The continuous use of crop residue in soil can enhance the crop yield and potential availability and mobility of P fractions to crops in conservation tillage. A field experiment was conducted to assess the long-term effects of surface crop residue applications on soil P fractions and their relationship with P uptake and crop yields. Field experiment was conducted at ICAR-Central Research Institute for Dryland Agriculture, Hyderabad, India during 2005 to 2022. The experimental treatments applied were: T1 - no crop residue application, T2 - application of crop residue @ 2 t ha-1, T3 - application of crop residue @ 4 t ha-1, and T4 - application of crop residue @ 6 t ha-1 in conservation tillage. The test crops viz. sorghum (Sorghum vulgare) and cowpea (Vigna unguiculata) were grown in a yearly rotation every year in the kharif season in conservation tillage. The results of the long-term study revealed that the per cent increase in sorghum (Sorghum vulgare) grain yield with the application of crop residue at the rate of 2, 4 and 6 t crop residue ha-1was to the tune of 7.9, 16.1 and 26.0%, respectively over no crop residue application. Similarly, the per cent increase in the cowpea (Vigna unguiculata) seed yield was to the extent of 25.2, 43.0 and 64.1% in 2, 4 and 6 t ha-1, respectively over no crop residue application. The sorghum and cowpea stover yield also significantly (p=0.05%) increased with the added levels of the crop residue. Significantly higher P uptake by sorghum and cowpea was recorded in 6 t crop residue ha-1 as compared to the other crop residue treatments. Results of the current study also showed the significant positive response of long-term crop residue application on the P fractions viz., water-soluble phosphorus (WS-P), aluminum bound P (Al-P), iron bound P (Fe-P), calcium bound P (Ca-P) and reductant soluble P (RS-P), and on available P and total P in soil as compared to no crop residue application. The average percent contribution of P fractions toward the sum-total of extracted P was in the order: Fe-P (30.8%)>RS-P (30.7%)>Al-P (24.2%)>Ca-P (9.4%)>WS-P (4.8%). The WS-P and Al-P were found to be the P fractions indicating their specificity by cowpea crop, and WS-P and Ca-P were found to be P fractions indicating their specificity by sorghum crop. The results of the present study will be highly useful in understanding the effect of crop residue application in conservation tillage on the P fractions and their contribution toward P uptake and crop yields.