The effect of silver nanoparticles (AgNPs) in concentrations was evaluated 0.1; 0.5; 1; 5; 10; 50 and 100 mg/kg for the germination and root length of ordinary black earth radish 30 days after contamination. It was found that the degree of ecotoxic effect of AgNPs on the germination and length of radish roots is directly dependent on their dose in the soil. The content of AgNPs 1, 5, 10, 50, 100 mg/kg in ordinary chernozem caused a decrease in the germination of radish by 11, 13, 15, 18 and 38 % compared with the values in uncontaminated soil. Doses of AgNPs 0.5, 1, 5, 10, 50 and 100 mg/kg inhibited the length of radish roots by 19, 20, 25, 30, 37 and 43 % compared with the values obtained in uncontaminated soil. The radish root length index showed the greatest sensitivity to soil contamination by AgNPs compared to the germination index. The obtained results on the assessment of phytotoxicity of soils contaminated with AgNPs can be used for diagnostics and as an indicator of the ecological state of soils.