Effects Of He-Ne Laser Irradiation Research Articles

The Effect of He-Ne laser Radiation and Temperature of Blood in Vitro on the Erythrocytes Sedimentation Rate Values in Healthy People

Background : The aim of this study is to research the in vitro effect of  the He-Ne laser radiation and study the effect of environmental temperature on Erythrocyte Sedimentation Rate (ESR) in blood samples from healthy . Objective : To show the effect of He-Ne laser and effect of environmental temperature on Erythrocyte Sedimentation Rate (ESR) Subject & Methods : He-Ne laser wave length of 632.8ηm was used for irradiation with 0.5mm diameter beam spot on blood samples, with power density  1mw/cm2 , The irradiation times were (1hr ) , The samples of blood were obtained  30 contributors and each sample was separated into three samples for irradiation and control . Results : The results showed that there were significant differences in the values of sedimentation rates for both class (25 -45 ) 0c degree heat compared with the values of sedimentation 25 0c degree heat temperature for irradiation and control . It shows the effect of temperature factor on the red blood cells sedimentation rate in blood samples from healthy people and see the effect of the degree of laboratory heat due to lack of efficient heating and cooling devices in the summer and winter on the sedimentation rates of values to be taken into consideration with the results . Conclusion :The change of temperature (high and low) has a great effect on the deposition and precipitation of red blood cells in health people and thus must be taken into consideration and this effect is also observed with the use of He - Ne laser at a temperature of 25 ° C.

WITHDRAWN: Effect of He-Ne laser irradiation on embryonic development in chicken eggs

This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). These articles are retracted at the request of the authors. The joint Editors-in-Chief agree with this decision.

Statistical Analysis of Instantaneous Effect of Polarized He-Ne Laser Radiation on the Elastic Modulus of Soft Biological Tissue

Introduction: This work is dedicated to investigating the effect of applying polarized He-Ne laser beam (λ= 632 nm, 10 mW, 1 j/cm2) with polarization direction perpendicular to the stress direction on the tensile strength of biological tissue (elasticity index of the Pleura 10*4*0.1 mm). Method and Material: The optimized characteristics of the laser illumination in the minimum times of radiation is detected here through some series of designed experiments and the results are completely applicable to the new investigations. To achieve the purpose, the experiments are designed in full factorial design model to study the effecting factor of viscoelastic modules of the pleura. The employed factor is time duration of the exposure. different irradiation timing is 5 s, 10 s, 15 s and 20 s. Result: Through the variance analysis it is found that time duration of radiation plays very important role on its viscoelastic characteristics. Additionally, it is revealed that a special condition, the time of exposure =10 sec, leads to the highest value of relative elastic modulus, i.e., the most increase in the tensile strength of the Pleura because of dehydration of cells is happened in longer time irradiation than 10s and no effect in less duration than 10s. Conclusion: This conclusion can be useful in dealing with wound scars, reforming the shape of the cornea, cartilage reshaping and et.

Characterization of miRNAs and their target genes in He-Ne laser pretreated wheat seedlings exposed to drought stress

Drought stress is considered a critical environmental factor that negatively affects wheat growth and development, which causes considerable losses in wheat yields worldwide. More recently, numerous microRNAs (miRNAs) have been found to be involved in wheat responses to drought stresses. However, there is little information regarding the effects of He-Ne laser irradiation on the expression traits of miRNAs and their targets in wheat seedlings exposed to drought stress. In the current study, therefore, a combination of physiological and molecular approaches was used to assess the effect of He-Ne laser irradiation on the expression of miRNAs and their targets in wheat seedlings exposed to drought stress. Our results demonstrated that drought stress significantly reduced plant height, root length, shoot and root fresh weight, relative water content, the expression level and activity of superoxide dismutase (SOD), enhanced malondialdehyde (MDA) concentration in the wheat seedlings. However, He-Ne laser irradiation significantly enhanced the activities of SOD, ascorbate peroxidase (APX), peroxidase (POD) and relative water content, and reduced MDA concentration of seedlings by regulating gene expression for SOD, POD, APX. In addition, in comparison with drought stress alone, miR160, miR164 and miR398 transcripts were down-regulated, and expression levels of its targets auxin response factor (ARF22), NAC domain transcription factor and Cu/Zn superoxide dismutases (CSD) were up-regulated in He-Ne laser irradiated seedlings exposed to drought stress. These results suggested that He-Ne laser irradiation could possible protection of drought stress, at least partially, by increasing the transcript levels and activities of SOD, POD and APX, and decreasing the transcript levels of miR160, miR164 and miR398. To the best of our knowledge, this is the first study to present biochemical and molecular evidence supporting the effect of He-Ne laser irradiation on the alleviation of drought stress in wheat seedlings mediated by miRNA expression.

The Effect of He-Ne and Diode Lasers on the Electrical Characteristics of Silicon Diode

The effect of He-Ne (632.8 nm) and diode (650 nm) lasers on the electrical characteristics of silicon diode have been studied. The electrical characteristics of the diode were recorded before laser irradiations, then the diode is subjected to He-Ne laser for 5 and 10 minutes and then the diode electrical characteristics were recorded for each time of exposure and the same was done in the case of irradiation with diode laser. The electrical characteristics of the diode before and after laser irradiations were compared and thermal effect was noticed when compared the effect of lasers irradiation and the well known temperature effect on the electrical characteristics of the diode. It was found that the effect of the He-Ne (632.8 nm) and diode laser (650 nm) on the electrical characteristics of silicon diode at exposure time of 5 minutes were comparable but for 10 minutes of exposure the effect of He-Ne laser irradiation on the characteristics was different from that of diode laser and this is due to the fact that the two lasers has different properties.

Effects of acupoint laser irradiation on gastric acid secretion and NO content in chronic atrophic gastritis

Objective To study the effects of He-Ne laser irradiation on the pH value of gastric juice, the ultrastructure of gastric parietal cells and the content of nitric oxide (NO) in chronic atrophic gastritis (CAG). Methods Sixty healthy male Wistar rats were randomly divided into a normal control group, a model group, a drug group, an acupuncture group and a laser acupoint group, each of 12. A CAG model was successfully introduced to all except those in the normal control group through the administration of sodium salicylate and alcohol, combined with fasting and water deprivation, running fatigue and other interventions. The rats of the drug, acupuncture and laser acupuncture groups were given medications, acupuncture at Zusanli acupoint and laser irradiation at Zusanli acupoint accordingly. After 14 days of treatment, all of the rats were executed and diluted gastric juice and gastric tissues were collected. NO content in serum was determined using a nitrate enzyme reduction method, the pH value of gastric juice was measured, and the ultrastructure of the gastric mucosa was observed using a transmission electron microscope. Results The average pH of the gastric juice and the average serum NO content were both significantly higher in the model group than in the normal control group. In the model group the membrane structure of the parietal cells was absent or partly dissolved and the mitochondria were swollen. Drugs, acupuncture and laser irradiating at the Zusanli acupoint all corresponded with reduced pH and NO content, but the most significant improvements were observed in the laser acupuncture group. In the laser acupuncture group the membrane structure of parietal cells was complete, and the cytoplasmic organelles had nearly returned to normal. Conclusions Laser irradiation can promote recovery from CAG, increasing the secretion of gastric acid and decreasing the serum level of NO. Both have a good curative effect for CAG. Laser irradiation deserves further clinical research and promotion. Key words: Laser acupuncture; Lasers; Acupuncture; Gastritis; Nitric oxide

The detoxification effects of He-Ne laser irradiation on cytotoxicity of cadmium sulfide nanoparticles (CdSNPs) in tall fescue seedlings

Because of their wide applications in manufacturing life science and medicine, nanoparticles (NPs) are becoming potential limiting factors for agricultural plant growth and productivity. Thus, deve...

Cell wall reconstruction and DNA damage repair play a key role in the improved salt tolerance effects of He-Ne laser irradiation in tall fescue seedlings.

The improved salt tolerance effects of He-Ne laser were further studied through the estimation of ROS levels, cell viability, DNA damage phenomena, physicochemical properties, and monosaccharide compositions of cell wall polysaccharides in tall fescue seedlings. Salt stress produced deleterious effects on seedlings growth and development. ROS levels and genomic DNA damage were markedly increased compared with controls. Physicochemical activities and monosaccharide proportions of cell wall polysaccharide were also pronouncedly altered. He-Ne laser irradiation improved plant growth retardation via increasing cell viability and reverting physicochemical parameters. According to the results of Fourier transform infrared (FTIR) scanning spectra and DNA apopladder analysis, He-Ne laser was showed to efficiently ameliorate cell wall polysaccharide damage and DNA fragmentation phenomena. The treatment with DNA synthesis inhibitor further demonstrated that DNA damage repair was correlated with the improvement effects of the laser. Therefore, our data illustrated that He-Ne laser irradiation resulted in cell wall reconstruction and genomic DNA injury repair in vivo in salt-stressed seedlings, then enhanced salt tolerance probably via interactions between plant cell wall and related resistance gene expression pattern.

Effect of low energy He-Ne laser radiation on microtubule-associated protein 2 expression in cultured rat hippocampal neurons

Objective To study the effect of low energy He-Ne laser radiation on microtubule-associated protein 2 (MAP2) expression in cultured rat hippocampal neurons.Methods Neonatal rat hippocampal neurons were isolated and cultured in vitro.After 14 days culture with or without low energy He-Ne laser radiation,neurons were taken out; MAP2 expression was shown by using immunohistochemistry method and MAP2-positive neurons were counted on the photographs taken under light microscope.Results The amount of MAP2-positive neurons in laser radiation group was (15.85 ± 2.79)/high power field and was much higher than that in control group (9.23 ± 2.56).Positive expression neurons had clear contours.The difference of MAP2 expression between laser radiation group and control group was statistically significant (P ＜ 0.01).Expression of MAP2 was up-regulated after laser radiation in cultured neurons.Conclusions He-Ne laser radiation may up-regulate the expression of MAP2 in cultured neurons. Key words: He-Ne laser; Cultured neuron, in vitro; Microtubule-associated protein 2

HeNe laser increases the pro-inflammatory cytokines production and the in vitro phagocytosis of P. brasiliensis by macrophages.

Event Abstract Back to Event HeNe laser increases the pro-inflammatory cytokines production and the in vitro phagocytosis of P. brasiliensis by macrophages. Thiago Alves Da Costa1, Rodolfo Thomé1, Rosaria Di Gangi1, Carolina Francelin1 and Liana Verinaud1* 1 State University of Campinas, Department of Structural and Functional Biology; Institute of Biology., Brazil Cutaneous lesions caused by the fungus Paracoccidioides brasiliensis (Pb) lead to painful ulcerative wounds, which are difficult to be cured by conventional treatment with anti-fungal drugs due to their adverse effects and the multidrug resistance that some fungal isolates present (Andriole, 1998, Trent & Kirsner, 2003). We have previously showed that Helium-Neon (HeNe) laser irradiation of paracoccidioidomycotic lesions in mice accelerates the wound healing as well as the fungal clearance, which is very probably linked to the death of the fungus by macrophages (Nagib et al., 2010, Alves da Costa et al., 2013). In this study we evaluated, in vitro, whether HeNe laser irradiation was capable of modulating the phenotypic and functional profile of human and murine macrophages. Peritoneal macrophages from BALB/c mice were irradiated with different dosages of HeNe laser (0.836; 1.5 and 3J/cm2) and cultivated with Pb yeast cells (1:4, macrophage:fungi) for 6 hours. Flow cytometry analysis of macrophages showed that laser irradiation promoted an increase in the expression of TLR-4, which are molecules involved in yeast recognition and internalization, but did not alter other markers, like CD11b, F4/80, MHC-II, for example. Also, co-cultures of irradiated macrophages and Pb showed a higher phagocytic index and a higher pro-inflammatory (IFN-γ, IL-6, TNF-α) cytokines production. Similar results were obtained with a human macrophage cell line (THP1) even under the lowest incidence energy of the laser. Collectively, our results indicate that the accelerated wound healing process, observed after the treatment of paracoccidioidomycotic lesions with the HeNe laser, must be associated with a direct action of irradiation on macrophages. Acknowledgements Supported by FAPESP (#2010/08485-5, #2012/22131-7) and CAPES. References Alves da Costa, T., Di Gangi, R., Costa, M. R. S. N., Thomé, R., Paulino, L. C., Verinaud, L. (2013) . Effect of Helium-Neon (HeNe) laser irradiation on lesions in experimental paracoccidioidomycosis. Trends In Photochemistry And Photobiology, Accepted manuscript. Andriole, V. T. (1999). The 1998 Garrod lecture. Current and future antifungal therapy: new targets for antifungal agents. J. Antimicrobial Chemotherapy 44, 151-162. Nagib, P. R. N., Gameiro, J., Alves da Costa, T., Di Gangi, R., Ribeiro, J. S., Paulino, L. C., Verinaud, L. (2010). Effect of HeNe laser irradiation on extracellular matrix deposition and expression of cytokines and chemokines in paracoccidioidomycotic lesions. Photochem. Photobiol. 86, 920-924. Trent, J. T., Kirsner, R. S. (2003). Identifying and treating mycotic skin infections. Adv Skin Wound Care 16, 122-9. Keywords: Paracoccidioides brasiliensis, paracoccidioidomycotic lesions, Phagocytosis, Pro-inflammatory cytokines, HeNe Laser Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Alves Da Costa T, Thomé R, Di Gangi R, Francelin C and Verinaud L (2013). HeNe laser increases the pro-inflammatory cytokines production and the in vitro phagocytosis of P. brasiliensis by macrophages.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01003 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Liana Verinaud, State University of Campinas, Department of Structural and Functional Biology; Institute of Biology., Campinas, SP, Brazil, verinaud@unicamp.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Thiago Alves Da Costa Rodolfo Thomé Rosaria Di Gangi Carolina Francelin Liana Verinaud Google Thiago Alves Da Costa Rodolfo Thomé Rosaria Di Gangi Carolina Francelin Liana Verinaud Google Scholar Thiago Alves Da Costa Rodolfo Thomé Rosaria Di Gangi Carolina Francelin Liana Verinaud PubMed Thiago Alves Da Costa Rodolfo Thomé Rosaria Di Gangi Carolina Francelin Liana Verinaud Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Effects of He-Ne laser irradiation on the expression of proliferating cell nuclear antigen and B cell lymphoma/leukemia-2 protein in the gastric mucosa of rats with chronic atrophic gastritis

Objective To study the effects of He-Ne laser irradiation on histological changes and the expression of proliferating cell nuclear antigen ( PCNA ) and B-cell lymphoma/leukemia-2 ( Bcl-2 ) in the gastric mucosa of rats with chronic atrophic gastritis (CAG) so as to elucidate the relationship of He-Ne laser irradiation with precancerous lesions and apoptosis in the gastric mucosa. Methods The rats were divided into a normal group,a model group and a laser group.A model of CAG was established by gastric perfusion with a mixture of sodium salicylate and alcohol combined with irregular fasting and forced exercise.A He-Ne laser was used to irradiate the rats at 3.36 J/cm2 for 7 min daily for 20 d.Histopathological changes including the severity of inflammation in the gastric mucosa and the morphology and structure of the parietal cells were observed with a light microscope,and the expression of PCNA and Bcl-2 was detected with immunohistochemical methods. Results The pathologic morphological changes in the gastric mucosa of the model group were atrophy of the glands of the gastric mucosa and notable inflammatory infiltration.But in the laser group inflamed cells decreased,and the morphology,structure and volume of the cells all recovered close to normal.The immunohistochemistry results showed that during the atrophy of the gastric mucosa the expression of PCNA and Bcl-2 was elevated,and it was significantly higher in the model group than in the normal group.After irradiation the expression of PCNA and Bcl-2 was significantly lower. Conclusions There was hyper-proliferation in the gastric mucosa of the CAG model rats,with high expression of apoptosis suppressor PCNA and Bcl-2 proteins.Laser irradiation can reduce the expression of PCNA and Bcl-2,enhance cell proliferation and induce apoptosis,preventing the development of cancer.Laser irradiation has a good adjuvant therapeutic effect for all the pathological changes observed. Key words: Chronic atrophic gastritis; He-Ne lasers; Proliferating cell nuclear antigen; B-cell lymphoma/leukemia-2 ; Precancerous lesions

氦氖激光照射对大鼠实验性牙移动牙周组织中环氧化酶-2表达的影响

氦氖激光照射对大鼠实验性牙移动牙周组织中环氧化酶-2表达的影响

Effects of He-Ne laser irradiation on the storage of turkey semen

Maintenance or improvement of sperm quality during storage could prevent the loss of fertilizing capacity associated with stored turkey semen. Therefore the optimization of stored turkey semen could be useful to breeder industry since the commercial production of this bird relies almost entirely on artificial insemination. Previous research have shown that He-Ne laser irradiation in mammalian sperm increased the motility (Stato, 1986), decreased the mortality, promoted the acrosome reaction, which have a pivotal role in assisted fecundating programmes as therapy for resolving infertility in domestic animals

Effect of HeNe Laser Irradiation on Extracellular Matrix Deposition and Expression of Cytokines and Chemokines in Paracoccidioidomycotic Lesions

Paracoccidioidomycosis is the most prevalent human mycosis in Latin America. Cutaneous lesions are extremely painful and sensitive, and current treatment with antifungal drugs is lengthy and may cause side effects to patients. In this perspective, the helium-neon (HeNe) laser emerges as a novel therapy form due to its ability to heal wounds without changing cell function. In this work, we evaluate the effects of HeNe laser irradiation on extracellular matrix deposition and expression of cytokines and chemokines in cutaneous lesions caused by experimental infection of Balb/c mice. Our results showed decreased levels of pro-inflammatory interleukin (IL)-17 and tumor necrosis factor-alpha, and of anti-inflammatory IL-10 cytokines in lesions exposed to HeNe laser irradiation. Chemokines CCL3 and CXCL10 showed decreased levels in laser-treated lesions, but no significant difference was observed in relation to CCL5 expression. We also detected decreased density of fibronectin and laminin in HeNe laser-treated lesions. Data presented herein support the validity of our previous results suggesting positive effects of HeNe laser in accelerating wound healing in this experimental model. We believe that HeNe laser is a new nonharmful strategy that may be used as adjuvant and/or alternative therapy for improving treatment of paracoccidioidomycotic lesions.

Effect of He-Ne laser radiation on healing of osteochondral defect in rabbit: A histological study

This study examined the influence of low-level laser therapy (LLLT) on the healing of a large osteochondral defect in rabbits.An osteochondral defect with 5 mm diameter was surgically induced in the right femoral patellar groove of 48 adult male rabbits. They were divided into a control and an experimental group. The rabbits were treated at 2, 4, 8, and 16 weeks after surgery, with six rabbits in each study period being tested at each biweekly period. The experimental group received LLLT with a helium-neon (He-Ne) laser (632.8 nm, 10 mW) of 148.4 J/cm(2) three times a week, and the control group received placebo LLLT with equipment switched off. The defects were examined macroscopically and microscopically. The results of the histological examination 2 weeks after surgery showed that the osteochondral healing of the control group was significantly accelerated compared with that of the experimental group. However, the osteochondral healing of the experimental group 4 weeks and 16 weeks after surgery showed that healing accelerated significantly compared with that of the control group. The conclusion was that LLLT with an He-Ne laser could not significantly accelerate healing of a large osteochondral defect in rabbits of the experimental group compared with that of the control group throughout the duration of the present study.

氦氖激光照射对兔正畸牙周组织中血管内皮细胞生长因子受体-2表达的影响

氦氖激光照射对兔正畸牙周组织中血管内皮细胞生长因子受体-2表达的影响

Low-Energy Helium-Neon Laser Induces Locomotion of the Immature Melanoblasts and Promotes Melanogenesis of the More Differentiated Melanoblasts: Recapitulation of Vitiligo Repigmentation In Vitro

Helium-neon laser (He-Ne Laser, 632.8 nm) is a low-energy laser that has therapeutic efficacy on various clinical conditions. Our previous study has demonstrated efficacy of He-Ne laser on vitiligo, a disease characterized by skin depigmentation. To regain skin tone on vitiligo lesions, the process began by the migration of the immature melanoblasts (MBs) to the epidermis, which was followed by their functional development to produce melanin. In this study, we investigated the physiologic effects of He-Ne laser irradiation on two MB cell lines: the immature NCCmelb4 and the more differentiated NCCmelan5. The intricate interactions between MBs with their innate extracelluar matrix, fibronectin, were also addressed. Our results showed that He-Ne laser irradiation enhanced NCCmelb4 mobility via enhanced phosphorylated focal adhesion kinase expression and promoted melanogenesis in NCCmelan5. In addition, He-Ne laser decreased the affinity between NCCmelb4 and fibronectin, whereas the attachment of NCCmelan5 to fibronectin increased. The alpha5beta1 integrin expression on NCCmelb4 cells was enhanced by He-Ne laser. In conclusion, we have demonstrated that He-Ne laser induced different physiologic changes on MBs at different maturation stages and recapitulated the early events during vitiligo repigmentation process brought upon by He-Ne laser in vitro.

Effects of HeNe laser irradiation on experimental paracoccidioidomycotic lesions

Paracoccidioidomycosis (PCM) is the most prevalent human mycosis in Latin America. The infection is thought to take place firstly in the lungs and then may disseminate to other organs and tissues. Treatment by currently available antifungals is lengthy, the drugs may have undesirable side effects, and some are costly. Occasional resistant strains of Paracoccidioides brasiliensis, the causative agent of PCM, have been reported. So, the search for more efficient treatments or adjuvant therapies has to be continued. In this work, we evaluated the effects of HeNe laser irradiation on cutaneous inflammatory lesions caused by the inoculation of 5 × 10 6/0.1 ml yeasts cells into the back footpad of Balb/c mice. HeNe irradiation ( λ = 632.8 nm, 3 mW, incident energy of 3 J/cm 2) was applied at days 7, 8 and 9 post-infection and histological and immunohistochemical analysis were done. Unirradiated animals were used as controls. The results showed that laser-treated mice presented reduction of footpad edema, faster cutaneous wound healing, confluent granuloma, diffuse- and more loosely distributed immunolabeling for TNF-α, enhanced labeling of IFN-γ and any P. brasiliensis form detected, whereas multiple viable fungi were seen in diffuse widespread granulomas obtained from non-treated mice foot-pad. Fungi that were harvested from laser-treated animals presented no capability of growth in vitro as compared to those obtained from non-treated mice. We conclude that HeNe laser irradiation was able to inhibit the progress of inflammatory local reaction produced by P. brasiliensis infection and influence local cytokines production. We suggest that this treatment modality can be a useful coadjuvant tool to be combined with antifungal agents in the treatment of PCM ulcerations. The mechanisms involved in laser therapy of PCM lesions need further investigation.

Effects of He-Ne laser irradiation on chronic atrophic gastritis in rats

To study the effects of He-Ne laser irradiation on experimental chronic atrophic gastritis (CAG) in rats. Sixty-three male adult Wistar rats were randomly divided into five groups including normal control group, model control group and three different dosages He-Ne laser groups. The chronic atrophic gastritis (CAG) model in rats was made by pouring medicine which was a kind of mixed liquor including 2% sodium salicylate and 30% alcohol down the throat for 8 wk to stimulate rat gastric mucosa, combining with irregular fasting and compulsive sporting as pathogenic factors; 3.36, 4.80, and 6.24 J/cm(2) doses of He-Ne laser were used, respectively for three different treatment groups, once a day for 20 d. The pH value of diluted gastric acid was determined by acidimeter, the histopathological changes such as the inflammatory degrees in gastric mucosa, the morphology and structure of parietal cells were observed, and the thickness of mucosa was measured by micrometer under optical microscope. In model control group, the secretion of gastric acid was little, pathologic morphological changes in gastric mucosa such as thinner mucous, atrophic glands, notable inflammatory infiltration were found. After 3.36 J/cm(2) dose of He-Ne laser treatment for 20 d, the secretion of gastric acid was increased (P<0.05), the thickness of gastric mucosa was significantly thicker than that in model control group (P<0.01), the gastric mucosal inflammation cells were decreased (P<0.05). Morphology, structure and volume of the parietal cells all recuperated or were closed to normal. 3.36 J/cm(2) dose of He-Ne laser has a significant effect on CAG in rats.

In Vitro Effects of Helium-Neon Laser Irradiation on Human Blood: Blood Viscosity and Deformability of Erythrocytes

The purpose of this study was to investigate the in vitro effects of He-Ne laser irradiation on some rheological factors of human blood, such as blood viscosity, erythrocyte deformability, and sedimentation rate. The intravascular irradiation of low power laser has been applied in pre-clinical and clinical to treat various pathological processes. However, the mechanism is not fully understood so far. Especially the interaction and related mechanism between the laser and blood are unclear. In this work, by measuring the change of the main rheological factors after laser irradiation, the interaction and mechanism were explored. A30-mW He-Ne laser was used for irradiation with a 4-5-mm-diameter beam spot on blood samples, with a fluence rate of about 150 mW/cm.(2) The irradiation time was 60 min, so the total dose of irradiation was 540 J/cm.(2) The pathological samples of blood were obtained from patients (volunteers), and each sample was divided into two tubes for irradiation and control. The blood viscosity, erythrocyte deformability, and sedimentation rate were measured after laser irradiation and compared with un-irradiated control. The blood samples with poor erythrocyte deformability were prepared by adding Ca(2+) to the normal erythrocytes of a healthy person for investigating the laser effect on erythrocyte deformability further. Laser irradiation reduced the erythrocyte sedimentation rate of blood samples, which had a hyper-sedimentation rate originally. The blood viscosity of samples in hyper-values was lowered by laser irradiation in all shear rates measured (10-110 S(-1)), with a relative variation of approximately 10%. The deformability of erythrocytes from pathological samples and Ca(2+)-treated samples was improved after laser irradiation. The positive effects of laser irradiation on improving the rheological properties of blood were demonstrated in vitro.