Although hands-on activities significantly improve achievement and attitudes toward animals, the use of the aesthetically unpleasant is questionable. We investigated whether the use of woodlouse, as an example of an unpopular animal, alters children’s conservation attitudes, disgust for and knowledge of woodlice. The experimental group (n = 116), but not the control group (n = 110), achieved a better woodlouse conservation score with hands-on activities, but the intentions for woodlouse conservation were not generalized for conserving other animals. Disgust for woodlice was not influenced by the treatment, but females, albeit more disgust sensitive than men, showed higher conservation scores than men. Woodlouse knowledge scores significantly increased in both groups. In summary, this study demonstrates both the benefits and limits of using animals which are aesthetically unpopular, but essential parts of biodiversity and food chains.