Effect Of Benzyladenine Research Articles

Effects of Benzyl Adenine and Thidiazuron on Shoot Regeneration of Cavendish Banana

Effects of Benzyl Adenine and Thidiazuron on Shoot Regeneration of Cavendish Banana

The Effects of Benzyladenine and Tomato (Solanum Lycopersicum L.) Extract Concentrations on Shoot Induction of Cattleya sp. Orchid Under In Vitro Culture

The Effects of Benzyladenine and Tomato (Solanum Lycopersicum L.) Extract Concentrations on Shoot Induction of Cattleya sp. Orchid Under In Vitro Culture

Effects of Benzyl adenine (BA) and Gibberellic acid (GA3) on physiological and biochemical attributes of gladiolus (Gladiolus grandiflorus L.) spikes

The uniform sized corms (3.5-4.0 cm dia) of Gladiolus grandiflorus L. were dipped in various concentrations of BA (50,75, 100 and 125 mg/l) and GA3 (50 , 75 , 100 and 125 mg/l) for 24 hrs. The Membrane stability index (MSI), Relative water content (RWC), total soluble sugars and total protein content of florets increased with the increased concentrations of BA and GA3. The post harvest quality parameters also improved with BA and GA3 treatments as compared to control but GA3 had more significant effect than BA. The treatment with 125 mg/l GA3 significantly hastened the opening of basal floret, increased the floret size, number of florets open at one time, per cent of opening of florets, water absorbed and led to maximum reduction in pH that ultimately led to improvement in vase life. Bangladesh J. Bot. 51(2): 297-304, 2022 (June)

Effects of Benzyladenine on in vitro ‘Hom Rangsi’ Rice and Induction of Aluminum Acid Tolerance Lines by Gamma Irradiation

‘Hom Rangsi’ was the non-photoperiod aromatic mutant rice which derived from fast neutron radiation KDML 105. ‘Hom Rangsi’ seeds were cultured on MS solid medium without any supplemented for a week. And then, all explants were placed on MS (Murashige and Skoog, 1962) medium supplemented with 0, 5, 10, 15, 20, 25 mg/L BA (benzyladenine) for multiple shoot induction. The optimal concentration of BA for induced multiple shoot induction of ‘Hom Rangsi’ line was MS + BA 25 mg/L, the highest number of shoots were 5.38 shoot/seed. The following experiment was done, irradiated ‘Hom Rangsi’ seeds with 0, 100, 200, 300, 400 Gy gamma ray which cultured on MS solid medium supplemented with 400 mg/L Al3+ pH 2.9 were selected for acid tolerance lines. After six weeks cultured, the survivals of irradiated plantlets were 86.32, 77.78, 58.95, 58.95, 21.87% and the height of irradiated plantlets were 8.4, 8.3, 6.7, 6.6, 6.1 cm respectively without any shoot budding. All survival plantlets were transferred to suitable MS + BA 25 mg/L medium which discovered from the first experiment for multiple shoot budding. After six weeks cultured, the maximum of 5.24 shoots/plantlet were found from 300 Gy irradiation significantly and followed by 400, 200, 0 and 100 Gy irradiation treatments which gave 4.55 and 4.41, 4.37 and 4.31 shoots/planlet respectively.

Physiological characteristics and vase life responses of rose cut flowers (Rosa hybrida L. cv. ‘Royal Baccara’) to benzyl adenine and 1- metylcylcopropene

+prolonging vase life and decreasing post-harvest losses are considered a basic strategy towards sustainable development of agriculture, and the application of growth regulators, e.g. benzyl adenine(BA) and 1-metylcylcopropane(1-MCP), is one of the practical methods for them. The effects of benzyl adenine(BA) and 1-metylcylcopropene(1-MCP) on some biochemical characteristics and vase life of rose cv. ‘Royal Baccara’ (Rosa hybrida L. cv. ‘Royal Baccara’), were explored in a factorial experiment based on a randomized complete block design with three replications in 2017. The factors included BA at three levels of 0 (control), 75 and 150 ppm and 1-MCP in three levels of 0 (control), 1 and 2 µl/L-1. The results showed that the maximum soluble protein content was observed in 150 (ppm) BA+ 2 (µl.L-1) 1-MCP treatments on the second, fourth, sixth, and eighth days. The results of this research revealed that BA at the rates of 75 and 150 ppm and 1-MCP at the rates of 1 and 2 (µl.L-1) enhanced soluble protein content by 10.49, 13.37, 15.18, and 18.18 percent as compared to the control, respectively. The results showed that the maximum proline content was related to the control and the minimum content was observed in the plants treated with 150 (ppm) BA+ 2 (µl.L-1) 1-MCP. The endoproteases enzyme content was slightly decreased on the eighth day, indicating the efficacy of BA and 1-MCP in inhibiting the senescence process and prolonging vase life of rose flowers. The results showed that the application of 1 or 2 μl.L-1 1-MCP and 150 mg L-1 BA can improve the vase life of cut roses

Effect of Benzyl Adenine, Indole Acetic Acid and Gibberellic Acid on Vegetative Growth, Chemical Constituents and Volatile Oil Attributes of Sweet Basil Plants

Benzyl adenine, indole acetic acid and gibberellic acid at different concentrations were sprayed to study their effects on growth, chemical components and volatile oils of sweet basil in 2018 and 2019 seasonsPlant height, number of leaves/plant, average leaf area, and herb fresh and dry weights/plant greatly improved with the applied treatments especially with IAA treatments followed by GA3 treatments. However, the number of lateral branches/plant and stem diameter were increased with BA treatments especially with 10 ppm than 5 ppm. Generally, the second cut was superior in the studied vegetative growth parameters than the first cut. The highest values of N, P, K, Fe, and Mn were recorded with BA at 10 ppm, whereas higher Zn values were recorded with BA at 5 ppm. Active ingredients including total phenols, total flavones, total chlorophyll, and L- ascorbic acid greatly increased with IAA at 50 or 100 than the other treatments or the control. However, volatile oil percentage and volatile oil content/plant were high in IAA at 50 or 100 ppm followed by GA3 at 200 ppm. The second cut was superior to the first cut in promoting volatile oil percentage and volatile oil content/plant in both studied seasons. The main components in volatile oil were 1,8-cineole, linalool, a-trans-bergamotene, germacrene D and a-muurolol. The highest values of linalool, the main component in the volatile oil of sweet basil (50.90%) was recorded by IAA at 100 ppm.

The Effect of Benzyl Adenine on Qualitative Characteristics and Antioxidant Capacity of Plum Fruit during Storage

The Effect of Benzyl Adenine on Qualitative Characteristics and Antioxidant Capacity of Plum Fruit during Storage

Growth and Flower Bud Induction in Strawberry ‘Sulhyang’ Runner Plant as Affected by Exogenous Application of Benzyladenine, Gibberellic Acid, and Salicylic Acid

Strawberry (Fragaria × ananassa) is one of the most important and popular fruit crops in the world, and ‘Sulhyang’ is one of the principal cultivars cultivated in the Republic of Korea for the domestic market. The growth and flower induction in strawberry is the process which influences directly on fruit bearing and yield of this crop. In this study, effect of benzyladenine (BA), gibberellic acid (GA3), and salicylic acid (SA) on growth and flower bud induction in strawberry ‘Sulhyang’ was investigated. The 3-week-old runner plants, grown in 21-cell propagation trays, were potted and cultivated in growth chambers with 25oC/15oC (day/night) temperatures, 70% relative humidity (RH), and light intensity of 300 μmol·m-2·s-1 photosynthetic photon flux density (PPFD) provided by white light emitting diodes (LEDs). The runner plants were treated with one of three concentrations, 0 (control), 100, and 200 mg·L-1 of BA, GA3, or SA solution. The chemicals were sprayed two times on leaves of runner plants at an interval of two weeks. After 9 weeks the results showed that the application of all chemicals caused reduction of root length and chlorophyll (SPAD) content as compared to the control. The lowest chlorophyll (SPAD) content was recorded in plants treated with GA3. However, the treatment of 200 mg·L-1 GA3 promoted leaf area, leaf fresh weight, and plant fresh weight. The greatest flower induction (85%) and number of inflorescences (4.3 inflorescences per plant) were observed in the treatment of 200 mg·L-1 SA, followed by 100 mg·L-1 SA. Overall, results suggest that foliar application of GA3 solution could accelerate plant growth, while foliar application of SA solution could induce hastened flowering. Further studies may be needed to find out the relationship between GA3 and SA solutions treated in a combination, and the molecular mechanism involved in those responses observed.

EFFECT OF BENZYLADENINE ON MICROPROPAGATION OF BANANA SHOOTS TIP

The effect of benzyl adenine (BA) at 0, 2, 4, 6 and 8 mg l-1 on micropropagation of banana shoot tips was studied. This study also included the morphological responses of banana shoot tips especially with 0 & 6 mg l-1 of BA treatments in relation to some biochemical compositions (total soluble phenols, free amino acids, total soluble sugars, chlorophyll a, b and carotenoids). Growth in 6 mg l-1 of BA resulted in increase in the most morphological parameters compared to the rest treatments. The results showed that 6 mg l-1 of BA treatment significantly increased fresh and dry weights, number of shootlets, shootlet and root length and number of leaves and roots/plantlet as compared without BA. Accumulation of total soluble sugars, free amino acids and chlorophylls was enhanced by 6 mg l-1 of BA while the reverse was true with the rest biochemical compositions (total soluble phenols and carotenoids). The biochemical status and BA treatment at 6 mg l-1 during micropropagation of shoot tips in banana may be important for the development and optimization of strategies for large scale propagation and germplasm conservation.

Effects of benzyladenine and α-naphthaleneacetic acid on the early growth phase of Ananas comosus (L.) Merr.

In this study, benzyladenine (BA) and α- naphthaleneacetic acid (NAA) are used to study the growth of pineapple fruit (Ananas comosus (L.) Merr.) in the early growth phase. Pineapple fruits are sprayed with BA or NAA at different concentrations (1, 5, 10, 20, 40 and 60 mg.L-1) at both 0 and 3 days after flowering (DAF, 50 % dried flowers). Fruits are sampled every 15 days from 0 to 75 days to survey to weight, size and qualities of pineaple fruit. Besides, fruits (which are sprayed with BA or NAA at concentration 20 mg.L-1) are sampled at 15 days for the cell size observation. The results showed that the treatments with three highest concentration of BA or NAA (20, 40 và 60 mg.L-1) significantly increased fruit weights and fruit sizes. Exogenous BA or NAA at the concentration 20 mg.L-1 increased the fruit weights by increasing the number of flesh cells without negative effects on fruit qualities.

Effects of benzyladenine and α-naphthaleneacetic acid on the early growth phase of Ananas comosus (L.) Merr.

In this study, benzyladenine (BA) and α- naphthaleneacetic acid (NAA) are used to study the growth of pineapple fruit (Ananas comosus (L.) Merr.) in the early growth phase. Pineapple fruits are sprayed with BA or NAA at different concentrations (1, 5, 10, 20, 40 and 60 mg.L-1) at both 0 and 3 days after flowering (DAF, 50 % dried flowers). Fruits are sampled every 15 days from 0 to 75 days to survey to weight, size and qualities of pineaple fruit. Besides, fruits (which are sprayed with BA or NAA at concentration 20 mg.L-1) are sampled at 15 days for the cell size observation. The results showed that the treatments with three highest concentration of BA or NAA (20, 40 và 60 mg.L-1) significantly increased fruit weights and fruit sizes. Exogenous BA or NAA at the concentration 20 mg.L-1 increased the fruit weights by increasing the number of flesh cells without negative effects on fruit qualities.

Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

The establishment and characterization of cell suspension cultures are an in vitro culture technique very useful for various plant biotechnological applications (production of secondary metabolites, mass micropropagation, protoplast isolation and fusion, gene transfer and the investigation of cell pathways). The objective of this study was to establish and characterization of cell suspension cultures of V. planifolia by inducing friable calluses. For that, friable calluses were obtained from immature seeds cultivated in MS medium supplemented with 0.45µM thidiazuron (TDZ). The effect of benzyladenine (BA) in different concentrations was evaluated. Cultures were incubated under photoperiod at continuous stirring at 120rpm on an orbital shaker. The optimal condition found for biomass growth in suspension cultures was 0.5g of inoculum density (fresh weight) in MS liquid, supplemented with 8.88µM BA. The growth kinetics of the cell suspension culture revealed a maximum cell growth (exponential growth phase) at day 16 and an 80% cell viability. The establishment and characterization of cell suspension cultures of V. planifolia constitute the bases of future studies and above all a better biotechnological use of this crop.

Pengaruh Jenis Pupuk dan Konsentrasi Benzyladenin (BA) Terhadap Pertumbuhan dan Pembungaan Anggrek Dendrobium Hibrida

Dendrobium as ornamental plants that are used as cut flowers, potted plants, wreaths, garden ornament has a high economic value (due to the type, beauty, shape, texture, flower size, and rarity), has a broad market opportunities, sources of germplasm as mains to cross in order to get new varieties, and to cultivate them do not require large tracts of land. to get orchid production which is reached with good cultivation, one of them is by using fertilizers and growth regulators (PGR) to obtain growth and good flowering of orchids. This study was conducted to answer the following questions: (1) Which type of foliar fertilizer that can generate the best response to the growth and flowering of orchid Dendrobium, (2) how the effect of benzyladenine (BA) on the growth and flowering of orchid Dendrobium, (3) whether there is an interaction between foliar fertilizers and provision of benzyladenine (BA) to influence the growth and flowering of orchid Dendrobium. The research conducted in the greenhouse, Faculty of Agriculture, University of Lampung from January to June 2014. The experiments were carried out with a complete randomized block design with three replications. The treatments arranged in factorial (2x5). The first factor of 2 types of fertilizers, was (NPK 20-15-15) and (NPK 10-40-15). The second factor is the various concentrations of BA are: 0, 100, 200, 300, and 400 mg/l. based on data and discussion of experimental results that have been raised, it can be concluded as follows: Gandasil fertilizer (NPK 20-15-15) and Hyponex (NPK 10-40-15), had no effect on the growth and flowering of plants Dendrobium hybrids indicated by a variable percentage of plants to germinate, the number of new shoots, the height of new shoots, the increase of leaf pseudo stem diameter, and the percentage of flowering plants. Application of benzyladenine (BA) on the concentration of 100-400 mg / l, can stimulate flowering orchid hybrid Dendrobium, which is indicated by an increase in the percentage of flowering from 60.50 to 64.83%. Application of BA on the concentration of 100-400 mg / l had no effect on the percentage of ornamental plants whis have new sprout, and the number and the height of new shoots. There is no interaction between the two types of NPK (20-15-15 and 10-40-15), with concentrations of BA in influencing all observed variables. Keywords: Dendrobium, Two kinds of completed fertelizers, Benzyladenine, flowering

Investigation on the effect of benzyladenine on the germination, radicle growth and meristematic cells of Nigella sativa L. and Allium cepa L.

The effect of different benzyladenine (BA) treatments which is a phyto-regulator or plant hormone on the cytology and growth of Nigella sativa L. (Black seed) and Allium cepa L. (onion) were investigated. Six concentrations of benzyladenine ranging from 5 to 55ppm were applied for 6, 12, 18, 24, 36 and 48h. The treatments elevate the germination percentages of Nigella sativa L. and Allium cepa L. and increase the root growth of both plants. In contrast concentrations higher than 60ppm for 48h were caused an inhibition effect for both plant. The root growth initiation was concentration and/or time dependent. The applied concentrations of BA showed a promotor effect on cell division in root tips of both plants and caused an increase in their mitotic index values (MI). The elevation in MI values in root tips of Nigella sativa L. was more evident than that of Allium cepa L. All treatments changed the frequency of mitotic phases as compared with the control values. All the applied concentrations of BA significantly induced a number of chromosomal aberrations in root tip cells ofNigella sativa L. and Allium cepa L. The total percentages of abnormalities in Nigella sativa L. root tip cells were more than that in Allium cepa L. with all concentrations of BA. The most dominant types of observed abnormalities were stickiness, bridges, and C-mitosis. BA treatments produced a number of mitotic abnormalities in dividing cells in root tips of both plants resulting from its action on the spindle apparatus such as C-mitosis, lagging chromosomes and multipolar at ana-telophases. Also, BA induced vacuolated nuclei and irregular prophases. The induction of chromosomal stickiness and chromosomal aberrations such as bridges indicates its action on the chromosome. Also, the induced chromosomal bridges at ana-telophases indicates true clastogenic potential of this chemical. It may be concluded that BA causes toxic effect on root tip cells of Nigella sativa L. and Allium cepa L. and this toxicity induces different types of genic and chromosomal variations.

Effects of benzyladenine and nitrogen on growth characteristics of pistachio seedlings, cv. Badami Zarand, under salinity stress

Effects of benzyladenine and nitrogen on growth characteristics of pistachio seedlings, cv. Badami Zarand, under salinity stress

Effect of benzyl adenine on the in vitro culture of young female flowers of Breadfruit (Artocarpus altilis (Park.) Fosberg)

Breadfruit (Artocarpus altilis (Park.) Fosberg) is a woody tropical tree. In addition to the nutritious value, breadfruit is also a precious medicinal source, because every part of it contains many natural compounds with biological activity. With the aim of providing a pharmaceutical source cond food with high yield, as well as the short harvert time, we examined the effects of the concentration of benzyl adenine (BA) on the in vitro culture of young female breadfruit flowers. The results showed that in the first phase of the culture, the concentration of 5 mg/L BA stimulated an increase in size of each female flowers, especially in the calyx. The increasing concentration of 10 mg/L BA stimulated the formation of callus in the calyx of each female flowers. Roles of respiration rate and endogenus hormones were discussed to understand the physiological changes in the in vitro culture of young female breadfruit flowers.

Effect of Terminal Water Stress and Benzyl Adenine on Osmoregulants in Pearl Millet [Pennisetum glaucum(L.) R. Br.] Genotypes

The effect of benzyl adenine (BA) soaked seed treatment and terminal water stress in leaf tissues of four genotypes of pearl millet (J-2340, 95444B, J-2290 and JMS-101B) under field conditions was studied during summer season. The leaves were collected at terminal water stress stage and used for proline, free amino acids, soluble sugars and phenol estimation. Imposition of terminal stress increased all these four parameters. Free amino acids (FAA) were accumulated in all the genotypes on imposing terminal stress condition. The overall mean for FAA content was higher in drought sensitive genotypes J-2290 and JMS-101B as compared to tolerant genotypes. Water soaked seed treatment showed higher free amino acids. Terminal stress condition increased proline content (2.2 fold) than the irrigated condition and it was accumulated in all the genotypes with higher accumulation in J-2340 and J-2290. Among the presoaking treatments, less proline accumulation was observed in water soaked and BA soaked treatments. On exposure to the terminal stress, all the genotypes showed rise in the total soluble sugars and the greater accumulation was observed in genotype JMS-101B, while the least was in the genotype 95444 B. The overall mean of total soluble sugars was significantly higher in the genotype JMS-101B and the lowest in the J-2340. The presoaking treatments did not show any significant effect. The phenol content was also increased under the terminal stress condition in all the genotypes and a significant higher accumulation was in the genotype J-2290. The overall mean values of phenol in genotypes were the highest in JMS-101B and minimum in the genotype 95444B. The presoaking treatments viewed the highest content in the BA soaked treatment and the lowest in the control.

Effect of benzyladenine (BA) on auxin-induced stem elongation and thickening in tulip (Tulipa gesneriana L.)

It is well known that stem elongation in tulip is induced by the auxin produced in the leaves and gynoecium. Excision of the flower bud and all the leaves in an early stage of tulip growth resulted in almost total inhibition of stem growth, but the inhibition was completely recovered by the exogenous application of auxin to the place from which the flower bud had been removed. Hormonal control of stem thickening in tulip is much less known. Additional application of benzyladenine (BA) to the tulip stem by soaking a cotton wick wrapped around all the internodes only slightly inhibited stem growth induced by IAA at a concentration of 0.1 and 2.0%, but substantially stimulated the thickening of all the internodes. The treatment of the tulip stem with benzyladenine enabled direct contact of the cytokinin with the epidermis, which is an important factor in stem elongation. The experiment conducted in field conditions also showed that BA only slightly inhibited the elongation of the fourth internode induced by IAA, but stimulated the thickening of that internode. IAA applied at a concentration of 2.0% stimulated ethylene production to a much higher extent than IAA at a concentration of 0.1%, and BA affected the auxin-induced ethylene production only to a small extent. Metabolic significance of these findings is discussed.

Effects of benzyl adenine and gibberellic acid pre-treatments on dormancy release, flowering time and multiplication of oriental lily (Lilium longiflorum) bulbs

Effects of benzyl adenine and gibberellic acid pre-treatments on dormancy release, flowering time and multiplication of oriental lily (Lilium longiflorum) bulbs

Dikegulac Sodium and Benzyladenine Effects on Six Landscape Tree Species during Container Production

A study was conducted to evaluate dikegulac sodium (dikegulac) and benzyladenine (BA) as branching agents on landscape trees during production. Common among the six species in this two-year study was an increase in new shoot development following the application of dikegulac. Relative to shoot counts of nontreated plants, trees treated with a single foliar application of 800 to 3200 ppm of dikegulac had an increase in shoot numbers of 29 to 107% in Japanese maple, 75 to 158% in red maple, 67% in redbud, 50 to 65% in bald cypress, and 56 to 103% in black gum. Nontreated plants of green ash formed only one or two lateral shoots in 2011, whereas dikegulac-treated green ash had 10 to 12 new shoots. In 2012, green ash treated with 200 to 800 ppm of dikegulac developed 100 to 150% more new shoots than nontreated green ash. Foliage of all species, except Japanese maple, was injured to varying degrees by dikegulac, but the injury dissipated over the growing season. BA promoted increased shoot development only in bald cypress, and canopies of that species were visually fuller and more compact than those of bald cypress treated with dikegulac.