The aim of this study was to investigate the effect of maturation on vertical jumping performance, in adolescent boys and girls, concerning plantar flexor activity and Achilles tendon (AT) stiffness. Thirty-nine adolescents were tested in a counter-movement jump (CMJ) at three different time points: 18 and 9 months before peak height velocity (PHV) and at PHV. The EMG activity of the medialis gastrocnemius (MG) and tibialis anterior (TA) muscles was evaluated, in relation to jump height. Boys showed higher jumping ability and AT stiffness than girls. Additionally, boys revealed increased eccentric (ecc) and concentric (con) MG activity, along with decreased ecc and con TA activity, near PHV. On the other hand, girls showed increased ecc and con TA/MG co-contraction compared to boys, mainly near PHV. In conclusion, a different mechanism of vertical jumping performance is adopted between early adolescent boys and girls. Nevertheless, no notable alterations in jumping capability were detected over time, indicating that the maturation process does not influence stretch-shortening cycle (SSC) performance.