and 50 mg kg−1 at 35 days of incubation. These peak DHA values are close to 50% of herbicide dissipation. A statistical analysis of the PLFA results has revealed significant effects for sampling time in all the soils, for the pethoxamid rate, and for the interaction between time and pethoxamid rate only in S + GC and S + SS. The application of organic amendments to soil accelerated the dissipation of higher rates of pethoxamid compared to the unamended soil, which is important to prevent the herbicide's negative impacts on the soil microbial community.
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