Earth Ponds Research Articles

Doxycycline pharmacokinetics and tissue depletion in striped catfish (Pagasianodon hypophthalmus) after oral administration.

The pharmacokinetics and residue depletion of doxycycline (DOX) in striped catfish (Pagasianodon hypophthalmus) after oral dosage were investigated. The pharmacokinetic experiment was conducted in an aquarium, while the experiment of residue depletion was performed in both an aquarium and earth ponds. Medicated feed was administered orally using the gavage method at a dosage of 20 mg/kg body weight. Blood, liver, and kidney from medicated fish samples were collected. In the depletion experiments, fish were fed medicated feed for five consecutive days at a dosage of 20 mg/kg body weight, with samples collected during and after medication. The concentrations of DOX were quantified using an LC-MS/MS system. The pharmacokinetics parameters of DOX in striped catfish included the absorption rate constant (ka), absorption half-life (T1/2abs), maximal plasma concentration (Cmax), time to maximal plasma concentration (Tmax), and area under the plasma concentration-time curve from time 0 to 96 h (AUC0-96 h) which were 0.12 h-1, 5.68 h, 1123.45 ng/mL, 8.19 h, and 25,018 ng/mL/h, respectively. Residue depletion results indicated that the withdrawal times of DOX in muscle (with skin) from fish kept in the aquarium were slightly longer than that in fish raised in earth ponds, corresponding to 194 degree-days compared with 150 degree-days. In conclusion, administration of DOX at the dosage of 20 mg/kg body weight can be used for treatment of bacterial infections in striped catfish, and a withdrawal time of 5 days at 29.4°C will ensure consumer food safety due to the rapid depletion of DOX from muscle and skin.

Blue crabs (Callinectes sapidus Rathbun, 1896): Initial Adaptation Studies (Brachyura: Portunidae)

The purpose of this study was to determine the blue crab's growth parameters and explore potential adaptations while it was kept in an earthen pond for ninety days. After samples from October 2012 and August 2013 were analyzed, the breeding and biological characteristics of the blue crab (Callinectes sapidus Rathbun, 1896) in the South Aegean were determined. Blue crabs' adaptation to the earth pond, growth circumstances, and morphometric measuremalets using information from the literature are discussed. The process of adaptation took a week, during which time individual blue crabs were seen to begin feeding. The Ege the University Faculty received nine units from the Pond in the Kusadasi/Güzelçamlı area that were examined in cages with 108 distinct experimaletal groups of persons out of 20 living humales.

Growth of Gracilaria sp. in Monoculture and Polyculture System with Milkfish (Chanos chanos Forsk) in Traditional Ponds, Brebes Regency, Central Java

Gracilaria sp. has a high economic value, which can be cultivated in monoculture or polyculture with other cultivars, such as milkfish (Chanos chanos Forsk). Polyculture of Gracilaria sp. with milkfish can increase the growth of Gracilaria sp. because the metabolic products of milkfish are helpful as a source of nutrients for Gracilaria sp. This study aimed to (1) examine the effect of Gracilaria sp. cultivation in monoculture and polyculture with milkfish on the growth of Gracilaria sp. and (2) the nutrient content of the water. This study used an experimental method with two treatments and three replications using six (6) earth ponds: Gracilaria sp. monoculture and polyculture with milkfish. Growth and nutrient content (nitrate and phosphate) data were analyzed using a t-test. The results of the study showed that the growth of Gracilaria sp. polyculture with milkfish provided absolute growth of 3615.89 ± 330.84 g; Relative Growth Rate (RGR) 180.79 ± 16.54 %; and Specific Growth Rate (SGR) 2.29 ± 0.13 %/day were significantly higher, compared with the growth of Gracilaria sp. monoculture. Monoculture Gracilaria sp showed absolute growth of 1912.71 ± 386.94 g; RGR 95.64 ± 19.35 %; and SGR 1.48 ± 0.22 %.day-1. Nutrient content in polyculture pond waters revealed nitrate 1.08 ± 0.09 mg.L-1 and phosphate 0.18 ± 0.04 mg.L-1 were significantly higher compared to nutrient content in monoculture pond waters which were nitrate 0.71 ± 0.22 mg.L-1 and 0.07 ± 0.04 mg.L-1. Polyculture Gracilaria and seaweed provide higher production due to the enrichment from nutrient content.

Economic evaluation of Arapaima gigas production in earth ponds: Case study of a small fish farm at San Martin-Peru

Arapaima gigas is an Amazon specie with great commercial demand and high production performance, but its feasibility is variable. The current study aimed to perform an economic evaluation of the production of A. gigas on earth ponds in San Martin, Peru. Three hundred young fish (19 ± 0.01 cm and 68.32 ± 0.01 g) were stocked (ten fish·m–2 in three ponds of 1000 m2 for 390 days). Growth performance was evaluated based on the survival, feed conversion rate (FCR), final mean weight, final average length, weight gain, specific growth rate, and productivity. Investment, costs, indicators, and feasibility were determined. Arapaima gigas has a profitable production performance in earth ponds in Peru, fish presented a weight gain (kg) of 11 kg in 390 days and productivity of 3,265.70 kg·ha–1. Food and fingerlings were the most important items in the production cost. This production is a low-investment and high-profitable business, with an incremental investment of US$3,287.15, profit of US$ 3,977.38·cycle–1, Net Present Value of US$ 22,149.37, and an Intern Rate Return of 32.28%. This could be a way of increasing the income of small producers through the diversification of their production.

Production techniques and environmental management in frog farming in Dong Thap province

This study surveyed 40 farmers on their production techniques and environmental management in frog farming in Cao Lanh and Thap Muoi district, Dong Thap province, and the results showed that frogs were cultured in tarpaulin tanks (82.5%), hapas in an earth pond (35%), and earth ponds (2.5%). Most of the farmers (82.5%) had a combination of breeding frogs and production frogs to be self-sufficient froglet. Stocking density is high with 146.3±92.3 individuals/m2 for production frogs and 51.6±35.7 individuals/m2 for breeding frogs. The culture duration of froglet was an average of 33.6±8.8 days/crop (9.3±2.4 crops/year) and production frogs was 64.7±9.7 days/crop (5.1±0.6 crops/year). Frogs were raised with a commercial pellet feed of many brands. The diseases occurred in tadpole and froglet phase (2-30 days), including septicemia diseases (57.5%), red thighs (45%), white bodies (35%), blindness (35%), digestive disorders (20%), and flatulence (15%). The disease causes were mainly due to poor water quality, weather change, feed, and high stocking density. However, few farmers had treated farming system (37.5%), supplemental water (27.5%), and monitored and controlled water quality. There were 60% of households to discharge wastewater directly into rivers and canals. Some farmers have used nutrient for wastewater from frogs with combining frog with fish (27.5%), or discharging wastewater into ditches gardens, fields (7.5%), settling pond (5%), which have helped to increase income and protect the environment. Therefore, farmers need to be supported with knowledge of farming technique and environmental management from the local government.

Investments in small retention as a factor influencing land-use changes. A case study of Poland

Building permit decisions are one of the most important elements of the investment process in Poland. It should be noted that water reservoirs influence the diversification of landscapes by increasing their attractiveness in both urban and rural areas. The article aimed to verify the relationship between the changes in land-use development and investments related to small retention. Another goal was classifying objects for which building permits have been obtained and registered. Changes in land-use development associated with the introduction of ponds, which blend in with the landscape, are desirable from the perspective of retaining water resources in urban and rural ecosystems. The research methodology was based on spatial data and included statistical analyses in three regions: Mazowieckie, Lodzkie and Swietokrzyskie. Studies carried out in these regions showed a spatial correlation associated with investments in small retention. The research used methods of the global I Moran statistic and local Moran statistics. The data used in the study came from the Register of Applications, Decisions and Notifications, made available by the Main Office of Construction Site. The research indicates clusters of investments in small retention in analysed regions. The majority of investors are residents who invest in earth ponds. The study shows that investment in small retention is connected with ecosystem services.

A Smallholders’ Mariculture Device for Rearing Seafood: Environmentally Friendly and Providing Improved Quality

The aquaculture industry in Taiwan grosses more than USD 1.1 billion annually; however, it also generates considerable waste discharge (causing eutrophication in estuarine and coastal waters) and heavy groundwater withdrawals (causing land subsidence in coastal areas). Many aquaculture facilities using earth ponds are affected by benthic algae, resulting in an earthy odor, and fixed-cage farms are difficult to relocate during cold weather events. In this study, we tested small-scale (~15 ton) mobile cage tanks for the nearshore rearing of white shrimp and grouper in the Yung-An district of Kaohsiung, Taiwan. At the conclusion of the mariculture experiment, the content of free amino acids in shrimp and groupers reared in our mobile tanks surpassed that in animals reared locally in traditional earthy ponds. In a blind taste test involving 42 volunteers, groupers reared in mobile cage tanks were deemed more palatable than those raised in ponds. Our results demonstrate that small-scale mobile cage tanks are a feasible approach to the sustainable rearing of high-quality shrimp or fish. Note that wastewater from the mobile tanks is easily diluted by seawater, thereby reducing the likelihood of eutrophication in coastal regions. The proposed system could also be used for recreational fishing activities to increase income for smallholders of fishermen and/or aquaculture farmers.

Biorefining and biotechnology prospects of low-cost fish feed on Red tilapia production with different feeding regime

This study aimed to decrease the production cost and increase tilapia yield with a feeding regime. The trial was divided into four treatments with three replications followed by the feeding frequency in monosex male Nile tilapia with an average initial weight of 17.86–18.40 g/fish cultured on a net cage in the earth pond for three months. Fish fed with 18% protein supplemented with 5% protein concentrate. The first month was used by 32% protein feed twice per day (T1, control), fish were fed twice per day every other week (T2), fish were fed twice per day per week (T3), and fish were fed twice per day per week (T3). Fish fed twice per day per week are now fed twice per day (T4). These results revealed that T2 fish had the highest growth performance and most protein consumption (P < 0.05). Moreover, fish in T2 has the lowest production cost and highest benefit-cost ratio (B/C ratio) (P < 0.05).The highest percentage of edible meat in T2 was P < 0.05, but the proximate analysis was close for all three groups (P > 0.05). As a result, it is necessary to ensure that Nile Tilapia culture has sufficient protein and nutrition control. This will allow for both high growth and low cost.Fish health and quality were positively affected by a perfect protein-containing feeding regimen. In fish fed with 18% protein and 5% protein concentrate for the first month, followed by 32% protein feed twice every other day and then with 18% protein.

Elemental Fingerprinting of Wild and Farmed Fish Muscle to Authenticate and Validate Production Method.

In the context of expanding fish production and complex distribution chains, traceability, provenance and food safety tools are becoming increasingly important. Here, we compare the elemental fingerprints of gilthead seabream (Sparus aurata) muscle from wild and different aquaculture productions (semi-intensive earth ponds and intensive sea cages from two locations) to confirm their origin and evaluate the concentrations of elements with regulatory thresholds (Cu, Hg, Pb and Zn). Using a chemometric approach based on multi-elemental signatures, the sample origin was determined with an overall accuracy of 90%. Furthermore, in a model built to replicate a real-case scenario where it would be necessary to trace the production method of S. aurata without reliable information about its harvesting location, 27 of the 30 samples were correctly allocated to their original production method (sea-cage aquaculture), despite being from another location. The concentrations of the regulated elements ranged as follows: Cu (0.140–1.139 mg/Kg), Hg (0–0.506 mg/Kg), Pb (0–2.703 mg/Kg) and Zn (6.502–18.807 mg/Kg), with only Pb presenting concentrations consistently above the recommended limit for human consumption. The present findings contribute to establishing elemental fingerprinting as a reliable tool to trace fish production methods and underpin seafood authentication.

New indices for the diagnosis of fish welfare and their application to the grass carp ( Ctenopharyngodon idella ) reared in earthen ponds

Animal welfare is an issue of increasing importance in global aquaculture. However, the incorporation of this issue into routine aquaculture operations is a challenge. A possible path to change this is the recognition, identification and addressing of the ecological, physiological, nutritional, behavioural and psychological needs of fish. The present study uses grass carp (Ctenopharyngodon idella), the world's most widely produced fish species, and earth ponds, the world's most widely used production system in fish farming worldwide, as the basis for developing indices to assess and monitor the welfare of farmed fish. The proposed indices were structured based on specific welfare indicators, reference values, individual weights and associated scores to address the Animal Welfare Five Freedoms and a Life Worth living concepts. Two types of indices were proposed: (1) partial welfare indices (PWIx), specific to each of the four observed/analysed freedoms; (2) general welfare index (GWI), which simultaneously summarizes the addressees' freedoms in a single variable. Both indices range from 0 (critical welfare impairment) to 1.0 (minimal risk of welfare impairment). The study was based on a comprehensive systematic review of the literature using the PRISMA method. The proposed indices were based on 10 environmental indicators, nine indicators for health, five for nutrition and four for behaviour. The PWIx can be used to determine how each category of indicators contributes to the GWI, which defines the level of fish farm welfare at a given point in time and which needs are affected or met during a production cycle.

Possible Acquisition of ESBL-mediated Antimicrobial Resistance by Farmers from Aquatic Reservoir used for Bathing and Cleaning of Water Buffalos (Bubalus bubalis) with Intestinal Carriage of ESBL Producing Escherichia coli

Little information is available on the risk of human subjects for acquisition of antimicrobial resistance (AMR) from aquatic environment other than those treated with antimicrobials for aquaculture. Carriage of extended-spectrum beta-lactamase (ESBL) and carbapenemase categories of AMR by enteric bacteria in livestock have been frequently reported. Dissemination of these categories of AMR to the environment thus poses a threat for their transmission to farmers engaged in livestock care posing a severe public health hazard. A study on the prevalence of ESBL- and carbapenemase-mediated AMR among Escherichia coli isolated from earth pond environment used for bathing and cleaning of buffalos (Bubalus bubalis) and from human subjects engaged in such activity revealed isolation rate of ESBL positivity to be higher in human subjects engaged in washing and bathing of buffalos (37.5%) compared to those without engagement in such activities (20.7%) with CTX-M type ESBL, a group of class A ESBL, as the predominant molecular type (97.4%). While no carbapenemase positivity could be detected among E. coli isolated from pond environment or buffalos, small percentage of carbapenemase could be detected among the E. coli isolated from human subjects although the risk was not higher than those not associated with bathing and cleaning of buffalos. Bathing and cleaning of buffalos could potentially facilitate transmission of ESBL resistance from livestock to human subjects in pond environment.

Site specific bacterial load, enterobacterial occurrence and antibiotic susceptibility patterns in Nile Tilapia (Oreochromis niloticus), water and sediment from earthen aquaculture ponds

INTRODUCTION: Aquaculture is the most rapidly growing food production sector. The intensive nature of freshwater fish aquaculture is associated with practices which predispose aquaculture produce to bacterial contamination. To prevent losses due to bacterial diseases, there has been an increase in the uncontrolled use of antibiotics, a good prerequisite to the development of antibiotic resistance - a major global problem. Hence, we aimed at investigating the enterobacterial diversity and occurrence of antimicrobial resistance in Nile Tilapia and its earth pond environment. METHODS: fish samples, pond water and sediments were collected from four earthen ponds located at Yato, Cameroon, in three different collection trips for Total Heterotrophic Aerobic Plate Count (THAPC) and enterobacterial isolation. Bacterial characterisation and antibiotic sensitivity were assessed using the API 20E kit and the standard Kirby-Bauer disc diffusion method, respectively. RESULTS: THAPC ranged from 1.0 x 102 to 3.0x109 CFU/mL across samples and within each pond, bacterial loads were highest in fish parts and lowest in the surrounding environment. Sixteen enteric genera and 24 species were identified, with Escherichia coli (16.49 %) and Enterobacter cloacae (12.37 %) being most abundant, especially in the gills and intestines. The highest degree of resistance was observed with Amoxicillin and Ampicillin, with more than 60 % of tested isolates being resistant. Multiple drug resistance was also observed in all sample types, ponds and collection time points, and highest in fish parts. CONCLUSION: this study re-emphasizes the potential of freshwater fish aquaculture as carrier of human pathogenic and multiple drug resistant bacteria.

Growth, Fry Production, and Cost Analysis for Sabaki Tilapia Production Systems

AbstractThis study estimated the survival, growth, fry production, and farming cost of Sabaki Tilapia Oreochromis spilurus broodstock in the following five outdoor production system types: arena, raceway, cage, concrete pond, and earth pond. The experiments were performed in triplicates in the year 2020 with 300 broodstocks per production system. Survival rate, growth performance, fry production, and farming cost were monitored. Our results indicated that Sabaki Tilapia fry production was feasible under high‐salinity conditions in the production system types studied; however, the systems showed significant differences in terms of survival rate, growth performance, and fry production. The arena and raceway systems were the most effective and exhibited the highest fry production.

Invasive Amirthalingamia macracantha (Cestoda: Cyclophyllidea) larvae infecting tilapia hybrids in Israel: a potential threat for aquaculture.

Larvae (metacestodes) of gryporhynchid tapeworms (Cestoda: Cyclophyllidea) are reported for the first time from the liver of tilapia hybrids (Oreochromis aureus × O. niloticus) reared in earth ponds in northeastern Israel (along the Jordan River). This is the first record of Amirthalingamia macracantha (Joyeux & Baer, 1935), a parasite of cormorants (Phalacrocoracidae), outside Africa and outside the tropics. Larvae found in the liver of tilapias (Cichlidae) were identified to species level because they possessed 20 massive rostellar hooks of 3 types, with the 4 largest hooks measuring almost 500 µm. Molecular data confirmed species identification. The possible route of introduction to Israel of this African parasite, which is large (length up to 2 cm) and potentially pathogenic for cultivated tilapias, is discussed.

SCALE EFFICIENCY IN TAMBAQUI FARMING IN EARTH PONDS IN THE METROPOLITAN REGION OF MANAUS-AM

This study aimed at determining the scale efficiency in tambaqui production in earth ponds developed in the metropolitan region of Manaus-AM. Eleven fish farms were analyzed and were sampled data regarding its characteristics, productive data, disbursement, infrastructure value and production value, in order to calculate the total operational cost and profitability indexes. For the DEA was applied the VRS/BCC (variable return of scale) model with input orientation, using three inputs (total operational cost, feed value, and depreciation, all in BRL($) per kilo) and one output (annual gross revenue, in BRL($) per hectare). The study showed that 80% of the fish farms (DMUs) had increasing returns to scale, with scale efficiencies lower than 65%, affected by lack of profitability, low technical and marketing knowledge, high production cost (R$ 4.50 kg-1 ) and low average sales price (lower than R$ 5.00 kg-1).

Simultaneous detection of scale drop disease virus and Flavobacterium columnare from diseased freshwater-reared barramundi Lates calcarifer.

Freshwater farming of barramundi Lates calcarifer in Thailand is a growing sector in aquaculture, but mortalities due to infectious diseases are still a major threat to this industry. In 2018, an episode of severe mortality in juvenile barramundi was noted in a freshwater earth pond site. Fish presented with severe gill necrosis, as well as severe cutaneous hemorrhages, scale loss, and discoloration at the base of dorsal fin (saddleback lesions). Histopathology revealed extensive necrosis of skeletal muscle and gill filaments, as well as basophilic inclusion bodies and megalocytosis in muscle, gill, liver, and kidney. Scale drop disease virus (SDDV) infection was subsequently confirmed by virus-specific semi-nested PCR. Further, DNA sequences of the viral major capsid protein (MCP) and ATPase genes had a respective homology of 99.85 and 99.92% with sequences of SDDV infecting barramundi in saltwater culture. Gill necrosis and saddleback lesions are not typical lesions associated with scale drop syndrome. Their presence was explained by Flavobacterium columnare isolation from the gill, followed by positive F. columnare-specific PCR. To our knowledge, this is the first report of SDDV-associated mortality in freshwater-farmed barramundi. Furthermore, this mortality presented as a concurrent infection with SDDV and F. columnare, with typical lesions of both infections.

Gill amoebae from freshwater rainbow trout (Oncorhynchus mykiss): In vitro evaluation of antiparasitic compounds against Vannella sp.

Gill parasitic infections challenge farming of rainbow trout (Oncorhynchus mykiss, Walbaum) in freshwater facilities. Apart from flagellates (Ichthyobodo, (Pinto) and ciliates (Ichthyophthirius (Fouquet), Ambiphrya (Raabe), Apiosoma (Blanchard), Trichodinella (Sramek-Husek) and Trichodina (Ehrenberg)), we have shown that amoebae are prevalent in Danish trout farms. Gills were isolated from farmed rainbow trout in six fish farms (conventional and organic earth pond and recirculated systems) and placed on non-nutrient agar (NNA) moistened with modified Neff's amoeba saline (AS) (15°C). Gill amoebae from all examined fish colonized the agar and were identified based on morphological criteria showing species within the genera Trinema (Dujardin) (family Trinematidae), Vannella (Bovee) (family Vannellidae). In addition, hartmannellid amoebae were recorded. We established a monoculture of Vannella sp., confirmed the genus identity by PCR and sequencing and performed an in vitro determination of antiparasitic effects (dose-response studies) of various compounds including sodium chloride (NaCl), hydrogen peroxide, peracetic acid, formalin, aqueous garlic and oregano extracts and a Pseudomonas H6 surfactant. All amoebae were killed in concentrations of 16.90mg/ml (garlic), 17.90mg/ml (oregano), NaCl (7.5mg/ml), hydrogen peroxide (100µg/ml), peracetic acid (0.03µg/ml), formaldehyde (25µg/ml) and the Pseudomonas H6 surfactant (250µg/ml).

Growth performance, gonad development and nutritional composition of Chinese mitten crab Eriocheir sinensis selected for growth and different maturity time

Chinese mitten crab is an important aquaculture species in China, and a selective breeding program has been operated for the economic traits of growth and different maturity time of this species since 2010. This study aimed to evaluate the growth performance and nutritional composition of two selected lines, i.e. early-maturing strain (EM) and late-maturing strain (LM), and control population without genetic selection cultured in identical outdoor earth ponds. The results showed that: (1) The average body weight of two selective lines were always higher than the control during the pond culture of adult E. sinensis. Among two selected lines, the average body weight of the EM was relatively higher than that of LM between May and July, but EM had the relatively lower body weight than the LM during the late culture stage between Sept and Nov. (2) Compared to control population, despite of males or females, pubertal molting of EM and LM were occurred earlier and later respectively than that of control both, and there were around 15–20 days earlier for the pubertal molting of EM. For gonadosomatic index (GSI), EM had the higher GSI than LM and control populations between Oct.10 and Nov. 20 for males as well as between Sep.20 and Oct. 30 for females during the gonadal development cycle; (3) For the edible indices of adult crabs at the harvest, EM had the significantly higher of GSI and total edible yield (TEY) than LM, and control had the lowest hepatosomatic index among three populations (P < .05); (4) For proximate composition, three populations generally had the close proximate contents in three edible tissues (muscle, gonads and hepatopancreas), but female LM had the significantly lower lipid contents in both hepatopancreas and gonad than EM and control (P < .05); (5) As for fatty acid, female LM had the higher percentages of C18:3n3, total polyunsaturated fatty acids (ΣPUFA) and total long chain polyunsaturated fatty acids (Σ LC-PUFA) in muscle and hepatopancreas than the other populations, while EM had the significantly higher percentages of C22:6n3 in gonads than LM for both males and females; (6) Selective breeding had no apparent effects on amino acid compositions and contents of muscle and all essential amino acid scores were above 100. In summary, selective breeding provides a useful means for the improvement of growth and time of pubertal molting for E. sinensis, but has limited effects on their nutritional composition.

Parasites in fishery products - Laboratorial and educational strategies to control

The presence of parasites, including Anisakis spp. larvae in fishery products jeopardizes their safety and quality, which is a matter of concern and a challenge for official authorities, as well as for food businesses operators, and a potential health risk to consumers. In the last ten years, a total of 2367 samples from different seafood products, whole fish (n = 1022), fish steaks (n = 417), fish fillets (n = 46), roes (n = 11), canned fish fillets (n = 35), fish fingers (n = 705), and ready-made Japanese dishes (n = 131) were analysed at the Laboratory of Pathology of Aquatic Animals of IPMA. From the fishery products analysed, a total of 4643 visible Anisakis larvae were collected from eighteen different species of whole fish and there were two cases with numerous larvae, although live larvae were only found in fresh fish. Anisakis larvae (n = 523) were collected from fish steaks, (n = 20) from frozen fish fillets, (n = 7) from five different brands of fish fingers, (n = 2) from two cans of cod fillets and numerous viable larvae were collected from the serosae of European hake fresh roes. The great majority of the larvae were identified as L3 Anisakis larvae. Plerocercoids of Gymnorhynchus gigas and Hepatoxylon trichiuri and the myxosporean, Kudoa spp. were also observed and led to the withdrawal of the fish from the supply chain. No Anisakis larvae were collected from the farmed fish as well as from the ready-made Japanese dishes. However, Kudoa hexapunctata spores were observed in the tuna muscle from one sashimi meal. The data shows that fresh fish with live Anisakis larvae have been marketed. Educational strategies concerning parasites were implemented with workshops, “Parasites in Fishery Products” to train and instruct professionals within the fish supply chain and training activities for target groups, including children, were promoted, to raise awareness of this issue. One of the most relevant outcomes obtained was the improvement of implementation of parasite identification techniques in the industry, including private labs, and consequently: (1) decrease in the parasitological analysis number of common parasites and (2) reduction in the analysis of anatomical structures confused with parasites requested to IPMA's Laboratory of Pathology. In addition, the practice of freezing trash fish used to feed farmed fish, and the placement of nets at the water entry points of the earth ponds, in order to avoid wild fish introduction, were simple and important measures that were implemented to prevent Anisakis and other parasites entering fish farms. The two complementary parts of this work, laboratorial activities used to support the fish supply chain, and the educational strategies concerning parasites, have made an essential contribution towards the availability of data for assessing and minimizing the level of risk associated with fish consumption, and have particularly increased and improved awareness, regarding foodborne parasites.

Vitiligo Treated by Soaking in an Earth Pond Containing KELEA Activated Water

Vitiligo completely regressed in two patients who spent several hours for each of three consecutive days in a pond that contains water with a heightened quantity of a non-thermal activity attributed to a natural force called KELEA (Kinetic Energy Limiting Electrostatic Attraction). KELEA is further defined as the source of cellular energy, which comprises the alternative cellular energy (ACE) pathway. The ACE pathway differs from the cellular energy obtained from food metabolism. It is expressed as an added dynamic (kinetic) quality of the body’s fluids. The body utilizes the ACE pathway in both chemical and electrical cellular reactions, including the recovery and regenerative processes following cellular damage. The ACE pathway supports the higher levels of brain functioning including the likelihood of improving upon the presumed KELEA receiving capacity of the brain. The ACE pathway can also limit the triggering and maintenance of the inflammatory response. Each of these activities is presumably involved in the clinical regression of the vitiligo in the two patients. Enhancing the ACE pathway may provide an effective therapeutic approach for many patients with vitiligo.