Early Stages Of Selection Research Articles

The Reality of Innovation Management in Higher Education Institutions from Innovative Students’ Point of View

Innovation management plays a crucial role in harnessing innovations for economic development. Higher education institutions are considered one of the fundamental stages for embracing and supporting student innovations, especially in the early stages of ideation, selection and experimentation. Within this context, the current study aimed to understand the reality of innovation management in Omani higher education institutions at the pre-innovation stage. The study employed a descriptive methodology using a qualitative phenomenological research design. Data was collected through semi-structured interviews with a sample of 30 participants from two government universities and three private universities. The study utilised thematic analysis for data interpretation. In its findings, the study discusses the current reality of the management of the pre-innovation stage in Omani higher education institutions through five themes: announcing competitions, team formation, supervisory role, exploration of innovative ideas, and policies and regulations. The findings revealed that, despite efforts made by universities, there is a lack of planning or a defined roadmap for the innovation process. The study recommends implementing supportive policies and regulations for innovation, placing more emphasis on planning in the pre-innovation stage and adjusting supervision for students according to their innovative areas. KEYWORDS Innovation management, higher education, pre-innovation stage, innovation stage, innovation policies, Sultanate of Oman.

Toward a more pre-emptive approach to managing work emotions and emotional labor

PurposeTo propound a broadened perspective on emotional labor management by exploring mitigatory approaches that could be pre-emptively deployed prior to actual episodic experiences of emotional dissonance and their associated negative consequences (e.g. burnout). At present, the management of emotional labor appears to skew toward reactive measures, such as deploying employee assistance programs (EAPs) to assist overwhelmed employees in coping better with their emotional demands, reducing job-related emotional demands or a combination of both.Design/methodology/approachIntricate processes of emotion emergence and established literature on emotion regulation are considered. By conceptualizing emotion emergence as a process entailing situation, attention, appraisal and response, current efforts can be seen as primarily acting upon the late stages of this process. General emotion regulation strategies that act upon more upstream processes are then considered and applied to the specific context of emotional labor.FindingsPre-emptive steps could be taken from the early stages of job selection as well as personnel selection and assessment through systematic and concerted efforts in identifying job-related emotional demands (e.g. specific display rules, frequency and intensity). Formal job descriptions could then reflect these demands to better facilitate self-selection processes. Additionally, considering these identified parameters as personnel selection and assessment criteria could further enhance person-job fit in terms of emotional congruency. For current hires, pre-emptive steps could also be taken to subliminally modulate their emotional emergence trajectory toward more job-congruent emotions. Collectively, these steps may facilitate the pre-emptive reduction of emotionally dissonant work episodes and bear substantive potential to be deployed synergistically with current, more reactive measures.Originality/valueThis paper offers a broadened perspective on emotional labor management. Through considering intricate processes of emotion emergence and established literature on emotion regulation, a pre-emptive perspective toward managing work emotions and emotional labor is propounded. It is believed that the synergistic incorporation of these pre-emptive management approaches with current strategies (e.g. reducing emotional demands, EAPs, etc.) would holistically allow for greater amelioration of this debilitating issue. Finally, it is hoped that this paper could serve as a primer for future research and discourses to be conducted, such that our arsenal available for combating emotional labor could be substantively expanded to holistically target all stages of the emotion emergence process.

Polymorphism of growth hormone (GH/HaeIII) gene and its association with the performance of merino cross rams

Merino cross sheep (75% Merino × 25% Garut) are introduced by the government of Indonesia for meat production purposes. The present study aimed to determine the polymorphism in the exon 2 region of the growth hormone (GH) gene (422 bp) in Merino cross rams using the PCR-RFLP technique and to analyze its relationship with body weight and body measurements of the rams. A total of 145 rams aged one-year-old with an average body weight of 29.08 ± 7.96 kg from the breeding station in West Java, Indonesia were considered as the experimental animals. It was indicated that a missense mutation of c.55G > A (p.G19S) was detected in the target sequence of the GH gene in Merino cross rams. The PCR-RFLP analysis in the GH gene of Merino cross with HaeIII restriction enzyme (GH/HaeIII) was observed in a moderate category with a polymorphic informative content (PIC) value of 0.22. Therefore, the G allele was more frequent than the A allele (0.85 versus 0.15). Furthermore, the genotype AA was not present among the sheep that were part of the study. However, the polymorphism of p.G19S was found to have no significant association with birth weight and body measurements in one-year-old Merino cross sheep. However, the GH/HaeIII gene in Merino cross rams exhibited polymorphism, primarily with two genotypes: GG (wildtype) and GA (carrier). The G allele was identified as the dominant allele in the ovine GH gene, occurring with a frequency of 0.85. Importantly, the polymorphism of the GH/HaeIII gene was significantly linked to birth weight and chest depth in one-year-old Merino cross rams. These findings provide preliminary insights that could potentially aid in the early stages of molecular selection for Indonesian Merino cross sheep.

DEVELOPING MULTILINE COWPEA VARIETIES ADAPTED TO LOCAL CROPPING SYSTEMS AND SPECIFIC FARMERS’ PRODUCTION GOALS THROUGH PARTICIPATORY BREEDING

<p><strong>Background</strong><strong>.</strong> Decentralized participatory breeding approach facilitate the development of varieties better suited for a diversity of farmers' contexts. To enhance breeding efficiency, formal methodological settings are needed to include, at early stage of selection, particular local practices and farmers’ preferences. <strong>Objective</strong><strong>.</strong> Evaluate how location, farmers’ profile and local cropping system could be taken into account to optimize selection of cowpea varieties in a decentralized context. <strong>Methodology:</strong> Twenty-two candidate multiline varieties of cowpea (<em>Vigna unguiculata L. </em>Walp) were developed from a biparental family of recombinant inbred lines. These varieties were subjected to selection under edaphic and climatic conditions of three locations and two cropping systems (intercropping <em>versus</em> sole cropping). Trials were carried out in collaboration with farmers' federations. Participatory evaluations were conducted over two years by the three groups of farmers defined by the following production goals: grains production as priority (“Grain priority profile”), fodder as priority (“Fodder priority profile”) and grain quality as priority (“Food processing priority profile”). <strong>Results</strong><strong>.</strong> The statistical analysis supported significant effects of location and cropping system on the agronomic traits, with interactions effects involving the variety. A strong correlation was observed between the varietal choices of the two farmers’ profiles which prioritized respectively grain and food processing. These farmers preferred varieties with higher grain yield and best grain quality. Farmers who prioritized fodder preferred more specific varieties, characterized by highest haulm yield. <strong>Implications.</strong> The clustering of farmers into specific profiles is an efficient method which allow expressing their diversified production goals through participatory evaluation. This led to more specific varietal choices for each of the profiles. <strong>Conclusion</strong><strong>.</strong> This study set cropping systems and farmer profiles as formal design factors at early breeding stage. These factors acted efficiently to support the process of varietal choice. The participatory approach highlighted the congruence and complementary between farmers’ and researchers’ knowledges. It helped identifying, for intercropping and sole cropping systems respectively, the most suitable varieties preferred by each of three respective farmers’ group.</p>

Bond strength and marginal adaptation of resin composites and correlations with clinical results

ObjectivesDue to innumerable confounding factors and a high number of types and brands of dental restorative materials, the clinical performance of restorative materials are sought predicted by various in vitro tests. However, only few such tests have been found to correlate well with clinical findings. Thus, the present study determined the in vitro dentin bond strength and marginal adaptation of Class II restorations and correlated the results to their clinical outcomes. MethodsDentin bond strength (µTBS and µSBS) and marginal gap formation of Class II restorations (replica technique and SEM) were measured after 24 h and 6 m water storage using eight combinations of adhesive and resin composite. Clinical outcomes (mean survival time, Hazard Ratio, annual failure rate; n = 10.695) were gained from a data set of a retrospective multicenter study of direct restorations. ResultsSignificant differences were found for dentin bond strength and marginal gap formation between the restorative material groups, and negative effects of long-term storage were observed. µTBS correlated significantly with certain clinical outcomes of Class I restorations, while µSBS correlated with certain clinical outcomes of Class II, III, IV and V restorations. Marginal gap formation in enamel and number of paramarginal fractures correlated with certain clinical outcomes of Class II restorations. SignificanceUsing the same restorative materials in vitro as in vivo, gave significant, but weak correlations between in vitro bond strength or marginal adaptation and clinical outcomes, lending support to the use of in vitro tests in early stages of material selection.

Anthropometric Profile of Elite Male and Female Cadet Taekwondo Competitors

Introduction: Taekwondo has developed into an Olympic sport, which means that a thorough understanding of the main Taekwondo athletes and competitors' characteristics is necessary. This study investigated the fundamental anthropometric measurements of high-level Taekwondo athletes, with a particular focus on disparities between genders. The objective of this study was to assess and contrast the physical attributes of elite Taekwondo athletes, specifically examining the differences between males and females. Methods: A group of 28 highly skilled Taekwondo athletes, consisting of 14 female and 14 male cadets participated in this study. Results: The results showed a significant difference between male and female competitors' anthropometric variables, most notably in skinfold thickness and fat tissue proportion (p≤ 0.01). Conclusion: Accordingly, these data can help taekwondo specialists in the early stages of selection, especially when advanced examinations are impossible. Further studies profiling this group of responders should include more characteristics to help practitioners recognize talents and create training program goals.

Bacterial Leaf Spot Susceptibility Screening of Chili Pepper Cultivars Using qPCR Determination of Xanthomonas euvesicatoria pv. euvesicatoria Titers.

Bacterial leaf spot is a serious disease of chili pepper (Capsicum spp.) caused by Xanthomonas euvesicatoria pv. euvesicatoria. Conventional resistance screening is time and resource intensive. It was considered that a quick and simple determination of cultivar susceptibility could be achieved through estimating bacterial titers of inoculated plants. A SYBR quantitative polymerase chain reaction (qPCR)-based assay was compared with conventional PCR, then used to detect and enumerate pathogen titers in serial dilutions and DNA extracted from infected plant leaves. The qPCR detection limit was approximately 1 CFU µl-1, 10 times more sensitive than conventional PCR. A linear correlation (R2 = 0.994) was obtained from the standard curve comparing plate-truthed serial dilutions of the pathogen with the qPCR cycle threshold. Six strains were used to inoculate cultivars Hugo and Warlock. One strain, X. euvesicatoria pv. euvesicatoria BRIP62403, was consistently the most virulent based on visual symptoms and pathogen titers in planta inferred by qPCR performed on DNA extracted from infected leaves 2 and 6 weeks postinoculation. Visual observations 6 weeks after inoculation were highly correlated (R2= 0.8254) to pathogen titers. The qPCR method was used to categorize 20 chili pepper cultivars 2 weeks after inoculation. A high positive correlation (R2 = 0.6826) was observed between visual scoring and pathogen titers from 20 chili pepper cultivars, facilitating categorization of susceptible, intermediate, and resistant cultivars. The qPCR approach developed here facilitates susceptibility screening of chili pepper cultivars at an early stage of selection and could be readily adapted to a range of other pathosystems.

Evaluation of Sugarcane Families and Identification of Promising Families for Cane Yield and Juice Quality Traits in Early Selection Stages

The utilization of superior parental lines and identification of promising families enhance the genetic gain in sugarcane. To identify the promising families, 1620 progenies derived from twenty two crosses involving genetically diverse historical parents were evaluated for cane yield and juice quality attributing traits. The mean number of millable canes per clump (9.00), cane girth (2.32 cm), millable cane height (217.00 cm) and Brix% in juice (20.79%) were recorded. The significant variance, high broad-sense heritability and genetic advance over mean suggested that number of millable canes per clump, single cane weight, millable cane height, Pol% in juice and Brix% in juice are suitable for family evaluation. Based on progeny performance, families of Co 86032 × CoVC 14061 and Co 0312 GC, were found significantly superior compared to most popular clonal check, Co 86032 for number of millable canes per clump, similarly, CoVC 14062 × Co 89003 and Co 86002 × ISH 69 for cane girth, and CoVC 14062 × Co 89003, Co 11015 and Co 87015 for CCS% in juice content at early selection stages. Among 22 families studied, the four families viz., Co 86032 × CoVC 14061, CoVC 14062 × Co 89003, CoVC 14062 GC, Co 87015 GC and Co 11015 GC, produced higher percentage of population selection with acceptable range for commercially important traits (Brix% in juice, cane yield components). These identified promising families can be further exploited in sugarcane varietal improvement.

The Application of Peptide Nucleic Acids (PNA) in the Inhibition of Proprotein Convertase Subtilisin/Kexin 9 (PCSK9) Gene Expression in a Cell-Free Transcription/Translation System.

Proprotein convertase subtilisin/kexin 9 (PCSK9) is a protein that plays a key role in the metabolism of low-density lipoprotein (LDL) cholesterol. The gain-of-function mutations of the PCSK9 gene lead to a reduced number of surface LDL receptors by binding to them, eventually leading to endosomal degradation. This, in turn, is the culprit of hypercholesterolemia, resulting in accelerated atherogenesis. The modern treatment for hypercholesterolemia encompasses the use of biological drugs against PCSK9, like monoclonal antibodies and gene expression modulators such as inclisiran-a short, interfering RNA (siRNA). Peptide nucleic acid (PNA) is a synthetic analog of nucleic acid that possesses a synthetic peptide skeleton instead of a phosphate-sugar one. This different structure determines the unique properties of PNA (e.g., neutral charge, enzymatic resistance, and an enormously high affinity with complementary DNA and RNA). Therefore, it might be possible to use PNA against PCSK9 in the treatment of hypercholesterolemia. We sought to explore the impact of three selected PNA oligomers on PCSK9 gene expression. Using a cell-free transcription/translation system, we showed that one of the tested PNA strands was able to reduce the PCSK9 gene expression down to 74%, 64%, and 68%, as measured by RT-real-time PCR, Western blot, and HPLC, respectively. This preliminary study shows the high applicability of a cell-free enzymatic environment as an efficient tool in the initial evaluation of biologically active PNA molecules in the field of hypercholesterolemia research. This cell-free approach allows for the omission of the hurdles associated with transmembrane PNA transportation at the early stage of PNA selection.

The dynamics of statistical learning in visual search and its interaction with salience processing: An EEG study

Visual attention can be guided by statistical regularities in the environment, that people implicitly learn from past experiences (statistical learning, SL). Moreover, a perceptually salient element can automatically capture attention, gaining processing priority through a bottom-up attentional control mechanism. The aim of our study was to investigate the dynamics of SL and if it shapes attentional target selection additively with salience processing, or whether these mechanisms interact, e.g. one gates the other. In a visual search task, we therefore manipulated target frequency (high vs. low) across locations while, in some trials, the target was salient in terms of colour. Additionally, halfway through the experiment, the high-frequency location changed to the opposite hemifield. EEG activity was simultaneously recorded, with a specific interest in two markers related to target selection and post-selection processing, respectively: N2pc and SPCN. Our results revealed that both SL and saliency significantly enhanced behavioural performance, but also interacted with each other, with an attenuated saliency effect at the high-frequency target location, and a smaller SL effect for salient targets. Concerning processing dynamics, the benefit of salience processing was more evident during the early stage of target selection and processing, as indexed by a larger N2pc and early-SPCN, whereas SL modulated the underlying neural activity particularly later on, as revealed by larger late-SPCN. Furthermore, we showed that SL was rapidly acquired and adjusted when the spatial imbalance changed. Overall, our findings suggest that SL is flexible to changes and, combined with salience processing, jointly contributes to establishing attentional priority.

Requirements for inventory models of radioactive waste

Abstract. The aim of the final disposal of radioactive waste is to ensure the best possible safety for the long-term protection (i.e. 1 million years) of humans and the environment from ionizing radiation and other harmful effects of this waste. In Germany, the Site Selection Act (StandAG) regulates the procedure for determining the site with the best possible safety for a repository with high-level radioactive waste. One of the mandatory aspects of the StandAG is to perform various preliminary safety assessments in order to assess the expected extent of the safe confinement of the radioactive waste. For this purpose, all information (in particular on the quantity, type, composition and activity) of the waste (i.e. the inventory) is required to conduct the respective safety assessment. On behalf of the Federal Office for the Safety of Nuclear Waste Management (BASE) and within the framework of the research project “Significance of the radioactive waste inventory in the site selection for a repository (InvEnd)”, Brenk Systemplanung GmbH identified the characteristics of the inventory required for the preliminary safety assessments. To determine these inventory characteristics, two main data sources were analysed, namely the generic but host-rock-specific concepts for a German high-level radioactive waste repository and the inventory data and approaches of other countries with technologically developed repository programmes. The identified characteristics can be divided into different groups (e.g. geometric, chemical, radiological and physical) and categories such as primary inventory data that are essential for carrying out the preliminary safety assessments or secondary inventory data which validate the primary inventory data. The evaluation of the impact of the identified characteristics shows that especially data on component-specific chemical composition and component-specific radionuclide inventory have a high impact on the results of safety assessments. These characteristics are, in turn, dependent on other characteristics, such as the irradiation history and the original chemical composition of the fuel in addition to the structural parts of the fuel assemblies. Furthermore, the study assesses uncertainties of the inventory characteristics, such as data uncertainty and conceptual uncertainty. The extent of data uncertainty depends on the inventory characteristic. However, in most cases, only qualitative assessments are possible. Conceptual uncertainties depend on the degree of simplification used to define reference wastes but cannot be quantified yet, as no reference wastes have been defined. The results of this study enable authorities and waste management organizations to identify the key data of high-level radioactive waste inventories that are essential for conducting safety assessments of repositories, even during early stages of site selection and planning.

Development of a Laboratory-Scale Test Methodology for Performance Evaluation of Lubricants for Hot Stamping of an Aluminium Alloy

In hot stamping of aluminium, the need for efficient methods to evaluate, compare, and rank lubricants based on their tribological performance is critical in the early stages of selection. Pilot and simulative testing can be costly, time-consuming, and complex, making it inefficient for initial benchmarking. This work aims to develop a test methodology to assess lubricant performance for hot stamping under key operating conditions without fully simulating the forming process. The proposed method distinguishes the impact of temperature on lubricant degradation, friction, wear response, and cleanability. The tests utilised a conventional hot work tool steel and a 6010S aluminium alloy with two commercially available lubricants: a polymeric lubricant and a lubricant containing graphite. The tribological tests involved a reciprocating, sliding flat-on-flat configuration at two temperatures (100 °C and 300 °C). The methodology showed that the graphite-containing lubricant exhibited over a four times lower friction coefficient than the polymer-based lubricant at 10 wt.% concentration and 300 °C. At 100 °C, both lubricants provide lubrication and can be cleaned, but increasing temperature led to a significant decline of both aspects. The observed temperature range where the lubricants degrade was between 120 °C and 170 °C.

Identifying the Principal Contributing Traits for Commercial Cane Sugar Yield at First Clonal Selection Stage in Sugarcane (Saccharum spp.)

Commercial cane sugar (CCS) yield is the main target product in sugarcane, which depends on the sucrose content and cane yield related traits. Understanding the factors influencing CCS yield would help us to enhance the selection efficiency for sugar yield. A population of 1,080 seedlings derived from nine bi-parental crosses involving Indian Co canes and Co allied hybrids were evaluated in seedling stage. A subset comprised of 270 clones selected from the single stool stage were evaluated in the first clonal trial to identify major component traits of CCS yield in the early stages of selection. Investigations were undertaken in the population to explore the feasibility of employing PCA and regression analyses to provide a basis for selection criteria for identification of sugarcane genotypes that are higher in CCS yield. Comparative performance of families at ground nursery indicated that screening of seedling progenies should be towards the crosses with higher mean value of H.R. brix% and single cane weight to enhance the frequency of superior individuals in subsequent clonal generations. The results of correlation analysis at first clonal stage suggested that yield related traits including tillers at 120 days, single cane weight, number of millable stalks and cane yield are the most important characters that significantly influences the CCS yield. Principal component analysis revealed that four factors could explain approximately 80.40% of the total variation for CCS yield in the population studied. The first factor which accounted for 31.19% of the variation was strongly associated with juice brix%, sucrose%, H.R. brix%, purity% and CCS%, whereas the second component had a positive effect from single cane weight, juice weight and cane diameter explaining 21.74% of total variability. The third factor accounted for 18.97% of variability and had contribution from tillers at 120 days, number of millable stalks and cane yield. Stalk height was independent from other traits which accounted for 8.48% of variation in component 4. Stepwise regression analysis demonstrated that CCS%, cane yield and sucrose % had considerable high effects on commercial cane sugar yield which explained 85.53% (expressed as R2) of variability in clones derived from bi-parental crosses between Indian Co canes.

CCR4 and CCR7 differentially regulate thymocyte localization with distinct outcomes for central tolerance.

Central tolerance ensures autoreactive T cells are eliminated or diverted to the regulatory T cell lineage, thus preventing autoimmunity. To undergo central tolerance, thymocytes must enter the medulla to test their T-cell receptors (TCRs) for autoreactivity against the diverse self-antigens displayed by antigen-presenting cells (APCs). While CCR7 is known to promote thymocyte medullary entry and negative selection, our previous studies implicate CCR4 in these processes, raising the question of whether CCR4 and CCR7 play distinct or redundant roles in central tolerance. Here, synchronized positive selection assays, two-photon time-lapse microscopy, and quantification of TCR-signaled apoptotic thymocytes, demonstrate that CCR4 and CCR7 promote medullary accumulation and central tolerance of distinct post-positive selection thymocyte subsets in mice. CCR4 is upregulated within hours of positive selection signaling and promotes medullary entry and clonal deletion of immature post-positive selection thymocytes. In contrast, CCR7 is expressed several days later and is required for medullary localization and negative selection of mature thymocytes. In addition, CCR4 and CCR7 differentially enforce self-tolerance, with CCR4 enforcing tolerance to self-antigens presented by activated APCs, which express CCR4 ligands. Our findings show that CCR7 expression is not synonymous with medullary localization and support a revised model of central tolerance in which CCR4 and CCR7 promote early and late stages of negative selection, respectively, via interactions with distinct APC subsets.

Literature review pengaruh pemberian bekam basah terhadap hipertensi

Background: Hypertension is a non-communicable disease that is one of the causes of mortality in the world. Hypertension is often referred to as the silent killer because it is often without complaints, a serious condition that significantly increases the risk of heart, brain, kidney, and other diseases. Complications in patients with hypertension can be life-threatening, so pharmacological therapies is wet cupping which can lower blood pressure. Objective: The purpose of this study was to determine the effect of wet cupping on hypertension. Methods: The method in this study uses the PICOS framework, namely Population/problem (P), Intervention (I), Comparison (C), Outcome (O), and study design (S). In the early stages of article selection, 68 articles were found from the PubMed database, ProQuest, Elsevier, Science Direct, Google Scholar, Harvard Library, Springer Link, ResearchGate, Taylor and Francis search engine after further matching, 60 article were selected, then 53 articles were excluded because they were not appropritate. With problems, intervention, study design, and no effect of wet cupping on hypertension so that 7 articles were obtained that matched the research topic. Results: The results of the search that wet cupping affects hypertension, this is evidenced from before and after the wet cupping intervention was given, namely there was a significant decrease in systole and diastole with p-value <0,05. This wet cupping treatment is more effective, given once a month for satisfactory results with an adminstration time of 15-30 minutes.Conclusion: This treatment is highly recommended to the general public, health clinics, hospitals, and health workers and this treatment provides many benefits.

A spatial-based approach applied to early selection stages in a forage breeding program

A spatial-based approach applied to early selection stages in a forage breeding program

Training set designs for prediction of yield and moisture of maize test cross hybrids with unreplicated trials.

Unreplicated field trials and genomic prediction are both used to enhance the efficiency in early selection stages of a hybrid maize breeding program. No results are available on the optimal experimental design when combining both approaches. Our objectives were to investigate the effect of the training set design on the accuracy of genomic prediction in unreplicated maize test crosses. We carried out a cross validation study on basis of an experimental data set consisting of 1436 hybrids evaluated for yield and moisture for which genotyping information of 461 SNP markers were available. Training set designs of different size, implementing within environment prediction, within year prediction, across year prediction, and combinations of data sources across years and environments were compared with respect to their prediction accuracy. Across year prediction did not reach prediction accuracies that are useful for genomic selection. Within year prediction across environments provided useful correlations between observed and predicted breeding values. The prediction accuracies did not improve when adding to the training set data from previous years. We conclude that using all data available from unreplicated tests of the current breeding cycle provides a good accuracy of predicting test crosses, whereas adding data from previous breeding cycles, in which the genotypes are less related to the tested material, has only limited value for increasing the prediction accuracy.

The Role of the Angular Gyrus in Goal-directed Behavior-Two Transcranial Magnetic Stimulation Studies Examining Response Outcome Learning and Outcome Anticipation.

Learning the contingencies between a situational context (S), one's own responses (R), and their outcomes (O) and selecting responses according to their anticipated outcomes is the basis of a goal-directed behavior. Previous imaging studies found the angular gyrus (AG) to be correlated to both the representation of R-O associations and outcome-based response selection. Based on this correlational relationship, we investigated the causal link between AG function and goal-directed behavior in offline and online TMS experiments. To this end, we employed an experimental R-O compatibility paradigm testing outcome anticipation during response selection and S-R-O knowledge to probe S-R-O learning. In Experiment 1, we applied 1-Hz rTMS offline to the AG or the vertex before participants performed the experimental tasks. In Experiment 2, we applied online 10-Hz pulse trains to the AG or used sham stimulation during an early action selection stage in half of the trials. In both experiments, the R-O compatibility effect was unaltered when response selection was outcome-based, suggesting no causal role of the AG in outcome anticipation during response selection. However, in both experiments, groups with AG stimulation showed significantly modulated knowledge of S-R-O associations in a posttest. Additionally, in an explorative analysis, we found an induced R-O compatibility effect later in the experiment when response selection was guided by stimulus-response rules, suggesting reduced selectivity of outcome anticipation. We discuss possible compensatory behavioral and brain mechanism as well as specific TMS-related methodical considerations demonstrating important implications for further studies investigating cognitive function by means of TMS.

SWATH-based quantitative proteomics gives insight into a significant proteome shift in UCB-1 pistachio rootstock under salt stress

The hybrid pistachio rootstock UCB-1 is especially tolerant to abiotic stresses including salinity. However, the effect of salinity on its whole proteome is obscure, which limits our ability to develop high-confident protein biomarkers to facilitate the genetic improvement of pistachio cultivars with enhanced salt-tolerance. In the present study, using sequential window acquisition of all theoretical mass spectra (SWATH-MS) technology, the expression of 1150 proteins was quantified, with the expression of 459 proteins significantly impacted by salt exposure. Functional enrichment determined that salinity stress increases the level of proteins associated with response to salt stress and reduces the level of ribosomal subunits. We used KEGG pathway enrichment analysis to link salt exposure with a set of alterations in several pathways including ribosome components, biosynthesis of phenylpropanoids, and carbon fixation in photosynthetic organisms. To track the potential consequences of protein alteration with more crucial biological functions in the enriched pathways, the incorporation of SWATH-MS data and protein-protein interactions (PPIs) analysis were then conducted. Centrality analysis of the three top-ranked enriched pathways highlighted that any alterations in the abundance of EMB3010, PA2, NAD-ME1, and PGAM proteins may result in desirable consequences on salt tolerance in UCB-1. Therefore, centrality analysis of whole proteome survey-derived responsive PPIs was useful in the identification, prioritization, and selection of potential biomarker candidates by which the need for expensive and time-consuming experimental validation will be minimized. At the early selection stages in tree breeding programs, integration of proteomics-generated resources with conventional programs can assist in developing fast selection assays.

Effects of Water Deficit Stress on Growth Parameters of Robinia pseudoacacia L. Selected Clones under In Vitro Conditions

Rapid screening methods for drought-resistant genotypes are urgently needed in tree improvement programs in the face of current climate change. We used a plant tissue culture technique to assess the phenotypic response of three highly productive genotypes of Robinia pseudoacacia to water deficit induced by mannitol and sucrose in a range of water potentials from 0 MPa to −1.5 MPa in an eight-week experiment. Our study showed genotype-specific responses to induced drought stress, indicating the potential for tree improvement in productivity and stress tolerance. Considering that all plantlets were constantly supplied with carbon, from the medium during the drought-induced experiment, our results suggest that hydraulic failure rather than carbon starvation may be the main cause of drought-induced mortality. Furthermore, our results showed different metabolic pathways of sucrose depending on the concentration of sucrose in the medium and different responses to osmoticum (mannitol vs. sucrose) and its concentration among the clones tested. We believe, that for large-scale breeding programs wanting to select for drought-tolerant genotypes, the use of culture media containing 90 gL−1 mannitol or 90 gL−1 sucrose at an early selection stage should provide satisfactory screening results. However, lab-based screening should be supported by further field trials, preferably at multiple sites, to assess the long-term impact and phenotypic stability of the early selection strategies.