Online signature verification is the process of using a dynamic signature verification system to confirm the writer’s identity. It can be used as a security system to confirm entrance applications and password substitutes, as well as a forensic tool to assist an expert’s investigation. This study proposes a novel online signature verification system based on a single-template strategy to improve performance in real-world scenarios. It employs discriminative mean signature template sets as well as fusion strategies of multiple local weighting and warping schemes, for dynamic time warping (DTW). The first step is to generate a set of user-specific mean signature templates for each feature using a recent time-series averaging method, namely, Euclidean barycenter-based DTW barycenter averaging. Then, using multiple and direct matching points between the mean signature templates and references for dependent and independent DTW, we obtain a local weighting estimate considering local stability sequences. Furthermore, we develop fusion strategies for calculating locally weighted DTW sets and concatenating them as a feature vector for each warping, followed by the construction of a support vector machine (SVM) classifier. Finally, in the verification phase, we use the single-template technique to compute a discriminative fused score using SVMs between the mean signature template sets and a query sample. The effectiveness of the proposed method is demonstrated by extensive experimental results obtained using three public online signature datasets: SVC2004 Task1/Task2 and MCYT-100.