Dynamic Thiol-disulphide Homeostasis Research Articles

Dynamic thiol–disulphide homeostasis as a biomarker for predicting the development of contrast medium-associated acute kidney injury in the endovascular treatment of peripheral arterial disease: should intravenous N-acetylcysteine be given before the procedure?

To determine the predictive ability of serum thiol-disulphide levels for contrast medium-associated acute kidney injury (CA-AKI) after endovascular treatment (EVT) of peripheral arterial disease (PAD) and evaluate the efficacy of intravenous N-acetylcysteine (NAC) in preventing CA-AKI. This double-blind, randomised controlled study included 85 consecutive adult patients who underwent EVT for PAD. Patients were divided into NAC negative (NAC-) and positive (NAC+) groups. While the NAC- group received only 500 ml saline, the NAC+group received 500 ml saline plus intravenous 600 mg NAC before the procedure. Intra- and intergroup patient characteristics, procedural details, preoperative thiol-disulphide levels, and ischaemia-modified albumin (IMA) levels were recorded. There was a significant difference between NAC- and NAC+groups regarding native thiol, total thiol, disulphide/native thiol ratio (D/NT), and disulphide/total thiol ratio (D/TT). There was also a significant difference between the NAC- (33.3%) and NAC+ (13%) groups in CA-AKI development. Logistic regression analysis showed that the D/TT (OR 2.463) and D/NT (OR 2.121) were the most influential parameters for CA-AKI development. In the receiver operating characteristic (ROC) curve analysis, the sensitivity of native thiol to detect the development of CA-AKI was 89.1%. The negative predictive values of native thiol and total thiol were 95.6% and 94.1%, respectively. The serum thiol-disulphide level can be used as a biomarker to detect CA-AKI development and reveal patients with a low risk for CA-AKI development before EVT of PAD. Furthermore, thiol-disulphide levels allow for the indirect quantitative monitoring of NAC. Preprocedural intravenous NAC administration significantly inhibits CA-AKI development.

Dynamic thiol-disulphide homeostasis and ischemia modified albumin levels in neonatal calf diarrhea

The aim of this study was to determine dynamic thiol-disulphide homeostasis (TDH) parameters and ischemia modified albumin (IMA) levels in calves with neonatal diarrhea and compare with healthy controls. A total of 50 calves were included in the study. There were 25 calves in both diarrhea and healthy groups. Serum native thiol, total thiol, disulfide and IMA levels were measured using new methods. Native thiol (P=0.025) and total thiol (P=0.041) values decreased significantly in calves with neonatal diarrhea compared to the healthy control group. Disulphide (P= 0.133), disulphide/native thiol ratio (P=0.001) and IMA (P=0.0018) parameters were lower in healthy group, and the difference between the two groups was significant for the parameters other than disulphide. This study shows that TDH is impaired in neonatal calf diarrhea and IMA levels are increased due to oxidative stress.

Relationship of thiol/disulphide homeostasis with oxidative stress parameters in non-diabetic, prediabetic and type 2 diabetic Turkish women

Background and Aims: Deterioration of thiol/disulphide homeostasis (TDH) is found in several diseases, including diabetes. This study aimed to examine the association between TDH and oxidative stress parameters in nondiabetics, prediabetics and newly diagnosed type 2 diabetics. Methods: A total of 26 non-diabetic, 24 prediabetic and 19 type 2 diabetic women were involved in our study. They all applied to Zonguldak Bulent Ecevit University, Health Practice and Research Center, Endocrinology and Metabolism Diseases, Diabetes Polyclinic to be tested for type 2 diabetes mellitus. The demographic and laboratory data were collected from the patient files. Oxidative stress parameters and dynamic TDH were investigated using ELISA kits. Results: Total oxidant status (TOS), total thiol and disulphide levels were significantly higher in type 2 diabetics than the non-diabetics (24.24±14.93 versus 14.14±12.19, 646.47±75.51 versus 470.88±180.85, and 179.32±51.24 versus 91.85±40.29, respectively). In type 2 diabetics, a positive correlation between TOS and native thiol, total thiol and disulphide levels was found (P=0.000). In prediabetics, a significant positive correlation was found between total antioxidant capacity and total thiol levels (P<0.05), and also between arylesterase and native and total thiol levels (P<0.05). Conclusion: The elevation of oxidative stress and the deterioration of TDH might cause the formation of symptoms related to high blood glucose levels in type 2 diabetics.

Dynamic Thiol Disulphide Homeostasis in the Follow-Up of the Prognosis of Patients Treated for COVID-19 in the Intensive Care Unit.

Introduction: Evaluation of the prognosis in the early period of intensive care patients and arranging the treatment accordingly is of vital importance. In the present study, we investigated whether serum thiol/disulphide concentration can be used in the follow-up of prognosis in the early period in patients with COVID-19 under intensive care.Methods: The study included 25 patients [their ages were between 19 and 92; 10 (40%) were male and 15 (60%) were female] who were diagnosed with COVID-19 and treated in the intensive care unit (ICU). The patients were followed for four weeks. On the first, third, and fifth days of intensive care treatment, venous blood samples were taken from the patients to analyze the thiol/disulphide parameters, and coma scores were calculated. Statistical analyses were conducted to evaluate the relationship between thiol/disulphide levels and the prognosis of COVID-19 patients.Results: At the end of the four-week follow-up of the patients included in the study, 9 were discharged and 16 died. In patients who died, the relationship between thiol/disulphide homeostasis parameters and coma scores was not statistically significant. Meanwhile, in discharged patients, the relationship between disulphide concentration, total thiol, and coma scores was statistically significant.Conclusion: The relationship between thiol/disulphide homeostasis and coma scores in COVID-19 patients treated in the intensive care unit may help to evaluate the prognosis of the disease in the early period, thus the effectiveness of medical intervention.

Dynamic thiol disulphide homeostasis in patients with surfer's eye: a case-control study.

The role of oxidative stress in the pathogenesis of pterygium is still unclear. However, abnormal thiol disulfide homeostasis levels are involved in the pathogenesis of various systemic or ocular diseases. We aim to analyze dynamic thiol disulphide homeostasis in patients suffering from conjunctival pterygium using a contemporary technique. Thirty-eight subjects suffering from pterygium and 35 age-gender matched healthy volunteers were recruited for the study. For each case, total thiol, disulfide and native thiol levels in blood were obtained. Additionally, the ratio of disulfide over total thiol, native thiol over total thiol and disulfide over native thiol were computed. The level of median native thiol was lower in pterygium group (318.2µmol/L vs. 333.4µmol/L) and median disulfide was slightly higher in pterygium group (24.3µmol/L vs. 22.8µmol/L) compared to control group. Both disulfide over total thiol and disulfide over native thiol ratios were higher in pterygium group, ratio of native thiol over total thiol was found to be higher in control group. Nevertheless, none of those differences were statistically significant at 95% confidence level. Notably, correlation test pointed to a negative correlation both between pterygium grade and native thiol and between total thiol and pterygium grade in pterygium group (P = 0.03 and 0.02, respectively). A negative correlation hinting that slightly weakened dynamic thiol disulphide homeostasis in subjects with pterygium, a local ocular disease. Further studies with larger sample sizes may shed light on this potential relationship and justify systemic antioxidant therapies in these cases.

The association of dynamic thiol-disulphide balance with white-coat hypertension.

Dynamic thiol-disulphide homeostasis is an indicator of the antioxidant system that interacts early with the oxidative environment. This study aimed to assess the dynamic thiol/disulphide balance in individuals with white-coat hypertension (WCH). This cross-sectional study included a total of 117 individuals who presented to our outpatient cardiology clinic. Seventy patients were diagnosed with WCH. All blood samples were obtained from the patients after a minimum of 8 h of fasting, centrifuged immediately, stored in Eppendorf tubes, and protected at -80°C. The two groups were well-balanced in terms of age, gender, and BMI. Native thiol and total thiol levels were lower in the WCH group than in normotensive individuals. However, disulphide levels were higher in the former. Serum disulphide concentration positively correlated with 24-h SBP, and 24-h DBP. In backward stepwise logistic regression analysis, serum disulphide [odds ratio (OR) = 1.165 (1.089-1.245), 95% confidence interval (CI), P < 0.001] and serum total thiol levels [OR = 0.992 (0.984-1.000), 95% CI, P = 0.050] were independently associated with WCH. We demonstrated that dynamic thiol/disulphide balance shifted strongly towards disulphide formation due to thiol oxidation in patients with WCH.

Plasma thiol/disulphide homeostasis changes in patients with relapsing-remitting multiple sclerosis.

Multiple sclerosis (MS) is a neuroinflammatory disease and inflammation and oxidative stress play important roles in its pathology. Thiol/disulphide homeostasis (TDH) is a special oxidative stress biomarker that has been found to be affected in several disorders including MS. There is no study demonstrating the effects of attack status of the relapsing-remitting multiple sclerosis (RRMS) patients on TDH levels. Our aim was to determine TDH levels in three different periods of RRMS patients and healthy individuals. The study was carried out in 29 patients with RRMS without a prior attack in the last twelve months (MS Control), 21 RRMS patients having a clinical acute attack within the last week (MS relapse), 12 of 21 MS relapse patients one month after the onset of attack and following 1000mg methylprednisolone for 7days (MS Remission) and 30 age- and sex-matched healthy individuals. TDH status was determined using an automated spectrophotometric analysis method. TDHlevels in all patient groups and control subjects were compared with each other. The lowest native thiol, total thiol levels and native thiol/total thiol ratio were found in the MS relapse patients in comparison to the MS control, MS remission groups and healthy controls. In contrast, disulphide levels, disulphide/native thiol and disulphide/total thiol ratios were highest in the MS relapse group compared to the other patient groups and healthy subjects. Our findings indicate that increased oxidative stress in RRMS patients is reflected with decreased native and total thiol and increased disulphide levels. Since the formation of disulphide bonds is reversible, the progression of RRMS involving abnormal TDH may be controlled, converting disulphides to thiols. So, we suggest determining the dynamic TDH status as a novel and special biomarker in the diagnosis and prognosis of the RRMS patients.

Plasma thiol/disulphide homeostasis changes in patients with restless legs syndrome.

Restless legs syndrome (RLS) is a common neurological condition. Oxidative stress plays an important role in its pathogenesis. Thiol-disulphide homeostasis (TDH) is a new biomarker of oxidative stress. We studied plasma TDH to determine whether TDH could be used as a new biomarker for RLS and evaluated correlations between TDH and various disease severity rating scales. A total of 25 RLS patients and 25 healthy controls were included into the study. TDH status was determined using an automated spectrophotometric analysis method and correlations were analyzed between the TDH status and various disease rating scales in the RLS patients. Plasma total (401±27μmol/L) and native thiol (354±30μmol/L) levels were significantly lower, but disulphide level (24±6μmol/L) was significantly (<0.0001) higher in the RLS patients compared to the controls (455±36, 424±37, 15±5μmol/L, respectively). The disulphide/native thiol and disulphide/total thiol ratios increased, in contrast, native thiol/total thiol ratio decreased significantly in the RLS patients compared to thehealthy controls (<0.0001). The disulphide levels correlated positively with age and various rating scores of the RLS patients. International Restless Legs Syndrome Study Group (IRLSSG) rating score and age correlated negatively with the total and native thiol levels. Our findings indicate increased oxidative stress in the RLS patients reflected by decreased native and total thiol, and increased disulphide levels and positive correlations between the disulphide levels and various rating scores. We suggest dynamic TDH status to be used as a novel biomarker for the diagnosis and follow-up of the RLS patients.

Thiol-disulphide balance and total oxidant-antioxidant status in patients with chronic hepatitis C.

To assess the dynamic thiol/disulphide homeostasis (DTDH) and total oxidant/antioxidant status in patients with hepatitis C virus (HCV) infection and to evaluate their association with HCV-RNA levels. Levels of serum total thiol (TT), native thiol (NT), disulphide (DS), total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) as oxidative stress markers were determined in 162 individuals, including 74 patients with HCV infection and 88 non-HCV controls. HCV genotypes and HCV-RNA levels of the patients were recorded. The NT, TT and TAS levels and NT/TT ratio were significantly lower in the HCV group compared with the control group. On the contrary, DS, TOS and OSI levels and DS/NT and DS/TT ratios were significantly higher. Patients with high HCV RNA levels (> 650000IU/mL) had higher DS levels than patients with low HCV-RNA levels (<650000IU/mL). Genotype 1 was observed in 68.9% of patients with HCV. Levels of oxidative stress parameters were similar between genotype 1 and other genotypes (2, 3 and 5). No significant correlations were found between oxidative stress markers and albumin, alanine aminotransferase, aspartate aminotransferase, bilirubin and HCV-RNA levels in patients with HCV infection. A negative correlation was found only between OSI and albumin. Our results suggest that DTDH shifts towards the DS direction because of thiol oxidation in HCV-infected patients. Furthermore, DS levels were significantly higher in patients with high HCV-RNA levels compared with patients with low HCV-RNA levels.

Dynamic Thiol Disulphide Homeostasis In Patients Diagnosed With Acute Pulmonary Thromboembolism In Emergency Department

Dynamic Thiol Disulphide Homeostasis In Patients Diagnosed With Acute Pulmonary Thromboembolism In Emergency Department

A new biomarker of oxidative stress in obstructive jaundice: Dynamic thiol-disulphide homeostasis

A new biomarker of oxidative stress in obstructive jaundice: Dynamic thiol-disulphide homeostasis

A comprehensive study of oxidative stress in patients with chronic otitis media

Objective: The purpose of this research study was to evaluate dynamic thiol/disulphide homeostasis (TDH) as a novel oxidative stress marker in participants with noncholesteatomatous chronic otitis media (ncCOM) and cholesteatomatous chronic otitis media (cCOM), as well as to investigate lipid hydroperoxide (LOOH), total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) markers and compare the results with a healthy control group. Methods: This research was carried out at Harran University Medical Faculty Ear-Nose-Throat Department, between April 2017 and April 2019. This was a prospective controlled trial study including 121 participants, 40 ncCOM, 39 cCOM and 42 healthy controls. Total thiol, native thiol, disulphide levels and LOOH, TAS, TOS levels were measured in plasma of all patients and healthy volunteers. Results: In our study, we found that total thiol, native thiol levels, native thiol/total thiol ratios and TAS levels were significantly lower in cCOM patients compared with the control group (p<0.01). Disulphide/native thiol, disulphide/total thiol ratios, LOOH, TOS and OSI levels were significantly increased in the cCOM group compared with the control group (p<0.01). Conclusion: Oxidative stress (OS) is believed to be one of the underlying reasons for COM pathogenesis. In this research, we analyzed dynamic TDH as a novel OS marker using a novel improved fully automatic colorimetric technique, along with other parameters. This study may shed light on understanding the underlying reasons for the pathogenesis of COM and identify potential targets for antioxidant drug treatment.

MP10-02 A NOVEL METHOD PREDICTING OBSTRUCTIVE RENAL INJURY: THIOL-DISULPHIDE HOMEOSTASIS

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology II (MP10)1 Apr 2020MP10-02 A NOVEL METHOD PREDICTING OBSTRUCTIVE RENAL INJURY: THIOL-DISULPHIDE HOMEOSTASIS Emre Tokuc, Ahmet Urkmez*, Unal Can, Rahime Orak, Gulistan Gumrukcu, Ozcan Erel, Musab Ali Kutluhan, and Metin Ozturk Emre TokucEmre Tokuc More articles by this author , Ahmet Urkmez*Ahmet Urkmez* More articles by this author , Unal CanUnal Can More articles by this author , Rahime OrakRahime Orak More articles by this author , Gulistan GumrukcuGulistan Gumrukcu More articles by this author , Ozcan ErelOzcan Erel More articles by this author , Musab Ali KutluhanMusab Ali Kutluhan More articles by this author , and Metin OzturkMetin Ozturk More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000830.02AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Obstructive uropathies can lead to progressive nephron loss at all ages. Renin-angiotensin system and other pathways induced by the increase of intraglomerular pressure due to obstruction cause renal ischemia leading to hypoxia and oxidative stress and can result in renal fibrosis, necrosis and apoptosis if not eliminated. Various biomarkers have been investigated in obstructive pathologies to date. Thiols are organic compound formed by hydrogen, sulfur and carbon atoms. In cases of oxidative stress, transient disulphide bonds are established between thiol groups. Our aim is to show the importance of the dynamic thiol-disulphide homeostasis in predicting renal injury in ureter obstruction. METHODS: 24 Wistar Albino rats were randomized and divided into 3 groups. Groups were named as sham, partial and complete. 2 cc venous blood was taken from each rat for the measurement of creatinine and thiol disulphide values and renal scintigraphy was performed. Only an abdominal incision was done in the sham group. The left ureter of the partial group was dissected and gently buried into the psoas muscle. In the complete group, the left ureter was dissected and ligated from two separate places and cut in the middle. On the postoperative 7th day, 2 cc venous blood samples were taken for biochemical examinations, control renal scintigraphy was done, left nephrectomy was performed and the kidneys were analysed histopathologically. RESULTS: There was statistically significant difference between the groups in terms of postoperative native thiol and total thiol values (p: 0.000, p: 0.001). There was also a significant difference in preoperative and postoperative comparisons of these parameters in partial and complete obstruction groups (p: 0.012, p: 0.018). There was a significant difference in terms of postoperative disulphide/native thiol and native thiol/total thiol ratios in complete obstruction group compared to the other groups (p1: 0.011; p2: 0.040 and p1: 0.015; p2: 0.028). A statistically significant association was found between native thiol, total thiol, left kidney function loss percentage and creatinine change values and histopathological EGTI scores (r: -0,867, r: -0,815, r: 0,745, r: -0,911, p: 0.000). CONCLUSIONS: Thiol-disulphide homeostasis is a valuable hematological parameter in predicting renal injury in obstructive uropathy. Decrease in levels of native thiol and total thiol is associated with pathological changes in the kidneys. Disulphide/native thiol and native thiol/total thiol ratios may be indicators of complete obstruction. Source of Funding: None © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e125-e125 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Emre Tokuc More articles by this author Ahmet Urkmez* More articles by this author Unal Can More articles by this author Rahime Orak More articles by this author Gulistan Gumrukcu More articles by this author Ozcan Erel More articles by this author Musab Ali Kutluhan More articles by this author Metin Ozturk More articles by this author Expand All Advertisement PDF downloadLoading ...

The Impact of Acupuncture Treatment on dynamic thiol–disulphide homeostasis and ischemia-modified albumin levels to assess the oxidative stress in Migraine Patients

The aim of this study was to investigate the effect of acupuncture on dynamic thiol–disulphide homeostasis and ischemia-modified albumin (IMA) levels as a novel oxidative stress parameter in migraine patients. Acupuncture treatment was applied to the 22 volunteer migraine patients. The acupuncture treatment consists of 5 sessions with 2 sessions per week. Blood samples have been collected before performing acupuncture, after the 1st session and after the 5th session of the acupuncture. And for the control group blood samples were collected only once. A total of 44 participants were included in the study, and 24 of whom were diagnosed with migraine. The total thiol, native thiol and ischemia-modified albumin (IMA) levels in the serum were measured in all patients and healthy individuals. The dynamic disulphide bond and ischemia-modified albumin (IMA) levels were calculated from these values. The total and native thiol levels of migraine patients participating in the study were found to be significantly higher than the total and native thiol levels of healthy individuals. The total thiol, native thiol, and dynamic disulphide bond levels, and ischemia-modified albumin (IMA) were correlated with attack frequency, pain intensity, or migraine type. Thiol-disülfide homeostasis can play roles in the etiology and severity of Migraine.

Evaluation of dynamic thiol-disulphide homeostasis in obstructive uropathy.

To evaluate the ability of thiol-disulphide homeostasis to predict renal injury in ureteral obstruction by matching renal scintigraphy and pathological findings in an experimental rat model. 24 rats were randomized and divided into 3 groups as sham, partial and complete. Blood samples for biochemical evaluations and dimercaptosuccinic acid (DMSA) renal scintigraphy were performed at the beginning and on the 7th day postoperatively. Only a midline abdominal incision was done in sham group. Left ureter of partial group was dissected and gently buried into the psoas muscle. In complete group, left ureter was dissected and ligated. Statistically significant association was found between left kidney function loss percentage, native thiol, total thiol and creatinine alterations and pathological EGTI (Endothelial, Glomerular, Tubular, Interstitial) scores (r = - 0.867, r = - 0.815, r = 0.745, r = - 0.911; p = 0.000). Statistically significant difference was detected between groups in terms of postoperative native thiol and total thiol values (p = 0.000, p = 0.001). There was also a significant difference in preoperative and postoperative comparisons of these parameters in partial and complete obstruction groups (p = 0.012, p = 0.018). There was a significant difference in terms of postoperative disulphide/native thiol and native thiol/total thiol ratios in complete obstruction group compared to other groups (p1 = 0.011, p2 = 0.040; p1 = 0.015, p2 = 0.028). Association with scintigraphic and pathological results empowers the value of this parameter. Disulphide/native thiol and native thiol/total thiol ratios indicate complete obstruction. These findings may shed light to more comprehensive studies.

The association of dynamic thiol-disulfide homeostasis and inflammatory markers in patients with polycystic ovary syndrome

ObjectivePolycystic ovary syndrome (PCOS) is a common hormonal disorder among women of reproductive age characterized by irregular menstruation and hirsutism and is associated with an increased risk for cardiovascular diseases. Increased inflammatory response and oxidative stress may also present in these patients. In this study, we aimed to compare the neutrophil-to-lymphocyte ratio (NLR), mean platelet volume (MPV), and dynamic thiol-disulphide homeostasis (dTDH) between the patients with PCOS and healthy individuals and to investigate the correlation between these parameters and cardiovascular risk factors in patients with PCOS. Materials and methodsA total of 60 participants were included in this study. The patient group consisted of 36 patients who were diagnosed with PCOS and the control group consisted of 24 healthy individuals without PCOS. Complete blood count and hormonal tests were performed using blood samples. The NLR, MPV, and dTDH were compared between the patient and control groups. ResultsThere was no statistically significant difference in the native thiol, total thiol, disulphide levels and disulfide/native, disulfide/total and native/total thiol ratios between the patient and control groups (p = 0.494, p = 0.446, p = 0.338, p = 0.717, p = 0.723, and p = 0.717, respectively). In addition, there was no statistically significant difference in NLR and MPV between the groups (p = 0.531 and p = 0.196). ConclusionOur study results showed no significant difference in the NLR, MPV, dTDH levels, and inflammatory biomarkers including leukocyte count between the PCOS patients and healthy controls. Based on these findings, we conclude that the diagnosis of PCOS alone in overweight patients has no considerable effect on these biomarkers.

Evaluation of thiol-disulphide homeostasis in patients with chronic hepatitis B

Aim: To evaluate the relationship between dynamic thiol-disulfide homeostasis which is a novel oxidative stress marker and levels of HBV DNA, and the oxidant-antioxidant balance. Material and Method: In the controlled study which included chronic hepatitis B (CHB) patients and healthy volunteers, dynamic Thiol-disulphide homeostasis (TDH) was measured using a novel automated method developed by Erel. Disulfide / total thiol (%), disulfide / native thiol (%), and native thiol / total thiol (%) rates were calculated using the previously determined concentrations of disulfides, native thiols, and total thiols. Results: Of thiol / disulfide homeostasis parameters, native thiol, total thiol, and disulfide levels were statistically lower in the CHB patient group (p <0.05). As a result of the correlation analyses, a significant negative correlation was determined between HBV DNA levels and disulfide / native thiol, disulfide / total thiol, and native thiol / total thiol parameters (p <0.05). Conclusions: Our results suggest that oxidative stress increases with the rise in HBV-DNA levels and that the antioxidant defense may have weakened.

Dynamic thiol-disulphide homeostasis in grade 3-4 gonarthrosis

Objective: We aimed to determine thiol-disulphide homeostasis, which plays a vital role and to investigate the relationship among homeostatic parameters and disease. Methods: In this prospective study, we enrolled 38 patients with osteoarthritis (31 females and 7 males) and 38 healthy controls (30 females, 8 males volunteers). Diagnosis of osteoarthritis was made according to the American College of Rheumatology Criteria. The severity of osteoarthritis was assessed and classified according to the Kellgren-Lawrence grading scale. Results: The mean age was 63.8 (range; 53-74) years in the osteoarthritis group and 65.6 (range; 55-75) years in the control group. There were no significant differences between the patients and controls in respect to age, gender and body mass index (p &amp;gt; 0.05). Serum albumin (p = 0.605) and total protein levels (p = 0.605) between patients and controls were similar. In the osteoarthritis group disulphide/ native thiol percent ratios and disulphide/ total thiol percent ratios were found to be statistically higher (p = 0.002 and p = 0.002; respectively) and native/ total thiol percent ratios were significantly lower than that of the control group (p = 0.002). Conclusions: Thiol-disulphide homeostasis is weakened in osteoarthritis, and the balance shifts to the disulphide bond formation side. Substitution of thiol deficiency and correction of thioldisulphide imbalance may be beneficial in the managing treatment of the disease. Further studies may be needed for evaluating articular fluid thiol-disulphide homeostasis.

Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancer

Abstract Aim Data about the relationship between oxidative stress and cancer pathogenesis has been increased in recent years. Thiol and disulphide play an important role in cell signal mechanisms, antioxidant protection, and detoxification. In this study, we aimed to evaluate the role of Thiol-Disulphide homeostasis (TDH) in colorectal cancer (CRC) by using a new method. Material and Method The patients (pts) who diagnosed with CRC and healthy control subjects were included to study. Serum samples for the thiol-disulphide test were obtained at the time of diagnosis. TDH tests were measured by the automated spectrophotometric method by describing Erel and Neselioglu. Thiol-disulphide homeostasis was also evaluated according to tumor stage and localization. Results Eighty-eight pts with CRC and 110 control were enrolled. Native thiol (NT), disulphide and total thiol (TT) levels were significantly lower in patients compared with the control arm (Median NT: 402–424, p = 0.003; median Disulphide 18.7–21, p = 0.011; median TT: 437–467, p = 0.001). Thiol/disulphide balance was also maintained (p = 0.149). TT and NT levels were not differed according to tumor localization whereas disulphide level was significantly higher in left-sided tumors than right-sided (19.9–13.07 respectively, p = 0.007). In addition, disulphide/NT ratio was also significantly higher in left-sided than right-sided (0.1–0.12 respectively, p = 0.015). Thus, the balance of dynamic TDH is disrupted in favor of disulphide between left-sided and right-sided tumor. There was also no significant difference between thiol-disulphide levels and tumor stage whereas thiol level tends to lower in stage 4 disease (p = 0.7). Conclusion This is the first trial that evaluates the relationship with dynamic TDH and CRC according to tumor stage and localization. Thiol and disulphide may play an important role in the pathogenesis of CRC.

Thiol-disulphide balance in infertility secondary to varicocele.

Oxidative stress plays an important role in the development of infertility secondary to varicocele. We aimed to investigate the dynamic thiol-disulphide homeostasis as an oxidative stress marker in the spermatic vein of infertility secondary to varicocele. Sixty-one patients with varicocele were included in the study. Blood was drawn from the median cubital vein and the testicular venous return side before the spermatic vein was separated during surgery. Total thiol, native thiol, disulphide, ischaemia modified albumin (IMA) and albumin values were measured from both the dilated spermatic vein and the median cubital vein. The disulphide/native thiol, disulphide/total thiol and native thiol/total thiol ratios were determined. The mean age of the patients was 27.0±6.68 (15-50) years. While the albumin, native thiol and total thiol values and the native thiol/total thiol ratio were significantly lower (p=0.004, p<0.001, p<0.001, p<0.001 respectively), the IMA value and the disulphide/native thiol and disulphide/total thiol ratios were significantly higher (p<0.001, p<0.001, p<0.001 respectively) in the samples taken from spermatic venous blood. Thiol-disulphide balance had deteriorated towards disulphide formation in the spermatic vein compared with the peripheral vein. Abnormal thiol-disulphide balance may be an independent risk factor for infertility secondary to varicocele.