Dw For Zn Research Articles

Metal phytostabilization by mastic shrub (Pistacia lentiscus L.) and its root-associated bacteria in different habitats of Sardinian abandoned mining areas (Italy).

Pistacia lentiscus L. is an excluder metallophyte proposed for the revegetation and phytostabilization of metal-contaminated sites in the Mediterranean area. The present study aims at evaluating the linking between bacterial communities and plants spontaneously growing in ecosystems chronically impacted by mining activities. Environmental properties and metal accumulation into hypogeal and epigeal tissues were analyzed in wild plants of two contrasting habitats with extreme metal contamination (> 2300mg/kg for Zn, > 1100mg/kg for Pb, > 10mg/kg for Cd). The community structures of rhizospheric and root endophytic bacteria were fingerprinted by terminal restriction fragment length polymorphism of the 16S rRNA gene. The wild shrubs efficiently restrict the accumulation of the three major contaminants to the epigeal tissues in the two habitats under study (249 ± 68mg/kg dw for Zn, 43 ± 21mg/kg dw for Pb, and 1.4 ± 0.5mg/kg dw for Cd). Evidence was provided that the combined but not individual effect of environmental conditions (moisture, inorganic carbon, pH) and proportion between Zn and Cd in the mine substrate play a role in structuring rhizosphere bacterial communities. The observed changes in community structures of root endophytes were found to be strongly associated with Pb level in roots and substrate properties (inorganic carbon and Zn/Cd ratio). Overall, our study highlights the importance of the analysis of multifactorial interactions among mine substrate, plant, and microbes for understanding how the environmental context affects phytoremediation under real conditions.

Assessment of the transfer of heavy metals in seawater, sediment, biota samples and determination the baseline tissue concentrations of metals in marine organisms.

The majority of tissue-specific environmental quality standards (EQSs) considering metal tolerance are prior to the chemical-specific EQSs in aquatic organisms. However, metal baseline levels in marine organisms were very scarce. We explored the correlation between Hg, Cd, Pb, Cu, and Zn concentrations in water or sediments and those metal concentrations in marine organisms (crustacean, mollusc, and fish) by generalized additive models (GAMs) and executed a meta-analysis of Hg, Cd, Pb, Cu, and Zn contents in those three organisms by implementing cumulative frequency distribution analysis of individual metal distribution in a heavy metal-contaminated semi-enclosed Bay, China. Results showed that the average contents of Hg, Cd, Pb, Cu, and Zn were 0.042±0.01, 0.38±0.22, 1.72±0.65, 3.61±1.01, and 16.08±6.33 μg/L in water; 0.064±0.02, 0.42±0.04, 20.54±7.76, 28.97±3.90, and 96.74±35.11 μg/g dw in sediment; and 0.0049±0.0028, 0.52±0.28, 0.24±0.15, 11.05±6.95, and 21.12±4.47 μg/g dw in crustacean, 0.015±0.0087, 0.24±0.17, 0.08±0.02, 0.37±0.35, and 10.62±6.79 μg/g dw in mollusc; and 0.0038±0.0028, 0.065±0.05, 0.32±0.19, 2.01±0.59, and 16.04±4.97 μg/g dw in fish. The mercury content in mollusc presented a negative correlation with mercury content in sediment, while the content of other metals (Cd, Pb, Cu, and Zn) in organisms showed positive correlations with the content of those metals in water or sediment. We further obtained tissue-baseline-C5% in crustacean, mollusc, and fish which were 1.191, 3.341, and 0.014 μg/g dw for Cu; 0.013, 0.072, and 0.033 μg/g dw for Cd, 0.015, 0.027, and 0.052 μg/g dw for Pb; 9.515, 14.422, and 0.056 μg/g dw for Zn; and 0.0009, 0.004, and 0.0035 μg/g dw for Hg, respectively. However, there were no obvious relationships of the 4d-NOEC in laboratory toxicity tests with C5%, as well as C50% and 4d-LC50 or tolerance index a for Cu, Cd, Pb, Zn, and Hg in organisms. Our results pointed out the controversy of laboratory sensitive species toxicity results for deriving chemical-specific EQSs with field studies. We advocated to set up the metal concentration baselines in aquatic organisms and further served the tissue-specific EQSs, which are essential basis for geochemical recordings, bio-monitoring, and semi-enclosed bay management in the world.

Removal of multi-contaminants from water by association of poplar and Brassica plants in a short-term growth chamber experiment.

The plant association of Populus alba L. 'Villafranca', Brassica oleracea var. acephala sebellica (kale), and B. oleracea var. capitata 'sonsma' (cabbage) was exposed to Zn, Cd, and exogenous caffeine (13CFN)-contaminated water under growth chamber conditions. In the short term of treatment (15days), poplar increased the root dry biomass (+ 25%) and decreased the chlorophyll content in new leaves (- 32%), compared to control. On the contrary, cabbage decreased the root dry biomass, enhancing the shoot dry biomass (+ 50%). Heavy metals were mainly concentrated in plant roots and in poplar reached the highest concentrations of 705 ± 232.6 and 338 ± 85.5μgg-1 DW for Zn and Cd, respectively. The ability of poplar to accumulate more Zn and Cd than kale and cabbage in plant biomass was confirmed by heavy metal contents, following the order: poplar > kale = cabbage. However, poplar and Brassica sp. association was very useful for Zn and Cd decontaminations as reported by the bioconcentration factors (> 1). The concentration of 13CFN was below 2.4ngg-1 FW in poplar and 7.4ngg-1 FW in Brassica species, suggesting the caffeine uptake and degradation by plant association. Under our experimental conditions, the removal efficiency of the system was upper to 79%, indicating the capability of Populus-Brassica association to efficiently remove Zn, Cd, and 13CFN from mixed inorganic-organic-contaminated water in short term.

Salinity influences biosorption of heavy metals by the roots of the halophyte plant species Kosteletzkya pentacarpos

Dry powder of the roots from the wetland halophyte species Kosteletzkya pentacarpos grown in the presence or in the absence of 50mM NaCl was used for biosorption of Cd and Zn. Experiments were performed in batch conditions using 100mg of root powder and 10mL of contaminated solution. Optimal temperature was found to be 25°C and contact time of 15min was sufficient for maximal adsorption. Sorption efficiency was higher for Cd (88.8%) than for Zn (56.9%). The maximal adsorption value qm was 10.6μmolesg−1 DW for Cd and 7.98μmolesg−1 DW for Zn. The use of roots collected from plants grown for 16 weeks in the presence of NaCl improved sorption process, especially for Zn. This positive impact could be related to the salt-induced increase in mucilage pectic compounds and hemicellulose involved in heavy metal binding. In contrast, the presence of NaCl during the biosorption incubation reduced sorption efficiency as a result of a competition between Na+ and divalent cations for binding sites. Removal of the majority of pollutant was obtained by 0.2M (Cd) or 0.3M (Zn) HCl. It is concluded that roots of K. pentacarpos provide a valuable biological material for heavy metal retention.

Heavy metal content in vegetables and fruits cultivated in Baia Mare mining area (Romania) and health risk assessment.

Information about heavy metal concentrations in food products and their dietary intake are essential for assessing the health risk of local inhabitants. The main purposes of the present study were (1) to investigate the concentrations of Zn, Cu, Pb, and Cd in several vegetables and fruits cultivated in Baia Mare mining area (Romania); (2) to assess the human health risk associated with the ingestion of contaminated vegetables and fruits by calculating the daily intake rate (DIR) and the target hazard quotient (THQ); and (3) to establish some recommendations on human diet in order to assure an improvement in food safety. The concentration order of heavy metals in the analyzed vegetable and fruit samples was Zn > Cu > Pb > Cd. The results showed the heavy metals are more likely to accumulate in vegetables (10.8-630.6 mg/kg dw for Zn, 1.4-196.6 mg/kg dw for Cu, 0.2-155.7 mg/kg dw for Pb, and 0.03-6.61 mg/kg dw for Cd) than in fruits (4.9-55.9 mg/kg dw for Zn, 1.9-24.7 mg/kg dw for Cu, 0.04-8.82 mg/kg dw for Pb, and 0.01-0.81 mg/kg dw for Cd). Parsley, kohlrabi, and lettuce proved to be high heavy metal accumulators. By calculating DIR and THQ, the data indicated that consumption of parsley, kohlrabi, and lettuce from the area on a regular basis may pose high potential health risks to local inhabitants, especially in the area located close to non-ferrous metallurgical plants (Romplumb SA and Cuprom SA) and close to Tăuții de Sus tailings ponds. The DIR for Zn (85.3-231.6 μg/day kg body weight) and Cu (25.0-44.6 μg/day kg body weight) were higher in rural areas, while for Pb (0.6-3.1 μg/day kg body weight) and Cd (0.22-0.82 μg/day kg body weight), the DIR were higher in urban areas, close to the non-ferrous metallurgical plants SC Romplumb SA and SC Cuprom SA. The THQ for Zn, Cu, Pb, and Cd was higher than 5 for <1, <1, 12, and 6% of samples which indicates that those consumers may experience major health risks.

Selection of a halophytic plant for assessing the phytotoxicity of dredged seaport sediment stored on land

The filling of dry quarries in coastal areas with sediments dredged in seaports represents a potentially interesting method of recycling of these materials. However, this recycling requires the prior carrying out of an Environmental Risk Assessment of the scenario concerned. For this, the question arose as to the type of plants capable of developing on the surface of such a deposit and the method to implement for assessing the possible phytotoxicity of dredged sediments. To answer this question, we chose to work with halophytic plants to be free from the salt-related effect and to assess only the effect related to the toxic compounds present. Based on the objectives set, these works led to the use of common plants of the French coast, with direct seeding, and with pollution-sensitive plants. Three species of angiosperms, Armeria maritima, Anthemis maritima and Plantago coronopus, were finally tested. As a result of this work, Armeria maritima was retained as the most suitable plant for testing the possible phytotoxic effect of dredged marine sediments stored on land. The results obtained with this plant are as follows: germination of 40% of the seeds in 31days, produced biomass of 493mg FW in 6months and a capacity to bioaccumulate metal pollutants in roots with 350 and 720mg/kg DW for Zn and Cu, respectively.

Concentrations of Heavy Metals (Cu, Cd, Zn and Ni) and PAHs in Perna viridis Collected from Seaport and Non-seaport Waters in the Straits of Johore

In this study, the ranges of pollutants found in the soft tissues of Perna viridis collected from Kg. Masai and Kg. Sg. Melayu, both located in the Straits of Johore, were 0.85-1.58 μg/g dry weight (dw) for Cd, 5.52-12.2 μg/g dw for Cu, 5.66-8.93 μg/g dw for Ni and 63.4-72.3 μg/g dw for Zn, and 36.4-244 ng/g dry weight for ∑PAHs. Significantly (p < 0.05) higher concentrations of Cd, Cu, Ni, Zn and ∑PAHs in the mussels were found in the water of a seaport site at Kg. Masai than a non-seaport site at Kg. Sg. Melayu population. The ratios of low molecular weight/high molecular weight hydrocarbons (2.94-3.42) and fluoranthene/pyrene (0.43-0.45) in mussels from both sites indicated the origin of the PAHs to be mainly petrogenic. This study has demonstrated the utility of using the soft tissues of P. viridis as a biomonitor of PAH contamination and bioavailability in the coastal waters of Peninsular Malaysia.

Heavy Metals Accumulation in Some Vegetables and Soils in Istanbul

This work was designed to assess the concentration of six toxic metals in the soil and in unwashed and washed samples of different vegetable species (Petroselinum crispum, Brassica oleraceae var. acephala, Beta vulgaris var. cicla collected from six different sites (stream side, inner city, industrial, suburban, roadside, and rural) in Istanbul in 2007. Concentrations of Cd, Cr, Cu, Ni, Pb, and Zn were determined by using ICP-OES. It was found that the average highest values of metal accumulations were detected in unwashed samples. The correlation was significant between metal accumulations and the sites, where the vegetables were grown, in the industrial areas and along the roadsides. The highest and lowest average values in the vegetables varied between 0.24-0.89 μg/g dw for Cd, 3.73-14.04 μg/g dw for Cr, 1.47-5.19 μg/g dw for Cu, 3.13-13.65 μg/g dw for Ni, 29.28-87.00 μg/g dw for Pb, and 3.48-5.74 μg/g dw for Zn. The pattern of total metal contrations in the vegetables followed Pb>Cr>Ni>Zn>Cu>Cd.

Determination of mineral contents of wild-grown edible mushrooms

The aim of this study was to determine the mineral contents of wild edible mushrooms. The phosphorus (P), iron (Fe), calcium (Ca), zinc (Zn), magnesium (Mg), potassium (K), sodium (Na), copper (Cu) and manganese (Mn) contents of thirty wild-grown common edible mushrooms, collected from Erzurum province, Turkey, were analyzed. The minimum and maximum macronutrient contents of mushrooms were determined as mg/g dw for Mg (0.90–4.54), Ca (0.17–8.80), K (12.6–29.1), Na (0.03–4.85) and P (0.64–4.49), while minimum and maximum micronutrient contents of mushrooms were determined as mg/kg dw for Zn (26.7–185), Fe (50.1–842), Cu (9.23–107) and Mn (5.54–135). The potassium content was found to be higher than those of the other minerals in all the mushrooms. The K, P and Cu concentrations were determined to be highest in Suillus granulatus.

Heavy-metal distribution and risk assessment of sediment and fish from El-Mex Bay, Alexandria, Egypt

Heavy metals are increasingly being released into natural waters from geological and anthropogenic sources. The distribution of several heavy metals (Cr, Cu, Cd, Pb, Zn, and Hg) was investigated in muscle, gill, and liver in two different fish species seasonally collected in El-Mex Bay (autumn 2004–summer 2005). In order to evaluate the pollution status of the Bay, the concentrations of the selected metals in the labile and total fractions were analysed in sediment samples collected from eight sites in El-Mex Bay during autumn 2004. Also, the Index of Geoaccumulation (I geo) for the sediment was estimated. The total and labile fractions of the selected metals in sediment samples were 15.2 and 62.8 μg g−1 dw for Cu, 1.8 and 5.0 μg g−1 dw for Cd, 79.1 and 130.3 μg g−1 dw for Zn, 0.2 and 1.2 μg g−1 dw for Hg, 35.8 and 93.0 μg g−1 dw for Pb, and 13.9 and 31.0 μg g−1 dw for Cr. The concentrations of all metals were lower in flesh than those recorded in liver and gill due to their physiological roles. The metal pollution index for fish was calculated. Health hazard calculations for the contaminated sediments and fish consumption were calculated to evaluate the effect of pollution on health.

Triticum dicoccoides: An important genetic resource for increasing zinc and iron concentration in modern cultivated wheat

One major strategy to increase the level of zinc (Zn) and iron (Fe) in cereal crops, is to exploit the natural genetic variation in seed concentration of these micronutrients. Genotypic variation for Zn and Fe concentration in seeds among cultivated wheat cultivars is relatively narrow and limits the options to breed wheat genotypes with high concentration and bioavailability of Zn and Fe in seed. Alternatively, wild wheat might be an important genetic resource for enhancing micronutrient concentrations in seeds of cultivated wheat. Wild wheat is widespread in diverse environments in Tarkey and other parts of the Fertile Crescent (e.g., Iran, Iraq, Lebanon, Syria, Israel, and Jordan). A large number of accessions of wild wheat and of its wild relatives were collected from the Fertile Crescent and screened for Fe and Zn concentrations as well as other mineral nutrients. Among wild wheat, the collections of wild emmer wheat, Triticum turgidum ssp. dicoccoides (825 accessions) showed impressive variation and the highest concentrations of micronutrients, significantly exceeding those of cultivated wheat. The concentrations of Zn and Fe among the dicoccoides accessions varied from 14 to 190 mg kg−1 DW for Zn and from 15 to 109 mg kg−1 DW for Fe. Also for total amount of Zn and Fe per seed, dicoccoides accessions contained very high amount of Zn (up to 7 μg per seed) and Fe (up to 3.7 μg per seed). Such high genotypic variation could not be found for phosphorus, magnesium, and sulfur. In the case of modern cultivated wheat, seed concentrations of Zn and Fe were lower and less variable when compared to wild wheat accessions. There was a highly significant positive correlation between seed concentrations of Fe and Zn. Screening different series of dicoccoides substitution lines revealed that the chromosome 6A, 611, and 5B of dicoccoides resulted in greater increase in Zn and Fe concentration when compared to their recipient parent and to other chromosome substitution lines. The results indicate that Triticum turgidum L. var. dicoccoides (wild emmer) is an important genetic resource for increasing concentration and content of Zn and Fe in modern cultivated wheat.

시판 원예용 상토의 중금속 함량

Heavy metal contents in commercial horticultural growing media were investigated Fifty-three samples were collected nationwide during 2000 to 2001 and heavy metals in the samples were analyzed by ICP-OES after acid digestion and 0.1N HCl extraction. Average contents of total Cd, Cr, Co, Ni, Pb, and Zn by acid digestion in the growing media were 0.69, 35.7, 14.9, 15.5, 12.7, and 54.8 mg/kg DW, respectively. The ranges of heavy metal in the growing media were , , , , , DW for Cd, Cr, Co, Ni, Pb, and Zn, respectively. Heavy metal contents in the commercial horticultural growing media were showed lower levels than the permitted levels for quality control described by Korean Standard Analytical Method for Growing Media. Average contents and ranges of 0.1 N HCl extractable heavy metals were 0.08 and for Cd, 0.38 and for Cr, 1.45 and for Cu, 0.85 and for Ni, 0.84 and for Pb, and 30.68 and DW for Zn, respectively. These concentrations by 0.1 N HCl extraction showed much less levels than those of total heavy metals by acid digestion.

Cadmium and zinc in Mytilus edulis L. from the Bothnian sea and the northern baltic proper

Contents of Cd and Zn in the blue mussel, Mytilus edulis, collected on the coast of the southern Bothnian Sea and the northern Baltic proper, were determined using flame AAS. The observed concentrations ranged from 4·7 to 10·8 mg kg −1 dw for Cd and from 121 to 215 mg kg −1 dw for Zn. The Zn/Cd ratio was around 20 at a majority of the investigated locations. Cd exhibited an approximate two-fold gradual increase from south to north in the study area. It is likely that this increase was due largely to enhanced bioavailability for Cd at lower salinity. However, it is not possible to eliminate the influence from a generally elevated overall concentration of cadmium in the southern Bothnian Sea caused by natural and anthropogenic input. Compared to mussels from marine waters on the west coast of Sweden, Cd concentrations were 5–10 times higher, similarity indicating an influence of low salinity. Zn did not show these salinity correlated differences. The high Zn content found south of the outlet of the river Dalälven and gradually decreasing southwards was possibly caused by the high riverine input.