In the long-term service, the wharf structure can be damaged by ship impact, wave load, and even earthquake, which will affect the safe production and smooth operation of the port. Based on the theory of structural dynamic response analysis and wavelet packet analysis principle, this paper established the damage identification index of the wharf frame structure. Combining with the finite element method and the dynamic response theory of the wharf frame structure, it set up a finite element analysis model of the dynamic response of the wharf frame structure under the action of multiple loads, with the impact load of the ship as the dynamic load under the non-damaged state and the different damaged states. In addition, the characteristic response point location was drawn up. Furthermore, the transient dynamic analysis and damage index analysis of the frame structure in the non-damaged and damaged state were conducted respectively. In addition, the model test and numerical simulation results were combined to compare and analyze the identification of damage indicators, so as to verify the identification effect of the established damage identification indicators on the structural damage, which lays a foundation for the next step of structural damage identification.