Duration Of Force Application Research Articles

Using an ER-specific optogenetic mechanostimulator to understand the mechanosensitivity of the endoplasmic reticulum

The ability of cells to perceive and respond to mechanical cues is essential for numerous biological activities. Emerging evidence indicates important contributions of organelles to cellular mechanosensitivity and mechanotransduction. However, whether and how the endoplasmic reticulum (ER) senses and reacts to mechanical forces remains elusive. To fill the knowledge gap, after developing a light-inducible ER-specific mechanostimulator (LIMER), we identify that mechanostimulation of ER elicits a transient, rapid efflux of Ca2+ from ER in monkey kidney COS-7 cells, which is dependent on the cation channels transient receptor potential cation channel, subfamily V, member 1 (TRPV1) and polycystin-2 (PKD2) in an additive manner. This ER Ca2+ release can be repeatedly stimulated and tuned by varying the intensity and duration of force application. Moreover, ER-specific mechanostimulation inhibits ER-to-Golgi trafficking. Sustained mechanostimuli increase the levels of binding-immunoglobulin protein (BiP) expression and phosphorylated eIF2α, two markers for ER stress. Our results provide direct evidence for ER mechanosensitivity and tight mechanoregulation of ER functions, placing ER as an important player on the intricate map of cellular mechanotransduction.

Success rates of single-thread and double-thread orthodontic miniscrews in the maxillary arch

AimThere is limited research on the clinical performance of double-thread orthodontic miniscrews. This study aimed to compare the stability of double-thread and single-thread orthodontic miniscrews and identify the potential associations between patient-related and location-related factors with miniscrew stability.MethodsThis retrospective cohort study involved 90 orthodontic miniscrews (45 single-thread, 45 double-thread) with identical dimensions (8 mm length, 1.6 mm diameter). The screws were inserted in various locations within the upper jaw of 83 patients (54 females, 29 males; mean age = 15.1 ± 2.4 years). Failure was defined as excessive mobility or loss of miniscrew after placement. The data recorded were patient age, gender, insertion site, side of insertion (buccal or lingual), duration of force application, and failure occurrence.ResultsThe overall success rate within the sample was 92.2%. Double-thread miniscrews exhibited a significantly higher success rate than single-thread miniscrews (P = 0.049), with 97.8% and 86.7% success rates, respectively. Gender, age, insertion location, and side of insertion did not show significant associations with failure (P > 0.05). Log-rank analysis revealed a significant difference between the two groups (P = 0.046), indicating a higher probability of survival for the double-thread design.ConclusionsThe overall success rate of orthodontic miniscrews was high in the present sample. Double-thread miniscrews placed in various locations within the maxillary arch demonstrated superior stability and survival rates compared to their single-thread counterparts. Therefore, double-thread miniscrews may be preferred when bone quality is inadequate, such as in young patients.

Improving headgear wear: why force level and direction of traction matter.

Empiric data on headgear wear are scarce. The aim was to examine a possible discrepancy between the duration of wearing and force application, and whether such a difference is influenced by force level or direction of traction. In this retrospective analysis, 122 consecutive patients were included. All were treated with headgear (three subgroups: high-pull headgear [n = 60], cervical-pull headgear [n = 32], and high-pull headgear in combination with an activator [n = 30]) and were monitored for three successive months using an electronic module. The device recorded chronographically the measured force magnitude and temperature, allowing to differentiate between the duration of headgear wear (recorded body temperature) and actual force application (recorded force). For all subgroups, the average recorded force application was lower than wear time (mean inactivity during wear: 15.9 ± 22.8 minutes/night). The direction of traction significantly influenced the extent and length of wear time without force application (P < 0.001): patients with cervical-pull headgear were more prone to inactive wear time (27.7 minutes/night) than patients with high-pull headgear (13.7 minutes/night) or with headgear-activator (7.8 minutes/night). The observed inter-individual variability of inactive wear time was considerable (0-134 minutes/night). The mean applied force was highly significantly associated with inactive wear time (correlation coefficient: -0.575; P < 0.001), and force levels below 250 g seem particularly related to episodes of inactivity. There is a clear incongruity between the duration of headgear wear and the duration of force application. Inactive wear time is influenced by the direction of traction and force level applied. Clinicians should be aware of the likelihood of periods of inactive wear time and researchers should search for options to reduce or even eliminate these periods.

Tailoring the Pressure Profile of TEA-CO2 Laser-Induced Shock Waves for Mechanical Forming and Separation Processes

For laser shock processes, the acting pressure can be increased by confining the propagation of the laser-induced plasma. Water or glass is commonly used as confinement for laser shock waves induced by Nd:YAG-lasers. This is not possible for the longer wavelength of TEA-CO2 lasers due to higher absorption coefficients. Instead, the acting pressure can be manipulated with a pressure cell above the workpiece. Two different pressure cells were designed to test the versatility of this concept. A cylindrical pressure cell design was used to confine the propagation of the laser-induced plasma and a parabolic pressure cell design was used to reflect the shock wave back to the workpiece. The effect of the pressure cells was analysed by time-resolved force measurements and evaluated for maximum force and force duration. This study shows that the cylindrical pressure cell strongly increases the maximum force while the parabolic pressure cell maximises the duration of force application. Furthermore, the pressure cells have been applied to the laser shock-based processes laser shock punching and LiSE hardness measurement. The cylindrical pressure cell strongly enhances the results of both processes, while the parabolic pressure cell shows only a minor effect.

Differences in Root Resorption between Root Canal treated and contralateral vital tooth during Orthodontic Tooth Movement: A Systematic Review

It is believed that root canal treated tooth apex goes under more resorption than contralateral vital tooth during orthodontic movement. The concept is still contradictory and therefore the aim of this systematic review was to search the evidence regarding this issue. The clinical trials performed on human subjects with vital and contralateral root canal treated tooth were included in the study. The evidence was searched in PubMed, EMBASE, Medline, Cochrane and Scopus. No language barrier was imposed. From analysis it found that there was no difference in amount of root resorption between vital and contralateral tooth. But, degree of resorption was determined by the intensity of force applied, force application technique (continuous or interrupted), duration of force application and direction of force application. Form the above fact it can be concluded that root canal treated tooth can be moved equal distance as vital contralateral tooth.Orthodontic Journal of Nepal, Vol. 6 No. 1, June 2016, pp.41-44

An Experimental Study on the Macroscopic Findings of Ligature Marks Using a Murine Model

Macroscopic findings of ligature marks are mainly affected by the characteristics of the ligature, the severity of external forces acting on the ligature, and the period that the neck has been pressed by the ligature. Therefore, the appearances of ligature marks formed by ligatures with the same characteristics differ depending on 2 factors: force and time. To examine which of these factors contributes more significantly to the macroscopic findings of ligature marks, a semiquantitative and experimental study using a murine model was performed. We experimentally made artificial ligature marks by hanging different sized weights using a vinyl band on dehaired legs of rats for different periods, both intravitally and postmortemly.After weights of 1, 3, and 6 kg with vinyl bands were hung on the legs of rats for 0 to 24 hours, the depths of ligature marks were semiquantitatively evaluated in 4 grades. Macroscopic examination and statistical analysis revealed that the appearance of ligature marks is not affected by whether they are formed intravitally or postmortemly but that it is equally affected by the severity of force and the duration of force application. We believe that the results of this study will be helpful to determine the characteristics of ligature and the period of hanging or strangulation time in the practice of forensic medicine.

Effect of using poles on foot–ground kinetics during stance phase in trail running

The aim of this study was to investigate the effect of using poles on foot–ground interaction during trail running with slopes of varying incline. Ten runners ran on a loop track representative of a trail running field situation with uphill (+9°), level and downhill (−6°) sections at fixed speed (3.2 m.s−1). Experimental conditions included running with (WP) and without (NP) the use of poles for each of the three slopes. Several quantitative and temporal foot–ground interaction parameters were calculated from plantar pressure data measured with a portable device. Using poles induced a decrease in plantar pressure intensity even when the running velocity stayed constant. However, the localisation and the magnitude of this decrease depended on the slope situations. During WP level running, regional analysis of the foot highlighted a decrease of the force time integral (FTI) for absolute (FTIabs; −12.6%; P<0.05) and relative values (FTIrel; −14.3%; P<0.05) in the medial forefoot region. FTIabs (−14.2%; P<0.05) and duration of force application (Δt; −13.5%; P<0.05) also decreased in the medial heel region when WP downhill running. These results support a facilitating effect of pole use for propulsion during level running and for the absorption phase during downhill running.

Physical properties of root cementum: Part 16. Comparisons of root resorption and resorption craters after the application of light and heavy continuous and controlled orthodontic forces for 4, 8, and 12 weeks

Orthodontic force duration can affect the severity of root resorption. The aim of this clinical study was to investigate the amounts of root resorption volumetrically after the application of controlled light and heavy forces in the buccal direction for 4, 8, and 12 weeks. The sample consisted of 54 maxillary first premolars in 36 patients (mean age, 14.9 years; 21 girls, 15 boys) who required first premolar extractions as part of their orthodontic treatment. The teeth were allocated into 3 groups that varied in the duration of force application: 4, 8, or 12 weeks. The right or left first premolars were randomly selected to receive 2 levels of forces. A light buccally directed orthodontic force of 25 g was applied to the experimental tooth on 1 side, while a heavy orthodontic force of 225 g was applied on the contralateral premolar. At the end of the experimental period, the teeth were extracted and scanned with the microcomputed-tomography x-ray system. Resorption crater analysis was performed with specially designed software for direct volumetric measurements. Significant differences in the extent of root resorption were found between 4, 8, and 12 weeks of force application (P <0.001), with substantially more severe resorption in the longer force duration groups. The light force produced significantly less root resorption than did the heavy force. After 4, 8, or 12 weeks of buccally directed orthodontic forces applied on the maxillary first premolars, the volumes of root resorption craters were found to be related to the duration and the magnitude of the forces.

Mini-implants in orthodontics: A systematic review of the literature

In this article, we systematically reviewed the literature to quantify success and complications encountered with the use of mini-implants for orthodontic anchorage, and to analyze factors associated with success or failure. Computerized and manual searches were conducted up to March 31, 2008, for clinical studies that addressed these objectives. The selection criteria required that these studies (1) reported the success rates of mini-implants on samples sizes of 10 implants or more, (2) gave a definition of success, (3) used implants with a diameter smaller than 2.5 mm, and (4) applied forces for a minimum duration of 3 months. Factors associated with implant success were accepted only if potentially influencing variables were controlled. The Cochrane Handbook for Systematic Reviews of Interventions was used as the guideline for this article. Nineteen reports met the inclusion criteria, but definitions of success, duration of force application, and quality of the methodology of these studies varied widely. Rates of primary outcomes ranged from 0% to 100%, but most articles reported success rates greater than 80% if mobile and displaced implants were included as successful. Adverse effects of miniscrews included biologic damage, inflammation, and pain and discomfort. Only a few articles reported negative outcomes. All proposed correlations between clinical success and specific variables such as implant, patient, location, surgery, orthodontic, and implant-maintenance factors were rejected because they did not meet the selection criteria for controlling those variables. Mini-implants can be used as temporary anchorage devices, but research in this field is still in its infancy. Interpretation of findings was conditioned by lack of clarity and poor methodology of most studies. Questions concerning patient acceptability, rate and severity of adverse effects of miniscrews, and variables that influenced success remain unanswered. This article includes a guideline for future studies of these issues, based on specific definitions of primary and secondary outcomes correlated with specific operational variables.

Editor's Summary, Q & A, Reviewer's Critique: Mini-implants in orthodontics: A systematic review of the literature

Introduction In this article, we systematically reviewed the literature to quantify success and complications encountered with the use of mini-implants for orthodontic anchorage, and to analyze factors associated with success or failure. Methods Computerized and manual searches were conducted up to March 31, 2008, for clinical studies that addressed these objectives. The selection criteria required that these studies (1) reported the success rates of mini-implants on samples sizes of 10 implants or more, (2) gave a definition of success, (3) used implants with a diameter smaller than 2.5 mm, and (4) applied forces for a minimum duration of 3 months. Factors associated with implant success were accepted only if potentially influencing variables were controlled. The Cochrane Handbook for Systematic Reviews of Interventions was used as the guideline for this article. Results Nineteen reports met the inclusion criteria, but definitions of success, duration of force application, and quality of the methodology of these studies varied widely. Rates of primary outcomes ranged from 0% to 100%, but most articles reported success rates greater than 80% if mobile and displaced implants were included as successful. Adverse effects of miniscrews included biologic damage, inflammation, and pain and discomfort. Only a few articles reported negative outcomes. All proposed correlations between clinical success and specific variables such as implant, patient, location, surgery, orthodontic, and implant-maintenance factors were rejected because they did not meet the selection criteria for controlling those variables. Conclusions Mini-implants can be used as temporary anchorage devices, but research in this field is still in its infancy. Interpretation of findings was conditioned by lack of clarity and poor methodology of most studies. Questions concerning patient acceptability, rate and severity of adverse effects of miniscrews, and variables that influenced success remain unanswered. This article includes a guideline for future studies of these issues, based on specific definitions of primary and secondary outcomes correlated with specific operational variables.

Effect of 8-hour intermittent orthodontic force on osteoclasts and root resorption

<h3>Introduction</h3> The duration of force application is an important factor in optimizing orthodontic tooth movement with less root resorption. It has previously been shown that the amount of tooth movement achieved by 8-hour intermittent force application exceeds what would be the expected by the duration. The purpose of this study was to compare osteoclast recruitment and the extent of root resorption in response to an 8-hour intermittent force regimen with those from a continuous force. <h3>Methods</h3> Rat molars (n = 110) were assigned to 3 groups: 8-hour intermittent force, continuous force, and control. A coil spring that exerted 40 cN of force was suspended between the maxillary first molars and the incisors. At 1, 3, 5, 7, and 14 days, osteoclast numbers, osteoclast surfaces, and root resorption were quantified histomorphometrically in the undecalcified sections and statistically analyzed. <h3>Results</h3> At the mesial sites, osteoclast numbers in the intermittent force group were 100.5% of the continuous force group, and osteoclast surfaces in the intermittent force group were 68.2% of the continuous force group. At the mesial sites, root resorption of the intermittent group was approximately 30.0% of the continuous group (<i>P</i> <0.01). <h3>Conclusions</h3> Our results show that an 8-hour intermittent force efficiently recruits osteoclasts while causing minimal root resorption.

Effect of 8-hour intermittent orthodontic force on osteoclasts and root resorption

The duration of force application is an important factor in optimizing orthodontic tooth movement with less root resorption. It has previously been shown that the amount of tooth movement achieved by 8-hour intermittent force application exceeds what would be the expected by the duration. The purpose of this study was to compare osteoclast recruitment and the extent of root resorption in response to an 8-hour intermittent force regimen with those from a continuous force. Rat molars (n = 110) were assigned to 3 groups: 8-hour intermittent force, continuous force, and control. A coil spring that exerted 40 cN of force was suspended between the maxillary first molars and the incisors. At 1, 3, 5, 7, and 14 days, osteoclast numbers, osteoclast surfaces, and root resorption were quantified histomorphometrically in the undecalcified sections and statistically analyzed. At the mesial sites, osteoclast numbers in the intermittent force group were 100.5% of the continuous force group, and osteoclast surfaces in the intermittent force group were 68.2% of the continuous force group. At the mesial sites, root resorption of the intermittent group was approximately 30.0% of the continuous group (P <0.01). Our results show that an 8-hour intermittent force efficiently recruits osteoclasts while causing minimal root resorption.

Intermittent 8-hour activation in orthodontic molar movement

The duration of force application is an important factor, together with the magnitude of force, in stimulating osteoclast recruitment in the periodontal ligament. The purpose of this study was to examine the effect of duration of intermittent versus continuous force application on the amount of molar movement in rats. Seventy-six molars of 38 rats were randomly assigned to continuous, intermittent, and sham-operation groups. A calibrated nickel-titanium coil spring exerting 40 g of initial force was suspended between the maxillary first molars and the incisors for 13 days. In the intermittent group, the coil spring was suspended for 8 hours per day. Tooth movement was measured on radiographs. There was no significant difference in the initial tooth displacement between the continuous-force and intermittent-force groups. Tooth movement at day 7 was significantly ( P < .05) less in the intermittent group than in the continuous group. Actual mesial tooth movement and total tooth movement in the intermittent group were 65.9% and 73.7%, respectively, of those in the continuous group. Our results suggest that the amount of tooth movement in response to intermittent force is less than that in response to continuous force, but that the amount of tooth movement achieved by intermittent force exceeds the expected value predicted by the duration (33.3%) of force application.

Canine retraction with rare earth magnets: An investigation into the validity of the constant force hypothesis

The objective of this study was to test the hypothesis that a prolonged constant force provides more effective tooth movement than an impulsive force of short duration. Six human subjects were selected, the main criterion being a need for extraction of their upper first premolars. Canine retraction on these subjects was executed on one side with the application of a force rapidly declining in magnitude, produced by a vertical loop, and on the other side with the application of a relatively constant force. This type of force was achieved by a similar vertical loop which was constantly activated by three parylene-coated neodymium-iron-boron (Nd 2Fe 14P) block magnets. The vertical loop on the control side was reactivated 6 weeks after the initial activation. No reactivation was necessary on the experimental side for the duration of the experiment. The rate of tooth movement on the two sides was compared over a period of 3 months, on the basis of maxillary impressions taken at frequent intervals during the course of the study. The canines retracted with a constant force moved statistically significantly more than the control canines ( p < 0.05) during the experimental period. The average differences in the mean rates of tooth movement between the two sides were in the order of 2:1 in favor of the experimental side. There were no statistically significant differences in the changes of angulation (tipping) or rotation about the y axis between the two sides. The duration of force application seems to be a critical factor in regulating rate of tooth movement. Conversely, magnitude of the applied force did not appear to be of primary significance. (Am J Orthod Dentofac Orthop 1996;109:489-95.)

Continuous versus intermittent extraoral traction: An experimental study

E xtraoral traction to the maxilla has proved to be clinically effective in the correction of anteroposterior discrepancies in occlusion. Recently, the effect of such traction on the bones of the craniofacial complex has also been recognized.5y i, 9, I*, 13, l6 In all previous experimental studies, with one exception,16 extraoral traction to the maxillas of monkeys has been achieved with continuously applied force (24 hours per day). However, the intermittent application of ext,raoral traction is a common clinical procedure, and investigation is indicated to compare different force-application schedules. This article will describe the treatment effects and the posttreatment stability of extraoral high-pull traction applied to the maxilla of Macaca nemestrina on an intermittent versus a continuous schedule. Duration of force application is the independent variable of study.