Early Life Stages Of Common Carp Research Articles

Effects of selected tricyclic antidepressants on early-life stages of common carp (Cyprinus carpio)

Despite the fact that pharmaceuticals occur in surface water in low concentrations, they can still have adverse effect on aquatic biota, because these substances are designed to have a specific mode of action even at low concentrations. To our knowledge, only little is known about the long-term effect of tricyclic antidepressant residues on non-target animals, especially fish. Hence, the aim of this study was to assess the effect of subchronic exposure to selected tricyclic antidepressants – namely, amitriptyline, nortriptyline, and clomipramine and their mixture – on early-life stages of common carp (Cyprinus carpio). Two embryo-larval toxicity tests with common carp were performed, each taking 30 days. For the experiment, three tricyclic antidepressants as well as their mixtures were tested at three different concentrations – 10, 100 and 500 μg/L. Hatching and mortality were recorded twice a day. In addition, samples were taken at regular intervals in order to record developmental stage, morphometric and condition characteristics and morphological anomalies. At the end of the test, additional samples were taken for histopathological examination and also for the determination of antioxidant and biotransformation enzyme activity and lipid peroxidation and protein carbonylation. Long-term exposure resulted in a significant increase in mortality, developmental retardation, morphological anomalies, and pathological changes in brain, heart, and cranial and caudal kidney. In addition, changes in antioxidant enzyme activity as well as increased lipid peroxidation were observed, even at the lowest tested concentrations. Hence, environmentally relevant concentrations of TCAs have the potential to cause harmful effects on early-life stages of fish.

NeemAzal T/S - toxicity to early-life stages of common carp (Cyprinus carpio L.)

In the European Union, the use of the insecticide NeemAzal T/S (standardised variant 1% of the active ingredient azadirachtin) is authorised in organic agriculture. The objective of this study was to determine the toxic effects of NeemAzal T/S at concentrations of 3, 10, 30, and 60 mg/lon the morphometric and condition characteristics, mortality, hatching, and histopathology of early-life stages of common carp (Cyprinus carpio L.) as a non-target aquatic organism, as well as related effects of NeemAzal T/S on selected indices of oxidative stress in the same organism. The embryo-larval toxicity test was performed according to OECD Guidelines 210 (Fish, Early-life Stage Toxicity Test). NeemAzal T/S exposure induced slow hatching on the first day and increased cumulative mortality in groups exposed to the insecticide. No effect on morphometric or condition characteristics was observed after 31 days of exposure. Histopathological changes of the gills were found at the highest concentration of 60 mg/lof NeemAzal T/S. Exposure at 30 mg/lwas associated with significantly (P < 0.01) increased glutathione peroxidase and glutathione S-transferase (P < 0.05) activities compared to the control group. The content of oxidised lipids was significantly higher (P < 0.05) in the 3, 10, and 30 mg/l experimental groups than in the controls. NeemAzal T/S exerted a significant negative influence on histopathological parameters in the embryo and larvae of common carp, as well as on the indices of oxidative stress in the same organism.

Effects of prometryne on early life stages of common carp (Cyprinus carpio L.)

Toxicity of prometryne to early life stages of common carp was assessed. On the basis of accumulated mortality in the experimental groups lowest observed-effect concentration (LOEC) was estimated as 1100 µg/l; and no observed-effect concentration (NOEC) was 850 µg/l. Fulton's condition factor was significantly lower than in controls in fish exposed to 4000 µg/l after 7, 14, and 21 days. By day 14, fish exposed to 4000 µg/l prometryne showed significantly lower mass and total length compared to controls. Fish exposed the 1200 and 4000 µg/l showed delay in development, severe hyperaemia in gill, liver, and caudal and cranial kidney. Subchronic prometryne exposure of early-life stages of common carp at concentrations of 1200 and 4000 µg/l affected their survival, growth rate, early ontogeny, and histology.