Fish Meal Research Articles

The Bioavailability of Solid-State Fermented Feather Meal Using a Novel Feather-Degrading Bacterium Bacillus velezensis PN1 in Broilers

In this study, an effective feather-degrading bacterium was isolated and the solid-state fermentation condition for feather degradation was optimized. The resulting fermented feather meal (FFM) was evaluated for its bioavailability in broilers. Four Bacillus strains were examined for feather degradation rates, with Bacillus velezensis PN1 exhibiting the highest rate (83.24%, p < 0.05). A 3 × 3 × 3 factorial design was used to test substrate moisture content (45%, 55%, 65%), temperature (27 °C, 37 °C, 47 °C), and incubation time (24, 48, 72 h) for optimized fermentation conditions. In vitro pepsin digestibility (IVPD) revealed a significant interaction between temperature and time (p < 0.05), and the optimal performance was achieved at 37 °C for 72 h, followed by 37 °C for 48 h. Considering production time and cost, FFM2 (produced with 65% moisture at 37 °C for 48 h) was further compared with FFM1 (produced under the same conditions but at a lower temperature of 27 °C), and commercial hydrolyzed feather meal (HFM). IVPD did not differ significantly between FFM1, FFM2, and HFM, as they all showed significantly higher digestibility compared to raw feathers (RFs). FFM1, as well as FFM2, had significantly higher histidine and lysine concentrations than RF (p < 0.05), while FFM2 had a significantly lower cysteine content (p < 0.05). Based on fermentation conditions and amino acid composition, FFM2 was considered more suitable for large-scale production and was used in a broiler growth trial. The inclusion of 5% FFM2 in the broiler diet did not significantly affect body weight at 35 days compared to the 5% HFM group (p > 0.05), although both groups showed significantly lower weight gain than the 5% fish meal (FM) group (p < 0.05). The feed conversion ratio and performance efficiency factor did not differ significantly between the FFM2, HFM, and FM groups (p > 0.05). In groups fed diets without supplemental crystalline amino acids, growth performance did not significantly differ between the HFM and FFM2 groups (p > 0.05), although both performed significantly worse than groups with amino acid supplementation. In conclusion, FFM produced by B. velezensis PN1 through solid-state fermentation enhances feather bioavailability in poultry and can completely replace HFM when included at 5% in broiler diets, offering a potential sustainable alternative for poultry nutrition on a commercial scale.

Abstract 4135338: Associations between plant-based diets and total mortality among Chinese older participants with cardiometabolic diseases: a prospective cohort study

Background and Aims: Plant-based diets have been associated with a lower risk of total mortality among generally health populations. However, the associations of plant-based diets with total mortality remain unclear among older people with cardiometabolic diseases, who have an elevated risk of mortality. Methods: We prospectively followed 4,485 participants (mean age: 84 years) from the China Prospective Longitudinal Survey of Health and Longevity (2006-2018), who had cardiometabolic disease in 2006. Dietary data were collected at baseline using a food-frequency questionnaire. Using these data, we created an overall plant-based diet index (PDI), in which plant-based foods received positive scores while animal foods (meat, fish and aquatic products, eggs, milk, animal fats) received reversed scores accordingly. We also created a healthful plant-based diet index (hPDI), where healthy plant-based foods (whole grains, vegetables, vegetable oils, fresh fruits, legumes, beans, garlic, nuts, tea) received positive scores, while less healthy plant-based foods (refined grains, pickles, sugar, preserved vegetables) and animal-based foods were given reversed scores. In addition, an unhealthful plant-based diet index (uPDI) was created by assigning positive scores to less healthy plant-based foods while reverse scores to healthy plant-based foods and animal-based foods. We used Cox proportional hazards regression to compute hazard ratios (HRs) and 95% CIs for total mortality, with adjustment for demographics, dietary and lifestyle factors, and medical history. Results: During a 12 years of follow-up, 2,881 deaths were recorded. A higher score from PDI and hPDI was associated with a lower risk total mortality among participants with cardiometabolic diseases (HR and 95% CI for extreme quintiles of PDIs: 0.85, 95% CI 0.75–0.96, P trend < 0.001; hPDI: 0.84 (0.74–0.94), P trend < 0.001). A higher uPDI score was associated with a higher total mortality among participants with cardiometabolic diseases (uPDI: 1.28 (1.13-1.47), P trend < 0.001). These results were consistent in subgroup analyses by age, sex, smoking status, alcohol consumption, education level, physical activity, and BMI. Conclusion: Our study suggests that among older Chinese participants with cardiometabolic diseases, adherence to an overall plant-based diet or a healthful plant-based diet, is associated with a lower total mortality, while an unhealthful plant-based diet was associated with a higher total mortality.

Formaldehyde as Preservative for Fish and Seafood: A Boon or a Bane

ABSTRACTThree billion people globally rely on fish and other seafood as a significant source of animal protein. Despite being deemed illegal due to its ability to cause cancer, mutagenesis, and tumor development, formaldehyde is still in use to extend the shelf life of different fish and shellfish, which indicates the failure of awareness among people. Formaldehyde is categorized by the International Agency for Research on Cancer (IARC) as a Group‐I human carcinogen. Therefore, we try to summarize the chemistry of formaldehyde, its illegal addition in various food materials leading to adverse health conditions followed by its evaluation at physiological and histological levels in our review with the help of data collected from various research papers, newspaper articles, and online data. We have also highlighted on various environmental factors that play a role in altering the levels of formaldehyde in various food materials. This review also gives an insight into various rules and regulations made to limit the usage of formaldehyde in fish and other seafood at both national and international levels. Our review will help to generate awareness among common people, the establishment of much stronger rules, and regulations on usage of formaldehyde, and finding an alternative for preservation of fish and other food materials.

Effects of replacing fish meal with pork meal and supplementing with crystalline amino acids and fish oil on growth performance, body composition, enzyme activity, intestinal morphology, and immune response of Penaeus monodon

IntroductionThe present study aimed to evaluate the effects of substituting fish meal with pork meal in feed on the growth performance, feed utilization, intestinal morphology, and immune function of Penaeus monodon.MethodsA total of 600 uniformly sized juvenile shrimp with an initial weight of 1.20 ± 0.03 g were randomly assigned to four groups, with each group consisting of six replicates of 25 shrimp each. Fish meal protein was replaced with 0%, 5%, 10%, and 15% pork meal, and crystalline amino acids and fish oil were supplemented accordingly. The groups were designated as P0, P5, P10, and P15, and the study duration was 70 days.ResultsResults indicated no significant difference in specific growth rate (SGR) between the P5 and P10 groups compared to the P0 group. However, the SGR in the P15 group was significantly lower than that in the P5 and P10 groups. Feed conversion rates (FCRs) for the P5 and P10 groups were comparable to the P0 group, whereas the P15 group showed a significantly higher FCR than the other groups. There were no significant differences in whole body composition among all the groups. Apart from alanine, the amino acid composition of muscles did not differ significantly among all the groups. Results of enzyme activity in plasma revealed that the replacement of fish meal with pork meal reduced levels of aspartate aminotransferase (AST) and superoxide dismutase (SOD). Specifically, levels of AST in the P5 and P15 groups were significantly lower compared to the P0 and P10 groups. Histological examination of midgut tissues showed that villus height initially increased and then decreased with higher replacement ratios. The P5 group had the highest villus height, while the P15 group had the lowest villus height. Analysis of gene expression related to liver immune functions indicated that genes in TLR signaling pathway were significantly elevated in the P15 group compared to the other groups.DiscussionIn conclusion, even with the supplementation of crystalline amino acids and fish oil, pork meal should not replace more than 10% of fish meal in the feed of P. monodon to maintain optimal growth and feed efficiency.

TLR2/TLR5 Signaling and Gut Microbiota Mediate Soybean-Meal-Induced Enteritis and Declined Growth and Antioxidant Capabilities in Large Yellow Croaker (Larimichthys crocea)

Soybean meal, renowned for its high yield, cost efficiency, and protein richness, serves as a pivotal plant-based alternative to fish meal. However, high soybean meal inclusion in Larimichthys crocea diets is linked to enteritis and oxidative damage, with unknown mechanisms. Our study aims to elucidate the molecular basis of soybean-meal-induced enteritis and its impact on intestinal microbiota in L. crocea. To this end, four isonitrogenous and isolipidic diets with varying soybean meal levels (0% FM, 15% SBM15, 30% SBM30, and 45% SBM45) were administered to L. crocea for 8 weeks. The results indicated that the SBM30 and SBM45 treatments significantly hindered fish growth, digestive efficiency, and protein utilization. Furthermore, high soybean meal levels (SBM30 and SBM45) activated intestinal Toll-like receptors (TLR2A, TLR2B, TLR5, and TLR22), stimulating C-Rel and mTOR protein expression and elevating ERK phosphorylation. This led to increased pro-inflammatory cytokine production (IL-1β, IL-6, and TNF-α) and decreased anti-inflammatory cytokine expression (IL-4/13A, IL-4/13B, and TGF-β), suggesting a potential signaling pathway for soybean-meal-induced enteritis. Furthermore, enteritis induced by high soybean meal levels led to oxidative damage, evident from increased MDA levels and decreased antioxidant enzyme activities (SOD and CAT). The SBM30 and SBM45 treatments increased Firmicutes and Bacteroidetes abundance in fish gut microbiota, while Proteobacteria abundance decreased. This microbiota shift may enhance soybean meal nutrient utilization, yet high soybean meal concentrations still impair growth. A soybean-meal-rich diet promotes harmful bacteria like Rhodococcus and depletes probiotics like Ralstonia, increasing disease risks. L. crocea has limited tolerance for soybean meal, necessitating advanced processing to mitigate anti-nutritional factors. Ultimately, exploring alternative protein sources beyond soybean meal for fish meal replacement is optimal for L. crocea.

A taste for growth: Rapeseed lecithin improves the feed intake of post‐juvenile rainbow trout (Oncorhynchus mykiss)

AbstractFeed formulations low in fish meal and fish oil can decrease feed intake and nutrient digestibility. Dietary application of lecithins could improve growth performance and nutrient digestibility; however, knowledge about specific lecithins and their effects in post‐juvenile fish is lacking. This study evaluated the effects of a commercially available rapeseed lecithin mixture on growth performance, feed intake, and nutrient digestibility of post‐juvenile rainbow trout (77.1 ± 12.1 g). A commercially based diet low in fish meal served as the control, while in the remaining three diets, fish oil was replaced with lecithin by 50%, 100% with lecithin, or 100% with a mixture of equal amounts of lecithin and rapeseed oil. Fish were fed to apparent satiation twice per day for 56 days. At the end of the trial, growth parameters, health parameters, nutrient digestibility, and filet coloration were assessed. Overall growth and feed intake were significantly elevated in all lecithin diets compared with the control group. The nutrient digestibility of all diets containing lecithin was similar to the control, and no differences in health parameters were detected. Filet coloration was significantly improved in diets of 100% fish oil replacement. Rapeseed lecithin can act as a feed attractant in fish meal and fish oil‐reduced diets.

Manures base on Oreochromis niloticus for agricultural use

ABSTRACT Objective: Oreochromis niloticus (Tilapia) meal was evaluated as a source of nutrients in agriculture, through a composting process mixed with a zero tillage soil. Design/methodology/approach: Two manures were formulated, mixing flour and soil at a 30:70 ratio (mass/mass w:w) respectively. Compost A was maintained at 60 days of composting (ddc), compost B at 30 days. The phytotoxicity of the manures was evaluated as percentage of germination in Solanum lycopersicum (tomato) seeds. Results: The N-P-K ratio (% dry mass) was 1.90-2.06-0.30 for manure A (60 ddc), and 2.60-2.24-0.34 for B (30 ddc). On germination, 100% concentration; manure A had 44.9% and B 19.6%, compared to the control. For concentration of 10% it was 93.7 and 111.4%, manure A and B, respectively. Limitations on study/implications: It is necessary to carry out more phytotoxicity tests. In this experiment, dilutions were made in the manures to increase germination. Findings/conclusions: The use of manure based on tilapia meal in agriculture is proposed in the future and in this way take advantage of the waste from fish processing. Keywords: Fish meal, compost, tomato, soil improver

A critical review on heavy metal contamination in aquatic food webs by edible fish species: a special case concerning Bangladesh.

Heavy metals (HMs) are ubiquitous in terrestrial and aquatic environments due to unplanned industrial waste discharge, the release of untreated wastewater, and improper mining activities. In particular, the concentrations of HMs are found to be higher in aquatic environments. As a result, the aquatic biota was heavily affected by HM contamination. This critical review aims to understand the sources and toxicity of HMs in commercial fish species, explore their ecological exchange, and examine the related human health challenges in Bangladesh. A modified PRISMA review technique is used in this paper to analyze the current status and research limitations of HM studies in Bangladesh fish species and their toxicity within aquatic food webs. Briefly, we searched several keywords to explore the research trend of HM concentrations and toxicity in fish species. Furthermore, potential toxicity and risk assessment of HMs through the aquatic food chain in Bangladesh were explored. On the other hand, a cross-tabulation approach was used to process the toxicity findings of HMs. Previous studies indicate that fish species can possess comparatively higher HMs than river water due to ecological exchange factors, including bioaccumulation and biotransformation. This review focuses on Bangladesh, highlighting areas for improvements and the need for further study to achieve a transparent understanding of HM deposition in fish species and the sustainable management of aquatic food chain toxicity.

The impact of quality certificates on sustainable food production: An analysis of selected systems in Poland

This study evaluates the influence of quality certificates on sustainable food production in Poland, considering economic, social, and environmental dimensions. Analyzing 25 different certificates, the research explores their criteria, procedures, and costs across various food product categories, including meat, fish, and plant-based products. The study provides a detailed review of certification processes, from initiation to audits and inspections. It identifies both commonalities and differences among certificates, each addressing unique aspects such as environmental impact, worker rights, and product origins. Despite the diversity in standards and procedures, the study underscores the need for standardized international criteria to improve transparency and meet consumer expectations, highlighting the significant role of quality certificates in advancing sustainable food production.

Effects of fish meal replacement with composite proteins source on growth performance, antioxidant capacity and swim bladder quality of chu’s croaker (Nibea coibor)

Collagen, as a commercial functional protein, is widely applied in food, cosmetics, biomedicine and other industries, and swim bladder is regarded as an ideal source of raw materials due to its rich collagen content. Chu’s croaker (Nibea coibor), as an economical fish that can produce high-grade swim bladder, is popularly farmed, however, its high fishmeal demand limits the development of the feed industry. Therefore, it is worth thinking about how to reduce the use of fish meal without affecting the swim bladder quality. Given that, an 8-week feeding trial was conducted to investigate the effects of fish meal partially replaced (0.0%, 5.0%, 10.0%, 15.0%, 20.0%) with composite proteins (CPs, consisted of corn protein meal and hydrolyzed feather meal) on growth, antioxidant capacity and swim bladder quality (texture and collagen deposition) of chu’s croaker. Results showed that although growth performance decrease with the increase of CPs’ replacement level, it did not compromise the swim bladder quality. Specifically, 5% - 10% CPs did not affect the growth significantly, but can improve antioxidant capacity, protein content, collagen synthesis and texture quality of swim bladder. The enhancement of swim bladder texture, including hardness, chewiness, springiness, and adhesiveness may be caused by changes in the expression of related genes, such as colga1t1, col12a1, cyp27a1, etc. These results indicated that appropriate fish meal replaced by CPs could be applied during aquaculture practice after comprehensive consideration of feed cost, growth rate and quality of swim bladder. This study provides support for the development of cost-effective feed formulations that improve the sustainability and economic viability of chu’s croaker culture.

Effects of Replacing Fish Meal with Rubber Seed Cake on Growth, Digestive, Antioxidant and Protein Metabolism of Juvenile Asian Red-Tailed Catfish (Hemibagrus wyckioides)

An 8-week feeding trial was conducted to assess the impact of replacing fish meal (FM) with rubber seed cake (RSC) on growth performance, digestive enzyme activity of the gut, antioxidant capacity and protein metabolism-related parameters of plasma and liver in juvenile Asian red-tailed catfish (Hemibagrus wyckioides) with an average body weight of 3.21 g. Four isonitrogenous (41.08 ± 0.17) and isolipidic (10.07 ± 0.11) diets were formulated, incorporating RSC at a level of 0%, 15%, 30%, and 45% (w/w) to progressively replace FM. Dietary inclusion of 15–30% RSC did not significantly affect growth performance (p > 0.05). However, the replacement of FM with RSC resulted in linear reductions in intestinal trypsin and amylase activities, with a significant reduction in intestinal amylase activity observed at the 45% RSC inclusion level. Dietary inclusion of 30–45% RSC decreased plasma glutamate dehydrogenase and aspartate aminotransferase activities, but increased plasma adenosine monophosphate deaminase and hepatic γ-glutamyltransferase activities. This may indicate that the high substitution of FM by RSC may affect the homeostasis of amino acid metabolism. Dietary inclusion of 15% RSC did not affect serum and hepatic antioxidant enzyme activities; however, dietary inclusion of 45% RSC significantly increased plasma malondialdehyde level, but decreased catalase activity. Furthermore, dietary inclusion of 30–45% RSC significantly increased hepatic lactate dehydrogenase activity. These results demonstrated that replacing a portion of FM with RSC is feasible, and dietary inclusion of 15% RSC did not have significant adverse effects on growth performance, digestive function and antioxidant capacity of H. wyckioides.

P-type Accumulation Mode Organic Electrochemical Transistor Biosensor for Xanthine Detection in Fish

Food waste is a global challenge that needs to be mitigated in the development of more sustainable societies. From manufacturers to customers, food biosensors could effectively reduce the amount of discarded food and provide more precise predictions of freshness with respect to pre-decided expiration dates. In this study, we developed a novel organic electrochemical transistor (OECT)-based xanthine biosensor. The OECT-based biosensor is based on the p-type conjugated polymer, p(g42T-TT) as the channel, and incorporated xanthine oxidase (XOD) as the biorecognition element. The OECT thus acts as a transducer and amplifier of the enzymatic oxidation of xanthine. Real-time monitoring of xanthine using the OECT-based biosensor led to a linear range between 5 and 98 μM (), 3.28 μM limit of detection, and high sensitivity up to 21.8 mA/mM. Real sample tests showed that the biosensor can detect the accumulation of xanthine in fish meat from 0 to 6 days of degradation. Interference tests with ascorbic acid and uric acid and spike-and-recovery tests with fish samples indicated that as-designed biosensors have good selectivity and accuracy. The developed biosensors show great potential for point-of-care testing applied to food monitoring.

Replacement of fish meal with full fat Hermetia illucens modulates hepatic FXR signaling in juvenile rainbow trout (Oncorhynchus mykiss): exploring a potential role of ecdysteroids

The present study was conducted to investigate the effects of fish meal (FM) replacement with full fat Hermetia illucens (HI) on the molecular mechanisms regulating lipid and bile salt (BA) homeostasis in rainbow trout (Oncorhynchus mykiss) juveniles. We thus explore the presence of 20-hydroxyecdysone (20E) in an insect meal-based diet and evaluate its potential involvement in regulating the molecular mechanisms/basis of FXR:RXR axis signaling. Ecdysteroids are a category of steroid hormones which bind a nuclear–receptor complex composed of ecdysone receptor (EcR) and ultraspiracle protein (USP) and regulate insect molting and metamorphosis. In all vertebrates, including fish, EcR-USP homologs are the Farnesoid X receptors (FXR) and the Retinoid X receptors (RXR), which are known to regulate crucial physiological and metabolic aspects, including BA synthesis and cholesterol homeostasis. In silico prediction indicates that 20E binds the heterodimeric complex with a binding affinity constant Kd equals to 610±60 nM and affects positively the dimerization process. Results also demonstrated the coordinated increased expression of FXR and RXR, as well as their downstream target genes (i.e. short heterodimer partner 1 and 2) in rainbow trout fed diets containing HI meal. This latter finding was paralleled by a significant down-regulation of CYP7a1 and CYP8b1 gene expression together with a decrease in hepatic total cholesterol, triglyceride, and BA levels. Overall, our study suggested that FXR is a potential target for 20E content in insect meal and provided preliminary data on the potential role of ecdysteroids in regulating the metabolic status of teleost fish through modulation of FXR signaling in the enterohepatic system.

Evidence of polybrominated diphenyl ethers (PBDEs) and alternative halogenated flame retardants (AHFRs) in wild fish species from the remote tropical marine environment, South China Sea

Polybrominated diphenyl ethers (PBDEs) and their alternatives (e.g., dechlorane plus (DPs) and decabromodiphenyl ethane (DBDPE)) are ubiquitous in various environmental media. However, limited data is available on these chemicals in edible fish species from the wide-open South China Sea (SCS). In the present study, ten legacy PBDEs and three substitutions (DBDPE and two DPs) were analyzed in 16 wild fish species sampled from the open SCS to investigate their spatial and species-specific variations. The results showed that the total concentrations of PBDEs, DBDPE, and DPs in fish samples were in the range of 1.69 to 47.6, not detected (nd) to 21.0, and nd to 3.80 ng/g lipid weight (lw), respectively. BDEs 47, 209 and 100 were the dominant target PBDE congeners, representing 49.2%, 17.2% and 9.93% of the total PBDE concentrations, respectively. Higher concentrations of PBDEs, DBDPE, and DPs were found in fish species from the Wanshan Archipelago compared to those from the Mischief Reef and the Yongxing Island, suggesting the significant influence of anthropogenic activities. Species-specific differences in levels of PBDEs were observed, with the order of bathydemersal > demersal > pelagic ≈ reef-associated > benthopelagic species. The average fanti value of all fish samples was 0.68, suggesting commercial DP products as a contamination source. The levels of PBDEs, DPs, and DBDPE in fish samples were relatively low compared with those from other locations around the globe. Finally, the health risks concerning the ingestion of BDEs 47, 99, 153 and 209 via fish consumption collected from the SCS are negligible.

Application of high-hydrostatic pressure to extend shelf life of miso-marinated Escolar loins during cold storage

The changes in the microbiological and chemical qualities of escolar meat following high-pressure processing (HPP) (300–600 MPa, 5 min) along with miso marinade for 15 days with refrigeration were evaluated. Miso marinade and HPP treatment of escolar meat had synergistic inactivating effects on microorganisms that were more pronounced at higher pressures. In addition, when stored at 4°C, the combination of miso marinade and HPP treatment significantly delayed microbial growth and total volatile basic nitrogen (TVBN) production and inhibited pH changes. Using TVBN < 25 mg/100 g as the criterion for freshness, miso marinade and HPP treatment (300–600 MPa) extended the refrigerated storage period from 3 days without treatment and 6 days with miso marinade alone to 15 days. In addition, high-throughput sequencing analysis showed that the combination of miso marinade and HPP treatment significantly altered the microbiome of the fish during refrigeration, thereby slowing the spoilage process. This study demonstrated that miso marinade along with HPP treatment can improve color and texture, inhibit microbial growth, delay quality deterioration, and prolong the shelf life of fish meat. Therefore, high pressure together with miso marinade is an effective hurdle technique for improving the quality and freshness of aquatic products.

Implementation of GMP and SSOP in Making Fish Chili Sauce

Chili Sauce is a famous food. Fish Chili Sauce made contains 10% fermented Tuna fish meat, to give a different taste. To get the quality of Fish Chili Sauce. The purpose of this study is to be able to apply the principles of Good Manufacturing Practice (GMP) and Sanitation Standard Operational Procedures (SSOP) in making fish Chili sauce. This study uses a Descriptive Method to see the Implementation of GMP and SSOP in Making Fish Chili Sauce. In each Principle of GMP and SSOP implementation, Objective and Subjective Microbial (TPC) and E.coli analysis will be carried out. The results of the study showed that the nutritional value of Fish Chili Sauce is Water 44.735%, Protein 4.595%, Fat 25.72%, Carbohydrate 4.1%, Ash 20.85%, pH 4.50 and TPC 2.4 x 10 4. With an Average Organoleptic Value of Taste 6.1 (Like), Aroma 6.5 (Like), Color 5.8 (Like), Texture and Overall Acceptance 6.2 (Like). The results of the Escherichia coli test from raw materials, equipment and also Fish Sambal showed Qualify.

Dietary full-fat or defatted black soldier fly larvae can replace protein sources with no detrimental effect on growth performance or intestinal health of nursery pigs.

This work aimed to determine the effects of dietary full-fat or defatted black soldier fly larvae (BSFL) to replace protein sources on growth performance, blood parameters, intestinal morphology, and intestinal microbiota in nursery pigs and to investigate the effects of dietary defatted BSFL at up to 30% at the expense of protein sources on growth performance in nursery pigs. In Exp. 1, a total of 36 barrows with an initial body weight of 7.0kg (standard deviation = 0.8) were allotted to 3 dietary treatments in a randomized complete block design with 4 replicate pens per treatment and 3 barrows per pen. A corn-soybean meal (SBM)-whey-based control diet was prepared with soy protein concentrate and fish meal as additional protein supplements. Two additional diets were prepared to include 20% full-fat BSFL or 20% defatted BSFL to replace soy protein concentrate and fish meal to maintain the same energy and nutrient concentrations in all diets. In the 28-day feeding trial, pigs fed the diet containing defatted BSFL tended to consume more feeds (P < 0.10) than other groups during days 14 to 28 and the overall period. On day 28, the serum blood urea nitrogen in pigs fed the control diet was less (P < 0.05) than that fed the full-fat or defatted BSFL, but fecal score and jejunal morphology did not differ among the treatment groups. Relative abundance of Mycoplasma in the ileal digesta was less (P < 0.05) in the pigs fed the diet containing full-fat or defatted BSFL compared with the control group. In Exp. 2, a total of 192 pigs with an initial body weight of 7.8 (standard deviation = 1.2kg) were randomly allotted to one of 4 dietary treatments in a randomized complete block design with 6 replicate pens per treatment and 4 barrows and 4 gilts per pen. A control diet was mainly based on corn, SBM, fermented SBM, fish meal, and spray-dried plasma protein. Three additional diets were prepared to contain 10%, 20%, and 30% defatted BSFL to replace SBM, fermented SBM, fish meal, and spray-dried plasma protein to maintain for the same energy and nutrient concentrations. Average daily gain, average daily feed intake, gain:feed, and fecal score were not affected by increasing dietary defatted BSFL. Overall, dietary black soldier fly larvae did not compromise growth performance or intestinal health in nursery pigs. Black soldier fly larvae can be used in nursery pig diets to replace other protein sources without negative effects.

Bioconversion of insect protein biomass into a fishmeal alternate for freshwater fish, Oreochromis mossambicus (Peters, 1852) diets

Abstract The demand for alternative fishmeal has increased significantly in recent years due to the expansion of the fishing industry. Therefore, insect proteins are becoming more popular as an alternative low-cost bio-source for fish than plant-based fish feeds. In the present study, a dietary mixture of insect protein powder derived from four Indian grasshopper species, namely Oxya hyla hyla, Atractomorpha crenulata, Oxya japonica japonica, and Phlaeoba infumata (insect meal, IM) was substituted for conventionally used fishmeal (FM) in the diet formulation for growing Mozambique tilapia, Oreochromis mossambicus (Peters, 1852) fingerlings. Three formulated diets with IM were prepared, with FM substituted at the rates of 25%, 50%, and 75%, respectively, with a control diet with no IM but only 20% FM. All dietary treatments were administered to tilapia fingerlings for 60 days under laboratory conditions. IM-incorporated diets outperform FM-incorporated diets in growth and digestibility due to their higher food conversion ratio for IM. In terms of growth and survivability in tilapia fingerlings, a 75% replacement of FM with IM demonstrated the most significant efficacy. According to the findings, mixed insect powder derived from four grasshopper species can be used as a partial replacement for FM in formulating the compound fish diet for tilapia fingerlings.

The effect of experimental diets incorporating fermented soybean meal on growth metrics and utilization of nutrients in broiler chickens

The aim of this study was to explore the impact of fermented soybean meal (FSBM) on the nutritional quality of feed, growth performance indicators, and nutrient utilization in broiler chickens, aiming to overcome the challenges associated with locally produced soybean meal stemming from anti-nutrients and variations in nutritional quality. The study methodology included assessing growth performance metrics through two-way ANOVA statistical analysis and evaluating nutrient utilization in broiler chickens. Results indicated that broiler chickens fed with FSBM exhibited superior performance, displaying the highest average weekly weight gain (452 grams), optimal feed conversion ratio (1.39), extended intestinal length (225 cm), increased intestinal weight (297.5 grams), and heightened activity of digestive enzymes compared to other treatment groups throughout the feeding trials. The results of this study suggest that FSBM’s better digestibility helps broiler chickens grow faster. This makes it a possible replacement for animal protein sources like fish meal in poultry feed production, which is good for both local poultry feed producers and consumers.

Bovine bone meal combined with dephenolized cottonseed protein is an effective fishmeal substitution in Trachinotus ovatus feed: A comprehensive evaluation of growth, muscle quality, and intestinal digestion physiology

To investigate the feasibility of substituting fishmeal (FM) with a combination of bovine bone meal (BBM) and dephenolized cottonseed protein (DCSP), a mixture (consisting of 60 % BBM and 40 % DCSP) was used to replace varying FM levels in Golden pompano (Trachinotus ovatus) feed. Five isoproteic (47 %) and isolipidic (12 %) experimental diets were formulated, with FM levels set at 30 %, 24 %, 18 %, 12 %, and 6 % (namely Control, FM24, FM18, FM12, and FM6). To compensate for the reduced FM content, BBM and DCSP were used as substitutes, and DL-methionine and L-lysine were added to maintain the dietary amino acid balance. Following a 63-day trial, results showed that the growth of FM24, FM18 and FM12 groups did not exhibit significant differences compared to the Control group, while those of FM6 group considerably decreased. The biochemical composition of whole fish did not display significant differences among the five groups, while the muscle protein contents of the FM12 group were higher than the Control group. Most of the muscle texture properties were positively modified in the FM 12 and FM 6 groups. With the substitution ratio increase, the contents of serum total protein, albumin, total amino acids, and cholecystokinin were elevated, while serum peptide YY contents decreased. In addition, intestinal trypsin, NaK-ATPase, and alkaline phosphatase activities in FM18, FM12, and FM6 groups decreased, and lipase activities exhibited the opposite trend. The transcription levels of genes related to amino acid-sensing receptors (asct2 and snat2) were down-regulated with increasing dietary FM substitutions, while the expression levels of appetite regulation factors (pept1, cck, and ghrl) were up-regulated. In conclusion, the FM content in the diet of T. ovatus can be reduced to 12 % through the utilization of a combination of BBM and DCSP, with no adverse effects observed on growth performance, biochemical composition, or muscle texture.