Effectiveness Of Beta-tricalcium Phosphate Research Articles

EFFECTIVENESS OF PLATELET- RICH FIBRIN WITH BETA TRI-CALCIUM PHOSPHATE PLUS CALCIUM SULFATE ON PERIODONTAL INTRABONY DEFECT MANAGEMENT (RANDOMIZED CONTROLLED CLINICAL STUDY)

The purpose of this study is to evaluate the effectiveness of beta tri-calcium phosphate plus calcium sulfate mix, with or without platelet rich fibrin in reducing probing periodontal pockets clinically and defect depths radiographically, while increasing clinical attachment levels to reconstruct intrabony defects in human clinical trials. Materials and Methods: Sixteen participants with periodontal intrabony defects ≥ 5 mm were divided into two equal groups. The first eight intrabony defects in study group received platelet rich fibrin mixed with EthOss ®; while the eight remaining intrabony defects in control group were reconstructed only with EthOss® synthetic bone substitute. Freidman ANOVA and Mann-Whitney tests were chosen for intra-group and inter-group comparisons, respectively. Results: Final outcomes revealed statistically significant decrease in periodontal pocket depth and defect depth values (P ˂ 0.05) with a statistically significant clinical attachment level gain in study and control groups. Precisely, mean values of all measured categories were greater in study rather than control group participants, as confirmed clinically by William’s probe and radiographically by cone-beam computed tomography at 6 months after surgery. Conclusion: Adding PRF to β-TCP plus CS is highly effective in inducing new periodontal soft and hard tissue synthesis resulting in better clinical and radiographic outcomes when surgically incorporated in periodontal intrabony defect(s).

Histological Evaluation of Effect of beta-Tricalcium Phosphate on Bone healing in Alloxan-Induced diabetes

Background: Improved glucose level control with insulin injections have allowed for the diabetic population to live longer and healthier lives. Unfortunately diabetes remains a worldwide epidemic disease with multiple health implications. Specifically, its effects upon fracture healing are compromised in diabetics with as high as 87% recovery delay relative to “healthy” counterparts. Current medical treatments for bone injuries have been largely focused on replacing the lost bone with allogenic or autogenous bone grafts, beta-tricalcium phosphate (β -TCP), a ceramic alloplast, has interconnected system of micropores, has been widely used as a biologically safe osteoconductive bone substitute. The aim of this study was histological evaluation of effect of topical application of β –TCP on bone healing of diabetic rabbit. Materials and methods: Sixty New Zealand rabbits used in this study were divided into three groups for four healing intervals the experimental groups were: 1-Control group(C).2-Diabetic rabbits received insulin treatment regarded as controlled diabetes mellitus (CDM)group.3-Diabetic rabbits did not receive any treatment regarded as uncontrolled diabetes mellitus (UDM)group. All animals subjected to surgical operation in right tibia, creating bone defect 3mm in depth and 4mm in diameter filled with β-Tricalcium Phosphate. Animals' scarifications were done in 5 day, 2, 4 and 6 weeks durations. Routine processing and sectioning technique was performed for histological evaluation. Results: Histological findings indicated that bone defects in control(C) and controlled diabetes mellitus (CDM) groups showed early bone formation, mineralization and maturation in comparison to healing of uncontrolled diabetes mellitus (UDM) group. Histomorphometric analysis for all bone parameters examined in this study, showed variation in significance among all groups in different durations. Conclusion: The study revealed that application of β-TCP was more effective in enhancement of bone regeneration and in acceleration of bone healing process in controlled diabetes as compared to the uncontrolled one.

The effect of beta-tricalcium phosphate on mechanical and thermal performances of poly(lactic acid)

Orthophosphates are bioactive crystals with similar structure, in terms of elemental composition and crystal nature, to human bone. In this work, biocomposite materials were prepared with poly(lactic acid) (PLA) as matrix, and beta-tricalcium phosphate (β-TCP) as osteoconductive filler by extrusion-compounding followed by conventional injection molding. The β-TCP load content was varied in the 10–40 wt% range and the influence of the β-TCP load on mechanical performance of PLA/β-TCP composites was evaluated. Mechanical properties of composites were obtained by standardized tensile, flexural, impact, and hardness tests. Thermal analysis of composites was carried out by means of differential scanning calorimetry; degradation at high temperatures was studied by thermogravimetric analysis; and the effect of the β-TCP load on dynamical response of composites was studied by mechanical thermal analysis in torsion mode. The best-balanced properties were obtained for PLA composites containing 30 wt% β-TCP with a remarkable increase in the Young’s modulus. These materials offer interesting properties to be used as base materials for medical applications such as interference screws due to high stiffness and mechanical resistance.

Histological Evaluation of Effect of Beta-Tricalcium Phosphate on Bone Healing in Alloxan-Induced Diabetes

Histological Evaluation of Effect of Beta-Tricalcium Phosphate on Bone Healing in Alloxan-Induced Diabetes

Evaluation of horizontal ridge augmentation using beta tricalcium phosphate and demineralized bone matrix: A comparative study

Objectives: To evaluate the effectiveness of beta tricalcium phosphate (β-TCP) alone compared to β-TCP and Demineralized Bone Matrix (DBM) in regenerating localized horizontal maxillary alveolar ridge deficiencies prior to implant placement. Study Design: The study included 20 patients with horizontal maxillary ridge deficiencies limited to one or more neighbouring teeth and initial ridge width of ≤ 5mmm. Patients were divided equally into two equal groups. Ridge augmentation was performed using Guided Bone Regeneration (GBR) principals. In group I GBR was performed using β-TCP only, while in group II both β-TCP and DBM were used. Following a 6 months healing period, bone cores from both groups were retrieved and implants were inserted. Specimens were examined histologically to calculate percentage of mineralized bone. Apical and crestal changes in ridge dimensions were calculated by digital subtraction using Cone Beam Computed Tomography (CBCT) immediately after graft placement and six months later. Results: There was a statistically significant difference between the mean area percentage of mineralized bone between both groups where it was 40.1 % (range: 27.76-% 66.29 %) for group I and 68.96 % (range: 60.07 % - 87.33 %) for group II. Radiograpically, the mean ridge width in group I increased crestally to 4.66 mm (range:3.5-5mm) and apically to 6.12 mm (range: 4.1-6.7 mm). In group II the mean ridge width increased crestally to 5.2 mm (range 4.9-5.4mm) and apically to 6.9 mm (range 6.0-7.8 mm). Group II showed more bone gain with a mean of 1.37 mm crestally and 2.44 mm apically. This difference however was not statistically significant Conclusion: Within the limitations of this study the combination of DBM and β-TCP can be used effectively in cases exhibiting minimal alveolar ridge defects. Key words:Guided bone regeneration, equine bone, alloplast, bone graft.

Coverage of gingival recession defects using acellular dermal matrix allograft with or without beta-tricalcium phosphate

The aim of this study was to investigate the effect of beta-tricalcium phosphate (β-TCP) particles in combination with acellular dermal matrix (ADM) allograft in gingival recession. Experimental gingival recession defects were created in beagle dogs and randomly assigned to one of the following groups: ADM, ADM + β-TCP, or coronally positioned flap (CPF; control). Tissues were histologically examined at 4, 8, or 16 weeks following treatment. A greater thickness of gingiva was observed at the sites treated in both the ADM + β-TCP and ADM groups than in the CPF group. The ADM + β-TCP group showed a statistically significant increase in both new bone and cementum formations compared to the ADM group. The results suggest that the combination of β-TCP and ADM is more effective in promoting new bone and cementum formations than ADM graft alone.

Effect of an injectable 3D scaffold for osteoblast differentiation depends on bead size

The objective of this study was to evaluate the effect of beta-tricalcium phosphate (beta-TCP) bead size on the behavior of KUSA/A1 mouse osteoblasts when the beta-TCP beads are used as the solid phase of a scaffold in which alginate was used as the gel phase. KUSA/A1 cells were loaded onto a three-dimensional (3D) scaffold fabricated from beta-TCP beads with diameters ranging from 300 to 500 microm (small beads), 500-700 microm (medium beads) and 700-850 microm (large beads); cells were cultured for 3, 7 and 14 days. Scanning electron microscope observations showed that each bead was connected in a network consisting of the alginate gel and KUSA/A1 cellular matrix that was tightly bonded to form a 3D structure. After 3 days, cells in the 3D scaffold with medium beads had a significantly higher alkaline phosphatase activity (ALP) than cells in the other scaffolds. However, by 7 and 14 days in culture there was no significant difference in DNA levels, ALP activity or osteocalcin expression. At 8 weeks, only the composite containing small beads and KUSA/A1 cells had turned completely into bone in vivo. Thus, bead size may influence the success of bone formation in this context.