It is important to capture passengers’ public transit behavior and their mobility to create profiles, which are critical for analyzing human activities, understanding the social and economic structure of cities, improving public transportation, assisting urban planning, and promoting smart cities. In this paper, we develop a generative adversarial machine learning network to characterize the temporal and spatial mobility behavior of public transit passengers, based on massive smart card data and road network data. The Apriori algorithm is extended with spatio-temporal constraints to extract frequent transit mobility patterns of individual passengers based on a reconstructed personal trip dataset. This individual-level pattern information is used to construct personalized feature vectors. For regular and frequent public transit passengers, we identify similar transit mobility groups using spatio-temporal constraints to construct a group feature vector. We develop a generative adversarial network to embed public transit mobility of passengers. The proposed model’s generator consists of an auto-encoder, which extracts a low-dimensional and compact representation of passenger behavior, and a pre-trained sub-generator containing generalization features of public transit passengers. Shenzhen City is taken as the study area in this paper, and experiments were carried out based on smart card data, road network data, and bus GPS data. Clustering analysis of embedding vector representation and estimation of the top K transit destinations were conducted, verifying that the proposed method can profile passenger transit mobility in a comprehensive and compact manner.