The improvement of sweet sorghum (Sorghum bicolor) for biofuel traits is getting more attention globally due to its sugar-rich stalk that can be used as a renewable energy product. An understanding and proper assessment of biofuel-related traits in sweet sorghum crop is an important step toward the development of superior cultivar. The study was conducted to characterize sweet sorghum collections of Ethiopia for 0Brix degree, some morphological and grain yield traits. A total of 180 accessions were collected and evaluated in two environments. The result revealed wide variability among the accessions collected from different regions. Collections from northern part of Ethiopia (Wello and Tigray) showed significantly high 0Brix mean value while collections from the rest of the regions (Hararge, West Shewa, East Wollega and Gojam) expressed lower 0Brix mean value but higher biomass for stalk sugar yield. Most of the accessions showed higher 0Brix mean value, which is similar to globally known sweet sorghum cultivars. Accessions with high 0Brix degree combined with high biomass traits like stalk diameter and plant height will be an ideal germplasm to be used for biofuel production. Hence, it is imperative to utilize these accessions in sweet sorghum breeding program in order to develop superior sugar-rich sweet sorghum cultivars.