Edible Roots Research Articles

Study of the importance of plants with tubers and edible roots in the life of local populations in the Nimba Mountains Biosphere Reserve

This study focusing on root and tuber plants in the riverside villages of Nimba aims to promote the importance of roots and tubers in the lives of people living near the UNESCO heritage site, Nimba Mountains Biosphere Reserve. . The method of collecting data from resource people and the focus group through a questionnaire developed using the Kobocollect tool was used. The survey concerned 190 people including 79 women or 41.58% who were grouped into age groups with an interval of 5 years and the age group over 20 years was the most interested with 143 people or 75 .26% of respondents. Among all agricultural productions, cassava ranks first (42%), followed by taro (30%), sweet potato (18%) and yam (10%) the least cultivated due to lack of knowledge of its cultivation method, which allows us to affirm that roots and tubers have a primordial place in the nimba zone. The adaptation of these crops to the difficult conditions of the area, their nutritional energy value and their economic contribution deserve the encouragement of these crops despite the difference in yield between these four crops. So for the development of these cultures, authorities at all levels, NGOs, businesses etc. must strengthen the technical and financial capacity of producers, by forming groups or cooperatives, providing them with improved seeds and inputs.

Comparative Study of Ginger Cultivars under Shading Conditions in a High Tunnel

Ginger (Zingiber officinale Rosc.) is a valuable sciophyte crop used as a spice or fresh herb in culinary dishes and for treating medical issues such as osteoarthritis, neurological diseases, vomiting, and asthma. The demand for ginger in the United States is remarkably high; it is produced commercially and exclusively in Hawaii but can only meet ∼20% of US demand. Light for ginger growth may be more important than is often assumed, but the roles of light in ginger growth and rhizome yield are not fully understood. We hypothesized that artificial shade would produce the highest yielding ginger compared with no shade. This study evaluated the impact of different shading suited for ginger growth and rhizome yield of different cultivars grown in a high tunnel. There were three levels (0%, 22%, and 40%) of shade using RCBD design. We analyzed the overall yield (weight) of ginger and the specific yield (weight) of different rhizome parts (biological root, edible root, and seed ginger) per plant in addition to plant growth data. Data were analyzed for 2018 and 2019, and shade conditions influenced ginger growth and yield. There was no significant difference between shade conditions or cultivars, but general trends found that data differed between the two growing seasons. In 2018, Chinese White and Hawaii Yellow produced a better (higher) yield under 0% and 40% shade. However, in 2019, Chinese White and Hawaii Yellow produced the highest total yield under 22% shade, but Bubba Blue produced the highest overall yield at 0% shade.

Starch from Colocasia esculenta (L.) Schott of purple and white esculenta varieties: Thermal, technological properties, and morphological study

The high demand for starch in the food industry drives the search for new alternative sources for extraction. In this regard, Colocasia esculenta (L.) Schott, an edible root, is a promising alternative source for starch extraction. This study focused on correlating the technological and thermal properties with the microstructure and size distribution of starch from white and purple varieties. Starch granules with high thermal stability (132-258.3 °C) and good digestibility based on granule size (0.79-4.05 µm) were obtained. It was demonstrated that larger starch granules exhibit higher water absorption capacity (WAC) (139±0.53%). Moreover, the increase in WAC results in a higher gelatinization temperature (76.1±0.3 °C), which is favorable as it allows the use of this starch in food processing at high temperatures.

Productivity and economics of carrot (Daucus carota) as influenced by interactive effect of potassium and micronutrients

Deficiency and insufficient nutrients application are the major factor responsible to reduced carrot root yield. Nutrients in balanced amount are critical aspect for getting optimal root yield for a particular agro-climatic condition. An appropriate nutrient management including potassium and micronutrients might rise and improve the productivity of carrot. The experiment was conducted at Sri Karan Narendra Agriculture University, Jobner, Jaipur, Rajasthan to study the effect of variable potassium and micronutrients formulations on performance of carrot. Results were found to be significant for the yield attributes, yield and economics of carrot. Soil application of potassium (MOP) @150 kg/ ha significantly enhanced the average root weight (189.3 g), root diameter (3.7 cm), shoot: root ratio (0.5), edible root length (23.7 cm), root yield (28.3 t/ha), net returns (₹205161/ha) and B:C ratio (2.7) of carrot. Among the micronutrients formulations, significantly higher average root weight (182.8 g), root diameter (3.7 cm), shoot: root ratio (0.5), edible root length (24.2 cm), root yield (27.8 t/ha), net returns (₹202576/ha) and B:C ratio (2.7) were noticed in foliar application of ZnSO4 @0.5%. Results of interaction shown that soil application of potassium (MOP) @150 kg/ha and ZnSO4 @0.5% significantly better in the average root weight (209.6 g), root yield (31.4 t/ha), net returns (₹2,35,918/ha) and B:C ratio (3.03).

Biochemical characterisation of a cassava (Manihot esculenta crantz) diversity panel for post-harvest physiological deterioration; metabolite involvement and environmental influence

Cassava (Manihot esculenta Crantz) produces edible roots, a major carbohydrate source feeding more than 800 million people in Africa, Latin America, Oceania and Asia. Post-harvest physiological deterioration (PPD) renders harvested cassava roots unpalatable and unmarketable. Decades of research on PPD have elucidated several genetic, enzymatic and metabolic processes involved. Breeding populations were established to enable verification of robust biomarkers for PPD resistance. For comparison, these PPD populations have been cultivated concurrently with diversity population for carotenoid (β-carotene) content. Results highlighted a significant variation of the chemotypes due to environmental factors. Less than 3% of the detected molecular features showed consistent trends between the two harvest years and were putatively identified as phenylpropanoid derived compounds (e.g. caffeoyl rutinoside). The data corroborated that ∼20 μg β-carotene/g DW can reduced the PPD response of the cassava roots to a score of ∼1. Correlation analysis showed a significant correlation of β-carotene content at harvest to PPD response (R2 -0.55). However, the decrease of β-carotene over storage was not significantly correlated to initial content or PPD response. Volatile analysis observed changes of apocarotenoids derived from β-carotene, lipid oxidation products (alkanes, alcohols and carbonyls and esters) and terpenes. The majority of these volatiles (>90%) showed no significant correlation to β-carotene or PPD. Observed data indicated an increase (∼2-fold) of alkanes in varieties with β-carotene >10 μg/g DW and a decrease (∼60%) in varieties with less β-carotene. Fatty acid methyl esters with a chain length > C9 were detected solely after storage and show lower levels in varieties with higher β-carotene content. In combination with correlation values to PPD (R2 ∼0.3; P-value >0.05), the data indicated a more efficient ROS quenching mechanism in PPD resistant varieties.

Revealing Molecular Mechanisms of the Bioactive Saponins from Edible Root of Platycodon grandiflorum in Combating Obesity.

Obesity has emerged as a significant health concern, as it is a disease linked to metabolic disorders in the body and is characterized by the excessive accumulation of lipids. As a plant-derived food, Platycodon grandiflorum (PG) was reported by many studies, indicating that the saponins from PG can improve obesity effectively. However, the anti-obesity saponins from PG and its anti-obesity mechanisms have not been fully identified. This study identified the active saponins and their molecular targets for treating obesity. The TCMSP database was used to obtain information on 18 saponins in PG. The anti-obesity target of the PG saponins was 115 targets and 44 core targets. GO and KEGG analyses using 44 core anti-obesity genes and targets of PG-active saponins screened from GeneCards, OMIM, Drugbank, and DisGeNet showed that the PI3K-Akt pathway, the JAK-STAT pathway, and the MAPK pathway were the major pathways involved in the anti-obesity effects of PG saponins. BIOVIA Discovery Studio Visualizer and AutoDock Vina were used to perform molecular docking and process the molecular docking results. The molecular docking results showed that the active saponins of PG could bind to the major therapeutic obesity targets to play an obesity-inhibitory role. The results of this study laid the foundation for further research on the anti-obesity saponins in PG and their anti-obesity mechanism and provided a new direction for the development of functional plant-derived food. This research studied the molecular mechanism of PG saponins combating obesity through various signaling pathways, and prosapogenin D can be used to develop as a new potential anti-obesity drug.

Determination of extractable pollutants from microplastics to vegetables: Accumulation and incorporation into the food chain

The presence and impacts of microplastics (MPs) are being extensively researched and reviewed, especially in the marine environment. However, mobility, transportation routes, and accumulation of leaching compounds such as additives in plastic waste including MPs are scarcely studied. Information regarding ecotoxicity and leachability of compounds related to MPs contamination in the environment is limited. Current work presents the levels of leachates from plastic materials in edible-root and non-edible root vegetables. Samples were analyzed by static headspace and gas chromatography-mass spectrometry (SHS-GC-MS) and the presence of 93 putative compounds was accurately monitored in the samples by the usage of Mass Spectrometry-Data Independent Analysis software. The application of chemometrics to the SHS-GC-MS dataset allowed differentiation between the levels of plastic related compounds in edible root and non-edible root vegetables, the former showing a higher content of plastic leachates. For SHS sampling, 3 g of the sample were incubated at 130 °C for 35 min in the HS vial and toluene and naphthalene were added as internal standards for quantification purposes. The developed SHS-GC-MS methodology is straightforward, reliable, and robust and allowed the quantification of sixteen plastic associated compounds in the samples studied in a range from 0.14 to 28800 ng g−1 corresponding to 2,4-di-tert-butylphenol and p,α-dimethylstyrene, respectively. Several of the quantified compounds pointed out to potential contamination of polystyrene and/or polyvinyl chloride MPs.

Selection of inbreds with better combining ability is instrumental in developing CMS-based heterotic hybrids in tropical carrot (Daucus carota L.)

Abstract Carrot is an important vegetable crop worldwide valued for its fleshy edible roots of varied colours. Owing to its highly cross-pollinated nature and small flower size, cytoplasmic male sterility (CMS) is being utilized for hybrid development. Among different types of male sterility, petaloid CMS is widely used for hybrid carrot breeding globally. This study aimed to develop selection criteria for parents in developing heterotic F1 hybrids using CMS lines. A large number of agro-morphological traits and Simple Sequence Repeats (genomic-SSRs) were used to assess the diversity among parental lines. We developed 60 F1 hybrids by crossing four petaloid CMS lines and 15 testers in line × tester mating design and evaluated them in replicated randomized block design trial for four vegetative and 11 economic traits. The mean squares of all the traits in line × tester interactions were significant. The estimates of genetic components of variance indicated predominance of non-additive gene action except for root maturity, root length and core diameter. The hybrids with highest per se performance also had significant positive specific combining ability effects. The root yield and root weight showed highest heterosis percentage (33%). The best performing heterotic hybrids were DCatH-5392, DCatH-700 and DCatH-9892. Correlation between genetic distance and relative heterosis of economic traits indicated no significant association and thus genetic distance could not be used to predict heterosis. As most of the yield-related traits were controlled by non-additive gene action, heterosis breeding could be potentially used along with combining ability analysis to reduce time in selection of best parents and crosses in tropical carrot.

Nutritional evaluation and bioactive compounds of flours of unconventional edible roots

The bakery sector has increasingly sought flour alternatives, such as unconventional edible root flours, with technologically viable characteristics for industrial production, with greater nutritional value and pleasing consumers in their sensory aspects. So, this work aims to evaluate the nutritional value and bioactive compounds of unconventional edible root meals: purple yam (Dioscorea alata), Jerusalem artichoke (Helianthus tuberosus) and ariá (Calathea allouia). Moisture, protein and ash were determined by standardized bromatological methods, lipids by the Bligh-Dyer method, total crude fiber (TGF) by means of the Fiber Analyzer-Ankom 220 device and carbohydrates by difference. The determination of the total phenolic compounds and tannins in the samples were performed using colorimetric analysis methods. A significant variation (p<0.05) was observed between all samples for the humidity (8.31 to 9.37%); proteins (7.47 to 8.93%); ash (3.15 to 4.42%); lipids (0.13 at 1.76%); fiber (0.92 to 4.85%) and carbohydrates (71.52 to 78.18%). For the bioactive compounds, the variation of total phenolics was from 7.35 to 100.77 mg EAG/100g, and for the tannins 0.28 to 1.41 mg EAT /100g. The study showed that the analyzed samples have significant nutritional value, as well as bioactive compounds that can be used in food product formulations.

The Phytochemical Screening and Biological Properties of Brassica napus L. var. napobrassica (Rutabaga) Seeds.

Rutabaga, also known as swede and scientifically classified as Brassica napus napobrassica, is a biennial edible root vegetable that belongs to the Brassica genus and is widely cultivated in North Europe and North America. The present study highlights both the phytochemical profile and the in vitro biological properties of rutabaga seed extracts obtained through maceration using solvents of increasing polarity, namely, cyclohexane (CYHA), dichloromethane (DCM), ethyl acetate (EtOAc), methanol (MeOH), and water (H2O). HPLC-DAD was used to identify and quantify phenolic compounds, while volatile compounds were detected using GC-MS. The in vitro antioxidant capacity of the rutabaga seed extracts was evaluated through DPPH free radical scavenging activity. The in vitro anti-inflammatory activity (15-lipoxygenase (15-LOX) enzyme) was determined spectrophotometrically at the same concentration. Additionally, the cytotoxicity of the seed extracts was evaluated against human colon adenocarcinoma cells (Caco-2) and human embryonic kidney cells (HEK-293) using the MTT assay. The rutabaga seed extracts obtained from EtOAc, MeOH, and H2O were particularly rich in reducing sugars, ranging from 189.87 to 473.75 mg/g DW. The MeOH extract displayed the highest concentration of both sugars and polyphenols. Phytochemically, the HPLC-DAD analysis revealed the presence of four phenolic compounds in the tested extracts, including (±) synephrine, gallic acid, p-coumaric acid, and trans-ferulic acid, newly discovered in rutabaga organs. Moreover, a total of ten volatile compounds were identified through GC-MS analysis, both before and after derivatization. At a concentration of 50 µg/mL, the methanol extract exhibited high antioxidant activity with 52.95% inhibition, while CYHA, DCM, and EtOAc exhibited moderate anti-15-LOX activity with less than 30% inhibition. Except for DCM and aqueous extracts, rutabaga seeds did not exhibit any anti-proliferative potential against Caco-2 cell lines. Interestingly, no cytotoxicity was registered for any of the seed extracts against the normal cell line HEK-293. Overall, the obtained data highlight the potential utilization of rutabaga seeds as a source of bioactive compounds in various fields, including pharmaceuticals, nutraceuticals, and functional foods.

Effectiveness of mycorrhizal inoculants on sweet potato (Ipomoea batata) in Eutric Cambisol soils of Cuba

Introduction. The effective use of mycorrhizal inoculants is a challenge for Cuban agriculture. Sweet potato is an important crop for human and animal nutrition, being a mycorrhizal dependent crop with a successful breeding program. Objectives. To know if all sweet potato cultivars respond to inoculation and if its effectiveness varies with cultivars and growing seasons. Materials and methods. Two experiments were carried out during 2010 - 2012 on Eutric Cambisol soils from Villa Clara, Cuba. One at each planting time and repeated twice. The response of 17 cultivars to three inoculants application in the presence of a half dose of fertilization, and three non-inoculated treatments with fertilization levels of 0, 50, and 100 % of nitrogen, phosphorus, and potassium dose (100 % NPK) was evaluated, using a split plot design. Edible root yields, mycorrhizal colonization frequency and spores were evaluated as response variables. Results. Cultivars responded positively to inoculation and fertilization, showing differences in their yields, however, highest yields were always found when inoculated with Rhizoglomus irregulare/ INCAM-11, which were higher (p<0.05) than those obtained when receiving only 50 % NPK dose. In the rainy season with higher yields, the differentiation between inoculants was higher and in thirteen and nine of the cultivars, the yields obtained with INCAM-11 were higher (p<0.05) than those obtained with Glomus cubense/ INCAM-4 and with 100 % NPK respectively. In the low rainy season, no significant differences were found between yields obtained by inoculating INCAM-11 or INCAM-4 or applying 100 % NPK. In both seasons, inoculation with Funneliformis mosseae/ INCAM-2 was always lower. Colonization frequencies and spore production were always higher (p<0.05) when inoculating INCAM-11. Conclusion. Under these soil conditions, INCAM-11 inoculation presented better effectivity for all cultivars and planting seasons originating the highest yields and indicators of mycorrhizal performance.

Biosynthesis of ZnO and TiO2 nanoparticles using Ipomoea carnea leaf extract and its effect on black carrot (Daucus carota L.) cv. Pusa Asita

Nano fertilizers (NFs) are now becoming an important tool for plant nutrient management having capabilities to improve soil fertility, crop productivity and quality of agricultural products. Since, they are needed in very small amount, thus, reduces cost of crop production. Among different essential or beneficial plant nutrients, Zn and Ti are important micro nutrients having number of beneficial effect on crop growth, yield, quality and post harvest life. Present experiment was carried out to prepare ZnO and TiO2 nanoparticles (NPs) through green technology by using aqueous extract of Ipomoea carnea (morning glory) leaves. In order to investigate size, morphology, composition, and stability of selected NPs, the detailed characterization was carried out using UV–visible spectroscopy, FTIR, HRTEM, EDX, BET, X-ray diffraction, XPS and particle size distribution studies. Subsequently, the effect of foliar spray of ZnO and TiO2 NPs was evaluated in respect of vegetative growth, yield and quality of black carrot (Daucus carota L.) cv. Pusa Asita in presence of 50% Recommended dose of fertilizer (RDF) to assess their effect on fertilizer use efficiency also. There were 8 treatments viz. Control (no fertilizer), recommended dose of fertilizer (RDF), TiO2 (5, 10 and 15 ppm along with 50% RDF), ZnO (50, 75 and 100 ppm along with 50% RDF)] with 3 replications following Randomised Block Design having 24 plots (1 m × 1 m). The observations were taken for vegetative growth, edible root yield and root quality parameters. Although, the growth, yield and quality parameters were found superior (root yield 43.84 g/plant) under conventional system of recommended dose of fertilizers (RDF) of NPK, however, TiO2 NPs also showed very promising result close to RDF as compared to ZnO NPs. Among them, 5 ppm TiO2 foliar application along with 50% NPK was found to be the best in terms of vegetative growth, root yield (38.73 g/plant) and quality of black carrot. It was also found that higher concentration of TiO2 and ZnO NPs had adverse effect on the plant performance. Therefore, it can be concluded that 5 ppm TiO2 NPs along with 50% RDF was good for black carrot production.

자리공 중독 사망 사례

Phytolacca has been used as food and folk medicines for rheumatism and arthritis in America and East Asia. All parts of it can be considered toxic unless it is properly prepared. Some acute intoxication cases occurred by taking Phytolacca without proper treatment, or accidently misusing it as edible roots. Poisoning by Phytolacca can cause minor digestive trouble such as vomiting, abdominal cramps and diarrhea. But in some cases, it can result serious symptoms like seizures, acute kidney failure and even death. The roots of Phytolacca are similar to other edible roots, so they can be mistakenly ingested. In this study, we presented a fatal post-mortem intoxication case of Phytolacca poisoning. The heart and peripheral blood, gastric contents were collected from autopsy, and the ingested plant roots from the scene submitted for toxicological analysis. The detection of triterpenoid saponins in biological samples and plant materials were performed by solid-phase extraction (SPE) followed by liquid chromatography-mass spectrometry (LC-MS/MS). The concentrations of esculentoside A in the heart and peripheral blood were 37 ng/mL and 27 ng/mL, respectively, while those of esculentoside H were 3 ng/mL and 1 ng/mL, respectively.

Fabrication of magnetic magnesium oxide cleanup adsorbent for high-throughput pesticides residue analysis coupled with supercritical fluid chromatography-tandem mass spectrometry

A rapid and accurate analytical method was established for multiple pesticide residues in complex matrices based on magnetic dispersive solid phase extraction (d-SPE) and supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS). To develop an efficient magnetic d-SPE method, magnetic adsorbent modified with magnesium oxide (Fe3O4–MgO) was prepared via layer-by-layer modification and used as cleanup adsorbent for removal of interferences that contain a large number of hydroxyl or carboxyl groups in the complex matrix. The obtained Fe3O4–MgO coupled with 3-(N,N-Diethylamino)-propyltrimethoxysilane (PSA) and octadecyl (C18) were used as d-SPE purification adsorbents and their dosages were systematically optimized with Paeoniae radix alba as the matrix model. Combined with SFC-MS/MS, rapid and accurate determination of 126 pesticide residues in the complex matrix was achieved. Further systematic method validation showed good linearity, satisfactory recovery, and wide applicability. The average recoveries of the pesticides at 20, 50, 80, and 200 μg kg−1 were 110, 105, 108, and 109%, respectively. The proposed method was applied to complex medicinal and edible root plants, such as Puerariae lobate radix, Platycodonis radix, Polygonati odorati rhizoma, Glycyrrhizae radix, and Codonopsis radix. The average recoveries of the pesticides at 80 μg kg−1 in these matrices were 106, 106, 105, 103, and 105%, respectively with an average relative standard deviation range of 8.24–10.2%. The results demonstrated the feasibility and wide matrix applicability of the proposed method, which is promising for pesticide residue analysis in complex samples.

A Review on Quality Scanner Device for Radish and Carrot using Image Processing Technique

Abstract: Agriculture is the backbone of the Indian economy and productivity. The market value obtained from them is immense and effect both the export and quality valuation . Although grading and quality can be analysed by humans it is still inaccurate, time intensive, unreliable, subjective, and also hard which can be easily influenced by surrounding. Hence, there is an ultimate need for intelligent classification for vegetables. This paper focuses on Developing an analysis device for Radish (Raphanus raphanistrum ) and Carrot (Daucus carota subsp. sativus) which is an edible root vegetable grown in most parts of India. Radish and carrots are good sources of natural nitrates, minerals, calcium, and potassium. Thus, vegetables must be used as soon as possible to get a superior fresh character from them. External parameters such as size, shape, and quality factors of radish are important for finding out the total quality that is acceptable to the people. Various methods and algorithms are been put forwarded for the quality evaluation for the external defects present in it. Convolutional Neural Networks is the method used in image processing as this is more advantageous since it can learn special characteristics from images and by establish CNN for image classification where analyst have to gather the dataset. The requirement for doing this project is to have a computer and a python program for use the driver code. Image processing method is used for estimating quality of the particular food product efficiently in these days. This review paper examines current developments in methods for assessing food quality through image processing. The use of CNN (Convolutional Neural Networks) to identify characteristics of vegetables and its diseases through several built-in python libraries

Morphological characterizations of parsnip (Pastinaca sativa L.) to select superior genotypes.

Parsnip (Pastinaca sativa L.) is an edible root that has long been used in cooking and preparing baby food and livestock. The present study was performed to evaluate the phenotypic diversity of 69 accessions of this species to select superiors in terms of root quality in Paykan village, Isfahan province, Iran, in the year 2022. There were significant differences among the accessions investigated (ANOVA, p < .01). Coefficient of variation (CV) was more than 20.00% in the majority of measured characters (64 out of 66 characters), indicating high diversity among the accessions. Foliage width (crown) ranged from 10 to 55 cm with an average of 32.32 cm. Root shape was tapering (33), obtriangular (10), narrow oblong (5), wide oblong (5), obovate (13), and fusiform (3). Root length ranged from 81.2 to 294 mm with an average of 166.44 mm. Root diameter at its middle point ranged from 15.58 to 125.12 mm with an average of 51.83 mm. Root weight ranged from 15 to 1200 g with an average of 315.36 g. Inner core (xylem) pigmentation/color was cream yellow (11 accessions), light yellow (12), yellow (42), dark yellow (2), and yellow-light orange (2). In the cluster analysis based on Ward's method, the accessions were divided into two main clusters according to morphological traits. This is despite the fact that parsnip is part of the medicinal plant native and valuable in most farms in tropical cities. Compared with carrots, parsnip plants are more adaptable to different environmental conditions. The accessions studied here showed high phenotypic diversity. Based on ideal values of the important and commercial characters of parsnip, such as root length, root weight, inner core (xylem) pigmentation/color, root shape, flesh color intensity, flesh palatability, and total soluble solids, 14 genotypes, including Parsnip-3, Parsnip-9, Parsnip-24, Parsnip-32, Parsnip-32, Parsnip-48, Parsnip-51, Parsnip-52, Parsnip-58, Parsnip-60, Parsnip-62, Parsnip-65, Parsnip-67, and Parsnip-69, were promising and are recommended for cultivation.

A systematic antidiarrhoeal evaluation of a vegetable root Begonia roxburghii and its marker flavonoids against nonpathogenic and pathogenic diarrhoea

The plant Begonia roxburghii, widely distributed in North East India, is regularly used as a vegetable and food item in these areas. The plant's roots are traditionally used in treating digestive disorders, diarrhoea, dysentery and various stomach ailments. Therefore, the main objective included scientific justification of the traditional assertions about the edible roots and its marker flavonoids from the plant in the treatment of diarrhoea, followed by deriving its mechanism. HPLC was used to standardize the ethanolic extract of B. roxburghii (EBR) with respective markers luteolin and rutin. The study revealed the similar antibacterial potential of both the markers (luteolin and rutin); however, normal faecal excretion showed the highest antisecretory potential of EBR followed by rutin. EBR at 300 mg/kg, p.o. demonstrated the highest % protection from diarrhoea in both nonpathogenic (castor oil-induced) and pathogenic (enteropathogenic E. coli-induced) diarrhoea rat models, which also restored the altered biochemical parameters, ion concentration and cytokine levels. EBR and rutin also justified their protective nature through histopathology showing less broadening of villus and intact epithelia compared to diarrhoea control rat colons. The in-silico studies validated the contribution of rutin as a major biomarker which enhanced Na+/K+–ATPase activity and stabilized the C-terminal 282-residue fragment of EPEC intimin and was also supported by findings of network pharmacology observations. The present investigation justified the traditional claim of the edible roots of B. roxburghii in treating diarrhoea, where its marker rutin played a significant role in reactivating Na+/K+-ATPase, restored electrolyte balance, and also stabilized the EPEC C-terminal.

The Effect of Utilization of Cassava Solar Drying Technology on small – Scale Cassava Farmers Incomes’ in Teso-South Sub-County of Busia County, Kenya

Cassava (with a Botanical name Manihot esculenta) is a perennial woody shrub with an edible root, which is grown in tropical and subtropical parts of the world, including sub-Saharan Africa. Cassava is grown in the former Western Province, which constitutes Kakamega, Bungoma, and Busia Counties, and has huge potential for food security. The challenges the farmers face is a lack of proper handling during harvesting which they spoil easily and hence sell it raw, making cultivation a business unsustainable due to heavy losses. This paper was on effect of utilization of cassava solar drying technology on small-scale cassava farmers’ incomes’ in Teso-South Sub-County of Busia County, Kenya. Primary data was collected using a structured questionnaire and analysis was done through SPSS where descriptive and inferential statistics. The findings showed that utilization of cassava solar drying technology (β=0.137, p&lt;0.05) was a significant predictor of small-scale cassava farmers’ incomes in Teso-South Sub County, Busia County, Kenya. The study recommends that government of Busia County should put in place and strengthen the adult literacy programs, invest in acquisition of solar dryers, and training on dryer usage among small-scale cassava in Busia County.

Utility of Ugandan genomic selection cassava breeding populations for prediction of cassava viral disease resistance and yield in West African clones.

Cassava (Manihot esculenta Crantz) is a staple crop for ~800 million people in sub-Saharan Africa. Its production and productivity are being heavily affected by the two viral diseases: cassava brown streak disease (CBSD) and cassava mosaic disease (CMD), impacting greatly on edible root yield. CBSD is currently endemic to central, eastern and southern Africa, if not contained could spread to West Africa the largest cassava producer and consumer in the continent. Genomic selection (GS) has been implemented in Ugandan cassava breeding for accelerated development of virus resistant and high yielding clones. This study leveraged available GS training data in Uganda for pre-emptive CBSD breeding in W. Africa alongside CMD and fresh root yield (FRW). First, we tracked genetic gain through the current three cycles of GS in Uganda. The mean genomic estimated breeding values (GEBVs), indicated general progress from initial cycle zero (C0) to cycle one (C1) and cycle two (C2) for CBSD traits and yield except for CMD. Secondly, we used foliar data of both CBSD and CMD, as well as harvest root necrosis and yield data to perform cross-validation predictions. Cross-validation prediction accuracies of five GS models were tested for each of the three GS cycles and West African (WA) germplasm as a test set. In all cases, cross-validation prediction accuracies were low to moderate, ranging from -0.16 to 0.68 for CBSD traits, -0.27 to 0.57 for CMD and -0.22 to 0.41 for fresh root weight (FRW). Overall, the highest prediction accuracies were recorded in C0 for all traits tested across models and the best performing model in cross-validation was G-BLUP. Lastly, we tested the predictive ability of the Ugandan training sets to predict CBSD in W. African clones. In general, the Ugandan training sets had low prediction accuracies for all traits across models in West African germplasm, varying from -0.18 to 0.1. Based on the findings of this study, the cassava breeding program in Uganda has made progress through application of GS for most target traits, but the utility of the training population for pre-emptive breeding in WA is limiting. In this case, efforts should be devoted to sharing Ugandan germplasm that possess resistance with the W. African breeding programs for hybridization to fully enable deployment of genomic selection as a pre-emptive CBSD breeding strategy in W. Africa.

Earthworms Drive the Effect of La2O3 Nanoparticles on Radish Taproot Metabolite Profiles and Rhizosphere Microbial Communities.

To promote the sustainable and safe application of nanotechnology employing engineered nanoparticles (NPs) in agroecosystems, it is crucial to pay more attention to the NP-mediated biological response process and environmental impact assessment simultaneously. Herein, 50 mg kg-1 La2O3 NPs were added to soils without and with earthworms for cherry radish growth for 50 days to investigate the response changes of metabolites in radish above- and below-ground organs and rhizosphere bacterial communities. We found that La2O3 NP exposure, especially with earthworms, notably increased the La bioavailability and uptake by taproots and eventually increased radish leaf sucrose content and plant biomass. The La2O3 NP exposure significantly altered metabolite profiles in taproot flesh and peel tissues, and particularly La2O3 NP exposure combined with earthworms was more conducive to La2O3 NPs to promote radish taproot peel to synthesize more secondary antioxidant metabolites. Moreover, compared with the control, the La2O3 NP exposure resulted in weaker and fewer correlations between rhizosphere bacteria and taproot metabolites, but this was recovered somewhat after the inoculation of earthworms. Altogether, our results provide novel insights into the soil-fauna-driven biological and biochemical impact of La2O3 NP exposure on edible root crops and the long-term environmental risks to the rhizosphere microbiota in agroecosystems.