Eastern Coyotes Research Articles

Ecosystem service assessment of coyote stories reveals tradeoffs from human–coyote interactions in rural Vermont, United States

AbstractThe ecosystem system services framework has potential to help clarify wildlife management challenges at the single species scale, but existing methods struggle to capture the complex values and tradeoffs at play in human–wildlife interactions. We worked with community scientists to gather and use stories (n = 150) as a source of ecosystem services data about living alongside eastern coyotes (Canis latrans var) in rural Vermont, United States. Our a priori ecosystem service assessment showed that human–coyote interactions can have simultaneous positive and negative human well‐being impacts at both the sample scale and for individual interviewees. Our research identified emergent themes that lent insight into how interviewees justified different kinds of relationships with coyotes. We applied a tradeoffs lens to evaluate three policy options based on their potential to mitigate ecosystem disservices and maximize ecosystem services from human–coyote interactions. We found that ecosystem services assessment rooted in a dataset of stories revealed policy‐relevant understanding of value conflicts and alignments at the sample scale without overshadowing the nuances of individual interviewees' experiences.

Borreliella burgdorferi factor H-binding proteins are not required for serum resistance and infection in mammals.

The causative agent of Lyme disease (LD), Borreliella burgdorferi, binds factor H (FH) and other complement regulatory proteins to its surface. B. burgdorferi B31 (type strain) encodes five FH-binding proteins (FHBPs): CspZ, CspA, and the OspE paralogs OspEBBN38, OspEBBL39, and OspEBBP38. This study assessed potential correlations between the production of individual FHBPs, FH-binding ability, and serum resistance using a panel of infectious B. burgdorferi clonal populations recovered from dogs. FHBP production was assessed in cultivated spirochetes and by antibody responses in naturally infected humans, dogs, and eastern coyotes (wild canids). FH binding specificity and sensitivity to dog and human serum were also assessed and compared. No correlation was observed between the production of individual FHBPs and FH binding with serum resistance, and CspA was determined to not be produced in animals. Notably, one or more clones isolated from dogs lacked CspZ or the OspE proteins (a finding confirmed by genome sequence determination) and did not bind FH derived from canines. The data presented do not support a correlation between FH binding and the production of individual FHBPs with serum resistance and infectivity. In addition, the limited number and polymorphic nature of cp32s in B. burgdorferi clone DRI85A that were identified through genome sequencing suggest no strict requirement for a defined set of these replicons for infectivity. This study reveals that the immune evasion mechanisms employed by B. burgdorferi are diverse, complex, and yet to be fully defined.

Humans drive spatial variation in mortality risk for a threatened wolf population in a Canis hybrid zone

Abstract Large carnivores often exhibit high survival rates in protected areas, whereas intentional and unintentional human‐caused mortality may be greater in adjacent areas. These patterns can result in source‐sink dynamics and limit population expansion beyond protected areas. We used telemetry data from 438 canids in 141 packs collected from 2002 to 2020 to evaluate mortality risk for wolves, coyotes, and admixed canids in a 3‐species hybrid zone in and adjacent to a large protected area in Ontario, Canada. The hybrid zone is occupied by most of the remaining eastern wolves (Canis lycaon), a rare, threatened species that hybridizes with sympatric eastern coyotes (C. latrans) and Great Lakes grey wolves (C. lupus). Within Algonquin Provincial Park (APP), annual human‐caused mortality from harvest and vehicles was low (0.06, 95% CI [0.03, 0.08]), whereas annual human‐caused mortality was higher in adjacent areas (0.31, 95% CI [0.25, 0.37]). Smaller protected areas implemented to help protect eastern wolves did not significantly reduce mortality. Eastern wolves survived poorly relative to other canids and dispersing canids survived poorly relative to residents. Mortality risk was greater when canids were closer to roads. Mortality risk was also increased or reduced by the strength of individual‐level selection or avoidance of roads relative to their availability, respectively. Our results provide a comprehensive evaluation of factors influencing spatial variation in mortality risk for canids to inform eastern wolf recovery efforts. Additionally, we developed a novel modelling approach for investigating the influence of resource selection on mortality risk, which highlighted that individual‐level responses to risk can strongly influence population‐level mortality patterns. Synthesis and applications. Despite being listed as ‘threatened’ under the Ontario Endangered Species Act, eastern wolves are still legally trapped and shot outside protected areas in central Ontario. Eastern wolves and dispersing canids survive poorly outside of APP, primarily from human‐caused mortality. These results, along with the apparent inadequacy of the smaller protected areas, suggest that expanding the threatened eastern wolf population outside APP is unlikely under current management conditions. Protecting eastern wolves from human‐caused mortality is complicated as it would require a harvest ban for all canids, including coyotes.

Tracing Eastern Wolf Origins From Whole-Genome Data in Context of Extensive Hybridization.

Southeastern Canada is inhabited by an amalgam of hybridizing wolf-like canids, raising fundamental questions regarding their taxonomy, origins, and timing of hybridization events. Eastern wolves (Canis lycaon), specifically, have been the subject of significant controversy, being viewed as either a distinct taxonomic entity of conservation concern or a recent hybrid of coyotes (C. latrans) and grey wolves (C. lupus). Mitochondrial DNA analyses show some evidence of eastern wolves being North American evolved canids. In contrast, nuclear genome studies indicate eastern wolves are best described as a hybrid entity, but with unclear timing of hybridization events. To test hypotheses related to these competing findings we sequenced whole genomes of 25 individuals, representative of extant Canadian wolf-like canid types of known origin and levels of contemporary hybridization. Here we present data describing eastern wolves as a distinct taxonomic entity that evolved separately from grey wolves for the past ∼67,000 years with an admixture event with coyotes ∼37,000 years ago. We show that Great Lakes wolves originated as a product of admixture between grey wolves and eastern wolves after the last glaciation (∼8,000 years ago) while eastern coyotes originated as a product of admixture between "western" coyotes and eastern wolves during the last century. Eastern wolf nuclear genomes appear shaped by historical and contemporary gene flow with grey wolves and coyotes, yet evolutionary uniqueness remains among eastern wolves currently inhabiting a restricted range in southeastern Canada.

Toward a More-Than-Human Everyday Urbanism: Rhythms and Sensoria in the Multispecies City

This article advances a more-than-human everyday urbanism as a useful analytic for articulating a less anthropocentric reading of the city. Using a case study of eastern coyotes (Canis latrans) in the Greater Toronto Area, Canada, it draws on empirical data and ethological literatures to consider everyday life in multispecies cities through two registers: rhythms and sensoria. It first performs an experimental rhythmanalysis, demonstrating the import of linear and cyclical rhythms in the space-times of human–coyote encounters. It then delves into coyote sensory worlds, illustrating the acoustic and olfactory ecologies that shape urban atmospheres and place-making. The article argues that a more-than-human everyday urbanism holds value for practice and politics, shifting the focus from spectacular moments of conflict with wildlife to a consideration of more-than-human place making, resituating urban animals as neighbors rather than invaders. This analysis contributes to emergent conversations in more-than-human and urban geographies aimed at making visible other-than-human spaces, practices, and experiences, a project central to recuperating the urban as an existing and potential site of multispecies flourishing.

The natural history and ecology of melanism in red wolf and coyote populations of the southeastern United States – evidence for Gloger’s rule

BackgroundGloger’s rule postulates that animals should be darker colored in warm and humid regions where dense vegetation and dark environments are common. Although rare in Canis populations, melanism in wolves is more common in North America than other regions globally and is believed to follow Gloger’s rule. In the temperate forests of the southeastern United States, historical records of red wolf (Canis rufus) and coyote (Canis latrans) populations document a consistent presence of melanism. Today, the melanistic phenotype is extinct in red wolves while occurring in coyotes and red wolf-coyote hybrids who occupy the red wolf's historical range. To assess if Gloger’s rule could explain the occurrence and maintenance of melanistic phenotypes in Canis taxa, we investigated differences in morphology, habitat selection, and survival associated with pelage color using body measurements, GPS tracking data, and long-term capture-mark-recapture and radio-telemetry data collected on coyotes and hybrids across the southeastern United States.ResultsWe found no correlation between morphometrics and pelage color for Canis taxa. However, we observed that melanistic coyotes and hybrids experienced greater annual survival than did their gray conspecifics. Furthermore, we observed that melanistic coyotes maintained larger home ranges and exhibited greater selection for areas with dense canopy cover and wetlands than did gray coyotes.ConclusionsIn the southeastern United States, pelage color influenced habitat selection by coyotes and annual survival of coyotes and hybrids providing evidence that Gloger’s rule is applicable to canids inhabiting regions with dense canopy cover and wetlands. Greater annual survival rates observed in melanistic Canis may be attributed to better concealment in areas with dense canopy cover such as coastal bottomland forests. We suggest that the larger home range sizes of melanistic coyotes may reflect the trade-off of reduced foraging efficiency in lower quality wetland habitat for improved survival. Larger home ranges and differential use of land cover by melanistic coyotes may facilitate weak assortative mating in eastern coyote populations, in which melanistic animals may have lower success of finding compatible mates in comparison to gray conspecifics. We offer that our observations provide a partial explanation for why melanism is relatively low (< 10%) but consistent within coyote populations throughout southeastern parts of their range.

The hybrid skull of the eastern coyote (Canis latrans var.): Nonmetric traits and craniomandibular shape

The increasing awareness that hybridization, and resultant gene flow, plays a major role in animal diversification has led to a growing number of studies that have focused on assessing the morphological consequences of this process. Analyses of mammalian hybrids have identified skeletal effects of hybridization, including a suite of anomalous dental and sutural traits on the skull that are present at high frequencies in hybrid populations. These studies have also detected consistent patterns of morphological shape and size differences between hybrids and parental taxa across a wide variety of organisms. However, more research is required to understand the universality of these traits and shape/size differences. Building on these previous studies, a sample of genetically determined canid hybrids was examined, specifically the eastern coyote (Canis latrans var.), a hybrid between coyotes, wolves, and dogs, to test whether this group exhibits a comparable pattern of anomalous nonmetric characters, and to assess differences in craniomandibular shape and size. First, specimens of C. latrans var., C. latrans, and C. lupus were scored for anomalous traits, including supernumerary and rotated teeth, dental crowding, and sutural anomalies. Geometric morphometric analyses were then conducted on a subset of these individuals to explore craniomandibular size and shape variation, as well as allometry. The results are largely consistent with other studies, indicating that the incidence of dental anomalies, dental crowding, and sutural anomalies is significantly higher in hybrids. However, differences are not significant for supernumerary teeth. The exploration of morphometric variation identifies intermediate morphology in the hybrids, and some indication of greater morphological variability in the mandible. When these results are combined with previous studies, they suggest that skeletal signatures of hybridization are common to different mammalian taxa across multiple generations; however, some traits such as supernumerary teeth may be lost after a few generations.

Considering Pleistocene North American wolves and coyotes in the eastern Canis origin story.

The evolutionary origins and hybridization patterns of Canis species in North America have been hotly debated for the past 30 years. Disentangling ancestry and timing of hybridization in Great Lakes wolves, eastern Canadian wolves, red wolves, and eastern coyotes are most often partitioned into a 2‐species model that assigns all ancestry to gray wolves and/or coyotes, and a 3‐species model that includes a third, North American evolved eastern wolf genome. The proposed models address recent or sometimes late Holocene hybridization events but have largely ignored potential Pleistocene era progenitors and opportunities for hybridization that may have impacted the current mixed genomes in eastern Canada and the United States. Here, we re‐analyze contemporary and ancient mitochondrial DNA genomes with Bayesian phylogenetic analyses to more accurately estimate divergence dates among lineages. We combine that with a review of the literature on Late Pleistocene Canis distributions to: (a) identify potential Pleistocene progenitors to southern North American gray wolves and eastern wolves; and (b) illuminate opportunities for ancient hybridization events. Specifically, we propose that Beringian gray wolves (C. lupus) and extinct large wolf‐like coyotes (C. latrans orcutti) are likely progenitors to Mexican and Plains gray wolves and eastern wolves, respectively, and may represent a potentially unrecognized source of introgressed genomic variation within contemporary Canis genomes. These events speak to the potential origins of contemporary genomes and provide a new perspective on Canis ancestry, but do not negate current conservation priorities of dwindling wolf populations with unique genomic signatures and key ecologically critical roles.

Detection of Borrelia spp., Ehrlichia canis, Anaplasma phagocytophilum, and Dirofilaria immitis in Eastern Coyotes (Canis latrans) in Nova Scotia, Canada.

Borrelia burgdorferi and Borrelia miyamotoi are tickborne zoonotic pathogens in Canada. Both bacteria are vectored by ticks, Ixodes scapularis in Atlantic Canada, but require wildlife reservoir species to maintain the bacteria for retransmission to future generations of ticks. Coyotes (Canis latrans) are opportunistic feeders, resulting in frequent contact with other animals and with ticks. Because coyotes are closely related to domestic dogs (Canis lupus familiaris), it is probable that coyote susceptibility to Borrelia infection is similar to that of dogs. We collected livers and kidneys of eastern coyotes from licensed harvesters in Nova Scotia, Canada, and tested them using nested PCR for the presence of B. burgdorferi, B. miyamotoi, and Dirofilaria immitis. Blood obtained from coyote livers was also tested serologically for antibodies to B. burgdorferi, Ehrlichia canis, Anaplasma phagocytophilum, and D. immitis. Borrelia burgdorferi and D. immitis were detected by both nested PCR and serology tests. Seroreactivity to A. phagocytophilum was also found. Borrelia miyamotoi and E. canis were not detected. Our results show that coyotes in Nova Scotia have been exposed to a number of vectorborne pathogens.

Demographical and morphological differences among coyotes (Canis latrans) relative to sampling method

Collection methods can be biased, leading to misperceptions of population composition. We tested if collection method (footholds, snares, and shooting) gave different perceptions of demography or morphology of 3539 eastern coyotes (Canis latrans Say, 1823). We found no differences in sex ratios of animals among methods, but did find some evidence that younger, lighter, and smaller animals were more likely to be collected by footholds than with snares. Female reproductive histories (placental scars) did not differ among methods. In a subsample of 232 animals, we found no evidence of differences in helminth parasitism relative to collection method. Overall, our large sample for the non-parasite analyses facilitated finding statistical significance; the biological implications hinge on the precision required in estimating population composition and the focal characteristics being compared. For example, mass was 5.3% lower for coyotes caught with footholds versus snares and 10.4% lower for coyotes caught with footholds versus being shot, whereas linear trait measurements of coyotes caught with footholds were generally smaller by at most 4.5% compared with other methods (broadly consistent with linear versus volumetric measurements). Our study provides important baseline information for making inferences about populations of coyotes (and other species) sampled using only a single collection method.

Resource selection at homesites by wolves and eastern coyotes in a Canis hybrid zone

AbstractWe modeled resource selection by wolves (Canis spp.), eastern coyotes (C. latrans), and admixed canids during the pup‐rearing season at den and rendezvous sites (collectively, homesites) within a largely unprotected landscape proposed as the recovery zone for federally and provincially threatened eastern wolves (C. lycaon) in Ontario, Canada. Overall, canids selected wetlands, while avoiding secondary roads and open‐structure rock‐grass habitat patches. Packs with greater wolf ancestry selected wetlands and tertiary roads more strongly, while avoiding mixed conifer‐hardwood forests. Contrary to our prediction, canids with greater coyote ancestry did not establish homesites closer to roads, which likely mitigated their risk of human‐caused mortality during pup‐rearing. Packs exhibited increased selection of wetlands within territories as a function of increasing availability of wetlands. Packs with abundant access to wetlands may prioritize this habitat type to exploit beavers, a valuable prey species during pup‐rearing. Packs with higher pup survival selected hardwood forests and avoided conifer forests more than packs with lower pup survival. This is consistent with our understanding of habitat relations of the main prey species for canids in central Ontario and suggests that selecting prey‐rich habitat types at homesites increases fitness. A proposed goal of eastern wolf recovery is numerical and geographical expansion outside of the population core in Algonquin Provincial Park. Thus, our results provide valuable information for conservation by quantifying resource selection of wolves, coyotes, and hybrids during pup‐rearing and identifying links between fitness and homesite selection.

From diet to hair and blood: empirical estimation of discrimination factors for C and N stable isotopes in five terrestrial mammals

AbstractCarbon and nitrogen stable isotope ratios are used widely to describe wildlife animal diet composition and trophic interactions. To reconstruct consumer diet, the isotopic differences between consumers and their diet items—called the trophic discrimination factor (TDF)—must be known. Proxies of diet composition are sensitive to the accuracy of TDFs. However, specific TDFs are still missing for many species and tissues because only a few controlled studies have been carried out on captive animals. The aim of this study was to estimate TDFs for hair and blood for carbon and nitrogen stable isotopes for caribou, moose, white-tailed deer, eastern coyote, and black bear. We obtained stable isotope ratios for diet items, hair, and blood samples, of 21 captive adult mammals. Diet–tissue discrimination factors for carbon in hair (∆ 13CLE) ranged from 0.96‰ to 3.72‰ for cervids, 3.01‰ to 3.76‰ for coyote, and 5.15‰ to 6.35‰ for black bear, while nitrogen discrimination factors (∆ 15N) ranged from 2.58‰ to 5.95‰ for cervids, 2.90‰ to 3.13‰ for coyote, and 4.48‰ to 5.44‰ for black bear. The ∆ 13CLE values in coyote blood components ranged from 2.20‰ to 2.69‰ while ∆ 15N ranged from 3.30‰ to 4.41‰. In caribou serum, ∆ 13CLE reached 3.34 ± 1.28‰ while ∆ 15N reached 5.02 ± 0.07‰. The TDFs calculated in this study will allow the evaluation of diet composition and trophic relationships between these five mammal species and will have important implications for the study of endangered caribou populations for which the use of noninvasive tissue sampling is highly relevant.

Serologic Evidence for the Exposure of Eastern Coyotes ( Canis latrans ) in Pennsylvania to the Tick-Borne Pathogens Borreliella burgdorferi and Anaplasma phagocytophilum

Lyme disease and anaplasmosis are tick-borne bacterial diseases caused by Borreliella and Anaplasma species, respectively. A comprehensive analysis of the exposure of eastern coyotes (Canis latrans) in the northeastern United States to tick-borne pathogens has not been conducted. In this report, we assess the serological status of 128 eastern coyotes harvested in Pennsylvania in 2015 and 2017 for antibodies to Borreliella burgdorferi and Anaplasma phagocytophilum Immunoblot and dot blot approaches were employed to test each plasma sample by using cell lysates and recombinant proteins as detection antigens. The results demonstrate high seropositivity incidences of 64.8% and 72.7% for B. burgdorferi and A. phagocytophilum, respectively. Antibodies to both pathogens were detected in 51.5% of the plasma samples, indicating high potential for coinfection. Antibodies to the B. burgdorferi proteins DbpB, VlsE, DbpA, BBA36, and OspF (BBO39) were detected in 67.2, 63.3, 56.2, 51.6, and 48.4% of the plasma samples, respectively. Antibodies to the A. phagocytophilum P44 and P130 proteins were detected in 72.7 and 60.9% of the plasma samples, respectively.IMPORTANCE The incidence of Lyme disease (Borreliella burgdorferi) and anaplasmosis (Anaplasma phagocytophilum) are increasing in North America and Europe. The causative agents of these debilitating tick-transmitted infections are maintained in nature in an enzootic cycle involving Ixodes ticks and diverse mammals and birds. It has been postulated that predators directly or indirectly influence the dynamics of the enzootic cycle and disease incidence. Here, we demonstrate high seropositivity of eastern coyotes for B. burgdorferi and A. phagocytophilum As coyotes become established in urban and suburban environments, interactions with humans, companion animals, and urban/suburban wildlife will increase. Knowledge of the pathogens that these highly adaptable predators are exposed to or carry, and their potential to influence or participate in enzootic cycles, is central to efforts to reduce the risk of tick-borne diseases in humans and companion animals.

Eastern Coyotes (&lt;i&gt;Canis latrans&lt;/i&gt; var.) consuming large ungulates in a multi-ungulate system

The feeding habits of generalist predators often vary among populations and regions. For example, Coyote (Canis latrans), which is a generalist predator distributed across North America, occupies a wide range of habitats and has a highly varied diet. In this observational study, we quantified the presence of mammalian prey items in 50 Eastern Coyote (Canis latrans var.) scats collected in late spring and summer in a private game reserve in southwestern Quebec. Nearly all scats contained hair of White-tailed Deer (Odocoileus virginianus; 44%), Moose (Alces americanus; 38%), or American Beaver (Castor canadensis; 38%). Although all three species are known to be consumed by coyotes, such a high proportion of Moose and White-tailed Deer simultaneously occurring in the diet of coyotes has not been previously reported. The uniqueness of the study area, with its relatively high abundance of all three prey species, may account for the uniqueness of the diet of Eastern Coyotes living there.

High reliance on a diet of Moose (&lt;i&gt;Alces americanus&lt;/i&gt;) by Eastern Coyotes (&lt;i&gt;Canis latrans&lt;/i&gt; var.) in Cape Breton Highlands National Park, Nova Scotia, Canada

Eastern Coyote (Canis latrans var.) scats were collected along transects in Cape Breton Highlands National Park, Nova Scotia, Canada, from May 2012 to August 2013 to determine diet. Based on 294 scats, Moose (Alces americanus) remains made up the highest percentage by volume in scats during fall, winter, and spring. During the summer, Moose remains were found in over 30% of scats (18% by volume), although fruit and berries were more commonly found. No other study has documented such high annual use of Moose. As there was no evidence that the consumed Moose were killed by Coyotes, presumably Coyotes scavenged Moose that had died of natural causes.

Genetic diversity and relatedness of a recently established population of eastern coyotes (Canis latrans) in New York City

Little is known about the relatedness structure of carnivores living in urban areas, where green spaces may vary in size and resource availability. We examined the minimum population size, relatedness structure, and genetic diversity of a recently established population of eastern coyotes (Canis latrans) inhabiting New York City (NYC). The population has been established for approximately 25 years, and sample collection for genetic analysis has been ongoing since 2010. We genotyped 234 scat, eight tissue, and three blood samples at nine microsatellite loci. We identified 45 individual coyotes with a male-biased sex ratio of 2.2:1. We also found moderate to high levels of genetic diversity, with average observed heterozygosity of 0.779 and mean number of alleles per locus of 7.8. Most of the green spaces surveyed supported a single group of closely related coyotes in each. Relatedness comparisons between parks also indicated that coyotes compared across different parks were also closely related. We identified two unrelated mated pairs and found no support for polygamy. The high incidence of relatedness suggests that the coyote population is descended from a small number of founding individuals. Additionally, we genetically recaptured several coyotes, including one individual sampled in the Bronx and in Queens, with a median of 103 days between resampling. This result indicates that the coyotes are persisting in some of the isolated greenspaces of New York City and able to move successfully between them.

Effects on white‐tailed deer following eastern coyote colonization

ABSTRACTThe expansion or recovery of predators can affect local prey populations. Since the 1940s, coyotes (Canis latrans) have expanded into eastern North America where they are now the largest predator and prey on white‐tailed deer (Odocoileus virginianus). However, their effect on deer populations remains controversial. We tested the hypothesis that coyotes, as a novel predator, would affect deer population dynamics across large spatial scales, and the strongest effects would occur after a time lag following initial coyote colonization that allows for the predator populations to grow. We evaluated deer population trends from 1981 to 2014 in 384 counties of 6 eastern states in the United States with linear mixed models. We included deer harvest data as a proxy for deer relative abundance, years since coyote arrival in a county as a proxy of coyote abundance, and landscape and climate covariates to account for environmental effects. Overall, deer populations in all states experienced positive population growth following coyote arrival. Time since coyote arrival was not a significant predictor in any deer population models and our results indicate that coyotes are not controlling deer populations at a large spatial scale in eastern North America. © 2019 The Wildlife Society.

Geographic patterns in morphometric and genetic variation for coyote populations with emphasis on southeastern coyotes.

Prior to 1900, coyotes (Canis latrans) were restricted to the western and central regions of North America, but by the early 2000s, coyotes became ubiquitous throughout the eastern United States. Information regarding morphological and genetic structure of coyote populations in the southeastern United States is limited, and where data exist, they are rarely compared to those from other regions of North America. We assessed geographic patterns in morphology and genetics of coyotes with special consideration of coyotes in the southeastern United States. Mean body mass of coyote populations increased along a west‐to‐east gradient, with southeastern coyotes being intermediate to western and northeastern coyotes. Similarly, principal component analysis of body mass and linear body measurements suggested that southeastern coyotes were intermediate to western and northeastern coyotes in body size but exhibited shorter tails and ears from other populations. Genetic analyses indicated that southeastern coyotes represented a distinct genetic cluster that differentiated strongly from western and northeastern coyotes. We postulate that southeastern coyotes experienced lower immigration from western populations than did northeastern coyotes, and over time, genetically diverged from both western and northeastern populations. Coyotes colonizing eastern North America experienced different selective pressures than did stable populations in the core range, and we offer that the larger body size of eastern coyotes reflects an adaptation that improved dispersal capabilities of individuals in the expanding range.

High genomic diversity and candidate genes under selection associated with range expansion in eastern coyote (Canis latrans) populations.

Range expansion is a widespread biological process, with well‐described theoretical expectations associated with the colonization of novel ranges. However, comparatively few empirical studies address the genomic outcomes accompanying the genome‐wide consequences associated with the range expansion process, particularly in recent or ongoing expansions. Here, we assess two recent and distinct eastward expansion fronts of a highly mobile carnivore, the coyote (Canis latrans), to investigate patterns of genomic diversity and identify variants that may have been under selection during range expansion. Using a restriction‐associated DNA sequencing (RADseq), we genotyped 394 coyotes at 22,935 SNPs and found that overall population structure corresponded to their 19th century historical range and two distinct populations that expanded during the 20th century. Counter to theoretical expectations for populations to bottleneck during range expansions, we observed minimal evidence for decreased genomic diversity across coyotes sampled along either expansion front, which is likely due to hybridization with other Canis species. Furthermore, we identified 12 SNPs, located either within genes or putative regulatory regions, that were consistently associated with range expansion. Of these 12 genes, three (CACNA1C, ALK, and EPHA6) have putative functions related to dispersal, including habituation to novel environments and spatial learning, consistent with the expectations for traits under selection during range expansion. Although coyote colonization of eastern North America is well‐publicized, this study provides novel insights by identifying genes associated with dispersal capabilities in coyotes on the two eastern expansion fronts.

Initial colonization of Long Island, New York by the eastern coyote,&amp;nbsp;Canis latrans (Carnivora, Canidae), including first record of breeding

Coyotes (Canis latrans Say, 1823) have increased their range dramatically over the past century. Formerly restricted&nbsp;to western North America, they now roam across the continent, in many habitats including large cities. One of the last&nbsp;areas in North America without coyotes has been Long Island, NY, a 3629 km2 island in the New York metropolitan&nbsp;area. Here&nbsp; we summarize all verified accounts of coyotes on Long Island, including the first record of breeding. There&nbsp;are few coyotes on Long Island currently; however, given the history of coyote success, we expect coyotes to establish&nbsp;a growing population there in the near future.