Effect Of Different Seasons Research Articles

Effect of Different Seasons and Development Stages on the Chemical Composition and Bioactive Potential of Cardoon.

Cynara cardunculus L. (cardoon) is a wild species of the Mediterranean basin and is highly appreciated due to its rich nutritional value and versatile industrial applications. It is widely known that environmental conditions, such as air temperature, humidity, and solar radiation, among others, play a crucial role in plant phenological variations and the chemical composition and bioactive properties of different plant tissues of cardoon. This study applied several statistical methods to uncover the variations in biomolecules of different cardoon tissues collected in Greece over the growth cycle. The influence of the different seasons on the species is evident, resulting in a clear discrimination between the samples harvested throughout the growth cycle. In addition, the observed fluctuations in chemical composition are consistent with each vegetable tissue's functions and the plant's different physiological processes. This work allows for a better understanding and knowledge of the species, encouraging more profitable and sustainable use of all the plant parts.

Potential human health risk assessment associated with Hg, Cd, Pb, and As in sardines and shrimp from four Egyptian coastal governorates

Heavy metal contamination in seafood is a developing concern due to the potential negative consequences on human health. Egypt's coastal regions are important for seafood production and consumption, making it critical to assess the safety of these aquatic resources. The current study examined toxic metal levels (Hg, Pb, Cd, and AS) in 96 samples of sardine and shrimp from four Egyptian coastal governorates (Alexandria, Kafr El-Sheikh, Damietta, and Port Said) from 2019 to 2021. Inductively coupled plasma-optical emission spectroscopy (ICP-OES) was used to investigate the four hazardous metals. The recovery percentages of the determined metals ranged between 97 % and 99 %. Limits of detection (LOD) and limit of quantification (LOQ) for the determined metals ranged from 0.001 to 0.0077 mg/l and from 0.0035 to 0.026 mg/l, respectively. Mercury (Hg) was not found in any of the samples tested. The concentrations of Pb and Cd in the sardines and shrimp samples were higher in the winter seasons than in the summer seasons. Meanwhile, the difference in seasons had no effect on the concentration of As in the sardines and shrimp samples. The highest concentrations of the other three metals in the sardine and shrimp samples were used to calculate the Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), and Hazard Index (HI). The obtained THQ as well as the HI of Pb, Cd, and As were all less than 1.0 (with the exception of sardine samples from Kafr El-Sheikh Governorate, which recorded 1.262), indicating that there is no significant health risk to the consumer from consuming such sardines and shrimp from these governorates. The effect of different seasons on the concentrations of the metals under study does not have a specific behavior, but varies according to the governorate, the type of sample, and the type of contaminated metal. Due to the high level of contamination with heavy metals in sardine samples collected from Kafr El-Sheikh governorate (TTHQ = 1.26), an environmental study is required to determine the causes of contamination and control them.

Cryopreservation alters buffalo sperm kinematics and mitochondrial parameters, acrosome and intra-cellular calcium status

BACKGROUND: Little is known about the effects of different seasons on the cryopreservation success of buffalo sperm in terms of kinematics and sperm functional parameters. OBJECTIVE: To study the effect of three seasons (winter, comfort and summer) and cryopreservation on sperm kinematics and functional properties in buffalo bulls. MATERIALS AND METHODS: Semen ejaculates (n = 90) collected during three seasons i.e. winter (n = 30), comfort (n = 30), summer (n = 30) were evaluated for sperm kinematics and functional properties. RESULTS: Sperm kinematics with respect to total (TM), progressive (PM) and rapid motility (RM) was higher (P<0.05) in fresh sperm compared to sperm that had been frozen-thawed. Similarly, all kinematic parameters [viz. average path velocity (VAP), straight linear velocity (VSL), curvilinear velocity (VCL), beats cross frequency (BCF), lateral head displacement (ALH), linearity (LIN) and straightness (STR)] were higher (P<0.01) at the fresh stage. With respect to season, frozen-thawed semen TM (57.67 ± 115%), PM (50.2 ± 1.15%) and RM (51.6 ± 1.19%) were higher (P<0.01) when using sperm collected during winter. The stage of cryopreservation (i.e., equilibration and freeze-thawing) also showed significant effects (P<0.01) on mitochondrial superoxide positive status (MSPS), mitochondrial membrane potential (MMP), acrosome status and intra-cellular calcium status. CONCLUSION: The season of sperm collection and cryopreservation have significant effects on buffalo bull sperm kinematics and functional properties.

Effects of Different Seasons on Milk Quality: A Study on Two Cattle Breeds in Rainy and Drought Contexts

The primary focus of dairy farming in the central region of Peru centers on producers. However, there is limited information on how different altitudinal zones, particularly during rainy and dry seasons, affect milk production. The present study aimed to investigate the effects of the rainy and dry seasons on the physicochemical properties of milk from Brown Swiss and Holstein cattle. A total of sixty cows were selected for the study, comprising 30 Brown Swiss and 30 Holstein. The study was conducted across two distinct seasons, including rainy and dry seasons. All animals received the same semi-intensive management and were fed ryegrass and balanced feed. Each animal provided 500 ml of milk for analysis in the morning. The milk was transported at a temperature of 2 °C, where they were analyzed with the Lactoscan equipment to evaluate protein, lactose, fat, total solids, milk density, freezing point, salts, and pH. Significant differences were observed in the interaction between Brown Swiss and Holstein breeds across different seasons, including rainy and dry periods. Significant differences were observed in protein content, showing a positive effect in the interaction “rain: Brown” a value of 3.50 ± 0.36, while “rain: Holstein” showed 3.14 ± 0.05. Statistical differences were observed in the interactions for lactose content, with rain: Brown at 4.82% and dry: Holstein at 4.37%. Similarly, there were significant differences in fat content and total solids for rain interaction of rain: Holsten, and dry: Brown. Nevertheless, no differences were observed in terms of milk density, freezing point, salts, and pH. It is concluded that there was an influence of the interaction between breed physiology and seasonal conditions on milk composition. The results also highlight the impact of season-specific environmental factors on the quality of milk.

Deciphering the seasonal dynamics of microplastic morphotypes and associated co-contaminants along the northwest coast of India

In the present study, the northwest coast of India, bordering the Arabian Sea, was selected to evaluate the microplastic (MP) abundance. This is the first study to emphasize the effects of different seasons on MP distribution. The collected MPs were dried, segregated, and evaluated based on their morphotype, size, color, and polymer type. A total of 1756.6, 7326.6, and 202 particles/kg of sand were estimated in the pre-monsoon, monsoon and post-monsoon seasons, respectively, with a dominance of polypropylene (PP) type of plastic in the pre-monsoon and high-density polyethylene (HDPE) in monsoon and post-monsoon seasons. HDPE and PP collected MPs during the monsoon season were further characterized for associated contaminants. Metal absorbance was detected using SEM-EDX mapping and ICP-MS. The presence of organic compounds (OCs) was analyzed using GC-MS. MPs exhibit distinct associations with metals, among which the HDPE pellet morphotype exhibits a higher range of metal adsorption. Total 61 different OCs were associated with MPs. The HDPE pellets contained the highest amounts of hydrophobic organic compounds. PP pellets were found to contain triglycerides, fatty aldehydes, and alkaloids, along with HOCs. Among morphotypes, pellet forms of MPs were found to adsorb more contaminants. These co-contaminants infiltrate the study area through sewage runoff and shoreline debris deposition, subsequently interacting with MPs. Furthermore, the MP diversity was studied by employing the MP diversity integrated index, which suggests that most of the MP diversity was observed in the pre-monsoon period. The pollution load index employed an MP risk assessment, which presented a low degree of MP contamination. In contrast, the polymer hazard index was calculated as 21650.3 in post-monsoon, placing the area under the extreme danger category. It is evident from the data that the types of MP is more important than their number. Thus, MP morphotypes have importance in the adsorption of co-contaminants.

Sizing Optimization of a Photovoltaic Hybrid Energy Storage System Based on Long Time-Series Simulation Considering Battery Life

An energy storage system works in sync with a photovoltaic system to effectively alleviate the intermittency in the photovoltaic output. Owing to its high power density and long life, supercapacitors make the battery–supercapacitor hybrid energy storage system (HESS) a good solution. This study considers the particularity of annual illumination due to climate conditions in Harbin, China. A global optimal PV-HESS sizing method is proposed by constructing a PV-HESS operating cost model and taking the annual system operating cost as the objective function. To consider the effect of battery life degradation due to different charge and discharge rates and charge and discharge times, a semi-empirical model based on the Arrhenius model was used to quantify the battery life degradation. Based on the effects of different seasons and different photovoltaic panel sizes, batteries, and supercapacitors on the optimization results, four scenarios are proposed. The feasibility of the system configuration corresponding to the four scenarios is discussed, and an optimal sizing configuration of the system is obtained. The simulation results show that the proposed method can effectively balance the degradation of the ESS due to irregular charging and discharging and determine the minimum operating cost and a reasonable sizing configuration of the system.

Detection of the dominant pathogens in diarrheal calves of Ningxia, China in 2021-2022.

Calf diarrhea is a complex disease that has long been an unsolved problem in the cattle industry. Ningxia is at the forefront of China in the scale of cattle breeding, and calf diarrhea gravely restricts the development of Ningxia's cattle industry. From July 2021 to May 2022, we collected diarrhea stool samples from calves aged 1-103 days from 23 farms in five cities in Ningxia, and performed PCR using specific primers for 15 major reported pathogens of calf diarrhea, including bacteria, viruses, and parasites. The effect of different seasons on the occurrence of diarrhea in calves was explored, the respective epidemic pathogens in different seasons were screened, and more detailed epidemiological investigations were carried out in Yinchuan and Wuzhong. In addition, we analyzed the relationship between different ages, river distributions and pathogen prevalence. Eventually, 10 pathogens were detected, of which 9 pathogens were pathogenic and 1 pathogen was non-pathogenic. The pathogens with the highest detection rate were Cryptosporidium (50.46%), Bovine rotavirus (BRV) (23.18%), Escherichia coli (E. coli) K99 (20.00%), and Bovine coronavirus (BCoV) (11.82%). The remaining pathogens such as Coccidia (6.90%), Bovine Astrovirus (BoAstV) (5.46%), Bovine Torovirus (BToV) (4.09%), and Bovine Kobuvirus (BKoV) (3.18%) primarily existed in the form of mixed infection. The analysis showed that different cities in Ningxia have different pathogens responsible for diarrhea, with Cryptosporidium and BRV being the most important pathogens responsible for diarrhea in calves in all cities. Control measures against those pathogens should be enforced to effectively prevent diarrhea in calves in China.

The effect of different seasons on the seminiferous tubules and leydig cells of the testis for the adult rabbit of the local breed (Histological study)

The season is an important factor determine the efficacy of the sexual activity of the adult males of different animal species, in this study, Sixteen adult male rabbit of local breed were used to investigate the effect of the different seasons of the year on the histology of the testicular tissue, particularly the leydig cells. Four animals were killed in each season and segments of testes processed by using histological technique. The results were indicated that the seasons winter and summer affect the seminiferous tubules reflected by subpopulation of different germ cells such as spermatogonia, primary and secondary spermatocytes, spermatids and spermatozoa. Leydig cells were atrophied with deeply basophilic nuclei associated with poorly blood capillaries in between, otherwise the seasons spring and autumn effect on the same germ cells and leydig cells, but in this case there was increase in the population of germ cells in the seminiferous tubules and the leydig cells were hypertrophied with the presence of cytoplasmic fat droplets and its nuclei were euochromatic, The blood capillaries in between these cells were abundant, these events indicated the increase activity of testicular tissue in both seasons, the (spring and autumn), While in winter and summer, this phenomena was contra versa. The present study was indicated that the spring and autumn seasons are the best for the progressed activity of the leydig cells and germ cells of the testicular tissue of the adult rabbit than the winter and summer seasons.

Effect of Different Seasons on the Milk Quality Parameters of Mohali District

The purpose of this research is to explore the effect of variation of different seasons on the composition of raw milk. The samples of raw milk were taken during winter, spring and summer season from a different region of Chandigarh. Biophysical and biochemical properties i.e. fat, protein, reducing sugars and lactose were analyzed. The result showed that summer milk had notable higher temperature, high pH, high reducing sugars, and high conductivity as compared to milk collected during spring and winter season. Whereas, protein, fat and lactose content were higher in winter as compared to spring and summer (P<0.05). Contaminated milk is a global cover and social issue that exists in the rural and municipal countries. Growing and developing areas are at higher risk due to a lack of monitoring and policies. Consumption of contaminated milk leads to various serious threats to health causes fatal diseases and could impact on the food industry. In various industries to check the milk adulteration requires expensive techniques which small vendors cannot afford. A comphersenive study has been carried out on the detection of milk adulteration kits.

Effects of different seasons on bacterial community structure in rose rhizosphere soil.

TO explore the changes of rhizosphere soil bacterial community of Rosa rugosa "Fenghua", Rosa rugosa cv. Plena and Rosa rugosa "Zizhi" in different seasons, the Illumina Miseq sequencing and the correlation network analysis of dominant flora was used. The results showed that the bacterial communities were mainly composed of Proteobacteria, Acidobacteria, Bacteroidetes, and Actinobacteria, with Sphingomonas, GP6, GP4, Novosphingobium, Wps-1_genera_incertae_sedis, and Massilia as the dominant genera. The correlation network analysis showed that, as the dominant group with the highest relative abundance, Sphingomonas had a significant positive correlation with Gemmatimonas, Aridibacter, GP3, GP4, and Flavisolibacter, and a significant negative correlation with Solirubrobacter, indicating that it could work synergistically with a variety of microorganisms to contribute to soil metabolism and the growth and development of roses. The results revealed the diversity of microbial structures in the rhizosphere soil of Rosa rugosa "Fenghua", Rosa rugosa cv. Plena and Rosa rugosa "Zizhi", and this will provide a theoretical basis for exploring the change rules of microbial communities, screening and utilizing beneficial microorganisms, and maintaining the growth and development of roses. KEY POINTS: • Variations from season to season significantly affected the bacterial community structure. • There was less variability in the bacterial community structure between rose varieties. • Sphingomonas was the dominant bacterium in all seasons.

Hydration Status in Men Working in Different Thermal Environments: A Pilot Study.

The aim of this study was to determine the effects of different seasons of the year and the time of day (before work vs. after work) on hydration status in men. The study involved sixty foresters who spent most of the work outdoors. During three seasons of the year (summer, autumn, and winter), indices of hydration status (body mass (BM) and percentage change of BM, total body water (TBW) and percentage change of TBW, serum osmolality (Sosm) and percentage change of Sosm, urine osmolality, urine-specific gravity (USG), urine color, and thirst) were determined before work on the first day (time point 1 used as baseline), immediately after work on the first day (time point 2), and before work on the following day (time point 3). USG decreased at time point 2 compared to time point 1 (p < 0.001) and time point 3 (p = 0.03). At time point 2 (p = 0.002) in winter and time point 3 in autumn (p = 0.049), serum osmolality was higher than in summer. In conclusion, the differences in hydration status depended on the time of day and season. A large percentage of foresters come to work inadequately hydrated, especially in colder seasons compared to summer.

Effect of season, lactation, parity and milk production on milk inflammatory parameters in healthy and mastitis infected Sahiwal cows

The present investigation was undertaken to study the effect of different season, stages of lactation, parity and milk production on milk inflammatory parameters in healthy and mastitis infected Sahiwal cows. Three parameters, viz. pH, electrical conductivity (EC) and somatic cell count (SCC) were taken to assess the quality of milk. The study was conducted on 100 selected Sahiwal cows maintained at livestock farm at National Dairy Research Institute, Karnal, Haryana. Significant higher values of pH, EC and SCC were found in milk samples during rainy season, followed by winter and summer. Effect of parity, lactation and milk production on inflammatory parameters was non-significant. The pH, EC and SCC also differed significantly in healthy, subclinical and clinical stages of mastitis. A significant positive coefficient of correlation was observed between SCC and EC (r=0.679) whereas moderate (r=0.526) but significant positive correlation was found in pH and EC. In view of above results, it can be deduced that the elevated value of pH, EC and SCC indicates inflammation into the udder of the animals which leads to reduced quality of milk. Hence it is imperative that pH, EC and SCC need to be considered along with effective management strategies.

The effect of different seasons on the rate of sperm DNA fragmentation in men

The effect of different seasons on the rate of sperm DNA fragmentation in men

Comparative analysis of the seasonal influence on polyphenolic content, antioxidant capacity, identification of bioactive constituents and hepatoprotective biomarkers by in silico docking analysis in Premna integrifolia L.

The present study reports the effect of different seasons on polyphenol content and antioxidant potential of ethanolic, methanolic, ethyl acetate, and aqueous extracts of leaves, stems, and roots of Premna integrifolia. Ethyl acetate extract of leaves (EAEPI) collected in the rainy season showed potent antioxidant activity with highest total phenol (74.33 ± 2.26µg/mg, gallic acid equivalent), and flavonoid (98.83 ± 0.26µg/mg, rutin equivalent) content. Therefore, EAEPI extract was subjected to characterization by UHPLC-Q-TOF-MS/MS and GC-MS analysis for the identification of active constituents. UHPLC-Q-TOF-MS/MS analysis in + ve ion mode revealed the presence of eight polyphenolic compounds namely quercetin-3-D-xyloside, kaempferol-3,7-O-bis-alpha-L-rhamnoside, isorhamnetin-3-Oglucoside, luteolin-3',7-di-O-glucoside, eriodictyol-7-O-glucoside, syringetin-3-O-galactoside, petunidin-3-O-beta-glucopyranoside and vitexin-2″-O-rhamnoside. GC-MS analysis confirmed the presence of 26 compounds with six major compounds viz; citronellol, phytol acetate, campesterol, squalene, stigmasterol, and hexadecanoic acid. These compounds are reported for the first time from P. integrifolia except phytol and stigmasterol. Our previous study validates the hepatoprotective potential of P. integrifolia but there was no idea about the bioactive compound responsible for the activity. So, in present work, the major compounds identified in spectrometry analysis were subjected to in silico docking against an important liver enzyme alanine amino transaminase to confirm its hepatoprotective properties. Docking analysis validates the presence of two hepatoprotective lead compounds stigmasterol, and campesterol, which satisfy the drug-likeness criteria with good absorption, distribution, metabolism, and toxicity properties. Thus, present work gives a clear insight about the influence of season on the total polyphenolic constituent in different plant parts of P. integrifolia, their antioxidant potential and preclinical evaluation of hepatoprotective lead compounds.

Impact of Ventilation Strategy on the Transmission of Outdoor Pollutants into Indoor Environment Using CFD

The transition to remote working due to the pandemic has accentuated the importance of clean indoor air, as people spend a significant portion of their time indoors. Amongst the various determinants of indoor air quality, outdoor pollution is a significant source. While conventional studies have certainly helped to quantify the long-term personal exposure to pollutants and assess their health impact, they have not paid special attention to the mechanism of transmission of pollutants between the two environments. Nevertheless, the quantification of infiltration is essential to determine the contribution of ambient pollutants in indoor air quality and its determinants. This study evaluates the transmission of outdoor pollutants into the indoor environment using 3D computational fluid dynamics modelling with a pollution dispersion model. Naturally ventilated buildings next to an urban canyon were modelled and simulated using Ansys Fluent and validated against wind tunnel results from the Concentration Data of Street Canyons database. The model consisted of two buildings of three storeys each, located on either side of a road. Two line-source pollutants were placed in the street, representing traffic emissions. Three internal rooms were selected and modelled on each floor and implemented with various ventilation strategies. Results indicate that for a canyon with an aspect ratio of 1, indoor spaces in upstream buildings are usually less polluted than downstream ones. Although within the canyon, pollution is 2–3 times higher near the upstream building. Cross ventilation can minimise or prevent infiltration of road-side pollutants into indoor spaces, while also assisting in the dispersion of ambient pollutants. The critical configuration, in terms of air quality, is single-sided ventilation from the canyon. This significantly increases indoor pollutant concentration regardless of the building location. The study reveals that multiple factors determine the indoor–outdoor links, and thorough indexing and understanding of the processes can help designers and urban planners in regulating urban configuration and geometries for improved indoor air quality. Future works should look at investigating the influence of indoor emissions and the effects of different seasons.

Standardization of beejakarishta preparation with respect to seasonal changes and the determination of its antibacterial activity against selected clinical isolates

Beejakarishta is an ‘arishta’ (self-generated alcoholic) preparation in Ayurveda with a wide range of pharmacological applications including in digestive disorders, anaemia, piles, inflammation, urinary tract infections (UTIs), diabetes, jaundice, etc. Even though, there are many studies which show the effect of different containers and method of preparation in the fermentation process of different arishta-asavas (fermented ayurvedic potion); no studies have been conducted to analyse the effect of different seasons and different time duration on the physicochemical characteristics and pharmacological potentials of beejakarishta. So, present study investigated the effect of season and duration in fermentation process of beejakarishta and variation in their analytical properties. In this study, six samples are made in April (summer season–greeshmaritu), October (autumn season - sarataritu) and in January (winter season -sisiraritu). In each season, three samples each were kept for 10 days fermentation and for 20 days fermentation. Fermentation tests and physicochemical analysis were carried out for all the samples and compared. HPTLC analysis was performed to evaluate the compounds present in the test samples. Beejakarishta with 20 days fermentation in summer season (BAG20), which showed typical physicochemical properties is examined for antibacterial analysis. 100 ?l of BAG20 showed significant antibacterial activity against Escherichia coli (18 mm), Staphylococcus aureus (19 mm) and Klebsiella pneumonia (26 mm). From the study, it was confirmed, seasons have a greater impact in determining the properties of the beejakarishta. The BAG20 can be developed as an effective novel antibacterial drugs for treatment of multidrug resistant (MDR) infection.

Assessing time dependent changes in microbial composition of biological crime scene traces using microbial RNA markers

Current body fluid identification methods do not reveal any information about the time since deposition (TsD) of biological traces, even though determining the age of traces could be crucial for the investigative process. To determine the utility of microbial RNA markers for TsD estimation, we examined RNA sequencing data from five forensically relevant body fluids (blood, menstrual blood, saliva, semen, and vaginal secretion) over seven time points, ranging from fresh to 1.5 years. One set of samples was stored indoors while another was exposed to outdoor conditions. In outdoor samples, we observed a consistent compositional shift, occurring after 4 weeks: this shift was characterized by an overall increase in non-human eukaryotic RNA and an overall decrease in prokaryotic RNA. In depth analyses showed a high fraction of tree, grass and fungal signatures, which are characteristic for the environment the samples were exposed to. When examining the prokaryotic fraction in more detail, three bacterial phyla were found to exhibit the largest changes in abundance, namely Actinobacteria, Proteobacteria and Firmicutes. More detailed analyses at the order level were done using a Lasso regression analysis to find a predictive subset of bacterial taxa. We found 26 bacterial orders to be indicative of sample age. Indoor samples did not reveal such a clear compositional change at the domain level: eukaryotic and prokaryotic abundance remained relatively stable across the assessed time period. Nonetheless, a Lasso regression analysis identified 32 bacterial orders exhibiting clear changes over time, enabling the prediction of TsD. For both indoor and outdoor samples, a larger number (around 60%) of the bacterial orders identified as indicative of TsD are part of the Actinobacteria, Proteobacteria and Firmicutes. In summary, we found that the observed changes across time are not primarily due to changes associated with body fluid specific bacteria but mostly due to accumulation of bacteria from the environment. Orders of these environmental bacteria could be evaluated for TsD prediction, considering the location and environment of the crime scene. However, further studies are needed to verify these findings, determine the applicability across samples, replicates, donors, and other variables, and also to further assess the effect of different seasons and locations on the samples.

Temporal and spatial dynamics of gastrointestinal parasite infection in Père David's deer.

BackgroundThe Père David’s deer (Elaphurus davidianus) population was established from only a small number of individuals. Their genetic diversity is therefore relatively low and transmissible (parasitic) diseases affecting them merit further attention. Parasitic infections can affect the health, survival, and population development of the host. However, few reports have been published on the gastrointestinal parasites of Père David’s deer. The aims of this study were: (1) to identify the intestinal parasites groups in Père David’s deer; (2) to determine their prevalence and burden and clarify the effects of different seasons and regions on various indicators of Père David’s deer intestinal parasites; (3) to evaluate the effects of the Père David’s deer reproductive period on these parasites; (4) to reveal the regularity of the parasites in space and time.MethodsIn total, 1,345 Père David’s deer faecal samples from four regions during four seasons were tested using the flotation (saturated sodium nitrate solution) to identify parasites of different genus or group, and the McMaster technique to count the number of eggs or oocysts.ResultsFour groups of gastrointestinal parasites were found, of which strongyles were dominant; their prevalence and burden were significantly higher than other groups. Significant temporal and spatial effects on gastrointestinal parasitic infection were found. Parasite diversity, prevalence, parasite burden, and aggregation were the highest in summer. Among the four regions, parasite diversity, prevalence, and burden were the highest in the Dongting Lake area. In addition, parasite diversity and burden during the reproductive period of Père David’s deer was significantly higher than during the post-reproductive period.ConclusionsThe summer season and the reproductive period of Père David’s deer had great potential for parasite transmission, and there is a high risk of parasite outbreaks in the Dongting Lake area.

Analysis of the impact of various charging modes on grid connection of electric vehicles

In recent years, as a new type of green energy, electric vehicles have developed rapidly. However, the charging behavior of electric vehicles is uncertain in time and season. A large number of electric vehicles connect the load with the power grid through different charging methods, which leads to the load redistribution of the power grid. Therefore, the research on the impact of load on distribution network under different conditions can give full play to the role of electric vehicles in peak load regulation and valley filling of power grid, which is of great significance to adjust charging behavior and improve power supply reliability of power grid.Firstly, according to the factors that affect the charging load of electric vehicles, the charger models under different charging modes and IEEE-14 node distribution network models are established. In addition, considering the daily living habits of the citizens, this paper proposes two normal distribution models to study the load distribution law of electric vehicles connected with the distribution network in different periods, and draw a one-day load curve. The peak load caused by people’s charging behavior in one day is explained and analyzed. Finally, the effects of different seasons on charging behavior and charging efficiency are simulated, and the load curves of different seasons are drawn, and the results are analyzed comprehensively.

EFFECT OF DIFFERENT SEASONS ON GROWTH PERFORMANCE, IMMUNE RESPONSES AND ANTIOXIDANT STATUS OF BROILER CHICKENS

The current experiment was carried out in farm nearby Fayoum research station to study effect of different seasons on growth performance of broiler chicks and detect early indicators for supplementing broilers flocks with antioxidant and immune enhancer. Four flocks from Arbor Acres broiler chicks were reared during summer, autumn, winter, and spring seasons in open side system. Productive performance parameters, complete blood count, antibody titers against Newcastle (NDV), antibody titers against avian influenza (H5N1) and some antioxidant parameters were measured. The following results were obtained: Body weight and body weight gain recorded significantly the highest values during spring seasons but the highest (P≤0.05) feed intake value was recorded during winter seasons and the best feed conversion value was recorded during autumn (P≤0.05). Total leukocytes count, heterophilus (H), lymphocyte (L) and H/L ratio were not affected by different seasons. Antibody titers against NDV and H5N1 record the highest significant values during summer season while the worst significant antioxidant status recorded during summer season. In contrast during winter values of antibody titers against NDV and H5N1 were significantly the worst but antioxidant parameters recorded significantly the best values. The results of the current study suggested that during winter flocks may need double doses from NDV and H5N1vaccines. However, during summer and autumn seasons, increasing antioxidant supplementation in diets or even drinking water is recommended.