The chemical characteristics of atmospheric pressure dielectric barrier discharge plasma generated in air are very sensitive to some factors, such as environment temperature and discharge power. There are two typical modes, namely the ozone mode and nitrogen oxides mode. In this paper, the air surface micro-discharge plasma in the ozone mode and nitrogen oxides mode are respectively generated at environment temperatures of 5 °C and 50 °C when discharge power is almost kept at 6 W, and the plasma-activated water (PAW) is prepared by means of passing the plasma exhaust gases into deionized water. By comparing the reactive species in PAW under these two modes and their sterilization effects, it is found that the concentrations of H+, H2O2, NO2 −, NO3 − and ONOO− in PAW for the nitrogen oxides mode are about 2–3 fold over those for the ozone mode, while the concentration of O3 in PAW for the ozone mode is more than 28 times that in the nitrogen oxides mode. The sterilization effect under the nitrogen oxides mode is two orders of magnitude higher than that in the ozone mode. For the biological effects, it is found that treatment of a methicillin-resistant Staphylococcus aureus suspension by PAW leads to approximately a four-log reduction in the nitrogen oxides mode, which is higher than that in the ozone mode, leading to speculations that the reactive nitrogen species represented by ONOO− in PAW may be the critical species in sterilization. Furthermore, the transition between ozone mode and nitrogen oxides mode can be achieved by adjusting the discharge power. Interestingly, both of the compositions and concentrations of the reactive species in gas and liquid phases under 5 °C & 9.5 W and 50 °C & 6 W conditions are roughly identical, indicating that the effect of environment temperature on the mode transition is consistent with that of discharge power.
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