Effect Of Hematocrit Levels Research Articles

Effect of Hematocrit Level on the Blood Flow through Stenosed Artery: A Theoretical Study

In the paper we have focused the effect of Hematocrit on flow parameters, such as resistance, frictional resistance, and skin friction due to stenosis in the artery taking blood as non-Newtonian fluid. It has been found that resistance increases with the increasing of hematocrit level and stenos is height. It is also shown that a skin-friction increase with increasing of stenosis height and decreases with the decreasing of stenosis height. Moreover the effect of stenosis shape parameter is observed on resistance and skin-friction due to different hematocrit level. Finally we compare our result graphically.

Effect of Hematocrit Level on the Blood Flow through Stenosed Artery: A Theoretical Study

Effect of Hematocrit Level on the Blood Flow through Stenosed Artery: A Theoretical Study

Clinical validation and implementation of a multiplexed immunosuppressant assay in dried blood spots by LC–MS/MS

BackgroundTherapeutic drug monitoring of immunosuppressive drugs is important in transplant patients. We developed and validated liquid chromatography–mass spectrometry (LC–MS/MS) assay for simultaneous quantitation of tacrolimus (TaC), sirolimus (SrL), and cyclosporin A (CsA) in dried blood spots (DBSs) to offer patients home sample collection, avoiding travel for blood draws. MethodsAfter extraction, samples were analyzed by LC–MS/MS in multiple reaction monitoring mode. ResultsThe assay was linear between 1.2–40ng/ml for TaC and SrL, and 30–1000ng/ml for CsA. Inter- and intra-assay CVs were ≤14.8% for all 3 drugs. This method correlated well with the existing clinical whole blood assay, with coefficients of determination >0.95 for all 3 drugs. DBS quality control samples were stable for at least 30days at −20, 4, and 25°C. Stability of patient DBS samples was at least 5days at temperatures up to 60°C, except for SrL where degradation was observed at 60°C within 24h. No effect of hematocrit level, blood spot volume or punch location was observed. ConclusionImmunosuppressant levels measured in DBS correlate with whole blood LC–MS/MS assay and may contribute to successful outcome of organ transplant and patient satisfaction.

Suitability Assessment of a New Bedside Interference-Free Glucose System for Use in Critical Care When Compared With Current Technology

We compared the analytical performance of a new point-of-care glucose meter specifically designed to automatically correct for hematocrit level and other interference factors with 3 commonly used glucose meters. We evaluated all 4 hospital-based glucose meter technologies for inaccuracy, imprecision, and analytical interferences likely to be encountered in patients with critical illness. In addition, we compared the performance of this new meter with one currently in use in our neurosurgical intensive care unit (ICU) to evaluate conformity to the International Organization for Standards 15197 criteria. Imprecisions, both within run and day to day, were evaluated on all the 4 glucose meters. Inaccuracy (bias) of the meters and analytical interference were evaluated by comparing results from whole blood specimens with results from plasma samples obtained from these whole blood specimens run on a hexokinase reference method. Analytical accuracy was assessed with glucose specimens spiked with different interference factors. Clinical accuracy was assessed on 95 whole blood specimens collected from patients admitted to a neurosurgical ICU ward. Imprecision and method correlation studies demonstrated slight differences in the degree to which the meters correlated with the hexokinase reference method. Paracetamol showed significant interference with 1 of the 4 meters tested. Ascorbate showed significant interference with 3 of the 4 meters. Hematocrit level also affected the correlation between whole blood and plasma hexokinase glucose results on 3 of the 4 meters tested. Assessment of clinical accuracy demonstrated that only 1 of the meters met the International Organization for Standards 15197 criteria when applied to neurosurgical ICU patients. Correlation with plasma hexokinase values and hematocrit level interference are the main variables that differentiate glucose meters. A meter that directly correlates with the plasma hexokinase glucose reference method over a wide range of glucose concentrations and minimizes the effects of hematocrit level provides more reliable data as an adjunct to intensive insulin therapy in patients with critical illness. Of the 2 meters studied in the neurosurgical ICU, only the StatStrip (Nova Biomedical Corporation, Waltham, Mass) demonstrated a total error that was within the acceptable limits.

Differences in Glucose Values Obtained From Point-of-Care Glucose Meters and Laboratory Analysis in Critically Ill Patients

Blood for glucose analysis is often obtained interchangeably from indwelling catheters and fingersticks. To determine the level of agreement between glucose values obtained by laboratory analysis and with a point-of-care device for blood from 2 different sources: fingerstick and a central venous catheter. A method-comparison design was used. Point-of-care values for blood from fingersticks and catheters were compared with laboratory values for blood from catheters in a convenience sample of 67 critically ill patients. The effects of hematocrit level and finger edema on differences in glucose values between the 2 methods were also evaluated. A t test was used to determine differences in glucose values obtained via the 2 methods. Differences and limits of agreement were also calculated. Laboratory glucose values for blood from a catheter differed significantly from point-of-care values for blood from the catheter (t(1,66) = -9.18; P < .001) and from a fingerstick (t(1,66) = 6.53; P < .001). Glucose values for the 2 methods differed by 20 mg/dL or more for 1 of 6 patients (15%) for catheter samples and for 1 of 5 (21%) for fingerstick samples. Point-of-care glucose values for fingerstick and catheter samples did not differ (P = .98). Hematocrit level significantly explained the difference in glucose values between the 2 methods for both catheter (R(2) = 0.288; P < .001) and fingerstick (R(2) = 0.280; P = .02) samples. Use of a commonly used point-of-care device when precise glucose values are needed may lead to faulty treatment decisions.