Background: This study aims to identify risk factors for Rhipicephalus (Boophilus) microplus ticks’ infestation in South Gujarat’s cattle and explore its implications on Anaplasma marginale. Methods: An approach was employed to retrieve the crucial insights from the owners and examinations of 2305 cattle (crossbred=1495, indigenous=810) of South Gujarat, India. Species specific PCR was used to detect 576 bp of msp5 gene of A. marginale in the oviposited ticks. Result: Crossbred cattle (73.91%) had higher tick prevalence than indigenous ones (55.80%), with R. (B.) microplus being the dominant species. Highest tick infestation was noted in cattle greater than 3 years (74.43%), followed by less than 1 year (70.23%) and 1-3 years old (55.60%). Ticks collected from crossbred exhibited higher prevalence of A. marginale compared to indigenous cattle. Significantly highest (p greater than 0.05) ticks infestation rate was noted in cattle with pale conjunctiva, poor body condition and black coat colour. Infection rate of both the parasites was highest during summer, followed by monsoon and winter. Kachcha house and earthen floor favoured the survival of the pathogen. Owners in surveyed regions combat tick infestations using mechanical (7.69%), chemical (83.51%) and herbal/no treatments (10.07%). These findings emphasize the intricate link between tick infestations and A. marginale infection, offering crucial insights for cattle health management.