Early Morning Period Research Articles

Longitudinal association between saliva and hair cortisol concentration: A systematic comparison

Cortisol assays from hair have become increasingly common in psychoneuroendocrinological research as indicators of long-term output relevant to stress and health outcomes. Comparisons of hair cortisol concentration (HCC) with salivary samples have produced mixed findings, and it remains unclear which aspects of the diurnal salivary profile correspond most closely to HCC, and what time intervals between saliva and hair sampling are most relevant, taking the rate of hair growth into account. This longitudinal study aimed to evaluate the correspondence between HCC and parameters of total salivary cortisol output in the morning (CARauc and CARi) and during the rest of the day excluding the early morning period (DAYauc), by systematically studying three time periods – two weeks, four weeks, and six weeks – before hair sampling. At each time period, 54 female university students (mean age: 20.85 ± 1.16 years) provided three saliva cortisol samples on day 1 at 11 am, 3 pm, at bedtime, then two samples the following day on waking and 30 min after awakening. Hair strand collection (1 cm nearest the scalp) took place two weeks after the last saliva sample. Results of multivariable regressions indicate that HCC was consistently associated with DAYauc for all three time periods and with the aggregate DAYauc across days after adjusting for age, body mass index, smoking, oral contraceptive use, hair washing frequency and hair treatments. The strongest associations were found for DAYauc two weeks before hair sampling (β = 0.578, p < 0.001) and the aggregated DAYauc across all three time periods (β = 0.596, p < 0.001), although the confidence intervals overlapped those for four and six week analyses. There was no significant association between HCC and either CARauc or CARi. Our study confirms that hair cortisol could be a reliable retrospective biomarker of basal and long-term cortisol output secretion at least up to six weeks earlier. The results contribute to a better understanding of the different associations between HCC and salivary cortisol in the morning and the rest of the day, while also having implications for the use of HCC as an outcome measure in intervention and treatment research.

OR03-5 Identification of Four New Glucotypes during the First Year after Type 1 Diabetes Onset Using Continuous Glucose Monitoring Metrics

Abstract Current markers of β-cell function poorly reflect glucose homeostasis after diabetes onset, mainly because of the lack of integration of parameters of insulin sensitivity and β-cell responsiveness to glucose. Recently, the widespread use of continuous glucose monitoring (CGM) helped stratify glucose control in patients with long-term diabetes in new clinically relevant glucotypes. In our DIATAG study, we investigated how CGM metrics may help to segregate patients with new-onset type 1 diabetes (T1D) entering or not in partial remission (PR). We collected data from 66 pediatric patients with T1D (10.4±4.7 yo) during the first year after diabetes onset (Δ). Clinical parameters (i.e. A1C, insulin daily dose, insulin-dose adjusted A1c [IDAA1C]) and CGM data were collected at Δ+3, +6, +9, +12 months (n=168, 57% remitters [IDAA1C &amp;lt;9]). A panel of 46 CGM metrics was calculated on an hourly and daily basis using iglu package (1). Using unsupervised hierarchical clustering based on daily CGM metrics and clinical parameters, we identified four clusters of glucose metrics that differed from each other (p&amp;lt;0.05). Cluster 1 was characterized by an increased glucose stability (coefficient of variation [CV] 32±5%) within time in target (TIT [63-140 mg/dL], 83±6%) with 9% of sensor values in 63-70 mg/dL range in the early morning period. Cluster 2 exhibited a progressive decrease of TIT (60±14%) and an increase of target above range (TAR [&amp;gt;180 mg/dL], 20±9%) especially during the day while target in range (TIR [70-180 mg/dL]) was nearly equivalent to Cluster 1 (80±8%). Notably, CV remained low (32±5%) with little time spent below range (TBR [&amp;lt;70 mg/dL], 5±2%). Cluster 3 demonstrated a net increase of CV during all the nycthemere (46±7%) concomitantly to an increase of TBR (14±7%). Interestingly, TIT and TAR remained close to values from Cluster 2 (55±11%; 23±10%). Finally, Cluster 4 demonstrated major hyperglycemias (TAR, 55±13%) with increased CV (47±9%) and decreased TBR (8±6%). Interestingly, clustering allowed clinically relevant segregation of intermediate values of IDAA1C (i.e. 7.5-10) between Cluster 2 and Cluster 3, independently of the remission status. Moreover, participants at Δ+3 months distributed across all clusters (with respectively 38% in Cluster 1, 26% in Cluster 2, 29% in Cluster 3 and 7% in Cluster 4) highlighting the usefulness of CGM in characterizing disease heterogeneity from diabetes onset. In our study, a combination of CGM metrics and clinical parameters unraveled key clinical milestones of glucose homeostasis and remission status during the first year of T1D. 1. Broll et al, 2021;16(4): e0248560. Presentation: Saturday, June 11, 2022 12:30 p.m. - 12:45 p.m.

662-P: Glycemic Variability Patterns Strongly Correlate with Partial Remission Status in Children with Newly Diagnosed Type 1 Diabetes

Residual β-cell secretion is commonly used as primary endpoint of β-cell mass prevention trials. Recently, peak C-peptide was challenged as a clinically relevant marker of β-cell function considering its poor integration of insulin sensitivity and β-cell glucose responsiveness. In our DIATAG study, we evaluated the correlations between metrics of glycemic variability (GV) and markers of diabetes control (i.e. A1C, insulin daily dose, insulin dose-adjusted A1C [IDAA1C]) to identify whether GV indexes may overcome residual β-cell secretion estimates (BSE) in the evaluation of glucose homeostasis during the first year of type 1 diabetes (e.g. remission period) . Data from 78 new-onset type 1 diabetes pediatric patients were collected from diabetes onset (Δ) and cross-sectionally compared using adjusted mixed-effects models at Δ+3, +12 months for BSE (n=119) and Δ+3, +6, +9, +12 months for glucose control and CGM metrics (n=169) . BSE demonstrated weak-to-moderate correlations with clinical parameters and CGM metrics (r2= 0.05-0.25; p&amp;lt;0.05) . CGM metrics strongly correlated with clinical parameters (r2 &amp;gt;0.52; p&amp;lt;0.05) and were sufficient to distinguish remitters from non-remitters (i.e. IDAA1C respectively &amp;lt;9 or ≥9) . Moreover, CGM metrics from remitters exhibited specific nycthemeral patterns characterized by increased glycemic stability (in 63-160 mg/dL range) across days in the early morning period and decreased grade II hypoglycemia during the day (p&amp;lt;0.01) . Using hierarchical clustering on 46 CGM metrics and clinical parameters (n=169) , we identified four unique glucotypes (p&amp;lt;0.001) during the first year of diabetes that allowed better segregation of intermediate values of IDAA1C from Δ+3 months (38% in Group 1, 26% in Group 2, 29% in Group 3, 7% in Group 4) . We conclude that a combination of CGM metrics and clinical parameters unraveled key clinical milestones of glucose homeostasis and remission status during the first year of type 1 diabetes. Disclosure O.G. Polle: None. A.A. Delfosse: None. M. Martin: None. I. Gies: None. M. Iebrethon: None. J. Louis: None. N. Seret: None. L. Gatto: None. Funding Fond National de la Recherche Scientifique (FRIA grant, FSR starting grant) Belgian Society for Pediatric Endocrinology and Diabetology (BESPEED) Société Francophone du Diabète (SFD)

Interaction Between Equatorial to Low‐Latitude Postmidnight F‐Region Irregularities and LSTIDs in China During Geomagnetic Disturbances Based on Ground‐Based Instruments

AbstractIn this paper, the interaction between equatorial to low‐latitude postmidnight ionospheric irregularities and large‐scale traveling ionospheric disturbances (LSTIDs) on 14 December 2015 during strong geomagnetic disturbances in China is studied by using multiple ground‐based observations. Postmidnight irregularities initiated near the magnetic equator and extended northward to higher latitudes (∼28°N) until the local early morning period (∼05 LT), which is unusual in the Northern Hemisphere winter. Multibeam observations by the very high frequency coherent scatter radar at Fuke (19.5°N, 109.1°E; dip latitude 14.4°N), Hainan Island, China, showed that the meter‐scale field‐aligned irregularities were still in the evolutionary phase during the postmidnight period (∼04 LT) instead of the fossil structures that must already be in their decay phase. Meanwhile, the emergence of the postmidnight ionospheric irregularities was accompanied by the passage of LSTIDs, as revealed by total electron content measurements. Southward and northward LSTIDs, with velocities of ∼550–570 m/s and periods of ∼80–100 min, that originated from opposite hemispheres met at ∼27°–28°N, and the LSTIDs might produce localized perturbation during their passage to initiate the instability for the postmidnight irregularity development. The height of the ionospheric F‐layer measured by the collocated Digisonde at Fuke was suggested to be modulated by the overshielding penetration electric field and storm‐related atmospheric gravity waves (AGWs). This interesting case gives insight into the important role of storm‐related AGWs in providing seeding sources for plasma bubble development and reveals unique characteristics of coupling between LSTIDs and plasma bubbles during the postmidnight sector.

Using Wearable Cameras to Categorize the Type and Context of Screen-Based Behaviors Among Adolescents: Observational Study.

BackgroundAutomated wearable cameras present a new opportunity to accurately assess human behavior. However, this technology is seldom used in the study of adolescent’s screen exposure, and the field is reliant on poor-quality self-report data.ObjectiveThis study aimed to examine adolescents’ screen exposure by categorizing the type and context of behaviors using automated wearable cameras.MethodsAdolescents (mean age 15.4 years, SD 1.6 years; n=10) wore a camera for 3 school evenings and 1 weekend day. The camera captured an image every 10 seconds. Fieldwork was completed between February and March 2020, and data were analyzed in August 2020. Images were date and time stamped, and coded for screen type, content, and context.ResultsData representing 71,396 images were analyzed. Overall, 74.0% (52,842/71,396) of images contained screens and 16.8% (11,976/71,396) of images contained multiple screens. Most screen exposures involved television sets (25,950/71,396, 36.3%), smartphones (20,851/71,396, 29.2%), and laptop computers (15,309/71,396, 21.4%). The context of screen use differed by device type, although most screen exposures occurred at home (62,455/64,856, 96.3%) and with solitary engagement (54,430/64,856, 83.9%). The immediate after-school period saw high laptop computer use (4785/15,950, 30.0%), while smartphone use (2059/5320, 38.7%) peaked during prebedtime hours. Weekend screen exposure was high, with smartphone use (1070/1927, 55.5%) peaking in the early morning period and fluctuating throughout the day.ConclusionsThere was evidence for high screen use during the after-school and weekend period, mostly through solitary engagement, and within the home environment. The findings may inform the basis of larger studies aimed at examining screen exposure in free-living conditions.

Diurnal variations of rainfall affected by complex topography based on high-density observation in Chongqing over southwest China

Located in the eastern edge of the Sichuan Basin (SCB) in the southwest China, Chongqing is a mountainous region with typical complex topographic features. Using the hourly rainfall observation data of high-density 1686 meteorological stations in Chongqing during warm season from 2009 to 2016, we investigated the diurnal characteristics of precipitation affected by complex topography. The complex mountainous terrain has a significant impact on diurnal variations and distinct regional features of rainfall amount, frequency, and intensity. The stations located in the higher complex mountainous areas have greater rainfall amount, frequency, and intensity than those in the lower surrounding areas. In addition, the detailed characteristics of the rainfall amount and frequency in the four study regions further show that the rainfall amount and frequency significantly increase with the rise of elevation, especially in the area that terrain height sharply increases along the mountain extending direction. The diurnal variation of the rainfall amount is characterized by a bimodal structure with a dominant early-morning peak occurring at approximately 0700 LST (23 UTC) and a weaker secondary late-afternoon peak at approximately 1600 LST (08 UTC), while the rainfall frequency has a single early-morning peak. The terrain height has a significant impact on the proportions of the early-morning rainfall. With the elevation increasing in the four study regions, the proportions of rainfall amount (frequency) that occurs during early-morning period decrease.

Sex-Dependent Association Between Early Morning Ambulatory Blood Pressure Variations and Acute Mountain Sickness.

BackgroundAcute high altitude (HA) exposure elicits blood pressure (BP) responses in most subjects, and some of them suffer from acute mountain sickness (AMS). However, a 24-h ambulatory BP (ABP) change and the correlation with the occurrence of AMS in different sexes are still unclear.ObjectivesThis prospective study aimed to investigate HA induced BP responses in males and females and the relationship between AMS and 24-h ABP.MethodsForty-six subjects were matched according to demographic parameters by propensity score matching with a ratio of 1:1. All the subjects were monitored by a 24-h ABP device; the measurement was one period of 24 h BP. 2018 Lake Louise questionnaire was used to evaluate AMS.ResultsBoth the incidence of AMS (14 [60.9%] vs. 5 [21.7%], P = 0.007) and headache (18 [78.3%] vs. 8 [34.8%], P = 0.003) were higher in females than in males. All subjects showed an elevated BP in the early morning [morning systolic BP (SBP), 114.72 ± 13.57 vs. 120.67 ± 11.10, P = 0.013]. The elevation of morning SBP variation was more significant in females than in males (11.95 ± 13.19 vs. −0.05 ± 14.49, P = 0.005), and a higher morning BP surge increase (4.69 ± 18.09 vs. −9.66 ± 16.96, P = 0.005) was observed after acute HA exposure in the female group. The increase of morning SBP was associated with AMS occurrence (R = 0.662, P < 0.001) and AMS score (R = 0.664, P = 0.001). Among the AMS symptoms, we further revealed that the incidence (R = 0.786, P < 0.001) and the severity of headache (R = 0.864, P < 0.001) are closely correlated to morning SBP.ConclusionsOur study demonstrates that females are more likely to suffer from AMS than males. AMS is closely associated with elevated BP in the early morning period, which may be correlated to higher headache incidence in subjects with higher morning SBP.

Comprehensive assessment of the indoor air quality in a chlorinated Olympic-size swimming pool

Elite swimmers and swimming pool employees are likely to be at greater health risk due to their regular and intense exposure to air stressors in the indoor swimming pool environment. Since data on the real long-term exposure is limited, a long-term monitoring and sampling plan (22 non-consecutive days, from March to July 2017) was carried out in an indoor Olympic-size pool with a chlorine-based disinfection method to characterize indoor environments to which people involved in elite swimming and maintenance staff may be exposed to. A comprehensive set of parameters related with comfort and environmental conditions (temperature, relative humidity (RH), carbon dioxide (CO2) and monoxide and ultrafine particles (UFP)) were monitored both indoors and outdoors in order to determine indoor-to-outdoor (I/O) ratios. Additionally, an analysis of volatile organic compounds (VOC) concentration and its dynamics was implemented in three 1-hr periods: early morning, evening elite swimmers training session and late evening. Samplings were simultaneously carried out in the air layer above the water surface and in the air surrounding the pool, selected to be representative of swimmers and coaches/employees’ breathing zones, respectively. The results of this work showed that the indoor climate was very stable in terms of air temperature, RH and CO2. In terms of the other measured parameters, mean indoor UFP number concentrations (5158 pt/cm3) were about 50% of those measured outdoors whereas chloroform was the predominant substance detected in all samples collected indoors (13.0–369.3 µg/m3), among a varied list of chemical compounds. An I/O non-trihalomethanes (THM) VOC concentration ratio of 2.7 was also found, suggesting that, beyond THM, other potentially hazardous VOC have also their source(s) indoors. THM and non-THM VOC concentration were found to increase consistently during the evening training session and exhibited a significant seasonal pattern. Compared to their coaches, elite swimmers seemed to be exposed via inhalation to significantly higher total THM levels, but to similar concentrations of non-THM VOC, during routine training activities. Regarding swimming employees, the exposure to THM and other VOC appeared to be significantly minimized during the early morning period. The air/water temperature ratio and RH were identified as important parameters that are likely to trigger the transfer processes of volatile substances from water to air and of their accumulation in the indoor environment of the swimming pool, respectively.

Working-hour phenomenon in obstetrics is an attainable target to improve neonatal outcomes

Giving birth in a health care facility does not guarantee high-quality care or favorable outcomes. The working-hour phenomenon describes adverse outcomes of institutional births outside regular working hours. The objectives of the study were to evaluate whether the time of birth is associated with adverse neonatal outcomes and to identify the riskiest time periods for obstetrical care. This nationwide retrospective cohort study analyzed data from 2008 to 2016 from all 82 obstetric departments in Austria. Births at ≥ 23+0 gestational weeks with ≥500 g birthweight were included. Independent variables were categorized by the time of day vs night as core time (morning, day) and off hours (evening, nighttime periods 1-4). The composite primary outcome was adverse neonatal outcome, defined as arterial umbilical cord blood pH <7.2, 5 minute Apgar score <7, and/or admission to the neonatal intensive care unit. Multivariate logistic regression was used to develop a model to predict these adverse neonatal outcomes. Of 462,947 births, 227,672 (49.2%) occurred during off hours and had a comparable distribution in all maternity units, regardless of volume (<500 births per year: 50.3% during core time vs 49.7% during off hours; ≥500 births per year: 50.7% core time vs 49.3% off hours; perinatal tertiary center: 51.2% core time vs 48.8% off hours). Furthermore, most women (35.8-35.9%) gave birth between 2:00 and 5:59 am (night periods 3 and 4). After adjustment for covariates, we found that adverse neonatal outcomes also occurred more frequently during these night periods 3 and 4, in addition to the early morning period (night 3: odds ratio, 1.05; 95% confidence interval, 1.03-1.08; P < .001; night 4: odds ratio, 1.08; 95% confidence interval, 1.05-1.10; P < .001; early morning period: odds ratio, 1.05; 95% confidence interval, 1.02-1.08; P < .001). The adjusted odds for adverse outcomes were lowest for births between 6:00 and 7:59 pm (odds ratio, 0.96; 95% confidence interval, 0.93-0.99; P= .006). There is an increased risk of adverse neonatal outcomes when giving birth between 2:00 and 7:59 am. The so-called working-hour phenomenon is an attainable target to improve neonatal outcomes. Health care providers should ensure an optimal organizational framework during this time period.

Sleep disorders, nocturnal blood pressure, and cardiovascular risk: A translational perspective.

Cardiovascular disease (CVD) represents the first cause of death globally. The nighttime is generally a period of relative protection from CVD events such as myocardial infarction, sudden cardiac death, and stroke, at least compared to the early morning period. The nighttime also generally entails lower values of arterial blood pressure (ABP) and heart rate (HR) and higher cardiac parasympathetic modulation. These day-night cardiovascular rhythms are ultimately driven by circadian molecular oscillators in the hypothalamic suprachiasmatic nucleus and in peripheral cells, including those in the heart, blood vessels, and kidneys. The wake-sleep states are intermediate mechanisms of circadian cardiovascular regulation, with non-REM sleep decreasing ABP and HR and increasing cardiac parasympathetic modulation at the beginning of the night. Obstructive sleep apnea, insomnia, and the restless legs syndrome have high prevalence in the general population and may increase nighttime cardiovascular activity and CVD risk. CVD risk is better predicted by ABP values during nighttime sleep than during daytime wakefulness. Higher nighttime values of ABP and HR increase cardiac work and vessel wall stress. During the night, circadian rhythms may enhance cardiac responses to hypertrophic stimuli, increase vascular smooth muscle Rho kinase activity and contractility, decrease endothelial nitric oxide production and vascular responses to vasodilators, and increase circulating monocytes with the potential to infiltrate atherosclerotic plaques. Together, these factors configure a "perfect storm" scenario that may make increased cardiovascular activity during the night a final common mechanism linking sleep disorders to CVD risk.

Abstract TMP61: Enrolling Stroke Patients in Prehospital Research During Late Night and Early Morning Times

Background: Enrolling stroke patients into clinical research studies often necessitates having investigators available to field calls (from paramedics, potential subjects and ED personnel) as well as study nurses available at enrolment sites. Enrolment of patients overnight may thus not be cost effective if there are low numbers. Late night enrolment may have different characteristics such as more severe stroke or greater proportion of intracerebral hemorrhage. Methods: The Field Administration of Stroke Therapy-Magnesium (FAST-MAG) clinical trial enrolled patients 24 hours per day with designated on-call enrolling investigators and study nurses. We compared enrolment in the late night and early morning period defined as 10:00PM through 5:59AM compared to standard hours of 6:00AM through 9:59PM. We also considered implications of changing the late night/early morning definition to Midnight and 8:00AM. Results: Of 1700 enrolments, 171 (10%) occurred between 10PM-6AM. There was no significant differences in the late-night enrolments in stroke subtype (27% vs 22% ICH, p=0.268), age, ethnicity, race, vascular risk factors, prehospital stroke severity (LAMS) or hospital arrival stroke severity (NIHSS). Late night enrolments were less likely to be women (35% v 44%, p=0.035). There were 61 enrolments (3.4%) between midnight to 5:59AM. Expanding the early morning period from 6AM to 8AM would lead to 76 (4.4%) less enrollments. Conclusions: Late night/early morning enrolments are not clinically or demographically different from those during regular hours and accounted for 10% of overall enrolments. Less than 4% of enrolments occurred between midnight and 6:00AM. Future prehospital stroke trials can consider deferring enrolment in the late night/early morning period to help with resource allocation.

Diurnal Variations of Summer Precipitation over the Qilian Mountains in Northwest China

Based on the high-density hourly rain-gauge data from 265 stations over the Qilian Mountains in Northwest China, climatic mean diurnal variations of summer rainfall over different topographies of this area are investigated. Influences of the gauge elevations on the diurnal variation of rainfall are also revealed. Distinct regional features of diur-nal variations in rainfall are observed over the Qilian Mountains. Rainfall over the Qinghai Lake areas shows a single nocturnal peak. A dominant, late-afternoon peak of rainfall occurs over the mountain tops. Over the northeastern and southeastern slopes, a dominant diurnal peak appears in the late afternoon, and an evident second peak is found in the early morning, respectively. The strengths of the early-morning peaks in the rainfall frequency are closely related to the rainfall events with different durations over the two slopes. The early-morning peak is dominant across plains with low elevations. From the mountain tops to the plains, the diurnal peaks of rainfall gradually vary from the domi-nant late-afternoon peak to the dominant early-morning peak with the enhanced early-morning peak in concurrent with the decreasing gauge elevation over the northeastern and southeastern slopes. Further examination indicates that the rainfall at higher elevations over the northeastern and southeastern slopes occurs more readily in the afternoon, compared to the lower elevations. This phenomenon corresponds to the result that the proportion of the rainfall frequency occurring during the early-morning period decreases with increasing elevations over the two slopes.

Chronomodulation of cyclin-dependent kinases 4/6 inhibitor may reduce hematological toxicities? A review of literature

&lt;p class="s3"&gt;Nucleotide excision repair, DNA damage checkpoints, and apoptosis are under the influence of the circadian rhythm1. Circadian rhythm is defined as oscillations in the behavior and biochemical changes in an individual that repeats itself after the span of 24 h approximately. Cyclin-dependent kinase (CDK) inhibition causes cell cycle arrest and subsequent circadian stage-dependent gating of cells at G2-M interface of the cell cycle. Few anecdotes have suggested that chronomodulation reduces hematological toxicity in cell cycle-specific chemotherapy, especially S1-specific chemotherapy. In a study conducted by Boucher et al., 2016, circadian rhythm plays a role in the regulation of human mesenchymal stem cells (hMSCs) differentiation and division and likely represents key factor in maintaining hMSCs properties. If we apply the knowledge of circadian clock, then we know the fact that bone marrow stem cells (BMSCs) are under the control of circadian rhythm and G1-S phase of cell division cycle occurs at the early morning period of solar day. If CDK4/6 plasma peak level coincides with G1-S phase of BMSCs, then theoretically cytopenia may occur, which again is the sign of CDK4/6 action but is also the reason of its toxicity. Chronomodulation studies of CDK4/6 inhibitor may reduce hematological toxicity of CDK4/6 inhibitor.&lt;/p&gt;

Comparison of the human-baited double net trap with the human landing catch for Aedes albopictus monitoring in Shanghai, China

BackgroundHuman landing catch (HLC) is the most efficient method for Aedes monitoring, but it is not ethical due to its high risk of human exposure to pathogens. We designed trials to assess the performance of an alternative human-baited double net trap (HDN) for field Aedes albopictus monitoring compared with the standard HLC.MethodsOutdoor HDN and HLC catches were conducted simultaneously at 15 field sites on two sunny days in mid-July and August. The tests were performed 3 h apart: an early morning period (7:30–8:30 h), a pre-sunset period (16:30–17:30 h) and a post-sunset period (18:30–19:30 h). A total of 90 comparisons were made between the two methods. Field comparisons were designed to minimize half-hour bias and human-bait attraction bias.ResultsTwo mosquito species were collected by HDN and HLC, with the predominated species being Ae. albopictus (HDN: n = 1325, 97.35% of total; HLC: n = 531, 92.51% of total). A small proportion were adults of the Culex pipiens complex (HDN: n = 36, 2.65% of total; HLC: n = 43, 7.49% of total). Although the mean Ae. albopictus catch per hour of HLC was significantly higher than HDN (14.72 vs 5.90 per h, t(178) = 3.151, P = 0.003), there were significant positive spatial and temporal correlations between HLC and HDN for Ae. albopictus sampling among different sites and hours (r(90) = 0.785, P < 0.001; r(90) = 0.785, P < 0.001). Both methods proved that Ae. albopictus was most active during the hours before sunset and least active after sunset. No significant variation was observed in Ae. albopictus catch size of HDN between groups of more attractive and less attractive humans (3.38 vs 2.51 per 30 min, t(88) = 1.283, P = 0.201).ConclusionsWith moderate sampling efficiency, significantly positive spatial correlation with HLC, and less human-bait attraction bias, HDN appears to be a safer alternative to HLC for Ae. albopictus monitoring in Shanghai. With mosquito activity peaking in the pre-sunset hours, Ae. albopictus catches of HDN should be performed in the hours before dark. The trap design could be improved to make it more portable and easier for field operation.

The effect of concentrate supplementation on milk production and cow traffic in early and late lactation in a pasture-based automatic milking system

The objective of this experiment was to establish the effect of low-concentrate (LC) and high-concentrate (HC) supplementation in the early and late periods of lactation on milk production and cow traffic in a pasture-based automatic milking (AM) system. In total, 40 cows (10 primiparous and 30 multiparous) were randomly assigned to one of the two treatments. The experimental periods for the early and late lactation trials extended from 23 February to 12 April 2015 and 31 August to 18 October 2015, respectively (49 days in each trial period). The early lactation supplement levels were 2.3 and 4.4 kg/cow per day for LC and HC, respectively, whereas the late lactation supplement levels were 0.5 and 2.7 kg/cow per day for LC and HC, respectively. Variables measured included milking frequency, milking interval, milking outcome and milking characteristics, milk yield/visit and per day, wait time/visit and per day, return time/visit and the distribution of gate passes. As the herd was seasonal (spring) calving, the experimental periods could not run concurrently and as a result no statistical comparison between the periods was conducted. There was no significant effect of treatment in the early lactation period on any of the milk production, milking characteristics or cow traffic variables. However, treatment did significantly affect the distribution of gate passes, with the HC cows recording significantly more gate passes in the hours preceding the gate time change such as hours 7 (P<0.01), 15 (P<0.05), 20, 21 (P<0.001), and 22 (P<0.05), whereas the LC treatment recorded significantly more gate passes in the hours succeeding the gate time change, such as time points 2 (P<0.01) and 10 (P<0.05). There was a significant effect of treatment in late lactation, with HC having a greater milk yield (P<0.01), milking duration and activity/day (P<0.05), while also having a significantly shorter milking interval (P<0.05) and return time/visit (P<0.01). The distribution of gate passes were similar to the early lactation period, with HC also recording a significantly greater number of gate passes during the early morning period (P<0.01) when visitations were at their lowest. Any decision regarding the supplementing of dairy cows with concentrates needs to be examined from an economic perspective, to establish if the milk production and cow traffic benefits displayed in late lactation outweigh the cost of the concentrate; thereby ensuring that the decision to supplement is financially prudent.

Relationship between Alcohol Purchasing Time and Alcohol Use Disorder in South Korea.

ObjectivesCurrently, time of alcohol purchase is not part of the policies to regulate alcohol consumption in South Korea. This study was conducted to determine the relationship between alcohol purchasing time and alcohol use disorder.MethodsThe survey for this study was conducted in geographically diverse regions of South Korea in 2012. Respondents’ purchasing behaviors for both on-licensed (i.e., allows for consumption within the premises) and off-licensed (i.e., where alcohol is consumed off the premises) outlets and time of alcohol consumption were collected. Alcohol consumption patterns were examined using the Rapid Alcohol Problem Screen 4 (RAPS4). Data were also analyzed by age, gender and purchasing time.ResultsResults showed that among the off-licensed premises, supermarkets appear to be the most popular venue while for on-licensed premises; alcohol was generally consumed inside hotels/pubs regardless of age and gender of the purchaser. Purchasing of alcohol was highest during the day and early evening period (9:00 a.m. to 9:59 p.m.). Females are most likely to abuse alcohol than males during the early morning period and is that period after 12:00 midnight.ConclusionAnalysis suggests that the survey instrument used in the International Alcohol Control Study is being used to collect data on alcohol purchasing time consumption; therefore, the potential is there to provide accurate results to contribute appropriate policy responses to reduce alcohol related-harm.

Daily and seasonal trends of electricity and water use on pasture-based automatic milking dairy farms

The objective of this study was to identify the major electricity and water-consuming components of a pasture-based automatic milking (AM) system and to establish the daily and seasonal consumption trends. Electricity and water meters were installed on 7 seasonal calving pasture-based AM farms across Ireland. Electricity-consuming processes and equipment that were metered for consumption included milk cooling components, air compressors, AM unit(s), auxiliary water heaters, water pumps, lights, sockets, automatic manure scrapers, and so on. On-farm direct water-consuming processes and equipment were metered and included AM unit(s), auxiliary water heaters, tubular coolers, wash-down water pumps, livestock drinking water supply, and miscellaneous water taps. Data were collected and analyzed for the 12-mo period of 2015. The average AM farm examined had 114 cows, milking with 1.85 robots, performing a total of 105 milkings/AM unit per day. Total electricity consumption and costs were 62.6 Wh/L of milk produced and 0.91 cents/L, respectively. Milking (vacuum and milk pumping, within-AM unit water heating) had the largest electrical consumption at 33%, followed by air compressing (26%), milk cooling (18%), auxiliary water heating (8%), water pumping (4%), and other electricity-consuming processes (11%). Electricity costs followed a similar trend to that of consumption, with the milking process and water pumping accounting for the highest and lowest cost, respectively. The pattern of daily electricity consumption was similar across the lactation periods, with peak consumption occurring at 0100, 0800, and between 1300 and 1600 h. The trends in seasonal electricity consumption followed the seasonal milk production curve. Total water consumption was 3.7 L of water/L of milk produced. Water consumption associated with the dairy herd at the milking shed represented 42% of total water consumed on the farm. Daily water consumption trends indicated consumption to be lowest in the early morning period (0300-0600 h), followed by spikes in consumption between 1100 and 1400 h. Seasonal water trends followed the seasonal milk production curve, except for the month of May, when water consumption was reduced due to above-average rainfall. This study provides a useful insight into the consumption of electricity and water on a pasture-based AM farms, while also facilitating the development of future strategies and technologies likely to increase the sustainability of AM systems.

Early Morning Functional Impairments in Stimulant-Treated Children with Attention-Deficit/Hyperactivity Disorder Versus Controls: Impact on the Family.

Objective: Children with attention-deficit/hyperactivity disorder (ADHD) frequently manifest early morning functional (EMF) impairments before school. We conducted a quantitative research survey to assess the impact of these EMF impairments on the family unit (caregiver, spouse/partner, and siblings).Study Design: We developed an online survey questionnaire to collect data from 300 primary caregivers of children with ADHD and 50 primary caregivers of children who did not have ADHD.Results: Although the ADHD children we surveyed were currently treated with stable doses of stimulants as their primary ADHD medication for at least 3 months, their parents reported high levels of EMF impairments in the child, which had a substantial negative effect on the emotional well-being of parents, on parents' functioning during the early morning routine, and on the level of conflict with siblings. The impact of EMF impairments on family functioning was mediated by the severity of the index child's impairments.Conclusions: EMF impairments exert a pervasive and significantly negative emotional and functional burden on not only the primary caregiver but also on the spouse/partner and siblings. This work suggests that adequate ADHD symptom control during the early morning period may be an unmet need for school-age children with ADHD being treated with stimulants. More work is needed to confirm this finding and determine the degree to which symptom control at other times of day is also an unmet need.

594 Low ambient temperature and midnight to early morning period onset highly predict testicular torsion among acute scrotums in Japanese male patients younger than 30

594 Low ambient temperature and midnight to early morning period onset highly predict testicular torsion among acute scrotums in Japanese male patients younger than 30

Development of a chlorine chemistry module for the Master Chemical Mechanism

Abstract. The chlorine atom (Cl·) has a high potential to perturb atmospheric photochemistry by oxidizing volatile organic compounds (VOCs), but the exact role it plays in the polluted troposphere remains unclear. The Master Chemical Mechanism (MCM) is a near-explicit mechanism that has been widely applied in the atmospheric chemistry research. While it addresses comprehensively the chemistry initiated by the OH, O3 and NO3 radicals, its representation of the Cl· chemistry is incomplete as it only considers the reactions for alkanes. In this paper, we develop a more comprehensive Cl· chemistry module that can be directly incorporated within the MCM framework. A suite of 205 chemical reactions describes the Cl·-initiated degradation of alkenes, aromatics, alkynes, aldehydes, ketones, alcohols, and some organic acids and nitrates, along with the inorganic chemistry involving Cl· and its precursors. To demonstrate the potential influence of the new chemistry module, it was incorporated into a MCM box model to evaluate the impacts of nitryl chloride (ClNO2), a product of nocturnal halogen activation by nitrogen oxides (NOX), on the following day's atmospheric photochemistry. With constraints of recent observations collected at a coastal site in Hong Kong, southern China, the modeling analyses suggest that the Cl· produced from ClNO2 photolysis may substantially enhance the atmospheric oxidative capacity, VOC oxidation and O3 formation, particularly in the early morning period. The results demonstrate the critical need for photochemical models to include more detailed chlorine chemistry in order to better understand the atmospheric photochemistry in polluted environments subject to intense emissions of NOX, VOCs and chlorine-containing constituents.