Lately, there has been an increased use of e-portfolios in tertiary institutions, and this tool has implications for students studying practical courses. However, very few studies have examined pre-service teachers’ and nurses’ perceptions towards the use of e-portfolios as a pedagogical tool. The purpose of this study was to examine pre-service teachers’ and nurses’ perceptions towards the use of e-portfolios in their training. To achieve this aim, 300 pre-service teachers and nurses were selected from a Ghanaian University and surveyed. The findings show that the majority of the respondents believe that the use of e-portfolios has a positive impact on their professional training. The study also revealed significant differences between pre-service teachers’ and nurses’ perceptions about the use of e-portfolios. The study further shows that, although there are challenging factors with the use of e-portfolios, the majority of the respondents prefer the use of e-portfolios as a teaching, learning, and assessment tool.
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